Amino acid dipepetide frequency for Bacillus phage 049ML001

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.35AlaAla: 8.35 ± 1.581
0.205AlaCys: 0.205 ± 0.116
4.175AlaAsp: 4.175 ± 0.583
6.023AlaGlu: 6.023 ± 0.958
3.217AlaPhe: 3.217 ± 0.566
4.517AlaGly: 4.517 ± 0.443
1.095AlaHis: 1.095 ± 0.303
4.244AlaIle: 4.244 ± 0.656
5.407AlaLys: 5.407 ± 0.788
6.229AlaLeu: 6.229 ± 0.55
1.985AlaMet: 1.985 ± 0.314
2.943AlaAsn: 2.943 ± 0.509
1.78AlaPro: 1.78 ± 0.452
2.738AlaGln: 2.738 ± 0.453
3.285AlaArg: 3.285 ± 0.47
4.038AlaSer: 4.038 ± 0.591
3.559AlaThr: 3.559 ± 0.672
4.86AlaVal: 4.86 ± 0.806
1.369AlaTrp: 1.369 ± 0.287
2.806AlaTyr: 2.806 ± 0.466
0.0AlaXaa: 0.0 ± 0.0
Cys
0.684CysAla: 0.684 ± 0.246
0.205CysCys: 0.205 ± 0.162
0.616CysAsp: 0.616 ± 0.238
0.616CysGlu: 0.616 ± 0.232
0.205CysPhe: 0.205 ± 0.12
0.616CysGly: 0.616 ± 0.311
0.342CysHis: 0.342 ± 0.155
0.411CysIle: 0.411 ± 0.225
0.616CysLys: 0.616 ± 0.24
0.205CysLeu: 0.205 ± 0.159
0.0CysMet: 0.0 ± 0.0
0.479CysAsn: 0.479 ± 0.215
0.274CysPro: 0.274 ± 0.141
0.274CysGln: 0.274 ± 0.134
0.342CysArg: 0.342 ± 0.116
0.137CysSer: 0.137 ± 0.094
0.068CysThr: 0.068 ± 0.061
0.548CysVal: 0.548 ± 0.225
0.0CysTrp: 0.0 ± 0.0
0.137CysTyr: 0.137 ± 0.096
0.0CysXaa: 0.0 ± 0.0
Asp
4.586AspAla: 4.586 ± 0.72
0.89AspCys: 0.89 ± 0.237
4.517AspAsp: 4.517 ± 0.743
5.339AspGlu: 5.339 ± 0.728
2.875AspPhe: 2.875 ± 0.4
5.065AspGly: 5.065 ± 0.595
0.958AspHis: 0.958 ± 0.253
4.312AspIle: 4.312 ± 0.709
4.381AspLys: 4.381 ± 0.572
4.449AspLeu: 4.449 ± 0.548
1.3AspMet: 1.3 ± 0.272
3.149AspAsn: 3.149 ± 0.736
1.985AspPro: 1.985 ± 0.442
1.164AspGln: 1.164 ± 0.342
2.533AspArg: 2.533 ± 0.374
3.149AspSer: 3.149 ± 0.438
3.354AspThr: 3.354 ± 0.491
3.354AspVal: 3.354 ± 0.426
1.027AspTrp: 1.027 ± 0.294
2.464AspTyr: 2.464 ± 0.361
0.0AspXaa: 0.0 ± 0.0
Glu
4.791GluAla: 4.791 ± 0.564
0.411GluCys: 0.411 ± 0.168
2.943GluAsp: 2.943 ± 0.487
7.529GluGlu: 7.529 ± 0.97
3.149GluPhe: 3.149 ± 0.383
5.681GluGly: 5.681 ± 0.598
1.711GluHis: 1.711 ± 0.424
5.065GluIle: 5.065 ± 0.659
6.023GluLys: 6.023 ± 1.014
8.487GluLeu: 8.487 ± 0.813
3.833GluMet: 3.833 ± 0.594
4.791GluAsn: 4.791 ± 0.569
2.738GluPro: 2.738 ± 0.338
3.628GluGln: 3.628 ± 0.6
4.723GluArg: 4.723 ± 0.515
3.833GluSer: 3.833 ± 0.614
3.149GluThr: 3.149 ± 0.437
5.133GluVal: 5.133 ± 0.785
1.232GluTrp: 1.232 ± 0.369
3.285GluTyr: 3.285 ± 0.488
0.0GluXaa: 0.0 ± 0.0
Phe
3.08PheAla: 3.08 ± 0.428
0.342PheCys: 0.342 ± 0.208
2.669PheAsp: 2.669 ± 0.436
3.149PheGlu: 3.149 ± 0.541
1.78PhePhe: 1.78 ± 0.291
2.875PheGly: 2.875 ± 0.4
0.616PheHis: 0.616 ± 0.201
2.601PheIle: 2.601 ± 0.51
3.559PheLys: 3.559 ± 0.509
2.943PheLeu: 2.943 ± 0.51
1.369PheMet: 1.369 ± 0.278
1.916PheAsn: 1.916 ± 0.393
0.89PhePro: 0.89 ± 0.211
1.506PheGln: 1.506 ± 0.332
1.164PheArg: 1.164 ± 0.27
1.916PheSer: 1.916 ± 0.322
3.08PheThr: 3.08 ± 0.433
2.122PheVal: 2.122 ± 0.417
0.684PheTrp: 0.684 ± 0.208
1.437PheTyr: 1.437 ± 0.267
0.0PheXaa: 0.0 ± 0.0
Gly
5.202GlyAla: 5.202 ± 0.603
0.684GlyCys: 0.684 ± 0.257
3.491GlyAsp: 3.491 ± 0.513
5.749GlyGlu: 5.749 ± 0.72
2.669GlyPhe: 2.669 ± 0.403
6.913GlyGly: 6.913 ± 0.671
1.3GlyHis: 1.3 ± 0.381
4.244GlyIle: 4.244 ± 0.611
5.886GlyLys: 5.886 ± 0.651
4.038GlyLeu: 4.038 ± 0.573
2.122GlyMet: 2.122 ± 0.368
3.354GlyAsn: 3.354 ± 0.434
2.053GlyPro: 2.053 ± 0.378
3.012GlyGln: 3.012 ± 0.541
3.901GlyArg: 3.901 ± 0.566
4.86GlySer: 4.86 ± 0.684
4.586GlyThr: 4.586 ± 0.69
4.244GlyVal: 4.244 ± 0.529
0.342GlyTrp: 0.342 ± 0.155
3.765GlyTyr: 3.765 ± 0.528
0.0GlyXaa: 0.0 ± 0.0
His
0.89HisAla: 0.89 ± 0.391
0.137HisCys: 0.137 ± 0.094
1.711HisAsp: 1.711 ± 0.432
1.095HisGlu: 1.095 ± 0.309
0.821HisPhe: 0.821 ± 0.231
1.437HisGly: 1.437 ± 0.367
0.411HisHis: 0.411 ± 0.192
1.027HisIle: 1.027 ± 0.263
1.369HisLys: 1.369 ± 0.275
1.232HisLeu: 1.232 ± 0.306
0.684HisMet: 0.684 ± 0.268
0.616HisAsn: 0.616 ± 0.212
0.753HisPro: 0.753 ± 0.221
0.411HisGln: 0.411 ± 0.17
1.027HisArg: 1.027 ± 0.279
1.027HisSer: 1.027 ± 0.249
0.548HisThr: 0.548 ± 0.154
1.095HisVal: 1.095 ± 0.252
0.479HisTrp: 0.479 ± 0.271
0.548HisTyr: 0.548 ± 0.215
0.0HisXaa: 0.0 ± 0.0
Ile
3.422IleAla: 3.422 ± 0.555
0.205IleCys: 0.205 ± 0.106
4.244IleAsp: 4.244 ± 0.571
5.544IleGlu: 5.544 ± 0.702
2.053IlePhe: 2.053 ± 0.39
4.038IleGly: 4.038 ± 0.501
0.821IleHis: 0.821 ± 0.253
4.586IleIle: 4.586 ± 0.535
6.845IleLys: 6.845 ± 0.661
4.86IleLeu: 4.86 ± 0.548
2.259IleMet: 2.259 ± 0.397
3.559IleAsn: 3.559 ± 0.46
2.396IlePro: 2.396 ± 0.497
2.533IleGln: 2.533 ± 0.393
2.738IleArg: 2.738 ± 0.476
4.586IleSer: 4.586 ± 0.553
4.517IleThr: 4.517 ± 0.672
4.381IleVal: 4.381 ± 0.506
0.753IleTrp: 0.753 ± 0.261
2.533IleTyr: 2.533 ± 0.43
0.0IleXaa: 0.0 ± 0.0
Lys
5.886LysAla: 5.886 ± 0.611
0.342LysCys: 0.342 ± 0.154
4.449LysAsp: 4.449 ± 0.546
7.666LysGlu: 7.666 ± 0.957
2.122LysPhe: 2.122 ± 0.411
5.476LysGly: 5.476 ± 0.631
1.916LysHis: 1.916 ± 0.375
5.544LysIle: 5.544 ± 0.73
8.282LysLys: 8.282 ± 1.125
5.339LysLeu: 5.339 ± 0.609
3.149LysMet: 3.149 ± 0.452
5.681LysAsn: 5.681 ± 0.72
2.601LysPro: 2.601 ± 0.501
2.533LysGln: 2.533 ± 0.458
4.791LysArg: 4.791 ± 0.679
4.312LysSer: 4.312 ± 0.844
4.86LysThr: 4.86 ± 0.726
4.86LysVal: 4.86 ± 0.56
1.095LysTrp: 1.095 ± 0.304
2.396LysTyr: 2.396 ± 0.456
0.0LysXaa: 0.0 ± 0.0
Leu
4.791LeuAla: 4.791 ± 0.602
0.616LeuCys: 0.616 ± 0.244
4.175LeuAsp: 4.175 ± 0.498
7.05LeuGlu: 7.05 ± 0.848
2.533LeuPhe: 2.533 ± 0.442
4.86LeuGly: 4.86 ± 0.668
1.3LeuHis: 1.3 ± 0.29
4.175LeuIle: 4.175 ± 0.589
6.708LeuLys: 6.708 ± 0.753
4.381LeuLeu: 4.381 ± 0.714
2.053LeuMet: 2.053 ± 0.409
3.696LeuAsn: 3.696 ± 0.488
2.464LeuPro: 2.464 ± 0.536
3.354LeuGln: 3.354 ± 0.649
3.765LeuArg: 3.765 ± 0.551
3.97LeuSer: 3.97 ± 0.623
4.723LeuThr: 4.723 ± 0.62
4.038LeuVal: 4.038 ± 0.55
0.684LeuTrp: 0.684 ± 0.213
2.396LeuTyr: 2.396 ± 0.455
0.0LeuXaa: 0.0 ± 0.0
Met
2.943MetAla: 2.943 ± 0.625
0.205MetCys: 0.205 ± 0.122
1.369MetAsp: 1.369 ± 0.297
1.711MetGlu: 1.711 ± 0.325
0.821MetPhe: 0.821 ± 0.215
1.711MetGly: 1.711 ± 0.322
0.411MetHis: 0.411 ± 0.165
2.122MetIle: 2.122 ± 0.347
2.669MetLys: 2.669 ± 0.436
1.095MetLeu: 1.095 ± 0.245
0.958MetMet: 0.958 ± 0.29
2.669MetAsn: 2.669 ± 0.355
1.3MetPro: 1.3 ± 0.268
1.437MetGln: 1.437 ± 0.279
1.711MetArg: 1.711 ± 0.371
2.327MetSer: 2.327 ± 0.412
1.848MetThr: 1.848 ± 0.339
0.89MetVal: 0.89 ± 0.284
0.548MetTrp: 0.548 ± 0.193
1.3MetTyr: 1.3 ± 0.348
0.0MetXaa: 0.0 ± 0.0
Asn
3.97AsnAla: 3.97 ± 0.767
0.411AsnCys: 0.411 ± 0.192
3.696AsnAsp: 3.696 ± 0.608
4.381AsnGlu: 4.381 ± 0.657
2.396AsnPhe: 2.396 ± 0.364
4.381AsnGly: 4.381 ± 0.693
0.821AsnHis: 0.821 ± 0.255
4.312AsnIle: 4.312 ± 0.798
3.765AsnLys: 3.765 ± 0.475
3.97AsnLeu: 3.97 ± 0.512
1.506AsnMet: 1.506 ± 0.293
3.08AsnAsn: 3.08 ± 0.564
2.875AsnPro: 2.875 ± 0.502
1.232AsnGln: 1.232 ± 0.28
2.259AsnArg: 2.259 ± 0.436
2.327AsnSer: 2.327 ± 0.344
2.669AsnThr: 2.669 ± 0.434
2.738AsnVal: 2.738 ± 0.542
0.548AsnTrp: 0.548 ± 0.172
1.643AsnTyr: 1.643 ± 0.362
0.0AsnXaa: 0.0 ± 0.0
Pro
2.327ProAla: 2.327 ± 0.455
0.137ProCys: 0.137 ± 0.098
2.533ProAsp: 2.533 ± 0.364
3.149ProGlu: 3.149 ± 0.443
1.78ProPhe: 1.78 ± 0.345
2.943ProGly: 2.943 ± 0.445
0.684ProHis: 0.684 ± 0.214
1.985ProIle: 1.985 ± 0.323
2.533ProLys: 2.533 ± 0.361
2.396ProLeu: 2.396 ± 0.362
0.684ProMet: 0.684 ± 0.171
1.232ProAsn: 1.232 ± 0.311
1.437ProPro: 1.437 ± 0.366
1.095ProGln: 1.095 ± 0.244
0.821ProArg: 0.821 ± 0.246
2.053ProSer: 2.053 ± 0.359
1.369ProThr: 1.369 ± 0.349
2.738ProVal: 2.738 ± 0.476
0.342ProTrp: 0.342 ± 0.136
1.232ProTyr: 1.232 ± 0.283
0.0ProXaa: 0.0 ± 0.0
Gln
2.943GlnAla: 2.943 ± 0.478
0.068GlnCys: 0.068 ± 0.065
2.122GlnAsp: 2.122 ± 0.428
2.327GlnGlu: 2.327 ± 0.378
0.958GlnPhe: 0.958 ± 0.249
1.711GlnGly: 1.711 ± 0.374
0.479GlnHis: 0.479 ± 0.199
1.643GlnIle: 1.643 ± 0.335
3.149GlnLys: 3.149 ± 0.511
3.491GlnLeu: 3.491 ± 0.493
1.232GlnMet: 1.232 ± 0.388
1.848GlnAsn: 1.848 ± 0.372
1.437GlnPro: 1.437 ± 0.29
1.574GlnGln: 1.574 ± 0.38
1.506GlnArg: 1.506 ± 0.334
2.053GlnSer: 2.053 ± 0.359
2.19GlnThr: 2.19 ± 0.394
2.259GlnVal: 2.259 ± 0.438
0.274GlnTrp: 0.274 ± 0.128
1.232GlnTyr: 1.232 ± 0.294
0.0GlnXaa: 0.0 ± 0.0
Arg
2.943ArgAla: 2.943 ± 0.511
0.548ArgCys: 0.548 ± 0.177
2.533ArgAsp: 2.533 ± 0.327
3.559ArgGlu: 3.559 ± 0.446
2.533ArgPhe: 2.533 ± 0.514
3.833ArgGly: 3.833 ± 0.526
0.821ArgHis: 0.821 ± 0.224
4.723ArgIle: 4.723 ± 0.699
4.038ArgLys: 4.038 ± 0.524
3.833ArgLeu: 3.833 ± 0.677
1.574ArgMet: 1.574 ± 0.309
1.916ArgAsn: 1.916 ± 0.331
1.232ArgPro: 1.232 ± 0.303
1.164ArgGln: 1.164 ± 0.275
2.601ArgArg: 2.601 ± 0.457
1.506ArgSer: 1.506 ± 0.327
3.217ArgThr: 3.217 ± 0.441
3.422ArgVal: 3.422 ± 0.496
0.684ArgTrp: 0.684 ± 0.258
1.985ArgTyr: 1.985 ± 0.405
0.0ArgXaa: 0.0 ± 0.0
Ser
4.791SerAla: 4.791 ± 0.617
0.411SerCys: 0.411 ± 0.154
3.901SerAsp: 3.901 ± 0.511
3.559SerGlu: 3.559 ± 0.584
2.396SerPhe: 2.396 ± 0.404
5.202SerGly: 5.202 ± 0.606
0.616SerHis: 0.616 ± 0.163
3.422SerIle: 3.422 ± 0.551
3.833SerLys: 3.833 ± 0.454
3.628SerLeu: 3.628 ± 0.632
1.78SerMet: 1.78 ± 0.375
1.848SerAsn: 1.848 ± 0.401
1.369SerPro: 1.369 ± 0.285
1.506SerGln: 1.506 ± 0.332
3.354SerArg: 3.354 ± 0.477
2.533SerSer: 2.533 ± 0.619
3.696SerThr: 3.696 ± 0.511
4.244SerVal: 4.244 ± 0.552
1.027SerTrp: 1.027 ± 0.235
1.916SerTyr: 1.916 ± 0.411
0.0SerXaa: 0.0 ± 0.0
Thr
4.928ThrAla: 4.928 ± 0.955
0.411ThrCys: 0.411 ± 0.198
3.696ThrAsp: 3.696 ± 0.574
4.381ThrGlu: 4.381 ± 0.581
3.354ThrPhe: 3.354 ± 0.423
4.928ThrGly: 4.928 ± 0.874
0.821ThrHis: 0.821 ± 0.238
4.038ThrIle: 4.038 ± 0.413
3.559ThrLys: 3.559 ± 0.575
3.422ThrLeu: 3.422 ± 0.487
1.232ThrMet: 1.232 ± 0.256
2.601ThrAsn: 2.601 ± 0.487
2.533ThrPro: 2.533 ± 0.403
1.643ThrGln: 1.643 ± 0.342
2.327ThrArg: 2.327 ± 0.453
2.875ThrSer: 2.875 ± 0.631
2.875ThrThr: 2.875 ± 0.461
4.381ThrVal: 4.381 ± 0.669
0.684ThrTrp: 0.684 ± 0.186
2.327ThrTyr: 2.327 ± 0.312
0.0ThrXaa: 0.0 ± 0.0
Val
3.149ValAla: 3.149 ± 0.498
0.137ValCys: 0.137 ± 0.095
3.97ValAsp: 3.97 ± 0.494
5.065ValGlu: 5.065 ± 0.693
2.053ValPhe: 2.053 ± 0.416
2.464ValGly: 2.464 ± 0.344
1.027ValHis: 1.027 ± 0.281
5.202ValIle: 5.202 ± 0.517
6.639ValLys: 6.639 ± 0.61
3.97ValLeu: 3.97 ± 0.507
1.3ValMet: 1.3 ± 0.305
4.038ValAsn: 4.038 ± 0.697
2.19ValPro: 2.19 ± 0.368
2.053ValGln: 2.053 ± 0.275
3.08ValArg: 3.08 ± 0.457
3.97ValSer: 3.97 ± 0.55
4.723ValThr: 4.723 ± 0.696
3.354ValVal: 3.354 ± 0.451
1.643ValTrp: 1.643 ± 0.509
2.259ValTyr: 2.259 ± 0.403
0.0ValXaa: 0.0 ± 0.0
Trp
0.821TrpAla: 0.821 ± 0.219
0.137TrpCys: 0.137 ± 0.102
0.753TrpAsp: 0.753 ± 0.245
0.684TrpGlu: 0.684 ± 0.294
0.479TrpPhe: 0.479 ± 0.285
1.3TrpGly: 1.3 ± 0.219
0.479TrpHis: 0.479 ± 0.225
0.89TrpIle: 0.89 ± 0.212
1.095TrpLys: 1.095 ± 0.255
0.821TrpLeu: 0.821 ± 0.246
0.137TrpMet: 0.137 ± 0.103
1.574TrpAsn: 1.574 ± 0.535
0.137TrpPro: 0.137 ± 0.102
0.411TrpGln: 0.411 ± 0.138
0.89TrpArg: 0.89 ± 0.262
1.027TrpSer: 1.027 ± 0.218
0.548TrpThr: 0.548 ± 0.192
1.027TrpVal: 1.027 ± 0.246
0.068TrpTrp: 0.068 ± 0.072
0.753TrpTyr: 0.753 ± 0.19
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.327TyrAla: 2.327 ± 0.409
0.274TyrCys: 0.274 ± 0.115
3.285TyrAsp: 3.285 ± 0.541
3.628TyrGlu: 3.628 ± 0.695
1.506TyrPhe: 1.506 ± 0.268
2.464TyrGly: 2.464 ± 0.379
0.684TyrHis: 0.684 ± 0.184
2.327TyrIle: 2.327 ± 0.504
2.943TyrLys: 2.943 ± 0.5
2.943TyrLeu: 2.943 ± 0.417
0.821TyrMet: 0.821 ± 0.241
1.985TyrAsn: 1.985 ± 0.343
1.027TyrPro: 1.027 ± 0.294
1.232TyrGln: 1.232 ± 0.296
1.848TyrArg: 1.848 ± 0.383
2.533TyrSer: 2.533 ± 0.523
1.437TyrThr: 1.437 ± 0.309
2.601TyrVal: 2.601 ± 0.402
0.548TyrTrp: 0.548 ± 0.211
1.643TyrTyr: 1.643 ± 0.414
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 82 proteins (14611 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski