Capybara microvirus Cap1_SP_61
Average proteome isoelectric point is 6.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W3X3|A0A4P8W3X3_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_61 OX=2584786 PE=4 SV=1
MM1 pKa = 7.93 RR2 pKa = 11.84 FTNNFVFAHH11 pKa = 5.57 PVFLNSNNTPTAQGASLTPAQIAKK35 pKa = 9.06 ATMEE39 pKa = 5.21 GIPISPQGLSTEE51 pKa = 4.46 QYY53 pKa = 10.75 FEE55 pKa = 4.92 GNTGDD60 pKa = 3.45 SMYY63 pKa = 10.34 IAPQFMRR70 pKa = 11.84 GVDD73 pKa = 5.52 LNDD76 pKa = 2.93 AWNIVQDD83 pKa = 3.82 NKK85 pKa = 11.18 KK86 pKa = 10.32 SFNSLTISEE95 pKa = 4.77 NGNDD99 pKa = 3.36
Molecular weight: 10.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.642
IPC2_protein 4.647
IPC_protein 4.431
Toseland 4.266
ProMoST 4.393
Dawson 4.406
Bjellqvist 4.635
Wikipedia 4.317
Rodwell 4.279
Grimsley 4.19
Solomon 4.393
Lehninger 4.342
Nozaki 4.52
DTASelect 4.698
Thurlkill 4.304
EMBOSS 4.329
Sillero 4.558
Patrickios 4.024
IPC_peptide 4.393
IPC2_peptide 4.533
IPC2.peptide.svr19 4.523
Protein with the highest isoelectric point:
>tr|A0A4V1FVM8|A0A4V1FVM8_9VIRU Replication initiator protein OS=Capybara microvirus Cap1_SP_61 OX=2584786 PE=4 SV=1
MM1 pKa = 7.47 ICLSPKK7 pKa = 9.58 YY8 pKa = 9.8 SLNYY12 pKa = 9.79 RR13 pKa = 11.84 ILGKK17 pKa = 10.3 IMPNIVGYY25 pKa = 10.21 SIEE28 pKa = 4.07 NQVYY32 pKa = 7.41 FTKK35 pKa = 10.39 PSYY38 pKa = 10.58 RR39 pKa = 11.84 SLQCALDD46 pKa = 3.77 DD47 pKa = 5.11 LIAFGSSSYY56 pKa = 10.7 KK57 pKa = 10.62 VLVTGYY63 pKa = 10.32 YY64 pKa = 10.52 DD65 pKa = 3.49 GLSVVNYY72 pKa = 8.22 PVSPFIVTPCGHH84 pKa = 6.85 CASCLKK90 pKa = 10.64 AKK92 pKa = 9.96 QLNKK96 pKa = 6.35 TTCMQLEE103 pKa = 4.3 ALNYY107 pKa = 7.95 RR108 pKa = 11.84 QPLFVTLTYY117 pKa = 10.85 ADD119 pKa = 4.45 EE120 pKa = 4.82 PPSGVNVKK128 pKa = 10.16 DD129 pKa = 3.35 VQNFLKK135 pKa = 10.64 RR136 pKa = 11.84 LRR138 pKa = 11.84 TNLKK142 pKa = 9.73 RR143 pKa = 11.84 KK144 pKa = 10.23 GYY146 pKa = 9.64 DD147 pKa = 2.98 EE148 pKa = 5.53 PIRR151 pKa = 11.84 YY152 pKa = 8.78 HH153 pKa = 5.94 CTAEE157 pKa = 4.25 YY158 pKa = 10.69 GSKK161 pKa = 9.29 TYY163 pKa = 10.2 RR164 pKa = 11.84 PHH166 pKa = 5.22 YY167 pKa = 10.08 HH168 pKa = 6.95 LIIYY172 pKa = 8.21 NMPNEE177 pKa = 4.25 EE178 pKa = 4.47 FFDD181 pKa = 4.1 LPAFGSYY188 pKa = 9.96 GAGYY192 pKa = 10.35 AYY194 pKa = 10.51 DD195 pKa = 4.53 DD196 pKa = 5.51 FIRR199 pKa = 11.84 SCWKK203 pKa = 10.52 LGLTHH208 pKa = 6.66 SRR210 pKa = 11.84 LVSDD214 pKa = 4.15 VAALRR219 pKa = 11.84 YY220 pKa = 8.0 CAKK223 pKa = 9.35 YY224 pKa = 7.8 TAKK227 pKa = 10.36 CSSILKK233 pKa = 9.89 GKK235 pKa = 9.8 NPPFMLQSTKK245 pKa = 10.98 YY246 pKa = 9.45 GGLGSKK252 pKa = 9.77 VYY254 pKa = 10.79 KK255 pKa = 10.68 NLANEE260 pKa = 4.53 FLTQKK265 pKa = 10.88 NIVNVNGKK273 pKa = 8.14 QVSLPLNAYY282 pKa = 7.86 FLRR285 pKa = 11.84 KK286 pKa = 9.47 VLPSAQQCVGYY297 pKa = 9.86 YY298 pKa = 8.05 WLRR301 pKa = 11.84 IMKK304 pKa = 10.16 SLEE307 pKa = 4.07 LCGKK311 pKa = 9.86 LPPLHH316 pKa = 6.83 PFYY319 pKa = 10.72 IDD321 pKa = 4.18 LLHH324 pKa = 7.0 HH325 pKa = 5.74 YY326 pKa = 10.15 QPAKK330 pKa = 10.74 DD331 pKa = 3.48 SAIYY335 pKa = 10.55 ADD337 pKa = 4.03 IVILSGQILKK347 pKa = 8.2 TCYY350 pKa = 9.79 YY351 pKa = 9.83 RR352 pKa = 11.84 GLISSRR358 pKa = 11.84 FTDD361 pKa = 5.14 FSAQKK366 pKa = 9.62 TIEE369 pKa = 3.97 FLNFKK374 pKa = 10.53 HH375 pKa = 6.99 SFLQNLPYY383 pKa = 10.29 QSPAEE388 pKa = 3.96 PKK390 pKa = 10.06 ILEE393 pKa = 4.11 NYY395 pKa = 8.57 IAQNQLDD402 pKa = 3.89 RR403 pKa = 11.84 QKK405 pKa = 10.82 EE406 pKa = 4.11 KK407 pKa = 11.15 EE408 pKa = 3.99 IMM410 pKa = 3.8
Molecular weight: 46.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.691
IPC2_protein 8.653
IPC_protein 8.492
Toseland 8.829
ProMoST 8.916
Dawson 9.282
Bjellqvist 9.238
Wikipedia 9.472
Rodwell 9.385
Grimsley 9.311
Solomon 9.311
Lehninger 9.268
Nozaki 9.268
DTASelect 9.121
Thurlkill 9.165
EMBOSS 9.385
Sillero 9.355
Patrickios 3.071
IPC_peptide 9.297
IPC2_peptide 8.112
IPC2.peptide.svr19 7.83
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
1873
68
774
267.6
30.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.46 ± 0.832
1.548 ± 0.506
4.645 ± 0.379
4.698 ± 1.014
4.645 ± 0.774
5.392 ± 0.353
1.762 ± 0.408
6.941 ± 0.52
5.072 ± 0.834
8.916 ± 0.943
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.403 ± 0.255
7.208 ± 0.702
4.592 ± 0.69
4.592 ± 0.494
4.164 ± 0.295
8.97 ± 0.488
6.407 ± 0.661
5.392 ± 0.676
0.481 ± 0.17
5.713 ± 0.972
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here