Escherichia phage JES2013

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Vequintavirinae; Vequintavirus; Escherichia virus JES2013

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 220 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S4USI9|S4USI9_9CAUD Uncharacterized protein OS=Escherichia phage JES2013 OX=1327956 GN=Ec2_00113 PE=4 SV=1
MM1 pKa = 7.21SHH3 pKa = 5.63NSKK6 pKa = 10.11WKK8 pKa = 10.08DD9 pKa = 3.03LGFASRR15 pKa = 11.84AEE17 pKa = 4.05YY18 pKa = 10.81VGWLWFNGLTNDD30 pKa = 2.83IKK32 pKa = 10.85MYY34 pKa = 10.94DD35 pKa = 3.37DD36 pKa = 3.82VYY38 pKa = 10.64YY39 pKa = 10.79DD40 pKa = 3.95EE41 pKa = 4.54YY42 pKa = 9.79TGKK45 pKa = 10.35IINISVEE52 pKa = 3.99EE53 pKa = 4.12DD54 pKa = 3.28TFIQQDD60 pKa = 3.24TDD62 pKa = 3.45TCLDD66 pKa = 3.57KK67 pKa = 11.34YY68 pKa = 10.71VSEE71 pKa = 4.33VEE73 pKa = 4.15EE74 pKa = 4.43ANN76 pKa = 3.5

Molecular weight: 8.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S4USL6|S4USL6_9CAUD Uncharacterized protein OS=Escherichia phage JES2013 OX=1327956 GN=Ec2_00203 PE=4 SV=1
MM1 pKa = 7.16NAYY4 pKa = 9.55GIKK7 pKa = 9.91VRR9 pKa = 11.84RR10 pKa = 11.84NTRR13 pKa = 11.84QVVTDD18 pKa = 3.45QVVCLVDD25 pKa = 3.2VTINNGQGLYY35 pKa = 10.34KK36 pKa = 10.45FRR38 pKa = 11.84RR39 pKa = 11.84GHH41 pKa = 6.66SYY43 pKa = 9.31TLQQLNRR50 pKa = 11.84CALTKK55 pKa = 10.63GQINHH60 pKa = 6.33WFNKK64 pKa = 9.06KK65 pKa = 9.61

Molecular weight: 7.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

220

0

220

41316

29

1841

187.8

21.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.951 ± 0.192

1.634 ± 0.092

6.852 ± 0.134

7.07 ± 0.24

4.248 ± 0.1

7.029 ± 0.201

1.973 ± 0.082

5.947 ± 0.141

6.934 ± 0.225

7.728 ± 0.192

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.868 ± 0.102

4.94 ± 0.135

3.788 ± 0.111

3.214 ± 0.121

4.507 ± 0.121

5.531 ± 0.156

5.707 ± 0.245

7.089 ± 0.138

1.793 ± 0.098

4.197 ± 0.129
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski