Coprobacillus sp. CAG:826
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1394 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R7DLX8|R7DLX8_9FIRM Conserved domain protein OS=Coprobacillus sp. CAG:826 OX=1262857 GN=BN794_00034 PE=4 SV=1
MM1 pKa = 7.7 EE2 pKa = 5.71 PLDD5 pKa = 3.92 NEE7 pKa = 4.17 IVIVDD12 pKa = 3.63 EE13 pKa = 5.52 DD14 pKa = 3.97 GVEE17 pKa = 4.23 TKK19 pKa = 10.76 FEE21 pKa = 4.04 ILFTYY26 pKa = 10.51 EE27 pKa = 3.37 NDD29 pKa = 3.81 DD30 pKa = 3.89 RR31 pKa = 11.84 GTQYY35 pKa = 11.5 VLYY38 pKa = 9.44 YY39 pKa = 10.43 DD40 pKa = 3.95 PKK42 pKa = 10.02 NPEE45 pKa = 3.68 EE46 pKa = 4.19 VFAARR51 pKa = 11.84 YY52 pKa = 9.0 NDD54 pKa = 3.1 NHH56 pKa = 6.34 EE57 pKa = 4.19 LMEE60 pKa = 6.1 IEE62 pKa = 6.19 DD63 pKa = 3.79 EE64 pKa = 4.53 EE65 pKa = 4.29 EE66 pKa = 3.85 WQEE69 pKa = 4.11 VEE71 pKa = 4.28 EE72 pKa = 4.09 VLNTFEE78 pKa = 4.89 QDD80 pKa = 3.31 PAIQGALDD88 pKa = 3.53 EE89 pKa = 4.53 EE90 pKa = 4.81
Molecular weight: 10.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.877
IPC2_protein 3.567
IPC_protein 3.503
Toseland 3.325
ProMoST 3.63
Dawson 3.478
Bjellqvist 3.681
Wikipedia 3.376
Rodwell 3.338
Grimsley 3.236
Solomon 3.452
Lehninger 3.401
Nozaki 3.605
DTASelect 3.719
Thurlkill 3.376
EMBOSS 3.389
Sillero 3.617
Patrickios 1.736
IPC_peptide 3.452
IPC2_peptide 3.592
IPC2.peptide.svr19 3.658
Protein with the highest isoelectric point:
>tr|R7DQV9|R7DQV9_9FIRM Membrane protein insertase YidC/Oxa1 family OS=Coprobacillus sp. CAG:826 OX=1262857 GN=BN794_00897 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.14 QPSKK9 pKa = 9.79 RR10 pKa = 11.84 KK11 pKa = 8.83 HH12 pKa = 4.74 QATHH16 pKa = 5.86 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATPNGRR28 pKa = 11.84 KK29 pKa = 9.01 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.35 GRR39 pKa = 11.84 AVLSAA44 pKa = 3.96
Molecular weight: 5.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.387
IPC2_protein 11.038
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.281
Grimsley 12.603
Solomon 13.042
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.018
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 9.031
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1394
0
1394
475966
29
1868
341.4
38.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.963 ± 0.06
1.178 ± 0.025
5.517 ± 0.049
7.448 ± 0.061
4.928 ± 0.047
5.875 ± 0.053
2.184 ± 0.037
7.987 ± 0.061
7.978 ± 0.052
9.513 ± 0.069
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.712 ± 0.028
4.977 ± 0.054
3.057 ± 0.032
3.097 ± 0.032
3.568 ± 0.042
6.408 ± 0.064
5.595 ± 0.066
6.288 ± 0.048
0.825 ± 0.017
4.902 ± 0.046
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here