Meyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324) (Yeast) (Candida guilliermondii)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Debaryomycetaceae; Meyerozyma; Meyerozyma guilliermondii

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5919 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A5DI00|A5DI00_PICGU Uncharacterized protein OS=Meyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324) OX=294746 GN=PGUG_02901 PE=4 SV=2
MM1 pKa = 7.76KK2 pKa = 9.26ITTIFHH8 pKa = 5.87TVLLASAAVNAQDD21 pKa = 3.59APKK24 pKa = 10.51KK25 pKa = 10.44RR26 pKa = 11.84GFLSDD31 pKa = 4.06LFGGSDD37 pKa = 3.33AAAPAAAPAAASAATSSTTAAAAAAPVAAATSSAAGVLDD76 pKa = 4.85LFGLDD81 pKa = 3.63SSNSNSTSSSSSSSSSSSSSSSSSSSSSSSSSTKK115 pKa = 10.3APAFRR120 pKa = 11.84VLAAQGTTQSSSSAPATTDD139 pKa = 2.87SGSLFDD145 pKa = 5.19FLNVDD150 pKa = 4.02SSASSSSSSPASSSSKK166 pKa = 10.2VSSSSQTGGLLNQLFAVAPSGSSGSSGSSSASASASKK203 pKa = 10.5SASASSASASVTAAPLGFLGDD224 pKa = 4.04LFGGSSSSKK233 pKa = 9.91ASSGSVSGSSSASSADD249 pKa = 4.36DD250 pKa = 4.07EE251 pKa = 4.67EE252 pKa = 6.7CDD254 pKa = 5.43DD255 pKa = 4.39EE256 pKa = 4.67TDD258 pKa = 3.89SASPTDD264 pKa = 4.45DD265 pKa = 3.56PVSVPPAFSSMSLPPLTGSPSGSLTSLAAVSGQPTSGASRR305 pKa = 11.84SSGVSSARR313 pKa = 11.84SLAASARR320 pKa = 11.84SSVSASSEE328 pKa = 3.86EE329 pKa = 4.09DD330 pKa = 4.3DD331 pKa = 4.32EE332 pKa = 5.56DD333 pKa = 5.89CEE335 pKa = 4.73TEE337 pKa = 4.16SSLPTLPPLPSLSGSGGVLPTLSGSGGILPTLSGSGGILPTLGSASRR384 pKa = 11.84NSTGSTSATVSRR396 pKa = 11.84STASDD401 pKa = 3.64DD402 pKa = 5.68GDD404 pKa = 6.12DD405 pKa = 4.14EE406 pKa = 6.26DD407 pKa = 5.75CDD409 pKa = 4.05PSEE412 pKa = 4.24TTIKK416 pKa = 10.76GDD418 pKa = 3.29PTYY421 pKa = 10.56ATLPTEE427 pKa = 4.03ITSNGDD433 pKa = 3.0PTGITTRR440 pKa = 11.84GTATTTVITTVKK452 pKa = 6.89TTNGGSAVTYY462 pKa = 6.96TTTKK466 pKa = 10.1VVQPTQGSEE475 pKa = 3.85DD476 pKa = 4.35DD477 pKa = 4.16EE478 pKa = 5.35DD479 pKa = 4.88CEE481 pKa = 4.37EE482 pKa = 5.48YY483 pKa = 9.62YY484 pKa = 9.96TQTQTYY490 pKa = 6.87YY491 pKa = 7.96TTYY494 pKa = 7.66TTVINGATVTKK505 pKa = 10.51VVEE508 pKa = 4.12CDD510 pKa = 3.03EE511 pKa = 4.57CKK513 pKa = 10.35GTGSGSGSSGSGSSGSGSSGSGSYY537 pKa = 10.82GGGSSSGSGVDD548 pKa = 3.98EE549 pKa = 4.72DD550 pKa = 5.64CEE552 pKa = 4.68DD553 pKa = 4.62DD554 pKa = 4.19EE555 pKa = 5.6SSPSYY560 pKa = 11.14GSGSGSGTSYY570 pKa = 11.44GSGSGSSGSGSNYY583 pKa = 9.89GSGSGSSSGSGSGSGSTSKK602 pKa = 10.38PGYY605 pKa = 9.74LIPSDD610 pKa = 4.08DD611 pKa = 4.06QCDD614 pKa = 3.92EE615 pKa = 4.4DD616 pKa = 6.2CVVCDD621 pKa = 5.59DD622 pKa = 5.63SDD624 pKa = 6.34DD625 pKa = 4.87EE626 pKa = 4.96DD627 pKa = 5.29FDD629 pKa = 4.07WFGNDD634 pKa = 4.16TDD636 pKa = 4.9DD637 pKa = 5.39EE638 pKa = 5.37CDD640 pKa = 3.85ADD642 pKa = 5.54CDD644 pKa = 3.77EE645 pKa = 5.33CDD647 pKa = 3.68AGTSGSTSGSTSGSGSGSGSGSGSGSGSKK676 pKa = 10.14SGSGSGSGSGGSGSGSGSGGSGGAKK701 pKa = 9.26TYY703 pKa = 10.84VSTVRR708 pKa = 11.84TTAAGEE714 pKa = 4.37STWYY718 pKa = 8.1STSVSTSTHH727 pKa = 6.05SPDD730 pKa = 5.14DD731 pKa = 3.66EE732 pKa = 6.58CDD734 pKa = 3.76DD735 pKa = 4.42EE736 pKa = 5.62EE737 pKa = 5.18SATTTKK743 pKa = 10.73SATTTSGYY751 pKa = 11.39ANSTSKK757 pKa = 8.68QTGGFLADD765 pKa = 3.74LFGRR769 pKa = 11.84DD770 pKa = 3.72VEE772 pKa = 4.48ISSSAWANTSVTSAPVVEE790 pKa = 4.16QHH792 pKa = 6.8PSNGSRR798 pKa = 11.84QTSFSTALVALVVSIVAFTLII819 pKa = 4.01

Molecular weight: 78.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A5DQM9|A5DQM9_PICGU TAFII28 domain-containing protein OS=Meyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324) OX=294746 GN=PGUG_05580 PE=3 SV=2
MM1 pKa = 7.0YY2 pKa = 10.63VHH4 pKa = 7.36AKK6 pKa = 9.26TDD8 pKa = 3.27GEE10 pKa = 4.28MQKK13 pKa = 10.97KK14 pKa = 9.91KK15 pKa = 9.33MRR17 pKa = 11.84LWEE20 pKa = 3.69LHH22 pKa = 5.22GAINAGLTPILTSPYY37 pKa = 7.88GTKK40 pKa = 10.34ASEE43 pKa = 3.66ITRR46 pKa = 11.84FRR48 pKa = 11.84WCVNPPSNSILVGRR62 pKa = 11.84CMRR65 pKa = 11.84LICPDD70 pKa = 3.05HH71 pKa = 6.01SHH73 pKa = 6.72AVRR76 pKa = 11.84SCRR79 pKa = 11.84PSSTRR84 pKa = 11.84ARR86 pKa = 11.84ATTTARR92 pKa = 11.84DD93 pKa = 3.54AHH95 pKa = 6.57HH96 pKa = 6.64ATAGTRR102 pKa = 11.84RR103 pKa = 11.84WCPSILMDD111 pKa = 4.84LGVWVGKK118 pKa = 10.2RR119 pKa = 11.84LAGFVGVWACGVGPPTLLTNRR140 pKa = 11.84EE141 pKa = 4.03RR142 pKa = 11.84QVSIMTDD149 pKa = 3.2TAAYY153 pKa = 7.42EE154 pKa = 4.02YY155 pKa = 10.15PAIRR159 pKa = 11.84KK160 pKa = 8.49SFQRR164 pKa = 11.84FQIMFACRR172 pKa = 11.84ILIQHH177 pKa = 6.57RR178 pKa = 11.84RR179 pKa = 11.84AII181 pKa = 3.94

Molecular weight: 20.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5919

0

5919

2761741

34

4897

466.6

52.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.352 ± 0.029

1.224 ± 0.011

5.751 ± 0.024

6.451 ± 0.029

4.421 ± 0.022

5.503 ± 0.035

2.318 ± 0.015

5.959 ± 0.021

6.508 ± 0.03

9.256 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.098 ± 0.013

4.98 ± 0.019

4.705 ± 0.027

3.924 ± 0.022

4.717 ± 0.019

9.27 ± 0.044

5.713 ± 0.033

6.334 ± 0.026

1.114 ± 0.01

3.401 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski