[Ruminococcus] torques CAG:61

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Ruminococcus; environmental samples

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2019 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5QXW9|R5QXW9_9FIRM Fumerase domain-containing protein OS=[Ruminococcus] torques CAG:61 OX=1263108 GN=BN734_01421 PE=3 SV=1
MM1 pKa = 7.34KK2 pKa = 10.24FIYY5 pKa = 9.73PAVFRR10 pKa = 11.84KK11 pKa = 10.13NEE13 pKa = 4.01SGGYY17 pKa = 9.21DD18 pKa = 3.13AYY20 pKa = 11.04FPDD23 pKa = 5.22LEE25 pKa = 4.41CCEE28 pKa = 4.47ASGDD32 pKa = 4.03TLDD35 pKa = 5.67DD36 pKa = 5.4AIDD39 pKa = 3.95NANEE43 pKa = 3.86AARR46 pKa = 11.84NWIMVEE52 pKa = 4.02FEE54 pKa = 4.17EE55 pKa = 4.52EE56 pKa = 4.3NPVFPYY62 pKa = 10.41ISDD65 pKa = 3.42INDD68 pKa = 3.14IEE70 pKa = 4.42TEE72 pKa = 3.81AGDD75 pKa = 3.41IVRR78 pKa = 11.84NISVNIRR85 pKa = 11.84FYY87 pKa = 11.03EE88 pKa = 4.51GWDD91 pKa = 3.35EE92 pKa = 4.06

Molecular weight: 10.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5QAA8|R5QAA8_9FIRM HTH marR-type domain-containing protein OS=[Ruminococcus] torques CAG:61 OX=1263108 GN=BN734_01352 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 9.06KK9 pKa = 8.03RR10 pKa = 11.84SRR12 pKa = 11.84SKK14 pKa = 9.44VHH16 pKa = 6.17GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTPGGRR28 pKa = 11.84KK29 pKa = 8.8VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.18GRR39 pKa = 11.84KK40 pKa = 8.18QLSAA44 pKa = 3.9

Molecular weight: 5.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2019

0

2019

674015

29

4107

333.8

37.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.556 ± 0.056

1.375 ± 0.024

5.648 ± 0.05

8.277 ± 0.073

4.025 ± 0.039

7.286 ± 0.058

1.64 ± 0.023

7.333 ± 0.061

7.485 ± 0.047

8.512 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.046 ± 0.034

4.245 ± 0.046

3.131 ± 0.028

3.051 ± 0.032

4.325 ± 0.057

5.67 ± 0.044

5.494 ± 0.067

7.09 ± 0.046

0.811 ± 0.017

4.001 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski