Shinella sp. MEC087

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Shinella

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5159 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y8QN79|A0A4Y8QN79_9RHIZ Uncharacterized protein OS=Shinella sp. MEC087 OX=1967501 GN=B5M44_04845 PE=4 SV=1
MM1 pKa = 6.93VAAEE5 pKa = 4.66PEE7 pKa = 3.86PLEE10 pKa = 4.03YY11 pKa = 11.01VRR13 pKa = 11.84VCDD16 pKa = 4.21AFGTGFFYY24 pKa = 10.4IPGTEE29 pKa = 3.91TCLKK33 pKa = 9.25ISGYY37 pKa = 9.95VRR39 pKa = 11.84FQTDD43 pKa = 3.44FGRR46 pKa = 11.84DD47 pKa = 3.17EE48 pKa = 5.06FGNSDD53 pKa = 2.85WSSWTRR59 pKa = 11.84ARR61 pKa = 11.84VAFDD65 pKa = 3.56AKK67 pKa = 10.93SDD69 pKa = 4.02TEE71 pKa = 4.62LGTLTSFIAIHH82 pKa = 6.41GEE84 pKa = 4.09SGHH87 pKa = 6.52GGLGLDD93 pKa = 3.79IEE95 pKa = 4.39NAEE98 pKa = 4.12LVGLGSGASSDD109 pKa = 4.03FYY111 pKa = 10.9IDD113 pKa = 2.96EE114 pKa = 4.91AYY116 pKa = 9.83IEE118 pKa = 4.34LGGFRR123 pKa = 11.84VGTFLNWWDD132 pKa = 3.47NDD134 pKa = 4.48LIGEE138 pKa = 4.47TEE140 pKa = 4.01TASNITRR147 pKa = 11.84FTSVRR152 pKa = 11.84YY153 pKa = 10.0VYY155 pKa = 10.85DD156 pKa = 3.71GGTFRR161 pKa = 11.84AGLSVDD167 pKa = 4.96EE168 pKa = 4.96IPDD171 pKa = 3.64YY172 pKa = 11.45TDD174 pKa = 3.97LLTNATYY181 pKa = 10.8HH182 pKa = 6.14NGFGVSGMVAATLGGVNFEE201 pKa = 4.89LLGAYY206 pKa = 9.65DD207 pKa = 4.58DD208 pKa = 4.2STEE211 pKa = 4.06EE212 pKa = 4.03GAVRR216 pKa = 11.84AWLSAEE222 pKa = 3.98VGPGTFEE229 pKa = 5.42LMGLYY234 pKa = 10.49ASGPNAYY241 pKa = 9.82YY242 pKa = 10.6DD243 pKa = 3.51AAEE246 pKa = 4.04WSVAADD252 pKa = 3.51YY253 pKa = 11.22SFKK256 pKa = 10.55ATEE259 pKa = 4.59KK260 pKa = 10.32LTITPGAQYY269 pKa = 10.53FGDD272 pKa = 3.7YY273 pKa = 11.23GLIDD277 pKa = 5.1GNDD280 pKa = 3.18AWRR283 pKa = 11.84VGLTVDD289 pKa = 3.64YY290 pKa = 11.2AIVDD294 pKa = 3.84NLALKK299 pKa = 9.44ATVNYY304 pKa = 8.12TDD306 pKa = 5.2PDD308 pKa = 3.85NEE310 pKa = 4.64DD311 pKa = 3.81DD312 pKa = 4.32FVTGFLRR319 pKa = 11.84LQRR322 pKa = 11.84NFF324 pKa = 3.24

Molecular weight: 35.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y8QMR8|A0A4Y8QMR8_9RHIZ RpiR family transcriptional regulator OS=Shinella sp. MEC087 OX=1967501 GN=B5M44_05715 PE=4 SV=1
MM1 pKa = 7.74SKK3 pKa = 8.95RR4 pKa = 11.84TYY6 pKa = 10.27QPSKK10 pKa = 9.73LVRR13 pKa = 11.84KK14 pKa = 9.15RR15 pKa = 11.84RR16 pKa = 11.84HH17 pKa = 4.42GFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 10.22GGRR29 pKa = 11.84KK30 pKa = 9.06VLSARR35 pKa = 11.84RR36 pKa = 11.84ARR38 pKa = 11.84GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.99

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5159

0

5159

1578796

28

2833

306.0

33.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.467 ± 0.045

0.798 ± 0.01

5.764 ± 0.029

5.766 ± 0.031

3.853 ± 0.023

8.535 ± 0.033

1.986 ± 0.016

5.571 ± 0.022

3.573 ± 0.025

9.887 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.553 ± 0.017

2.726 ± 0.02

4.801 ± 0.026

2.933 ± 0.021

6.827 ± 0.032

5.473 ± 0.026

5.433 ± 0.021

7.503 ± 0.027

1.257 ± 0.014

2.293 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski