Streptococcus phage 8140
Average proteome isoelectric point is 5.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E8ZDX7|E8ZDX7_9CAUD Phage protein OS=Streptococcus phage 8140 OX=870474 PE=4 SV=1
MM1 pKa = 8.39 LMFNYY6 pKa = 10.01 DD7 pKa = 3.94 RR8 pKa = 11.84 DD9 pKa = 3.49 IMQPPEE15 pKa = 3.98 EE16 pKa = 4.61 RR17 pKa = 11.84 EE18 pKa = 4.03 EE19 pKa = 4.62 LDD21 pKa = 3.41 PADD24 pKa = 4.33 WIFSAGQWIYY34 pKa = 11.59 VGDD37 pKa = 4.05 CC38 pKa = 3.39
Molecular weight: 4.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.815
IPC2_protein 3.732
IPC_protein 3.567
Toseland 3.401
ProMoST 3.795
Dawson 3.592
Bjellqvist 3.757
Wikipedia 3.567
Rodwell 3.427
Grimsley 3.325
Solomon 3.528
Lehninger 3.49
Nozaki 3.745
DTASelect 3.897
Thurlkill 3.49
EMBOSS 3.567
Sillero 3.706
Patrickios 1.837
IPC_peptide 3.528
IPC2_peptide 3.668
IPC2.peptide.svr19 3.704
Protein with the highest isoelectric point:
>tr|E8ZDX1|E8ZDX1_9CAUD Phage protein OS=Streptococcus phage 8140 OX=870474 PE=4 SV=1
MM1 pKa = 7.05 QRR3 pKa = 11.84 RR4 pKa = 11.84 KK5 pKa = 10.26 IMNDD9 pKa = 3.85 FIKK12 pKa = 10.66 EE13 pKa = 3.54 IGLAILWMFLGYY25 pKa = 10.88 LLGEE29 pKa = 4.21 RR30 pKa = 11.84 STRR33 pKa = 11.84 GGQIRR38 pKa = 3.63
Molecular weight: 4.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.293
IPC2_protein 9.809
IPC_protein 10.906
Toseland 11.023
ProMoST 11.564
Dawson 11.067
Bjellqvist 10.877
Wikipedia 11.374
Rodwell 11.125
Grimsley 11.111
Solomon 11.345
Lehninger 11.286
Nozaki 10.994
DTASelect 10.877
Thurlkill 11.008
EMBOSS 11.447
Sillero 11.023
Patrickios 10.979
IPC_peptide 11.345
IPC2_peptide 10.014
IPC2.peptide.svr19 8.964
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
53
0
53
10477
38
1217
197.7
22.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.7 ± 0.684
0.687 ± 0.117
6.567 ± 0.296
8.132 ± 0.486
4.114 ± 0.284
6.309 ± 0.596
1.336 ± 0.172
6.634 ± 0.266
8.352 ± 0.391
8.123 ± 0.287
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.529 ± 0.194
5.355 ± 0.252
2.72 ± 0.231
4.295 ± 0.253
4.648 ± 0.357
6.042 ± 0.301
5.841 ± 0.273
6.624 ± 0.294
1.212 ± 0.153
3.78 ± 0.379
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here