Ostreococcus mediterraneus virus 1
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 249 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0P0C0U6|A0A0P0C0U6_9PHYC Uncharacterized protein OS=Ostreococcus mediterraneus virus 1 OX=1663210 GN=OmV1_112 PE=4 SV=1
MM1 pKa = 7.28 MKK3 pKa = 9.75 TGFGEE8 pKa = 4.13 SSGDD12 pKa = 3.65 YY13 pKa = 9.7 EE14 pKa = 5.51 ASQQQALMGILLPVLEE30 pKa = 5.06 RR31 pKa = 11.84 SMVMAAEE38 pKa = 4.1 YY39 pKa = 10.97 SKK41 pKa = 11.45 ACGRR45 pKa = 11.84 NTVLPEE51 pKa = 4.14 DD52 pKa = 3.44 MEE54 pKa = 4.46 YY55 pKa = 10.7 AIKK58 pKa = 10.22 YY59 pKa = 9.78 CVMHH63 pKa = 6.14 TVGQNIGSLFPEE75 pKa = 4.91 IYY77 pKa = 10.35 DD78 pKa = 3.76 EE79 pKa = 4.39 EE80 pKa = 5.69 SSDD83 pKa = 3.81 EE84 pKa = 4.28 EE85 pKa = 4.21 EE86 pKa = 4.79 LEE88 pKa = 4.13 EE89 pKa = 5.47 VPPEE93 pKa = 4.16 DD94 pKa = 3.83 CPPFEE99 pKa = 5.8 RR100 pKa = 11.84 YY101 pKa = 8.11 TGDD104 pKa = 3.2 DD105 pKa = 3.23 TTFRR109 pKa = 11.84 QMNEE113 pKa = 3.65 AYY115 pKa = 10.1 DD116 pKa = 4.02 RR117 pKa = 11.84 WEE119 pKa = 3.58 QWVPQSPVEE128 pKa = 4.04 EE129 pKa = 4.05 MLKK132 pKa = 10.56 NAINSNEE139 pKa = 4.05 YY140 pKa = 9.9 IGSGGLDD147 pKa = 3.37 DD148 pKa = 5.04 FF149 pKa = 5.68
Molecular weight: 16.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.775
IPC2_protein 3.973
IPC_protein 3.897
Toseland 3.732
ProMoST 4.012
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.719
Rodwell 3.732
Grimsley 3.643
Solomon 3.834
Lehninger 3.795
Nozaki 3.961
DTASelect 4.088
Thurlkill 3.745
EMBOSS 3.732
Sillero 4.012
Patrickios 1.036
IPC_peptide 3.846
IPC2_peptide 3.999
IPC2.peptide.svr19 3.897
Protein with the highest isoelectric point:
>tr|A0A0N7HVI7|A0A0N7HVI7_9PHYC Uncharacterized protein OS=Ostreococcus mediterraneus virus 1 OX=1663210 GN=OmV1_198c PE=3 SV=1
MM1 pKa = 7.41 IPVVNYY7 pKa = 10.35 GRR9 pKa = 11.84 MEE11 pKa = 4.21 RR12 pKa = 11.84 LRR14 pKa = 11.84 PPEE17 pKa = 3.89 RR18 pKa = 11.84 TSIPMNANTFAIAFIILCCLGLYY41 pKa = 9.66 KK42 pKa = 10.36 RR43 pKa = 11.84 AVTISQSRR51 pKa = 11.84 AQSYY55 pKa = 8.2 TLDD58 pKa = 3.06 TLMPTKK64 pKa = 10.35 RR65 pKa = 11.84 GLSSSISS72 pKa = 3.37
Molecular weight: 8.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.31
IPC2_protein 9.677
IPC_protein 10.452
Toseland 10.131
ProMoST 9.999
Dawson 10.394
Bjellqvist 10.189
Wikipedia 10.643
Rodwell 10.496
Grimsley 10.496
Solomon 10.467
Lehninger 10.423
Nozaki 10.218
DTASelect 10.16
Thurlkill 10.233
EMBOSS 10.555
Sillero 10.321
Patrickios 10.248
IPC_peptide 10.452
IPC2_peptide 9.472
IPC2.peptide.svr19 8.314
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
249
0
249
61481
64
3442
246.9
27.99
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.047 ± 0.28
1.737 ± 0.139
5.807 ± 0.148
6.594 ± 0.182
4.162 ± 0.152
6.382 ± 0.285
2.131 ± 0.123
6.095 ± 0.169
7.516 ± 0.386
7.973 ± 0.191
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.874 ± 0.111
5.802 ± 0.476
4.122 ± 0.179
3.453 ± 0.137
5.193 ± 0.184
5.908 ± 0.18
6.337 ± 0.222
6.774 ± 0.209
1.074 ± 0.077
4.019 ± 0.151
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here