Amino acid dipepetide frequency for Methylobacterium sp. TER-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
24.63AlaAla: 24.63 ± 0.18
1.281AlaCys: 1.281 ± 0.026
7.407AlaAsp: 7.407 ± 0.083
8.571AlaGlu: 8.571 ± 0.091
4.642AlaPhe: 4.642 ± 0.056
14.819AlaGly: 14.819 ± 0.133
2.347AlaHis: 2.347 ± 0.046
5.506AlaIle: 5.506 ± 0.062
2.904AlaLys: 2.904 ± 0.054
16.505AlaLeu: 16.505 ± 0.124
3.406AlaMet: 3.406 ± 0.049
2.33AlaAsn: 2.33 ± 0.049
7.862AlaPro: 7.862 ± 0.083
3.851AlaGln: 3.851 ± 0.052
12.184AlaArg: 12.184 ± 0.118
6.233AlaSer: 6.233 ± 0.072
6.938AlaThr: 6.938 ± 0.087
11.377AlaVal: 11.377 ± 0.094
1.7AlaTrp: 1.7 ± 0.036
2.676AlaTyr: 2.676 ± 0.047
0.0AlaXaa: 0.0 ± 0.0
Cys
1.002CysAla: 1.002 ± 0.027
0.08CysCys: 0.08 ± 0.007
0.478CysAsp: 0.478 ± 0.017
0.387CysGlu: 0.387 ± 0.015
0.251CysPhe: 0.251 ± 0.012
0.893CysGly: 0.893 ± 0.024
0.197CysHis: 0.197 ± 0.011
0.273CysIle: 0.273 ± 0.012
0.107CysLys: 0.107 ± 0.008
0.897CysLeu: 0.897 ± 0.024
0.11CysMet: 0.11 ± 0.008
0.167CysAsn: 0.167 ± 0.01
0.45CysPro: 0.45 ± 0.016
0.157CysGln: 0.157 ± 0.009
0.777CysArg: 0.777 ± 0.024
0.329CysSer: 0.329 ± 0.016
0.404CysThr: 0.404 ± 0.016
0.557CysVal: 0.557 ± 0.017
0.098CysTrp: 0.098 ± 0.008
0.142CysTyr: 0.142 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
7.787AspAla: 7.787 ± 0.078
0.388AspCys: 0.388 ± 0.015
3.126AspAsp: 3.126 ± 0.053
3.253AspGlu: 3.253 ± 0.053
1.879AspPhe: 1.879 ± 0.035
6.191AspGly: 6.191 ± 0.091
1.153AspHis: 1.153 ± 0.025
1.981AspIle: 1.981 ± 0.032
1.067AspLys: 1.067 ± 0.028
7.041AspLeu: 7.041 ± 0.074
0.824AspMet: 0.824 ± 0.02
0.906AspAsn: 0.906 ± 0.027
4.432AspPro: 4.432 ± 0.057
1.24AspGln: 1.24 ± 0.028
5.206AspArg: 5.206 ± 0.066
1.695AspSer: 1.695 ± 0.031
2.515AspThr: 2.515 ± 0.061
3.997AspVal: 3.997 ± 0.053
0.875AspTrp: 0.875 ± 0.024
1.231AspTyr: 1.231 ± 0.028
0.0AspXaa: 0.0 ± 0.0
Glu
9.434GluAla: 9.434 ± 0.095
0.286GluCys: 0.286 ± 0.013
2.421GluAsp: 2.421 ± 0.041
2.74GluGlu: 2.74 ± 0.052
1.349GluPhe: 1.349 ± 0.033
4.471GluGly: 4.471 ± 0.051
1.0GluHis: 1.0 ± 0.023
2.918GluIle: 2.918 ± 0.048
1.193GluLys: 1.193 ± 0.03
3.903GluLeu: 3.903 ± 0.053
1.175GluMet: 1.175 ± 0.026
0.999GluAsn: 0.999 ± 0.021
3.135GluPro: 3.135 ± 0.047
1.514GluGln: 1.514 ± 0.031
5.576GluArg: 5.576 ± 0.069
2.037GluSer: 2.037 ± 0.035
3.452GluThr: 3.452 ± 0.049
3.905GluVal: 3.905 ± 0.057
0.531GluTrp: 0.531 ± 0.016
0.759GluTyr: 0.759 ± 0.022
0.0GluXaa: 0.0 ± 0.0
Phe
4.643PheAla: 4.643 ± 0.052
0.323PheCys: 0.323 ± 0.012
2.216PheAsp: 2.216 ± 0.035
1.793PheGlu: 1.793 ± 0.033
1.098PhePhe: 1.098 ± 0.029
3.633PheGly: 3.633 ± 0.054
0.628PheHis: 0.628 ± 0.021
0.993PheIle: 0.993 ± 0.025
0.675PheLys: 0.675 ± 0.022
3.236PheLeu: 3.236 ± 0.047
0.558PheMet: 0.558 ± 0.02
0.783PheAsn: 0.783 ± 0.021
1.52PhePro: 1.52 ± 0.029
0.841PheGln: 0.841 ± 0.02
2.496PheArg: 2.496 ± 0.041
1.828PheSer: 1.828 ± 0.034
1.765PheThr: 1.765 ± 0.037
2.768PheVal: 2.768 ± 0.042
0.447PheTrp: 0.447 ± 0.015
0.777PheTyr: 0.777 ± 0.019
0.0PheXaa: 0.0 ± 0.0
Gly
11.834GlyAla: 11.834 ± 0.117
0.828GlyCys: 0.828 ± 0.022
4.874GlyAsp: 4.874 ± 0.076
5.07GlyGlu: 5.07 ± 0.055
3.7GlyPhe: 3.7 ± 0.048
9.181GlyGly: 9.181 ± 0.178
1.992GlyHis: 1.992 ± 0.032
4.194GlyIle: 4.194 ± 0.053
2.064GlyLys: 2.064 ± 0.042
11.437GlyLeu: 11.437 ± 0.11
1.889GlyMet: 1.889 ± 0.032
1.868GlyAsn: 1.868 ± 0.051
5.047GlyPro: 5.047 ± 0.058
2.798GlyGln: 2.798 ± 0.041
8.902GlyArg: 8.902 ± 0.074
4.758GlySer: 4.758 ± 0.082
5.542GlyThr: 5.542 ± 0.122
6.33GlyVal: 6.33 ± 0.057
1.384GlyTrp: 1.384 ± 0.032
2.218GlyTyr: 2.218 ± 0.039
0.0GlyXaa: 0.0 ± 0.0
His
2.534HisAla: 2.534 ± 0.039
0.2HisCys: 0.2 ± 0.012
1.241HisAsp: 1.241 ± 0.029
0.912HisGlu: 0.912 ± 0.022
0.656HisPhe: 0.656 ± 0.021
2.108HisGly: 2.108 ± 0.04
0.555HisHis: 0.555 ± 0.02
0.571HisIle: 0.571 ± 0.019
0.302HisLys: 0.302 ± 0.012
2.153HisLeu: 2.153 ± 0.041
0.333HisMet: 0.333 ± 0.013
0.343HisAsn: 0.343 ± 0.015
1.463HisPro: 1.463 ± 0.033
0.443HisGln: 0.443 ± 0.017
1.718HisArg: 1.718 ± 0.033
0.671HisSer: 0.671 ± 0.022
0.696HisThr: 0.696 ± 0.019
1.436HisVal: 1.436 ± 0.031
0.298HisTrp: 0.298 ± 0.012
0.493HisTyr: 0.493 ± 0.017
0.0HisXaa: 0.0 ± 0.0
Ile
6.117IleAla: 6.117 ± 0.068
0.328IleCys: 0.328 ± 0.014
2.871IleAsp: 2.871 ± 0.042
2.848IleGlu: 2.848 ± 0.043
1.061IlePhe: 1.061 ± 0.025
4.259IleGly: 4.259 ± 0.06
0.75IleHis: 0.75 ± 0.023
1.18IleIle: 1.18 ± 0.03
0.853IleLys: 0.853 ± 0.023
3.948IleLeu: 3.948 ± 0.051
0.555IleMet: 0.555 ± 0.018
0.923IleAsn: 0.923 ± 0.025
2.043IlePro: 2.043 ± 0.04
0.977IleGln: 0.977 ± 0.024
3.221IleArg: 3.221 ± 0.04
1.694IleSer: 1.694 ± 0.037
2.036IleThr: 2.036 ± 0.037
3.548IleVal: 3.548 ± 0.051
0.406IleTrp: 0.406 ± 0.015
0.801IleTyr: 0.801 ± 0.022
0.0IleXaa: 0.0 ± 0.0
Lys
3.171LysAla: 3.171 ± 0.056
0.093LysCys: 0.093 ± 0.007
1.139LysAsp: 1.139 ± 0.031
0.92LysGlu: 0.92 ± 0.027
0.542LysPhe: 0.542 ± 0.017
1.933LysGly: 1.933 ± 0.038
0.335LysHis: 0.335 ± 0.015
0.961LysIle: 0.961 ± 0.026
0.621LysLys: 0.621 ± 0.024
1.958LysLeu: 1.958 ± 0.035
0.349LysMet: 0.349 ± 0.013
0.496LysAsn: 0.496 ± 0.019
1.598LysPro: 1.598 ± 0.036
0.598LysGln: 0.598 ± 0.021
1.631LysArg: 1.631 ± 0.036
1.07LysSer: 1.07 ± 0.026
1.227LysThr: 1.227 ± 0.027
1.687LysVal: 1.687 ± 0.037
0.217LysTrp: 0.217 ± 0.011
0.387LysTyr: 0.387 ± 0.016
0.0LysXaa: 0.0 ± 0.0
Leu
17.89LeuAla: 17.89 ± 0.15
0.906LeuCys: 0.906 ± 0.026
7.246LeuAsp: 7.246 ± 0.065
4.195LeuGlu: 4.195 ± 0.057
3.39LeuPhe: 3.39 ± 0.049
9.579LeuGly: 9.579 ± 0.098
1.938LeuHis: 1.938 ± 0.043
4.065LeuIle: 4.065 ± 0.054
2.521LeuLys: 2.521 ± 0.047
9.734LeuLeu: 9.734 ± 0.102
1.948LeuMet: 1.948 ± 0.038
2.038LeuAsn: 2.038 ± 0.038
6.269LeuPro: 6.269 ± 0.067
2.321LeuGln: 2.321 ± 0.038
8.423LeuArg: 8.423 ± 0.08
5.887LeuSer: 5.887 ± 0.065
5.811LeuThr: 5.811 ± 0.08
8.892LeuVal: 8.892 ± 0.084
1.173LeuTrp: 1.173 ± 0.025
1.974LeuTyr: 1.974 ± 0.035
0.0LeuXaa: 0.0 ± 0.0
Met
2.627MetAla: 2.627 ± 0.046
0.102MetCys: 0.102 ± 0.007
0.796MetAsp: 0.796 ± 0.024
0.773MetGlu: 0.773 ± 0.024
0.44MetPhe: 0.44 ± 0.017
1.364MetGly: 1.364 ± 0.03
0.345MetHis: 0.345 ± 0.013
0.952MetIle: 0.952 ± 0.026
0.55MetLys: 0.55 ± 0.017
2.002MetLeu: 2.002 ± 0.035
0.471MetMet: 0.471 ± 0.017
0.504MetAsn: 0.504 ± 0.015
1.413MetPro: 1.413 ± 0.028
0.598MetGln: 0.598 ± 0.018
1.832MetArg: 1.832 ± 0.035
1.273MetSer: 1.273 ± 0.029
1.588MetThr: 1.588 ± 0.029
1.351MetVal: 1.351 ± 0.022
0.128MetTrp: 0.128 ± 0.009
0.198MetTyr: 0.198 ± 0.011
0.0MetXaa: 0.0 ± 0.0
Asn
2.616AsnAla: 2.616 ± 0.043
0.167AsnCys: 0.167 ± 0.009
1.136AsnAsp: 1.136 ± 0.036
0.819AsnGlu: 0.819 ± 0.025
0.625AsnPhe: 0.625 ± 0.021
1.927AsnGly: 1.927 ± 0.043
0.373AsnHis: 0.373 ± 0.017
0.742AsnIle: 0.742 ± 0.021
0.428AsnLys: 0.428 ± 0.018
2.231AsnLeu: 2.231 ± 0.04
0.344AsnMet: 0.344 ± 0.015
0.483AsnAsn: 0.483 ± 0.018
1.588AsnPro: 1.588 ± 0.035
0.568AsnGln: 0.568 ± 0.019
1.611AsnArg: 1.611 ± 0.029
0.789AsnSer: 0.789 ± 0.025
0.966AsnThr: 0.966 ± 0.024
1.492AsnVal: 1.492 ± 0.031
0.289AsnTrp: 0.289 ± 0.012
0.515AsnTyr: 0.515 ± 0.021
0.0AsnXaa: 0.0 ± 0.0
Pro
9.317ProAla: 9.317 ± 0.086
0.336ProCys: 0.336 ± 0.013
4.495ProAsp: 4.495 ± 0.059
4.073ProGlu: 4.073 ± 0.053
1.967ProPhe: 1.967 ± 0.036
6.35ProGly: 6.35 ± 0.062
1.146ProHis: 1.146 ± 0.027
1.892ProIle: 1.892 ± 0.035
1.239ProLys: 1.239 ± 0.031
5.265ProLeu: 5.265 ± 0.056
1.122ProMet: 1.122 ± 0.027
1.137ProAsn: 1.137 ± 0.026
3.724ProPro: 3.724 ± 0.061
1.443ProGln: 1.443 ± 0.027
4.113ProArg: 4.113 ± 0.054
2.756ProSer: 2.756 ± 0.041
2.543ProThr: 2.543 ± 0.035
5.23ProVal: 5.23 ± 0.063
0.765ProTrp: 0.765 ± 0.022
1.139ProTyr: 1.139 ± 0.026
0.0ProXaa: 0.0 ± 0.0
Gln
4.059GlnAla: 4.059 ± 0.052
0.148GlnCys: 0.148 ± 0.01
1.421GlnAsp: 1.421 ± 0.027
1.2GlnGlu: 1.2 ± 0.027
0.796GlnPhe: 0.796 ± 0.023
2.404GlnGly: 2.404 ± 0.039
0.48GlnHis: 0.48 ± 0.016
1.377GlnIle: 1.377 ± 0.031
0.649GlnLys: 0.649 ± 0.019
2.073GlnLeu: 2.073 ± 0.038
0.552GlnMet: 0.552 ± 0.017
0.652GlnAsn: 0.652 ± 0.02
1.56GlnPro: 1.56 ± 0.03
0.812GlnGln: 0.812 ± 0.027
2.131GlnArg: 2.131 ± 0.036
1.346GlnSer: 1.346 ± 0.032
1.479GlnThr: 1.479 ± 0.035
2.113GlnVal: 2.113 ± 0.038
0.302GlnTrp: 0.302 ± 0.014
0.469GlnTyr: 0.469 ± 0.014
0.0GlnXaa: 0.0 ± 0.0
Arg
10.898ArgAla: 10.898 ± 0.09
0.552ArgCys: 0.552 ± 0.019
5.081ArgAsp: 5.081 ± 0.063
4.641ArgGlu: 4.641 ± 0.066
3.203ArgPhe: 3.203 ± 0.044
6.28ArgGly: 6.28 ± 0.058
2.08ArgHis: 2.08 ± 0.04
4.172ArgIle: 4.172 ± 0.051
1.534ArgLys: 1.534 ± 0.032
10.233ArgLeu: 10.233 ± 0.097
1.773ArgMet: 1.773 ± 0.033
1.616ArgAsn: 1.616 ± 0.032
5.376ArgPro: 5.376 ± 0.065
2.43ArgGln: 2.43 ± 0.035
8.27ArgArg: 8.27 ± 0.096
3.897ArgSer: 3.897 ± 0.05
4.108ArgThr: 4.108 ± 0.053
5.98ArgVal: 5.98 ± 0.065
1.002ArgTrp: 1.002 ± 0.025
1.724ArgTyr: 1.724 ± 0.028
0.0ArgXaa: 0.0 ± 0.0
Ser
5.818SerAla: 5.818 ± 0.058
0.361SerCys: 0.361 ± 0.016
2.464SerAsp: 2.464 ± 0.037
2.219SerGlu: 2.219 ± 0.036
1.879SerPhe: 1.879 ± 0.035
5.397SerGly: 5.397 ± 0.088
0.863SerHis: 0.863 ± 0.022
1.818SerIle: 1.818 ± 0.038
0.918SerLys: 0.918 ± 0.022
5.316SerLeu: 5.316 ± 0.063
0.824SerMet: 0.824 ± 0.022
0.962SerAsn: 0.962 ± 0.029
2.867SerPro: 2.867 ± 0.04
1.265SerGln: 1.265 ± 0.029
3.668SerArg: 3.668 ± 0.046
2.149SerSer: 2.149 ± 0.044
2.283SerThr: 2.283 ± 0.042
3.599SerVal: 3.599 ± 0.056
0.605SerTrp: 0.605 ± 0.017
1.122SerTyr: 1.122 ± 0.034
0.0SerXaa: 0.0 ± 0.0
Thr
6.894ThrAla: 6.894 ± 0.089
0.398ThrCys: 0.398 ± 0.015
2.575ThrAsp: 2.575 ± 0.045
2.301ThrGlu: 2.301 ± 0.04
1.75ThrPhe: 1.75 ± 0.033
5.807ThrGly: 5.807 ± 0.116
0.931ThrHis: 0.931 ± 0.02
2.491ThrIle: 2.491 ± 0.042
0.983ThrLys: 0.983 ± 0.028
6.296ThrLeu: 6.296 ± 0.081
0.937ThrMet: 0.937 ± 0.026
1.047ThrAsn: 1.047 ± 0.033
3.351ThrPro: 3.351 ± 0.043
1.218ThrGln: 1.218 ± 0.029
3.862ThrArg: 3.862 ± 0.051
2.343ThrSer: 2.343 ± 0.049
2.874ThrThr: 2.874 ± 0.059
4.901ThrVal: 4.901 ± 0.08
0.66ThrTrp: 0.66 ± 0.019
1.197ThrTyr: 1.197 ± 0.032
0.0ThrXaa: 0.0 ± 0.0
Val
11.951ValAla: 11.951 ± 0.09
0.639ValCys: 0.639 ± 0.018
3.828ValAsp: 3.828 ± 0.047
4.599ValGlu: 4.599 ± 0.055
2.616ValPhe: 2.616 ± 0.04
6.193ValGly: 6.193 ± 0.064
1.313ValHis: 1.313 ± 0.026
3.183ValIle: 3.183 ± 0.046
1.629ValLys: 1.629 ± 0.037
8.351ValLeu: 8.351 ± 0.073
1.571ValMet: 1.571 ± 0.028
1.669ValAsn: 1.669 ± 0.037
4.649ValPro: 4.649 ± 0.059
2.003ValGln: 2.003 ± 0.035
6.009ValArg: 6.009 ± 0.064
4.03ValSer: 4.03 ± 0.061
4.813ValThr: 4.813 ± 0.092
7.185ValVal: 7.185 ± 0.083
0.867ValTrp: 0.867 ± 0.024
1.414ValTyr: 1.414 ± 0.029
0.0ValXaa: 0.0 ± 0.0
Trp
1.211TrpAla: 1.211 ± 0.027
0.144TrpCys: 0.144 ± 0.009
0.604TrpAsp: 0.604 ± 0.019
0.465TrpGlu: 0.465 ± 0.016
0.457TrpPhe: 0.457 ± 0.015
0.892TrpGly: 0.892 ± 0.023
0.318TrpHis: 0.318 ± 0.013
0.589TrpIle: 0.589 ± 0.017
0.295TrpLys: 0.295 ± 0.013
1.486TrpLeu: 1.486 ± 0.034
0.273TrpMet: 0.273 ± 0.012
0.341TrpAsn: 0.341 ± 0.014
0.702TrpPro: 0.702 ± 0.025
0.444TrpGln: 0.444 ± 0.016
1.334TrpArg: 1.334 ± 0.03
0.767TrpSer: 0.767 ± 0.023
0.786TrpThr: 0.786 ± 0.025
0.678TrpVal: 0.678 ± 0.02
0.209TrpTrp: 0.209 ± 0.011
0.251TrpTyr: 0.251 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.735TyrAla: 2.735 ± 0.045
0.197TyrCys: 0.197 ± 0.01
1.368TyrAsp: 1.368 ± 0.029
1.021TyrGlu: 1.021 ± 0.024
0.721TyrPhe: 0.721 ± 0.021
2.226TyrGly: 2.226 ± 0.039
0.38TyrHis: 0.38 ± 0.016
0.56TyrIle: 0.56 ± 0.021
0.403TyrLys: 0.403 ± 0.016
2.134TyrLeu: 2.134 ± 0.035
0.292TyrMet: 0.292 ± 0.014
0.448TyrAsn: 0.448 ± 0.018
1.004TyrPro: 1.004 ± 0.023
0.502TyrGln: 0.502 ± 0.016
1.885TyrArg: 1.885 ± 0.036
0.845TyrSer: 0.845 ± 0.021
0.99TyrThr: 0.99 ± 0.032
1.484TyrVal: 1.484 ± 0.03
0.291TyrTrp: 0.291 ± 0.014
0.487TyrTyr: 0.487 ± 0.016
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5625 proteins (1810136 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski