Amino acid dipepetide frequency for Pectobacterium phage MA6

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.095AlaAla: 10.095 ± 1.494
1.053AlaCys: 1.053 ± 0.33
5.618AlaAsp: 5.618 ± 0.699
5.004AlaGlu: 5.004 ± 0.564
3.248AlaPhe: 3.248 ± 0.508
6.32AlaGly: 6.32 ± 0.822
0.966AlaHis: 0.966 ± 0.284
3.687AlaIle: 3.687 ± 0.663
5.706AlaLys: 5.706 ± 0.69
7.461AlaLeu: 7.461 ± 0.813
2.458AlaMet: 2.458 ± 0.563
5.004AlaAsn: 5.004 ± 0.604
3.336AlaPro: 3.336 ± 0.519
3.95AlaGln: 3.95 ± 0.645
5.004AlaArg: 5.004 ± 0.952
5.53AlaSer: 5.53 ± 0.97
3.423AlaThr: 3.423 ± 0.417
6.145AlaVal: 6.145 ± 0.855
0.79AlaTrp: 0.79 ± 0.392
2.985AlaTyr: 2.985 ± 0.53
0.0AlaXaa: 0.0 ± 0.0
Cys
0.79CysAla: 0.79 ± 0.282
0.176CysCys: 0.176 ± 0.143
0.614CysAsp: 0.614 ± 0.263
0.702CysGlu: 0.702 ± 0.23
0.527CysPhe: 0.527 ± 0.201
0.966CysGly: 0.966 ± 0.272
0.263CysHis: 0.263 ± 0.129
0.79CysIle: 0.79 ± 0.335
0.439CysLys: 0.439 ± 0.207
0.702CysLeu: 0.702 ± 0.22
0.088CysMet: 0.088 ± 0.087
0.088CysAsn: 0.088 ± 0.085
0.439CysPro: 0.439 ± 0.2
0.263CysGln: 0.263 ± 0.144
1.229CysArg: 1.229 ± 0.564
0.263CysSer: 0.263 ± 0.118
0.351CysThr: 0.351 ± 0.188
0.79CysVal: 0.79 ± 0.281
0.439CysTrp: 0.439 ± 0.21
0.614CysTyr: 0.614 ± 0.259
0.0CysXaa: 0.0 ± 0.0
Asp
5.881AspAla: 5.881 ± 0.633
0.527AspCys: 0.527 ± 0.205
2.985AspAsp: 2.985 ± 0.34
3.687AspGlu: 3.687 ± 0.577
2.985AspPhe: 2.985 ± 0.568
6.935AspGly: 6.935 ± 0.583
0.702AspHis: 0.702 ± 0.19
3.072AspIle: 3.072 ± 0.509
4.301AspLys: 4.301 ± 0.498
3.16AspLeu: 3.16 ± 0.533
2.019AspMet: 2.019 ± 0.377
2.546AspAsn: 2.546 ± 0.459
2.546AspPro: 2.546 ± 0.407
1.756AspGln: 1.756 ± 0.335
2.897AspArg: 2.897 ± 0.525
4.565AspSer: 4.565 ± 0.58
3.423AspThr: 3.423 ± 0.696
4.038AspVal: 4.038 ± 0.583
0.702AspTrp: 0.702 ± 0.23
2.721AspTyr: 2.721 ± 0.495
0.0AspXaa: 0.0 ± 0.0
Glu
5.969GluAla: 5.969 ± 0.538
0.878GluCys: 0.878 ± 0.346
4.301GluAsp: 4.301 ± 0.564
4.828GluGlu: 4.828 ± 0.866
2.985GluPhe: 2.985 ± 0.423
5.179GluGly: 5.179 ± 0.704
1.58GluHis: 1.58 ± 0.398
3.072GluIle: 3.072 ± 0.441
2.897GluLys: 2.897 ± 0.575
4.828GluLeu: 4.828 ± 0.514
1.492GluMet: 1.492 ± 0.296
2.37GluAsn: 2.37 ± 0.416
1.756GluPro: 1.756 ± 0.368
2.282GluGln: 2.282 ± 0.637
3.775GluArg: 3.775 ± 0.579
4.565GluSer: 4.565 ± 0.671
4.213GluThr: 4.213 ± 0.62
3.423GluVal: 3.423 ± 0.548
0.79GluTrp: 0.79 ± 0.346
3.16GluTyr: 3.16 ± 0.538
0.0GluXaa: 0.0 ± 0.0
Phe
2.721PheAla: 2.721 ± 0.476
0.614PheCys: 0.614 ± 0.22
2.546PheAsp: 2.546 ± 0.416
2.019PheGlu: 2.019 ± 0.346
1.141PhePhe: 1.141 ± 0.277
2.546PheGly: 2.546 ± 0.372
1.229PheHis: 1.229 ± 0.342
2.019PheIle: 2.019 ± 0.511
2.633PheLys: 2.633 ± 0.588
2.809PheLeu: 2.809 ± 0.548
1.141PheMet: 1.141 ± 0.325
1.931PheAsn: 1.931 ± 0.342
2.019PhePro: 2.019 ± 0.465
1.141PheGln: 1.141 ± 0.319
2.195PheArg: 2.195 ± 0.361
1.931PheSer: 1.931 ± 0.378
3.423PheThr: 3.423 ± 0.478
2.546PheVal: 2.546 ± 0.47
0.527PheTrp: 0.527 ± 0.141
1.317PheTyr: 1.317 ± 0.248
0.0PheXaa: 0.0 ± 0.0
Gly
7.374GlyAla: 7.374 ± 0.92
0.878GlyCys: 0.878 ± 0.327
5.355GlyAsp: 5.355 ± 0.851
4.038GlyGlu: 4.038 ± 0.422
2.985GlyPhe: 2.985 ± 0.476
4.828GlyGly: 4.828 ± 0.687
0.79GlyHis: 0.79 ± 0.216
3.775GlyIle: 3.775 ± 0.519
4.74GlyLys: 4.74 ± 0.778
6.584GlyLeu: 6.584 ± 0.829
1.931GlyMet: 1.931 ± 0.393
4.301GlyAsn: 4.301 ± 0.69
0.614GlyPro: 0.614 ± 0.249
2.721GlyGln: 2.721 ± 0.561
3.95GlyArg: 3.95 ± 0.573
5.267GlySer: 5.267 ± 0.647
4.74GlyThr: 4.74 ± 0.594
4.477GlyVal: 4.477 ± 0.632
1.931GlyTrp: 1.931 ± 0.385
3.687GlyTyr: 3.687 ± 0.645
0.0GlyXaa: 0.0 ± 0.0
His
1.141HisAla: 1.141 ± 0.301
0.263HisCys: 0.263 ± 0.15
0.878HisAsp: 0.878 ± 0.265
0.878HisGlu: 0.878 ± 0.246
0.966HisPhe: 0.966 ± 0.32
1.141HisGly: 1.141 ± 0.307
0.351HisHis: 0.351 ± 0.165
1.58HisIle: 1.58 ± 0.304
1.317HisLys: 1.317 ± 0.412
2.019HisLeu: 2.019 ± 0.433
0.878HisMet: 0.878 ± 0.227
0.966HisAsn: 0.966 ± 0.236
0.439HisPro: 0.439 ± 0.159
0.527HisGln: 0.527 ± 0.159
0.79HisArg: 0.79 ± 0.306
1.404HisSer: 1.404 ± 0.324
1.053HisThr: 1.053 ± 0.301
1.756HisVal: 1.756 ± 0.341
0.614HisTrp: 0.614 ± 0.24
0.702HisTyr: 0.702 ± 0.222
0.0HisXaa: 0.0 ± 0.0
Ile
4.213IleAla: 4.213 ± 0.603
0.702IleCys: 0.702 ± 0.236
2.633IleAsp: 2.633 ± 0.439
3.511IleGlu: 3.511 ± 0.46
0.966IlePhe: 0.966 ± 0.222
3.599IleGly: 3.599 ± 0.484
1.668IleHis: 1.668 ± 0.47
1.756IleIle: 1.756 ± 0.36
3.336IleLys: 3.336 ± 0.532
4.389IleLeu: 4.389 ± 0.69
2.195IleMet: 2.195 ± 0.479
2.107IleAsn: 2.107 ± 0.426
2.809IlePro: 2.809 ± 0.462
2.195IleGln: 2.195 ± 0.486
3.072IleArg: 3.072 ± 0.499
2.107IleSer: 2.107 ± 0.399
2.633IleThr: 2.633 ± 0.629
3.16IleVal: 3.16 ± 0.676
0.614IleTrp: 0.614 ± 0.229
2.107IleTyr: 2.107 ± 0.458
0.0IleXaa: 0.0 ± 0.0
Lys
7.549LysAla: 7.549 ± 0.93
0.527LysCys: 0.527 ± 0.196
3.423LysAsp: 3.423 ± 0.501
5.179LysGlu: 5.179 ± 0.812
2.721LysPhe: 2.721 ± 0.442
5.091LysGly: 5.091 ± 0.726
1.843LysHis: 1.843 ± 0.444
2.458LysIle: 2.458 ± 0.414
3.336LysLys: 3.336 ± 0.593
5.442LysLeu: 5.442 ± 0.585
1.404LysMet: 1.404 ± 0.318
2.546LysAsn: 2.546 ± 0.571
2.633LysPro: 2.633 ± 0.493
3.16LysGln: 3.16 ± 0.377
3.072LysArg: 3.072 ± 0.624
3.599LysSer: 3.599 ± 0.493
3.16LysThr: 3.16 ± 0.469
5.091LysVal: 5.091 ± 0.797
0.966LysTrp: 0.966 ± 0.267
2.107LysTyr: 2.107 ± 0.36
0.0LysXaa: 0.0 ± 0.0
Leu
6.759LeuAla: 6.759 ± 1.012
0.79LeuCys: 0.79 ± 0.27
4.565LeuAsp: 4.565 ± 0.632
5.969LeuGlu: 5.969 ± 0.812
2.195LeuPhe: 2.195 ± 0.422
4.652LeuGly: 4.652 ± 0.683
1.229LeuHis: 1.229 ± 0.34
4.038LeuIle: 4.038 ± 0.658
6.671LeuLys: 6.671 ± 0.696
4.828LeuLeu: 4.828 ± 0.71
2.633LeuMet: 2.633 ± 0.522
4.301LeuAsn: 4.301 ± 0.536
2.809LeuPro: 2.809 ± 0.626
3.423LeuGln: 3.423 ± 0.535
6.057LeuArg: 6.057 ± 0.52
5.004LeuSer: 5.004 ± 0.435
3.336LeuThr: 3.336 ± 0.527
5.179LeuVal: 5.179 ± 0.574
1.404LeuTrp: 1.404 ± 0.381
2.37LeuTyr: 2.37 ± 0.419
0.0LeuXaa: 0.0 ± 0.0
Met
3.599MetAla: 3.599 ± 0.531
0.176MetCys: 0.176 ± 0.111
1.053MetAsp: 1.053 ± 0.445
2.019MetGlu: 2.019 ± 0.433
1.141MetPhe: 1.141 ± 0.297
1.756MetGly: 1.756 ± 0.318
0.614MetHis: 0.614 ± 0.23
1.053MetIle: 1.053 ± 0.384
1.58MetLys: 1.58 ± 0.303
2.546MetLeu: 2.546 ± 0.379
0.527MetMet: 0.527 ± 0.162
2.107MetAsn: 2.107 ± 0.451
0.966MetPro: 0.966 ± 0.34
1.317MetGln: 1.317 ± 0.414
1.756MetArg: 1.756 ± 0.354
1.668MetSer: 1.668 ± 0.396
2.019MetThr: 2.019 ± 0.406
1.843MetVal: 1.843 ± 0.347
0.263MetTrp: 0.263 ± 0.123
0.614MetTyr: 0.614 ± 0.184
0.0MetXaa: 0.0 ± 0.0
Asn
2.721AsnAla: 2.721 ± 0.347
0.527AsnCys: 0.527 ± 0.231
3.775AsnAsp: 3.775 ± 0.557
2.633AsnGlu: 2.633 ± 0.432
2.019AsnPhe: 2.019 ± 0.393
4.828AsnGly: 4.828 ± 0.532
1.053AsnHis: 1.053 ± 0.265
2.458AsnIle: 2.458 ± 0.517
2.633AsnLys: 2.633 ± 0.403
3.511AsnLeu: 3.511 ± 0.578
1.053AsnMet: 1.053 ± 0.329
1.668AsnAsn: 1.668 ± 0.414
3.336AsnPro: 3.336 ± 0.477
1.756AsnGln: 1.756 ± 0.447
2.721AsnArg: 2.721 ± 0.475
2.195AsnSer: 2.195 ± 0.431
3.16AsnThr: 3.16 ± 0.511
3.862AsnVal: 3.862 ± 0.486
0.79AsnTrp: 0.79 ± 0.242
1.668AsnTyr: 1.668 ± 0.459
0.0AsnXaa: 0.0 ± 0.0
Pro
2.458ProAla: 2.458 ± 0.419
0.351ProCys: 0.351 ± 0.153
2.282ProAsp: 2.282 ± 0.336
3.599ProGlu: 3.599 ± 0.528
1.756ProPhe: 1.756 ± 0.362
0.0ProGly: 0.0 ± 0.0
0.527ProHis: 0.527 ± 0.202
1.492ProIle: 1.492 ± 0.436
2.897ProLys: 2.897 ± 0.658
2.195ProLeu: 2.195 ± 0.43
0.966ProMet: 0.966 ± 0.364
2.458ProAsn: 2.458 ± 0.528
1.141ProPro: 1.141 ± 0.282
2.107ProGln: 2.107 ± 0.493
1.843ProArg: 1.843 ± 0.316
2.195ProSer: 2.195 ± 0.475
3.072ProThr: 3.072 ± 0.462
2.721ProVal: 2.721 ± 0.34
0.614ProTrp: 0.614 ± 0.165
1.756ProTyr: 1.756 ± 0.45
0.0ProXaa: 0.0 ± 0.0
Gln
4.389GlnAla: 4.389 ± 0.696
0.263GlnCys: 0.263 ± 0.159
2.721GlnAsp: 2.721 ± 0.645
2.37GlnGlu: 2.37 ± 0.517
2.019GlnPhe: 2.019 ± 0.322
3.16GlnGly: 3.16 ± 0.461
0.702GlnHis: 0.702 ± 0.197
1.931GlnIle: 1.931 ± 0.467
2.282GlnLys: 2.282 ± 0.638
3.687GlnLeu: 3.687 ± 0.473
0.966GlnMet: 0.966 ± 0.33
1.404GlnAsn: 1.404 ± 0.37
1.843GlnPro: 1.843 ± 0.35
2.458GlnGln: 2.458 ± 0.497
2.019GlnArg: 2.019 ± 0.514
2.546GlnSer: 2.546 ± 0.49
1.843GlnThr: 1.843 ± 0.291
2.282GlnVal: 2.282 ± 0.459
1.053GlnTrp: 1.053 ± 0.313
1.492GlnTyr: 1.492 ± 0.496
0.0GlnXaa: 0.0 ± 0.0
Arg
4.038ArgAla: 4.038 ± 0.513
0.878ArgCys: 0.878 ± 0.311
4.213ArgAsp: 4.213 ± 0.662
4.038ArgGlu: 4.038 ± 0.568
2.019ArgPhe: 2.019 ± 0.429
4.301ArgGly: 4.301 ± 0.582
0.878ArgHis: 0.878 ± 0.3
2.897ArgIle: 2.897 ± 0.451
3.687ArgLys: 3.687 ± 0.67
5.442ArgLeu: 5.442 ± 0.541
2.107ArgMet: 2.107 ± 0.466
1.843ArgAsn: 1.843 ± 0.312
1.317ArgPro: 1.317 ± 0.374
1.931ArgGln: 1.931 ± 0.36
2.195ArgArg: 2.195 ± 0.541
2.985ArgSer: 2.985 ± 0.698
2.633ArgThr: 2.633 ± 0.398
4.565ArgVal: 4.565 ± 0.694
0.527ArgTrp: 0.527 ± 0.243
1.931ArgTyr: 1.931 ± 0.491
0.0ArgXaa: 0.0 ± 0.0
Ser
4.74SerAla: 4.74 ± 0.448
0.702SerCys: 0.702 ± 0.238
3.423SerAsp: 3.423 ± 0.636
2.37SerGlu: 2.37 ± 0.321
2.985SerPhe: 2.985 ± 0.426
5.267SerGly: 5.267 ± 0.556
1.317SerHis: 1.317 ± 0.447
3.336SerIle: 3.336 ± 0.58
3.95SerLys: 3.95 ± 0.53
3.511SerLeu: 3.511 ± 0.515
1.58SerMet: 1.58 ± 0.36
2.809SerAsn: 2.809 ± 0.441
2.195SerPro: 2.195 ± 0.461
2.985SerGln: 2.985 ± 0.616
3.16SerArg: 3.16 ± 0.474
4.301SerSer: 4.301 ± 0.562
3.599SerThr: 3.599 ± 0.571
5.267SerVal: 5.267 ± 0.663
1.141SerTrp: 1.141 ± 0.305
2.195SerTyr: 2.195 ± 0.514
0.0SerXaa: 0.0 ± 0.0
Thr
4.389ThrAla: 4.389 ± 0.792
0.614ThrCys: 0.614 ± 0.243
2.809ThrAsp: 2.809 ± 0.537
3.16ThrGlu: 3.16 ± 0.565
2.282ThrPhe: 2.282 ± 0.469
4.74ThrGly: 4.74 ± 0.403
1.141ThrHis: 1.141 ± 0.305
3.95ThrIle: 3.95 ± 0.669
5.091ThrLys: 5.091 ± 0.566
4.828ThrLeu: 4.828 ± 0.491
0.966ThrMet: 0.966 ± 0.324
2.546ThrAsn: 2.546 ± 0.451
2.019ThrPro: 2.019 ± 0.32
2.721ThrGln: 2.721 ± 0.468
2.37ThrArg: 2.37 ± 0.538
3.423ThrSer: 3.423 ± 0.708
3.599ThrThr: 3.599 ± 0.751
3.599ThrVal: 3.599 ± 0.629
0.79ThrTrp: 0.79 ± 0.261
1.756ThrTyr: 1.756 ± 0.419
0.0ThrXaa: 0.0 ± 0.0
Val
5.004ValAla: 5.004 ± 0.594
0.263ValCys: 0.263 ± 0.138
4.301ValAsp: 4.301 ± 0.66
4.565ValGlu: 4.565 ± 0.674
2.458ValPhe: 2.458 ± 0.643
4.389ValGly: 4.389 ± 0.805
1.317ValHis: 1.317 ± 0.35
3.95ValIle: 3.95 ± 0.624
4.74ValLys: 4.74 ± 0.776
5.355ValLeu: 5.355 ± 0.826
2.107ValMet: 2.107 ± 0.434
5.267ValAsn: 5.267 ± 0.595
2.897ValPro: 2.897 ± 0.573
2.458ValGln: 2.458 ± 0.35
3.599ValArg: 3.599 ± 0.638
5.004ValSer: 5.004 ± 0.71
4.652ValThr: 4.652 ± 0.699
5.794ValVal: 5.794 ± 0.865
0.878ValTrp: 0.878 ± 0.271
1.931ValTyr: 1.931 ± 0.476
0.0ValXaa: 0.0 ± 0.0
Trp
1.229TrpAla: 1.229 ± 0.313
0.176TrpCys: 0.176 ± 0.134
0.702TrpAsp: 0.702 ± 0.26
0.966TrpGlu: 0.966 ± 0.349
0.176TrpPhe: 0.176 ± 0.117
1.317TrpGly: 1.317 ± 0.256
0.439TrpHis: 0.439 ± 0.253
0.527TrpIle: 0.527 ± 0.202
1.053TrpLys: 1.053 ± 0.307
1.843TrpLeu: 1.843 ± 0.442
0.614TrpMet: 0.614 ± 0.274
0.702TrpAsn: 0.702 ± 0.227
0.263TrpPro: 0.263 ± 0.158
0.702TrpGln: 0.702 ± 0.25
1.141TrpArg: 1.141 ± 0.291
0.966TrpSer: 0.966 ± 0.281
0.878TrpThr: 0.878 ± 0.291
1.404TrpVal: 1.404 ± 0.484
0.351TrpTrp: 0.351 ± 0.165
0.088TrpTyr: 0.088 ± 0.096
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.721TyrAla: 2.721 ± 0.662
0.176TyrCys: 0.176 ± 0.12
3.248TyrAsp: 3.248 ± 0.649
2.809TyrGlu: 2.809 ± 0.374
0.878TyrPhe: 0.878 ± 0.25
3.95TyrGly: 3.95 ± 0.489
1.053TyrHis: 1.053 ± 0.298
1.931TyrIle: 1.931 ± 0.443
2.019TyrLys: 2.019 ± 0.476
3.072TyrLeu: 3.072 ± 0.68
1.404TyrMet: 1.404 ± 0.272
1.404TyrAsn: 1.404 ± 0.374
1.053TyrPro: 1.053 ± 0.315
1.58TyrGln: 1.58 ± 0.427
1.58TyrArg: 1.58 ± 0.339
1.404TyrSer: 1.404 ± 0.426
1.58TyrThr: 1.58 ± 0.364
2.985TyrVal: 2.985 ± 0.536
0.351TyrTrp: 0.351 ± 0.19
0.966TyrTyr: 0.966 ± 0.294
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 38 proteins (11393 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski