Salmonella phage FSL SP-076
Average proteome isoelectric point is 6.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 82 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S4TU53|S4TU53_9CAUD Uncharacterized protein OS=Salmonella phage FSL SP-076 OX=1173762 GN=SP076_00130 PE=4 SV=1
MM1 pKa = 7.54 GLYY4 pKa = 9.98 LYY6 pKa = 9.58 CQNTEE11 pKa = 3.73 CGQYY15 pKa = 10.35 VDD17 pKa = 6.28 GMDD20 pKa = 4.26 CQCCGWTQPLPEE32 pKa = 5.07 PEE34 pKa = 4.37 FEE36 pKa = 4.67 LPEE39 pKa = 4.43 DD40 pKa = 4.3 LKK42 pKa = 11.25 QALQDD47 pKa = 3.55 ALDD50 pKa = 4.55 FVGHH54 pKa = 6.58 DD55 pKa = 4.03 CRR57 pKa = 11.84 FDD59 pKa = 3.18 HH60 pKa = 7.32 HH61 pKa = 7.08 GYY63 pKa = 9.16 CQEE66 pKa = 4.65 HH67 pKa = 6.51 GLQEE71 pKa = 4.21 EE72 pKa = 4.67 DD73 pKa = 3.79 SCFAGVFRR81 pKa = 11.84 KK82 pKa = 10.21 YY83 pKa = 10.96 LEE85 pKa = 4.01
Molecular weight: 9.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.115
IPC2_protein 4.19
IPC_protein 4.101
Toseland 3.923
ProMoST 4.177
Dawson 4.062
Bjellqvist 4.279
Wikipedia 3.973
Rodwell 3.935
Grimsley 3.834
Solomon 4.05
Lehninger 4.012
Nozaki 4.19
DTASelect 4.355
Thurlkill 3.961
EMBOSS 3.986
Sillero 4.215
Patrickios 0.057
IPC_peptide 4.062
IPC2_peptide 4.202
IPC2.peptide.svr19 4.167
Protein with the highest isoelectric point:
>tr|S4TT92|S4TT92_9CAUD Uncharacterized protein OS=Salmonella phage FSL SP-076 OX=1173762 GN=SP076_00395 PE=4 SV=1
MM1 pKa = 7.52 SIKK4 pKa = 9.83 LTVVAKK10 pKa = 10.23 RR11 pKa = 11.84 YY12 pKa = 9.39 NSEE15 pKa = 3.59 KK16 pKa = 10.29 FIRR19 pKa = 11.84 ILMPWGSNISYY30 pKa = 9.6 GRR32 pKa = 11.84 RR33 pKa = 11.84 WQVSFKK39 pKa = 10.5 DD40 pKa = 3.56 GRR42 pKa = 11.84 GRR44 pKa = 11.84 HH45 pKa = 5.31 YY46 pKa = 11.18 DD47 pKa = 3.06 HH48 pKa = 7.2 SFVAKK53 pKa = 7.73 PTRR56 pKa = 11.84 KK57 pKa = 9.61 QIRR60 pKa = 11.84 KK61 pKa = 9.18 LIKK64 pKa = 10.3 DD65 pKa = 3.3 INKK68 pKa = 9.9
Molecular weight: 8.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.269
IPC2_protein 10.175
IPC_protein 11.082
Toseland 11.272
ProMoST 11.272
Dawson 11.316
Bjellqvist 11.096
Wikipedia 11.594
Rodwell 11.564
Grimsley 11.345
Solomon 11.564
Lehninger 11.506
Nozaki 11.242
DTASelect 11.096
Thurlkill 11.257
EMBOSS 11.696
Sillero 11.257
Patrickios 11.301
IPC_peptide 11.564
IPC2_peptide 10.16
IPC2.peptide.svr19 8.306
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
82
0
82
21057
53
3595
256.8
28.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.301 ± 0.349
0.907 ± 0.167
6.169 ± 0.185
6.444 ± 0.281
3.671 ± 0.168
6.782 ± 0.338
1.615 ± 0.142
6.473 ± 0.239
6.254 ± 0.309
8.577 ± 0.217
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.712 ± 0.149
5.547 ± 0.245
4.022 ± 0.146
4.678 ± 0.314
4.127 ± 0.187
6.178 ± 0.194
6.136 ± 0.294
6.839 ± 0.24
1.035 ± 0.1
3.533 ± 0.223
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here