Soft-shelled turtle iridovirus

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Pimascovirales; Iridoviridae; Alphairidovirinae; Ranavirus; Frog virus 3

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 105 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C3RWT1|C3RWT1_FRG3V Putative membrane associated motif in LPS-induced tumor necrosis factor alpha OS=Soft-shelled turtle iridovirus OX=365144 GN=ORF082L PE=4 SV=1
MM1 pKa = 7.14VLLMSSVGLRR11 pKa = 11.84CLAALPYY18 pKa = 8.55TWRR21 pKa = 11.84LPGVICDD28 pKa = 3.63PWTGPTGTLDD38 pKa = 3.65SEE40 pKa = 4.99CPVATGSGALKK51 pKa = 10.64ASLCLEE57 pKa = 4.2YY58 pKa = 10.87AFSIPAGAGGGSLWAPDD75 pKa = 3.87PMPWTPLAVPPAMMLSAVVRR95 pKa = 11.84GARR98 pKa = 11.84LTALEE103 pKa = 4.12NWSLNPAWPGVWGLGVGSLRR123 pKa = 11.84TADD126 pKa = 4.71PPFPWEE132 pKa = 4.12PTSSMMGEE140 pKa = 4.11EE141 pKa = 4.5DD142 pKa = 4.43CLCGAA147 pKa = 5.24

Molecular weight: 15.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C3RWP9|C3RWP9_FRG3V Uncharacterized protein OS=Soft-shelled turtle iridovirus OX=365144 GN=ORF050L PE=4 SV=1
MM1 pKa = 7.11YY2 pKa = 10.24SVRR5 pKa = 11.84NSGCSAGCSPRR16 pKa = 11.84QGASPIMFGPSLGAMLSAPVVRR38 pKa = 11.84ASSPVVKK45 pKa = 10.32RR46 pKa = 11.84KK47 pKa = 10.35SLVKK51 pKa = 10.26RR52 pKa = 11.84KK53 pKa = 10.2SPVKK57 pKa = 10.34RR58 pKa = 11.84SPLKK62 pKa = 10.44KK63 pKa = 9.82RR64 pKa = 11.84SQTRR68 pKa = 11.84TSPVKK73 pKa = 10.24RR74 pKa = 11.84KK75 pKa = 10.42SPVKK79 pKa = 10.07RR80 pKa = 11.84KK81 pKa = 10.4SPVKK85 pKa = 10.07RR86 pKa = 11.84KK87 pKa = 10.4SPVKK91 pKa = 10.07RR92 pKa = 11.84KK93 pKa = 10.4SPVKK97 pKa = 10.07RR98 pKa = 11.84KK99 pKa = 10.4SPVKK103 pKa = 10.07RR104 pKa = 11.84KK105 pKa = 10.4SPVKK109 pKa = 10.07RR110 pKa = 11.84KK111 pKa = 10.4SPVKK115 pKa = 10.07RR116 pKa = 11.84KK117 pKa = 10.4SPVKK121 pKa = 10.07RR122 pKa = 11.84KK123 pKa = 10.4SPVKK127 pKa = 10.07RR128 pKa = 11.84KK129 pKa = 10.4SPVKK133 pKa = 10.07RR134 pKa = 11.84KK135 pKa = 10.4SPVKK139 pKa = 10.07RR140 pKa = 11.84KK141 pKa = 10.4SPVKK145 pKa = 10.07RR146 pKa = 11.84KK147 pKa = 10.4SPVKK151 pKa = 10.07RR152 pKa = 11.84KK153 pKa = 10.4SPVKK157 pKa = 10.07RR158 pKa = 11.84KK159 pKa = 10.4SPVKK163 pKa = 10.07RR164 pKa = 11.84KK165 pKa = 10.4SPVKK169 pKa = 10.07RR170 pKa = 11.84KK171 pKa = 10.4SPVKK175 pKa = 10.05RR176 pKa = 11.84KK177 pKa = 10.07SPAKK181 pKa = 10.0RR182 pKa = 11.84RR183 pKa = 11.84QQVCFMATKK192 pKa = 10.43

Molecular weight: 21.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

105

0

105

29450

42

1294

280.5

30.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.015 ± 0.275

2.105 ± 0.164

6.068 ± 0.152

5.657 ± 0.205

3.345 ± 0.115

7.324 ± 0.266

1.922 ± 0.128

3.284 ± 0.133

5.701 ± 0.309

8.143 ± 0.326

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.175 ± 0.127

2.608 ± 0.125

6.278 ± 0.308

2.635 ± 0.149

6.788 ± 0.3

7.535 ± 0.246

5.671 ± 0.179

8.323 ± 0.222

1.501 ± 0.109

2.924 ± 0.138
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski