Salmonella phage FSLSP004

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Stockinghallvirus; Salmonella virus FSLSP004

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 40 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S4TNZ0|S4TNZ0_9CAUD Major tail tube protein OS=Salmonella phage FSLSP004 OX=1173769 GN=SP004_00105 PE=4 SV=1
MM1 pKa = 7.23ATVDD5 pKa = 4.28LSQLPVPDD13 pKa = 3.55VVEE16 pKa = 4.06EE17 pKa = 4.11LDD19 pKa = 3.72YY20 pKa = 10.57EE21 pKa = 4.61TILAEE26 pKa = 5.12RR27 pKa = 11.84IATLISLYY35 pKa = 10.45PEE37 pKa = 4.5DD38 pKa = 3.46QQEE41 pKa = 4.12AVARR45 pKa = 11.84TLALEE50 pKa = 4.08SEE52 pKa = 5.07PIVKK56 pKa = 10.22LLQEE60 pKa = 3.72NAYY63 pKa = 10.54RR64 pKa = 11.84EE65 pKa = 4.54VIWRR69 pKa = 11.84QRR71 pKa = 11.84VNEE74 pKa = 3.96AARR77 pKa = 11.84AVMLAYY83 pKa = 10.24AIDD86 pKa = 4.06SDD88 pKa = 4.56LDD90 pKa = 4.07NIGGNFSVEE99 pKa = 3.97RR100 pKa = 11.84LVVTPADD107 pKa = 3.87DD108 pKa = 3.72TTIPPTPAEE117 pKa = 4.27MEE119 pKa = 3.93LDD121 pKa = 2.96ADD123 pKa = 3.62YY124 pKa = 11.22RR125 pKa = 11.84LRR127 pKa = 11.84IQQAFEE133 pKa = 4.12GLSVAGPVGAYY144 pKa = 9.25QYY146 pKa = 10.44HH147 pKa = 6.16GRR149 pKa = 11.84SADD152 pKa = 3.24GRR154 pKa = 11.84VGDD157 pKa = 4.33ISVISPSPACVTISVLSRR175 pKa = 11.84EE176 pKa = 4.35NNGVASEE183 pKa = 4.1EE184 pKa = 3.82LLAIVRR190 pKa = 11.84NALNAEE196 pKa = 4.28DD197 pKa = 4.08VRR199 pKa = 11.84PVADD203 pKa = 3.2RR204 pKa = 11.84VTVQSAEE211 pKa = 3.77IVNYY215 pKa = 9.8QINATLYY222 pKa = 9.71LYY224 pKa = 8.88PGPEE228 pKa = 4.09SEE230 pKa = 5.15PIRR233 pKa = 11.84AAAEE237 pKa = 4.21AKK239 pKa = 10.05LKK241 pKa = 10.73AYY243 pKa = 9.88ISAQHH248 pKa = 6.43RR249 pKa = 11.84LGRR252 pKa = 11.84DD253 pKa = 2.91IRR255 pKa = 11.84KK256 pKa = 8.91SAIYY260 pKa = 9.91AALHH264 pKa = 5.3VEE266 pKa = 4.81GVQRR270 pKa = 11.84VEE272 pKa = 3.97LAAPVADD279 pKa = 2.95IVLDD283 pKa = 3.5NTQASFCTDD292 pKa = 3.12YY293 pKa = 11.51SLVIGGSDD301 pKa = 3.28EE302 pKa = 3.99

Molecular weight: 32.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S4TP00|S4TP00_9CAUD DUF5405 domain-containing protein OS=Salmonella phage FSLSP004 OX=1173769 GN=SP004_00180 PE=4 SV=1
MM1 pKa = 7.53AFVDD5 pKa = 4.75GVVIMNEE12 pKa = 3.97PRR14 pKa = 11.84CIAQLLRR21 pKa = 11.84NEE23 pKa = 4.34SPRR26 pKa = 11.84AIDD29 pKa = 3.27FTITHH34 pKa = 6.18GKK36 pKa = 7.96GRR38 pKa = 11.84KK39 pKa = 9.25GIIIRR44 pKa = 11.84TKK46 pKa = 10.04KK47 pKa = 10.34QSPLKK52 pKa = 10.41KK53 pKa = 10.19ALTFLKK59 pKa = 10.34SRR61 pKa = 11.84RR62 pKa = 11.84VWKK65 pKa = 10.53

Molecular weight: 7.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

40

0

40

9148

63

813

228.7

25.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.319 ± 0.519

0.918 ± 0.151

5.805 ± 0.305

6.067 ± 0.29

3.268 ± 0.297

6.909 ± 0.458

1.957 ± 0.214

5.367 ± 0.274

5.619 ± 0.398

9.062 ± 0.279

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.613 ± 0.14

4.077 ± 0.259

4.154 ± 0.276

3.99 ± 0.254

6.439 ± 0.509

6.176 ± 0.335

6.362 ± 0.407

6.679 ± 0.388

1.443 ± 0.14

2.777 ± 0.172
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski