CRESS virus sp. ct4af14
Average proteome isoelectric point is 8.24
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5Q2W895|A0A5Q2W895_9VIRU Capsid protein OS=CRESS virus sp. ct4af14 OX=2656675 PE=4 SV=1
MM1 pKa = 7.26 SRR3 pKa = 11.84 LRR5 pKa = 11.84 RR6 pKa = 11.84 VRR8 pKa = 11.84 FVIKK12 pKa = 9.77 CACCDD17 pKa = 4.01 DD18 pKa = 4.11 LHH20 pKa = 6.62 VASDD24 pKa = 4.09 NCPKK28 pKa = 10.57 LPSDD32 pKa = 3.45 VSEE35 pKa = 4.44 DD36 pKa = 3.69 VVVQAAAGEE45 pKa = 4.24 RR46 pKa = 11.84 EE47 pKa = 4.09 ARR49 pKa = 11.84 CRR51 pKa = 11.84 GGGAAEE57 pKa = 4.66 GSAPEE62 pKa = 4.21 GARR65 pKa = 11.84 KK66 pKa = 8.73 EE67 pKa = 4.24 VRR69 pKa = 11.84 NKK71 pKa = 10.18 RR72 pKa = 11.84 ATVHH76 pKa = 5.71 PRR78 pKa = 11.84 EE79 pKa = 4.14 AHH81 pKa = 4.81 EE82 pKa = 3.62 WAARR86 pKa = 11.84 DD87 pKa = 4.49 RR88 pKa = 11.84 ADD90 pKa = 4.09 PDD92 pKa = 3.31 QAA94 pKa = 4.66
Molecular weight: 10.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.819
IPC2_protein 6.825
IPC_protein 7.015
Toseland 6.678
ProMoST 7.468
Dawson 7.614
Bjellqvist 7.673
Wikipedia 7.527
Rodwell 7.6
Grimsley 6.707
Solomon 7.717
Lehninger 7.746
Nozaki 8.141
DTASelect 7.761
Thurlkill 7.805
EMBOSS 7.878
Sillero 8.126
Patrickios 4.711
IPC_peptide 7.717
IPC2_peptide 7.585
IPC2.peptide.svr19 7.382
Protein with the highest isoelectric point:
>tr|A0A5Q2W8W8|A0A5Q2W8W8_9VIRU Uncharacterized protein OS=CRESS virus sp. ct4af14 OX=2656675 PE=4 SV=1
MM1 pKa = 7.06 QPWSQHH7 pKa = 4.44 CTLHH11 pKa = 6.19 YY12 pKa = 9.54 VTLTTARR19 pKa = 11.84 VTSGRR24 pKa = 11.84 RR25 pKa = 11.84 TRR27 pKa = 11.84 PRR29 pKa = 11.84 TQSRR33 pKa = 11.84 YY34 pKa = 8.2 GCSVRR39 pKa = 11.84 PGPLPHH45 pKa = 6.8 HH46 pKa = 6.93 SGRR49 pKa = 11.84 GTPCRR54 pKa = 11.84 STDD57 pKa = 3.16 QQ58 pKa = 3.44
Molecular weight: 6.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.154
IPC2_protein 10.116
IPC_protein 11.403
Toseland 11.462
ProMoST 11.959
Dawson 11.477
Bjellqvist 11.477
Wikipedia 11.959
Rodwell 11.008
Grimsley 11.52
Solomon 11.959
Lehninger 11.871
Nozaki 11.477
DTASelect 11.477
Thurlkill 11.477
EMBOSS 11.959
Sillero 11.477
Patrickios 10.833
IPC_peptide 11.974
IPC2_peptide 10.979
IPC2.peptide.svr19 9.467
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
407
58
255
135.7
14.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.794 ± 3.005
2.457 ± 1.528
5.16 ± 1.35
4.423 ± 1.68
1.966 ± 0.756
7.371 ± 0.434
2.948 ± 1.193
3.44 ± 1.545
4.423 ± 1.321
4.423 ± 0.448
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.211 ± 0.496
1.72 ± 0.481
6.388 ± 1.107
4.914 ± 1.031
9.091 ± 2.976
8.108 ± 1.053
6.143 ± 2.826
7.862 ± 0.852
1.474 ± 0.149
3.686 ± 1.412
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here