Amino acid dipepetide frequency for Edwardsiella phage PEi21

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.713AlaAla: 12.713 ± 1.588
1.249AlaCys: 1.249 ± 0.274
5.732AlaAsp: 5.732 ± 0.822
5.438AlaGlu: 5.438 ± 0.72
2.866AlaPhe: 2.866 ± 0.429
7.422AlaGly: 7.422 ± 1.003
1.249AlaHis: 1.249 ± 0.24
4.703AlaIle: 4.703 ± 0.61
3.895AlaLys: 3.895 ± 0.583
6.761AlaLeu: 6.761 ± 0.57
3.38AlaMet: 3.38 ± 0.555
4.483AlaAsn: 4.483 ± 0.714
4.409AlaPro: 4.409 ± 1.059
3.748AlaGln: 3.748 ± 0.514
5.218AlaArg: 5.218 ± 0.664
5.805AlaSer: 5.805 ± 0.595
6.246AlaThr: 6.246 ± 0.774
6.099AlaVal: 6.099 ± 0.725
1.543AlaTrp: 1.543 ± 0.343
2.939AlaTyr: 2.939 ± 0.427
0.0AlaXaa: 0.0 ± 0.0
Cys
1.176CysAla: 1.176 ± 0.287
0.073CysCys: 0.073 ± 0.077
1.029CysAsp: 1.029 ± 0.335
1.029CysGlu: 1.029 ± 0.31
0.367CysPhe: 0.367 ± 0.142
1.764CysGly: 1.764 ± 0.424
0.514CysHis: 0.514 ± 0.169
0.588CysIle: 0.588 ± 0.206
0.882CysLys: 0.882 ± 0.214
0.441CysLeu: 0.441 ± 0.175
0.367CysMet: 0.367 ± 0.147
0.808CysAsn: 0.808 ± 0.223
1.029CysPro: 1.029 ± 0.359
0.955CysGln: 0.955 ± 0.219
1.323CysArg: 1.323 ± 0.328
0.808CysSer: 0.808 ± 0.233
0.514CysThr: 0.514 ± 0.176
1.176CysVal: 1.176 ± 0.299
0.073CysTrp: 0.073 ± 0.076
0.735CysTyr: 0.735 ± 0.186
0.0CysXaa: 0.0 ± 0.0
Asp
7.569AspAla: 7.569 ± 0.779
1.029AspCys: 1.029 ± 0.245
3.527AspAsp: 3.527 ± 0.687
3.895AspGlu: 3.895 ± 0.507
2.499AspPhe: 2.499 ± 0.476
6.246AspGly: 6.246 ± 0.53
1.396AspHis: 1.396 ± 0.254
3.674AspIle: 3.674 ± 0.508
3.307AspLys: 3.307 ± 0.464
4.703AspLeu: 4.703 ± 0.769
1.102AspMet: 1.102 ± 0.37
2.425AspAsn: 2.425 ± 0.396
2.866AspPro: 2.866 ± 0.394
1.323AspGln: 1.323 ± 0.337
3.16AspArg: 3.16 ± 0.463
2.792AspSer: 2.792 ± 0.54
2.792AspThr: 2.792 ± 0.383
5.658AspVal: 5.658 ± 0.637
1.837AspTrp: 1.837 ± 0.364
1.911AspTyr: 1.911 ± 0.292
0.0AspXaa: 0.0 ± 0.0
Glu
4.703GluAla: 4.703 ± 0.66
0.882GluCys: 0.882 ± 0.255
1.837GluAsp: 1.837 ± 0.566
3.968GluGlu: 3.968 ± 0.699
2.499GluPhe: 2.499 ± 0.557
2.866GluGly: 2.866 ± 0.44
1.396GluHis: 1.396 ± 0.258
4.336GluIle: 4.336 ± 0.488
2.572GluLys: 2.572 ± 0.475
5.585GluLeu: 5.585 ± 0.648
1.837GluMet: 1.837 ± 0.394
1.837GluAsn: 1.837 ± 0.315
1.249GluPro: 1.249 ± 0.283
2.866GluGln: 2.866 ± 0.567
3.748GluArg: 3.748 ± 0.44
3.086GluSer: 3.086 ± 0.586
2.278GluThr: 2.278 ± 0.364
3.601GluVal: 3.601 ± 0.731
1.249GluTrp: 1.249 ± 0.251
1.764GluTyr: 1.764 ± 0.381
0.0GluXaa: 0.0 ± 0.0
Phe
2.205PheAla: 2.205 ± 0.458
0.514PheCys: 0.514 ± 0.203
2.499PheAsp: 2.499 ± 0.471
2.499PheGlu: 2.499 ± 0.522
0.808PhePhe: 0.808 ± 0.252
2.646PheGly: 2.646 ± 0.422
0.661PheHis: 0.661 ± 0.224
1.617PheIle: 1.617 ± 0.318
2.205PheLys: 2.205 ± 0.44
1.543PheLeu: 1.543 ± 0.286
1.102PheMet: 1.102 ± 0.266
2.131PheAsn: 2.131 ± 0.428
1.543PhePro: 1.543 ± 0.406
0.661PheGln: 0.661 ± 0.205
1.911PheArg: 1.911 ± 0.382
2.058PheSer: 2.058 ± 0.349
2.866PheThr: 2.866 ± 0.478
2.205PheVal: 2.205 ± 0.413
0.294PheTrp: 0.294 ± 0.116
1.617PheTyr: 1.617 ± 0.345
0.0PheXaa: 0.0 ± 0.0
Gly
7.79GlyAla: 7.79 ± 1.101
1.617GlyCys: 1.617 ± 0.432
5.218GlyAsp: 5.218 ± 0.612
4.483GlyGlu: 4.483 ± 0.546
2.131GlyPhe: 2.131 ± 0.461
6.467GlyGly: 6.467 ± 1.068
1.543GlyHis: 1.543 ± 0.296
3.748GlyIle: 3.748 ± 0.381
6.099GlyLys: 6.099 ± 0.762
5.658GlyLeu: 5.658 ± 0.672
1.984GlyMet: 1.984 ± 0.392
3.601GlyAsn: 3.601 ± 0.567
1.029GlyPro: 1.029 ± 0.222
1.837GlyGln: 1.837 ± 0.323
3.674GlyArg: 3.674 ± 0.531
3.821GlySer: 3.821 ± 0.509
5.218GlyThr: 5.218 ± 0.802
7.496GlyVal: 7.496 ± 0.749
1.837GlyTrp: 1.837 ± 0.298
3.307GlyTyr: 3.307 ± 0.487
0.0GlyXaa: 0.0 ± 0.0
His
1.323HisAla: 1.323 ± 0.343
0.22HisCys: 0.22 ± 0.148
1.176HisAsp: 1.176 ± 0.236
0.441HisGlu: 0.441 ± 0.164
0.955HisPhe: 0.955 ± 0.284
1.47HisGly: 1.47 ± 0.461
0.808HisHis: 0.808 ± 0.273
1.323HisIle: 1.323 ± 0.335
1.029HisLys: 1.029 ± 0.24
2.058HisLeu: 2.058 ± 0.391
0.367HisMet: 0.367 ± 0.164
0.735HisAsn: 0.735 ± 0.193
0.882HisPro: 0.882 ± 0.278
0.367HisGln: 0.367 ± 0.148
1.029HisArg: 1.029 ± 0.267
1.249HisSer: 1.249 ± 0.244
1.249HisThr: 1.249 ± 0.295
1.47HisVal: 1.47 ± 0.335
0.514HisTrp: 0.514 ± 0.165
0.367HisTyr: 0.367 ± 0.148
0.0HisXaa: 0.0 ± 0.0
Ile
4.85IleAla: 4.85 ± 0.618
0.735IleCys: 0.735 ± 0.238
4.63IleAsp: 4.63 ± 0.522
3.086IleGlu: 3.086 ± 0.458
1.543IlePhe: 1.543 ± 0.356
3.307IleGly: 3.307 ± 0.455
1.102IleHis: 1.102 ± 0.312
2.131IleIle: 2.131 ± 0.35
2.792IleLys: 2.792 ± 0.416
2.646IleLeu: 2.646 ± 0.422
1.396IleMet: 1.396 ± 0.349
3.086IleAsn: 3.086 ± 0.452
2.352IlePro: 2.352 ± 0.458
1.837IleGln: 1.837 ± 0.49
3.013IleArg: 3.013 ± 0.508
2.719IleSer: 2.719 ± 0.347
4.997IleThr: 4.997 ± 0.58
4.409IleVal: 4.409 ± 0.554
0.147IleTrp: 0.147 ± 0.099
1.764IleTyr: 1.764 ± 0.385
0.0IleXaa: 0.0 ± 0.0
Lys
4.189LysAla: 4.189 ± 0.571
1.102LysCys: 1.102 ± 0.314
3.013LysAsp: 3.013 ± 0.445
1.47LysGlu: 1.47 ± 0.358
1.323LysPhe: 1.323 ± 0.294
3.527LysGly: 3.527 ± 0.408
1.102LysHis: 1.102 ± 0.241
2.646LysIle: 2.646 ± 0.378
2.572LysLys: 2.572 ± 0.459
4.997LysLeu: 4.997 ± 0.593
1.837LysMet: 1.837 ± 0.354
2.278LysAsn: 2.278 ± 0.399
2.058LysPro: 2.058 ± 0.405
1.543LysGln: 1.543 ± 0.359
3.895LysArg: 3.895 ± 0.723
4.115LysSer: 4.115 ± 0.486
3.454LysThr: 3.454 ± 0.429
2.646LysVal: 2.646 ± 0.542
0.735LysTrp: 0.735 ± 0.212
1.69LysTyr: 1.69 ± 0.503
0.0LysXaa: 0.0 ± 0.0
Leu
5.438LeuAla: 5.438 ± 0.579
1.47LeuCys: 1.47 ± 0.298
6.026LeuAsp: 6.026 ± 0.592
3.968LeuGlu: 3.968 ± 0.565
2.719LeuPhe: 2.719 ± 0.479
4.703LeuGly: 4.703 ± 0.542
0.808LeuHis: 0.808 ± 0.265
3.821LeuIle: 3.821 ± 0.489
3.748LeuLys: 3.748 ± 0.479
5.218LeuLeu: 5.218 ± 0.622
2.719LeuMet: 2.719 ± 0.442
3.307LeuAsn: 3.307 ± 0.511
2.278LeuPro: 2.278 ± 0.372
2.278LeuGln: 2.278 ± 0.345
5.732LeuArg: 5.732 ± 1.193
5.218LeuSer: 5.218 ± 0.641
6.099LeuThr: 6.099 ± 0.604
4.262LeuVal: 4.262 ± 0.457
1.029LeuTrp: 1.029 ± 0.28
2.058LeuTyr: 2.058 ± 0.467
0.0LeuXaa: 0.0 ± 0.0
Met
2.792MetAla: 2.792 ± 0.578
0.441MetCys: 0.441 ± 0.216
1.102MetAsp: 1.102 ± 0.334
0.882MetGlu: 0.882 ± 0.269
0.882MetPhe: 0.882 ± 0.235
2.058MetGly: 2.058 ± 0.463
0.22MetHis: 0.22 ± 0.132
1.911MetIle: 1.911 ± 0.515
1.543MetLys: 1.543 ± 0.325
2.352MetLeu: 2.352 ± 0.442
1.102MetMet: 1.102 ± 0.272
1.69MetAsn: 1.69 ± 0.36
1.029MetPro: 1.029 ± 0.288
1.249MetGln: 1.249 ± 0.307
2.572MetArg: 2.572 ± 0.382
2.131MetSer: 2.131 ± 0.435
2.646MetThr: 2.646 ± 0.386
1.323MetVal: 1.323 ± 0.335
0.22MetTrp: 0.22 ± 0.141
1.323MetTyr: 1.323 ± 0.31
0.0MetXaa: 0.0 ± 0.0
Asn
4.777AsnAla: 4.777 ± 0.626
0.808AsnCys: 0.808 ± 0.242
2.352AsnAsp: 2.352 ± 0.374
1.47AsnGlu: 1.47 ± 0.325
1.837AsnPhe: 1.837 ± 0.377
4.189AsnGly: 4.189 ± 0.667
1.249AsnHis: 1.249 ± 0.339
2.646AsnIle: 2.646 ± 0.387
2.058AsnLys: 2.058 ± 0.364
2.719AsnLeu: 2.719 ± 0.619
1.396AsnMet: 1.396 ± 0.345
2.131AsnAsn: 2.131 ± 0.408
2.719AsnPro: 2.719 ± 0.448
1.69AsnGln: 1.69 ± 0.327
2.205AsnArg: 2.205 ± 0.314
2.205AsnSer: 2.205 ± 0.363
2.719AsnThr: 2.719 ± 0.462
4.042AsnVal: 4.042 ± 0.395
0.735AsnTrp: 0.735 ± 0.205
1.396AsnTyr: 1.396 ± 0.319
0.0AsnXaa: 0.0 ± 0.0
Pro
4.703ProAla: 4.703 ± 0.866
0.955ProCys: 0.955 ± 0.294
2.719ProAsp: 2.719 ± 0.369
2.939ProGlu: 2.939 ± 0.701
1.396ProPhe: 1.396 ± 0.326
3.821ProGly: 3.821 ± 0.523
0.441ProHis: 0.441 ± 0.163
1.69ProIle: 1.69 ± 0.328
1.396ProLys: 1.396 ± 0.35
2.205ProLeu: 2.205 ± 0.421
0.514ProMet: 0.514 ± 0.165
2.352ProAsn: 2.352 ± 0.473
2.425ProPro: 2.425 ± 0.666
1.47ProGln: 1.47 ± 0.31
1.764ProArg: 1.764 ± 0.358
2.572ProSer: 2.572 ± 0.415
2.499ProThr: 2.499 ± 0.496
4.409ProVal: 4.409 ± 1.277
0.588ProTrp: 0.588 ± 0.197
1.176ProTyr: 1.176 ± 0.251
0.0ProXaa: 0.0 ± 0.0
Gln
3.748GlnAla: 3.748 ± 0.553
0.294GlnCys: 0.294 ± 0.158
1.249GlnAsp: 1.249 ± 0.248
1.837GlnGlu: 1.837 ± 0.365
1.249GlnPhe: 1.249 ± 0.336
1.617GlnGly: 1.617 ± 0.293
1.029GlnHis: 1.029 ± 0.209
1.911GlnIle: 1.911 ± 0.412
0.882GlnLys: 0.882 ± 0.254
3.674GlnLeu: 3.674 ± 0.543
1.617GlnMet: 1.617 ± 0.392
1.029GlnAsn: 1.029 ± 0.193
1.102GlnPro: 1.102 ± 0.222
1.764GlnGln: 1.764 ± 0.301
2.205GlnArg: 2.205 ± 0.391
2.058GlnSer: 2.058 ± 0.335
2.425GlnThr: 2.425 ± 0.442
1.764GlnVal: 1.764 ± 0.329
0.588GlnTrp: 0.588 ± 0.189
1.617GlnTyr: 1.617 ± 0.347
0.0GlnXaa: 0.0 ± 0.0
Arg
4.997ArgAla: 4.997 ± 0.647
0.735ArgCys: 0.735 ± 0.224
4.115ArgAsp: 4.115 ± 0.568
3.821ArgGlu: 3.821 ± 0.753
2.205ArgPhe: 2.205 ± 0.501
5.071ArgGly: 5.071 ± 0.89
1.249ArgHis: 1.249 ± 0.37
3.674ArgIle: 3.674 ± 0.544
3.527ArgLys: 3.527 ± 0.481
4.63ArgLeu: 4.63 ± 0.656
1.764ArgMet: 1.764 ± 0.35
3.086ArgAsn: 3.086 ± 0.451
2.278ArgPro: 2.278 ± 0.388
2.205ArgGln: 2.205 ± 0.406
3.16ArgArg: 3.16 ± 0.498
3.748ArgSer: 3.748 ± 0.483
2.499ArgThr: 2.499 ± 0.462
4.336ArgVal: 4.336 ± 0.561
1.47ArgTrp: 1.47 ± 0.329
1.617ArgTyr: 1.617 ± 0.27
0.0ArgXaa: 0.0 ± 0.0
Ser
5.071SerAla: 5.071 ± 0.62
0.808SerCys: 0.808 ± 0.206
4.703SerAsp: 4.703 ± 0.613
2.646SerGlu: 2.646 ± 0.397
1.984SerPhe: 1.984 ± 0.382
4.997SerGly: 4.997 ± 0.642
1.029SerHis: 1.029 ± 0.332
2.792SerIle: 2.792 ± 0.454
2.866SerLys: 2.866 ± 0.422
3.601SerLeu: 3.601 ± 0.481
1.617SerMet: 1.617 ± 0.314
3.674SerAsn: 3.674 ± 0.492
1.911SerPro: 1.911 ± 0.418
2.499SerGln: 2.499 ± 0.402
3.895SerArg: 3.895 ± 0.538
4.042SerSer: 4.042 ± 0.604
3.748SerThr: 3.748 ± 0.5
4.997SerVal: 4.997 ± 0.574
0.661SerTrp: 0.661 ± 0.23
2.058SerTyr: 2.058 ± 0.331
0.0SerXaa: 0.0 ± 0.0
Thr
6.173ThrAla: 6.173 ± 0.716
0.735ThrCys: 0.735 ± 0.189
4.703ThrAsp: 4.703 ± 0.607
3.38ThrGlu: 3.38 ± 0.45
2.425ThrPhe: 2.425 ± 0.349
7.349ThrGly: 7.349 ± 0.729
1.323ThrHis: 1.323 ± 0.284
3.821ThrIle: 3.821 ± 0.44
2.131ThrLys: 2.131 ± 0.337
4.997ThrLeu: 4.997 ± 0.675
1.323ThrMet: 1.323 ± 0.37
1.764ThrAsn: 1.764 ± 0.374
4.189ThrPro: 4.189 ± 0.577
1.396ThrGln: 1.396 ± 0.271
3.895ThrArg: 3.895 ± 0.56
3.527ThrSer: 3.527 ± 0.438
4.042ThrThr: 4.042 ± 0.607
6.32ThrVal: 6.32 ± 0.801
1.396ThrTrp: 1.396 ± 0.281
2.278ThrTyr: 2.278 ± 0.484
0.0ThrXaa: 0.0 ± 0.0
Val
7.496ValAla: 7.496 ± 0.965
1.029ValCys: 1.029 ± 0.299
4.556ValAsp: 4.556 ± 0.525
4.924ValGlu: 4.924 ± 0.532
1.617ValPhe: 1.617 ± 0.294
4.85ValGly: 4.85 ± 0.732
0.808ValHis: 0.808 ± 0.292
3.821ValIle: 3.821 ± 0.501
4.483ValLys: 4.483 ± 0.516
4.483ValLeu: 4.483 ± 0.592
2.866ValMet: 2.866 ± 0.392
2.792ValAsn: 2.792 ± 0.425
4.336ValPro: 4.336 ± 1.003
2.572ValGln: 2.572 ± 0.455
4.189ValArg: 4.189 ± 0.454
4.556ValSer: 4.556 ± 0.544
7.349ValThr: 7.349 ± 0.78
4.703ValVal: 4.703 ± 0.576
1.029ValTrp: 1.029 ± 0.27
2.792ValTyr: 2.792 ± 0.454
0.0ValXaa: 0.0 ± 0.0
Trp
1.323TrpAla: 1.323 ± 0.315
0.514TrpCys: 0.514 ± 0.192
0.808TrpAsp: 0.808 ± 0.251
0.735TrpGlu: 0.735 ± 0.203
1.176TrpPhe: 1.176 ± 0.278
0.735TrpGly: 0.735 ± 0.221
0.735TrpHis: 0.735 ± 0.225
0.588TrpIle: 0.588 ± 0.161
0.808TrpLys: 0.808 ± 0.223
1.323TrpLeu: 1.323 ± 0.252
0.294TrpMet: 0.294 ± 0.138
0.588TrpAsn: 0.588 ± 0.211
0.514TrpPro: 0.514 ± 0.235
0.22TrpGln: 0.22 ± 0.121
1.029TrpArg: 1.029 ± 0.239
1.029TrpSer: 1.029 ± 0.255
0.808TrpThr: 0.808 ± 0.244
1.69TrpVal: 1.69 ± 0.31
0.294TrpTrp: 0.294 ± 0.137
1.176TrpTyr: 1.176 ± 0.266
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.866TyrAla: 2.866 ± 0.444
0.441TyrCys: 0.441 ± 0.199
2.719TyrAsp: 2.719 ± 0.557
1.249TyrGlu: 1.249 ± 0.275
1.102TyrPhe: 1.102 ± 0.274
3.454TyrGly: 3.454 ± 0.397
0.367TyrHis: 0.367 ± 0.146
1.102TyrIle: 1.102 ± 0.277
1.47TyrLys: 1.47 ± 0.299
3.307TyrLeu: 3.307 ± 0.487
0.808TyrMet: 0.808 ± 0.223
1.543TyrAsn: 1.543 ± 0.26
1.837TyrPro: 1.837 ± 0.482
1.176TyrGln: 1.176 ± 0.316
2.572TyrArg: 2.572 ± 0.355
2.058TyrSer: 2.058 ± 0.388
2.719TyrThr: 2.719 ± 0.385
2.646TyrVal: 2.646 ± 0.42
0.147TyrTrp: 0.147 ± 0.093
1.102TyrTyr: 1.102 ± 0.32
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 71 proteins (13609 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski