Lysobacter daejeonensis GH1-9
Average proteome isoelectric point is 6.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2570 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A0ETD8|A0A0A0ETD8_9GAMM LacI family transcriptional regulator OS=Lysobacter daejeonensis GH1-9 OX=1385517 GN=N800_07390 PE=4 SV=1
MM1 pKa = 7.72 ALACLLLMVSATPAAQAQTVTATNSLSCLANRR33 pKa = 11.84 MDD35 pKa = 3.67 NPTNLGCTANDD46 pKa = 3.68 FSHH49 pKa = 7.05 IVTAQALSAPTTCTAGSEE67 pKa = 4.22 ITVSVQLTLQSNSTDD82 pKa = 3.34 RR83 pKa = 11.84 YY84 pKa = 10.89 DD85 pKa = 3.18 IGLFMGEE92 pKa = 4.89 DD93 pKa = 3.95 GNNPAQLGGTCSVATFPEE111 pKa = 4.81 SPAPFADD118 pKa = 4.33 LDD120 pKa = 4.25 AGTQPTDD127 pKa = 2.79 TCGDD131 pKa = 3.83 FNANGSATVTVQNVKK146 pKa = 8.69 LTCTAGADD154 pKa = 3.89 GKK156 pKa = 11.16 LVMPYY161 pKa = 9.69 TLAYY165 pKa = 9.03 SQNTGGVCTGPGVVRR180 pKa = 11.84 AGTTSKK186 pKa = 9.87 CTGGTASVSNIVVAGHH202 pKa = 6.26 VDD204 pKa = 3.45 VTKK207 pKa = 9.62 QTLPDD212 pKa = 3.85 AAPDD216 pKa = 3.7 SFAFTAVDD224 pKa = 4.66 GPDD227 pKa = 3.34 TSPTSFNLVDD237 pKa = 3.94 GGTQRR242 pKa = 11.84 VTTTFGATGSSITIDD257 pKa = 3.64 EE258 pKa = 4.43 NVPPHH263 pKa = 6.12 WRR265 pKa = 11.84 SSVACASSNVDD276 pKa = 2.98 TSGQANGTFIANLTSAEE293 pKa = 4.02 PTANCTVTNTKK304 pKa = 10.09 RR305 pKa = 11.84 SRR307 pKa = 11.84 ITLVKK312 pKa = 10.57 NIVGGRR318 pKa = 11.84 NNAADD323 pKa = 3.63 QFTVSGTGGGTLTNTSDD340 pKa = 3.58 VTMSAPVSATTSGTSTTASVTFYY363 pKa = 10.82 SSPGSGLTLTDD374 pKa = 3.04 AMAAGSVSTLADD386 pKa = 3.34 YY387 pKa = 9.7 TPTYY391 pKa = 10.68 SCTNATAGSPTSMPSGSGATFGLTPAPGDD420 pKa = 5.15 DD421 pKa = 3.52 ITCTYY426 pKa = 10.3 TNTLIARR433 pKa = 11.84 PSLLLDD439 pKa = 3.32 KK440 pKa = 10.66 RR441 pKa = 11.84 LVSGAGFDD449 pKa = 3.74 SVGDD453 pKa = 4.05 VIQYY457 pKa = 10.88 AYY459 pKa = 10.84 DD460 pKa = 3.47 VTNSGNVTITGPITVSDD477 pKa = 4.13 DD478 pKa = 3.62 KK479 pKa = 10.9 IASVTCPAGDD489 pKa = 4.08 LAPGAMITCTASYY502 pKa = 9.49 TVAQADD508 pKa = 3.81 INNGSVTNVATATGSFNNQPITSNADD534 pKa = 3.36 TVTVEE539 pKa = 4.15 ALQEE543 pKa = 3.98 PALTLAKK550 pKa = 9.32 TAVSGDD556 pKa = 3.88 PYY558 pKa = 11.41 DD559 pKa = 4.46 SIGDD563 pKa = 3.81 VVQYY567 pKa = 10.51 EE568 pKa = 4.29 YY569 pKa = 11.44 LVTNSGNVVITDD581 pKa = 5.42 PITVADD587 pKa = 4.67 DD588 pKa = 3.66 KK589 pKa = 10.61 TTVTCPALPGGGLAPGGSITCTASYY614 pKa = 9.82 TITQADD620 pKa = 3.37 VDD622 pKa = 4.25 AGSVTNLATATSGTVNSNQATEE644 pKa = 4.4 TVNATRR650 pKa = 11.84 SPSLGLEE657 pKa = 4.18 KK658 pKa = 9.88 TASPLVYY665 pKa = 10.05 AAAGDD670 pKa = 4.15 VISYY674 pKa = 10.61 SYY676 pKa = 11.63 LLTNTGNVTLSAPYY690 pKa = 9.2 TVADD694 pKa = 4.41 DD695 pKa = 4.2 KK696 pKa = 10.68 TTVTCPATPGTLAPGGTVTCTATYY720 pKa = 9.36 TITQTDD726 pKa = 3.69 VNAGSVTNVATASAIFNNAAVNSAQDD752 pKa = 3.58 SATVTATQTPTLSLDD767 pKa = 3.06 KK768 pKa = 10.63 RR769 pKa = 11.84 LVSGAGFDD777 pKa = 3.74 SVGDD781 pKa = 4.05 VIQYY785 pKa = 10.88 AYY787 pKa = 10.84 DD788 pKa = 3.47 VTNSGNVTITGPITVSDD805 pKa = 4.13 DD806 pKa = 3.62 KK807 pKa = 10.9 IASVTCPAGDD817 pKa = 4.08 LAPGAMITCTASYY830 pKa = 9.49 TVAQADD836 pKa = 3.81 INNGSVTNVATATGSFNNQPITSNADD862 pKa = 3.36 TVTVEE867 pKa = 4.15 ALQEE871 pKa = 3.98 PALTLAKK878 pKa = 9.32 TAVSGDD884 pKa = 3.88 PYY886 pKa = 11.41 DD887 pKa = 4.46 SIGDD891 pKa = 3.81 VVQYY895 pKa = 10.51 EE896 pKa = 4.29 YY897 pKa = 11.44 LVTNSGNVVITDD909 pKa = 5.42 PITVADD915 pKa = 4.67 DD916 pKa = 3.66 KK917 pKa = 10.61 TTVTCPALPGGGLAPGGSITCTASYY942 pKa = 9.82 TITQADD948 pKa = 3.37 VDD950 pKa = 4.25 AGSVTNLATATSGTVNSNQATEE972 pKa = 4.4 TVNATRR978 pKa = 11.84 SPSLGLEE985 pKa = 4.18 KK986 pKa = 9.88 TASPLVYY993 pKa = 10.05 AAAGDD998 pKa = 4.15 VISYY1002 pKa = 10.61 SYY1004 pKa = 11.63 LLTNTGNVTLSAPYY1018 pKa = 9.2 TVADD1022 pKa = 4.41 DD1023 pKa = 4.2 KK1024 pKa = 10.68 TTVTCPATPGTLAPGGTVTCTATYY1048 pKa = 9.36 TITQTDD1054 pKa = 3.69 VNAGSVTNVATASAIFNNAAVNSAQDD1080 pKa = 3.58 SATVTATQTPTLSLDD1095 pKa = 3.06 KK1096 pKa = 10.63 RR1097 pKa = 11.84 LVSGAGFDD1105 pKa = 3.74 SVGDD1109 pKa = 4.05 VIQYY1113 pKa = 10.88 AYY1115 pKa = 10.84 DD1116 pKa = 3.47 VTNSGNVTITGPITISDD1133 pKa = 3.9 DD1134 pKa = 3.72 KK1135 pKa = 10.6 IASVTCPAGDD1145 pKa = 4.08 LAPGAMVTCTASYY1158 pKa = 9.77 TVTQADD1164 pKa = 3.93 INTGSVTNIATAAGSFNNQPVTSNPDD1190 pKa = 3.38 TVTVEE1195 pKa = 4.06 ALQEE1199 pKa = 3.98 PALTLAKK1206 pKa = 9.32 TAVSGDD1212 pKa = 3.95 PYY1214 pKa = 11.28 DD1215 pKa = 3.51 SVGDD1219 pKa = 3.88 VVQYY1223 pKa = 10.7 EE1224 pKa = 4.27 YY1225 pKa = 11.44 LVTNSGNVVITDD1237 pKa = 5.42 PITVADD1243 pKa = 4.67 DD1244 pKa = 3.66 KK1245 pKa = 10.61 TTVTCPALPGGGLAPGGSITCTASYY1270 pKa = 9.82 TITQADD1276 pKa = 3.37 VDD1278 pKa = 4.25 AGSVTNLATATSGTVNSNQATEE1300 pKa = 4.4 TVNATRR1306 pKa = 11.84 SPSLGLEE1313 pKa = 4.18 KK1314 pKa = 9.88 TASPLVYY1321 pKa = 10.05 AAAGDD1326 pKa = 4.15 VISYY1330 pKa = 10.61 SYY1332 pKa = 11.63 LLTNTGNVTLSAPYY1346 pKa = 9.2 TVADD1350 pKa = 4.41 DD1351 pKa = 4.2 KK1352 pKa = 10.68 TTVTCPATPGTLAPGGTVTCTATYY1376 pKa = 9.36 TITQTDD1382 pKa = 3.69 VNAGSVTNVATASAIFNNAAVNSAQDD1408 pKa = 3.58 SATVTATQTPTLSLDD1423 pKa = 3.06 KK1424 pKa = 10.63 RR1425 pKa = 11.84 LVSGAGFDD1433 pKa = 3.74 SVGDD1437 pKa = 4.05 VIQYY1441 pKa = 10.88 AYY1443 pKa = 10.84 DD1444 pKa = 3.47 VTNSGNVTITGPITISDD1461 pKa = 3.9 DD1462 pKa = 3.72 KK1463 pKa = 10.6 IASVTCPAGDD1473 pKa = 4.08 LAPGAMVTCTASYY1486 pKa = 9.77 TVTQADD1492 pKa = 3.93 INTGSVTNIATAAGSFNNQPVTSNPDD1518 pKa = 3.38 TVTVEE1523 pKa = 4.06 ALQEE1527 pKa = 3.87 PALTLDD1533 pKa = 3.41 KK1534 pKa = 10.69 RR1535 pKa = 11.84 VVSGNPYY1542 pKa = 10.64 DD1543 pKa = 3.65 SVGDD1547 pKa = 3.93 VVQYY1551 pKa = 10.6 TYY1553 pKa = 11.62 DD1554 pKa = 3.42 VTNTGNLTITDD1565 pKa = 5.9 PITVSDD1571 pKa = 4.4 DD1572 pKa = 3.58 KK1573 pKa = 11.08 IATVNCPALPSGGLAPGAMLTCTASYY1599 pKa = 9.8 TITQADD1605 pKa = 3.37 VDD1607 pKa = 4.25 AGSVTNLATATSGTVNSNQATEE1629 pKa = 4.4 TVNATRR1635 pKa = 11.84 SPSLGLEE1642 pKa = 4.18 KK1643 pKa = 9.88 TASPLVYY1650 pKa = 10.05 AAAGDD1655 pKa = 4.15 VISYY1659 pKa = 10.61 SYY1661 pKa = 11.63 LLTNTGNVTLSAPYY1675 pKa = 9.2 TVADD1679 pKa = 4.41 DD1680 pKa = 4.2 KK1681 pKa = 10.68 TTVTCPATPGTLAPGGTVTCTATYY1705 pKa = 9.36 TITQTDD1711 pKa = 3.69 VNAGSVTNVATASAIFNNAAVNSAQDD1737 pKa = 3.58 SATVTATQTPTLSLDD1752 pKa = 3.06 KK1753 pKa = 10.63 RR1754 pKa = 11.84 LVSGAGFDD1762 pKa = 3.74 SVGDD1766 pKa = 4.05 VIQYY1770 pKa = 10.88 AYY1772 pKa = 10.84 DD1773 pKa = 3.47 VTNSGNVTITGPITISDD1790 pKa = 3.9 DD1791 pKa = 3.72 KK1792 pKa = 10.6 IASVTCPAGDD1802 pKa = 4.08 LAPGAMVTCTASYY1815 pKa = 9.77 TVTQADD1821 pKa = 3.93 INTGSVTNIATAAGSFNNQPVTSNPDD1847 pKa = 3.38 TVTVEE1852 pKa = 4.06 ALQEE1856 pKa = 3.87 PALTLDD1862 pKa = 3.41 KK1863 pKa = 10.69 RR1864 pKa = 11.84 VVSGNPYY1871 pKa = 10.64 DD1872 pKa = 3.65 SVGDD1876 pKa = 3.93 VVQYY1880 pKa = 10.6 TYY1882 pKa = 11.62 DD1883 pKa = 3.42 VTNTGNLTITDD1894 pKa = 5.9 PITVSDD1900 pKa = 4.4 DD1901 pKa = 3.58 KK1902 pKa = 11.08 IATVNCPALPSGGLAPGAMLTCTASYY1928 pKa = 9.84 TITQADD1934 pKa = 3.82 INNGSVTNVATASSGTTQSPPDD1956 pKa = 4.09 TVTVTATQRR1965 pKa = 11.84 PALSLDD1971 pKa = 2.97 KK1972 pKa = 10.66 RR1973 pKa = 11.84 LLSGTTVTAVGDD1985 pKa = 4.18 VIQYY1989 pKa = 10.88 AYY1991 pKa = 10.66 DD1992 pKa = 3.38 VRR1994 pKa = 11.84 NTGNVTITGPITVSDD2009 pKa = 4.08 DD2010 pKa = 3.64 KK2011 pKa = 11.05 IATVTCPAGDD2021 pKa = 4.1 LAPAAMLTCTASYY2034 pKa = 9.83 TVTQADD2040 pKa = 4.31 LDD2042 pKa = 4.2 AGSITNIATASGTFNEE2058 pKa = 4.34 QTVTSNQDD2066 pKa = 3.09 SVTVEE2071 pKa = 4.36 ALHH2074 pKa = 6.21 EE2075 pKa = 4.14 MAVVLTKK2082 pKa = 10.23 IAAKK2086 pKa = 10.32 RR2087 pKa = 11.84 EE2088 pKa = 3.99 VRR2090 pKa = 11.84 VGDD2093 pKa = 3.5 LVRR2096 pKa = 11.84 FTVVARR2102 pKa = 11.84 NVGPGPVVDD2111 pKa = 4.23 ATLIDD2116 pKa = 3.69 TPPRR2120 pKa = 11.84 GFTFVEE2126 pKa = 5.03 GSLTADD2132 pKa = 3.93 DD2133 pKa = 4.69 EE2134 pKa = 4.99 DD2135 pKa = 5.89 DD2136 pKa = 3.81 NVVVSGVDD2144 pKa = 3.7 PIRR2147 pKa = 11.84 ISGVDD2152 pKa = 2.82 IATNGTATFVYY2163 pKa = 9.6 FLRR2166 pKa = 11.84 VGAGVGHH2173 pKa = 6.22 GTHH2176 pKa = 6.85 INRR2179 pKa = 11.84 VIAVDD2184 pKa = 3.75 PTEE2187 pKa = 4.06 HH2188 pKa = 5.81 TVSNEE2193 pKa = 3.11 AAAEE2197 pKa = 4.1 VVIIGDD2203 pKa = 3.69 PMLDD2207 pKa = 3.1 EE2208 pKa = 4.38 SLIVGTVFDD2217 pKa = 5.08 DD2218 pKa = 4.0 RR2219 pKa = 11.84 NGDD2222 pKa = 4.27 GIQQPGEE2229 pKa = 3.78 RR2230 pKa = 11.84 GIPGVRR2236 pKa = 11.84 IGSVEE2241 pKa = 3.96 GLIMEE2246 pKa = 4.67 TDD2248 pKa = 2.98 AFGRR2252 pKa = 11.84 YY2253 pKa = 8.87 HH2254 pKa = 7.17 LVGIDD2259 pKa = 3.19 GGDD2262 pKa = 3.82 FSRR2265 pKa = 11.84 GRR2267 pKa = 11.84 NFILKK2272 pKa = 9.58 VDD2274 pKa = 3.96 ASTLPVGTEE2283 pKa = 3.9 FTTEE2287 pKa = 3.55 NPRR2290 pKa = 11.84 VRR2292 pKa = 11.84 RR2293 pKa = 11.84 ITPGVPVRR2301 pKa = 11.84 FDD2303 pKa = 3.65 FGVKK2307 pKa = 10.25 LPNGEE2312 pKa = 4.21 MKK2314 pKa = 10.61 GGRR2317 pKa = 11.84 QEE2319 pKa = 4.53 LDD2321 pKa = 3.2 AEE2323 pKa = 4.48 LGEE2326 pKa = 4.58 VMFDD2330 pKa = 3.63 PRR2332 pKa = 11.84 SSTLKK2337 pKa = 10.78 AEE2339 pKa = 4.1 YY2340 pKa = 10.44 LPVIDD2345 pKa = 5.05 KK2346 pKa = 10.39 VASKK2350 pKa = 10.37 VRR2352 pKa = 11.84 EE2353 pKa = 4.09 YY2354 pKa = 11.14 NGGRR2358 pKa = 11.84 IVITAKK2364 pKa = 10.81 AEE2366 pKa = 4.06 EE2367 pKa = 4.19 EE2368 pKa = 3.88 ALAYY2372 pKa = 10.31 ARR2374 pKa = 11.84 ARR2376 pKa = 11.84 AVQDD2380 pKa = 3.36 ALRR2383 pKa = 11.84 TKK2385 pKa = 10.76 LEE2387 pKa = 3.91 PALADD2392 pKa = 3.56 RR2393 pKa = 11.84 VAVEE2397 pKa = 4.45 VVADD2401 pKa = 3.71 TSGTMVRR2408 pKa = 11.84 LDD2410 pKa = 3.55 RR2411 pKa = 11.84 GIHH2414 pKa = 6.1 LGTFLFDD2421 pKa = 3.48 TDD2423 pKa = 3.56 KK2424 pKa = 11.54 SVIKK2428 pKa = 9.46 PQYY2431 pKa = 9.14 RR2432 pKa = 11.84 GLIKK2436 pKa = 10.57 EE2437 pKa = 4.18 IADD2440 pKa = 3.52 DD2441 pKa = 4.31 LNRR2444 pKa = 11.84 MGRR2447 pKa = 11.84 GTIGLVGHH2455 pKa = 7.23 ADD2457 pKa = 3.06 MRR2459 pKa = 11.84 ASNEE2463 pKa = 3.67 YY2464 pKa = 10.28 NRR2466 pKa = 11.84 YY2467 pKa = 9.65 LGLRR2471 pKa = 11.84 RR2472 pKa = 11.84 SKK2474 pKa = 11.19 AVFDD2478 pKa = 5.45 AILAQLRR2485 pKa = 11.84 PEE2487 pKa = 3.76 VRR2489 pKa = 11.84 QRR2491 pKa = 11.84 VRR2493 pKa = 11.84 VDD2495 pKa = 2.73 ISDD2498 pKa = 4.45 EE2499 pKa = 3.86 IDD2501 pKa = 3.36 AEE2503 pKa = 4.42 TGVGDD2508 pKa = 3.73 RR2509 pKa = 4.83
Molecular weight: 255.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.77
IPC2_protein 3.986
IPC_protein 4.037
Toseland 3.808
ProMoST 4.202
Dawson 4.037
Bjellqvist 4.19
Wikipedia 3.986
Rodwell 3.859
Grimsley 3.706
Solomon 4.037
Lehninger 3.999
Nozaki 4.139
DTASelect 4.444
Thurlkill 3.859
EMBOSS 3.999
Sillero 4.164
Patrickios 1.659
IPC_peptide 4.037
IPC2_peptide 4.139
IPC2.peptide.svr19 4.026
Protein with the highest isoelectric point:
>tr|A0A0A0F0J3|A0A0A0F0J3_9GAMM 4Fe-4S ferredoxin-type domain-containing protein OS=Lysobacter daejeonensis GH1-9 OX=1385517 GN=N800_10895 PE=4 SV=1
MM1 pKa = 7.62 KK2 pKa = 10.31 ALCHH6 pKa = 6.03 VIAVLVMGLPTPAWSQQAGEE26 pKa = 4.15 LRR28 pKa = 11.84 KK29 pKa = 9.96 CVSPGGAVSFQQQPCAAGSRR49 pKa = 11.84 QTSSRR54 pKa = 11.84 SYY56 pKa = 9.39 VAEE59 pKa = 4.09 PAPTAEE65 pKa = 4.43 QIRR68 pKa = 11.84 ARR70 pKa = 11.84 ATRR73 pKa = 11.84 EE74 pKa = 3.42 QVARR78 pKa = 11.84 AEE80 pKa = 4.22 SAEE83 pKa = 3.93 LSRR86 pKa = 11.84 RR87 pKa = 11.84 AGTSGHH93 pKa = 6.91 LSAPPGRR100 pKa = 11.84 GTLHH104 pKa = 6.39 RR105 pKa = 11.84 VAIAKK110 pKa = 10.09 DD111 pKa = 3.34 DD112 pKa = 4.21 AACQRR117 pKa = 11.84 ARR119 pKa = 11.84 RR120 pKa = 11.84 HH121 pKa = 5.79 RR122 pKa = 11.84 DD123 pKa = 2.89 EE124 pKa = 3.87 TLEE127 pKa = 3.95 RR128 pKa = 11.84 VGLKK132 pKa = 8.79 RR133 pKa = 11.84 TYY135 pKa = 11.23 DD136 pKa = 3.66 LLRR139 pKa = 11.84 ALNDD143 pKa = 3.32 EE144 pKa = 4.39 VARR147 pKa = 11.84 ACRR150 pKa = 3.43
Molecular weight: 16.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.351
IPC2_protein 9.282
IPC_protein 9.999
Toseland 10.379
ProMoST 10.409
Dawson 10.496
Bjellqvist 10.262
Wikipedia 10.716
Rodwell 10.57
Grimsley 10.54
Solomon 10.628
Lehninger 10.599
Nozaki 10.467
DTASelect 10.233
Thurlkill 10.409
EMBOSS 10.789
Sillero 10.452
Patrickios 10.335
IPC_peptide 10.628
IPC2_peptide 9.721
IPC2.peptide.svr19 8.559
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2570
0
2570
830463
41
2509
323.1
35.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.135 ± 0.064
0.825 ± 0.015
5.829 ± 0.04
5.474 ± 0.04
3.376 ± 0.031
8.628 ± 0.046
2.341 ± 0.026
4.044 ± 0.035
2.932 ± 0.036
10.688 ± 0.071
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.266 ± 0.022
2.457 ± 0.035
5.271 ± 0.033
3.543 ± 0.028
7.554 ± 0.054
4.873 ± 0.037
5.082 ± 0.047
7.844 ± 0.043
1.505 ± 0.027
2.332 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here