Amazon lily mild mottle virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Bromoviridae; Anulavirus

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I7HHJ1|I7HHJ1_9BROM RNA-directed RNA polymerase 2a OS=Amazon lily mild mottle virus OX=1195163 GN=2a PE=4 SV=1
MM1 pKa = 7.87ADD3 pKa = 3.84CLVDD7 pKa = 4.77LGIPKK12 pKa = 10.3LKK14 pKa = 10.46LVAALFRR21 pKa = 11.84GEE23 pKa = 4.95DD24 pKa = 3.26ISDD27 pKa = 3.61ISPEE31 pKa = 4.25VVTEE35 pKa = 4.07VCDD38 pKa = 4.17GSFVSYY44 pKa = 10.54HH45 pKa = 6.42CLDD48 pKa = 3.89SSVSPFDD55 pKa = 3.39VCVDD59 pKa = 3.09ILDD62 pKa = 4.4TIKK65 pKa = 11.14NDD67 pKa = 3.09VSVVDD72 pKa = 4.08DD73 pKa = 4.19CVVTTSDD80 pKa = 3.89EE81 pKa = 4.41EE82 pKa = 4.54VTSEE86 pKa = 3.53RR87 pKa = 11.84LEE89 pKa = 4.13RR90 pKa = 11.84VKK92 pKa = 10.37RR93 pKa = 11.84TLDD96 pKa = 4.08LEE98 pKa = 4.56DD99 pKa = 4.52CVVNSCSVCTEE110 pKa = 3.82GNEE113 pKa = 4.01RR114 pKa = 11.84LMEE117 pKa = 4.24SSYY120 pKa = 11.67GNHH123 pKa = 5.25FTLVGEE129 pKa = 4.42EE130 pKa = 4.59FIEE133 pKa = 4.39EE134 pKa = 4.15CPAAAINVASVVPIDD149 pKa = 3.74QQFEE153 pKa = 4.23CDD155 pKa = 3.52LTAPLPVLKK164 pKa = 10.51GRR166 pKa = 11.84SDD168 pKa = 3.28RR169 pKa = 11.84VTLEE173 pKa = 3.88KK174 pKa = 10.93LQTTVDD180 pKa = 4.25AILPTHH186 pKa = 6.57ARR188 pKa = 11.84FDD190 pKa = 4.07DD191 pKa = 3.97TFHH194 pKa = 6.55QAFVEE199 pKa = 4.18NADD202 pKa = 3.29VSLDD206 pKa = 3.54FEE208 pKa = 5.03RR209 pKa = 11.84IRR211 pKa = 11.84LKK213 pKa = 10.67QYY215 pKa = 10.64AGDD218 pKa = 3.82WYY220 pKa = 10.79RR221 pKa = 11.84DD222 pKa = 3.17PDD224 pKa = 3.69NFYY227 pKa = 11.41EE228 pKa = 4.36PVLKK232 pKa = 10.73SGGCSRR238 pKa = 11.84RR239 pKa = 11.84VGSQKK244 pKa = 10.28EE245 pKa = 3.65ALIAIRR251 pKa = 11.84KK252 pKa = 9.04RR253 pKa = 11.84NADD256 pKa = 3.5VPEE259 pKa = 4.17LAGSVNIDD267 pKa = 4.26QVAEE271 pKa = 3.99WAADD275 pKa = 3.26NFMKK279 pKa = 10.74SFVVNVSPLVQVMSKK294 pKa = 9.08MKK296 pKa = 10.45AYY298 pKa = 9.05MEE300 pKa = 4.2KK301 pKa = 9.88WSDD304 pKa = 3.4RR305 pKa = 11.84VDD307 pKa = 3.31PLMVLGEE314 pKa = 4.5TNLQRR319 pKa = 11.84YY320 pKa = 6.28QHH322 pKa = 6.19MIKK325 pKa = 9.6TDD327 pKa = 3.42VKK329 pKa = 10.65PITNDD334 pKa = 2.75GMNLEE339 pKa = 4.26RR340 pKa = 11.84AIPATITFHH349 pKa = 7.85DD350 pKa = 4.74KK351 pKa = 9.86MVCSNFSPWFTALFDD366 pKa = 4.24GLQKK370 pKa = 10.84SLNSRR375 pKa = 11.84VRR377 pKa = 11.84IPSGPISTLEE387 pKa = 3.45MHH389 pKa = 6.68YY390 pKa = 10.87GFKK393 pKa = 10.47NKK395 pKa = 10.36YY396 pKa = 8.25YY397 pKa = 11.06VEE399 pKa = 4.79IDD401 pKa = 3.19LSKK404 pKa = 10.49FDD406 pKa = 3.77KK407 pKa = 11.17SQGEE411 pKa = 3.98LHH413 pKa = 7.1LEE415 pKa = 4.14FQRR418 pKa = 11.84LILLRR423 pKa = 11.84LGLPAHH429 pKa = 6.75LVNWWCEE436 pKa = 3.33LHH438 pKa = 6.31IRR440 pKa = 11.84SFISDD445 pKa = 3.66PTAGVAFQCAYY456 pKa = 9.14QRR458 pKa = 11.84RR459 pKa = 11.84TGDD462 pKa = 2.78AFTFFGNTLVTMAEE476 pKa = 4.43FACCFDD482 pKa = 3.99CSQFEE487 pKa = 4.24MMLFAGDD494 pKa = 3.89DD495 pKa = 3.69SLAVSNSPITGDD507 pKa = 3.17TDD509 pKa = 4.61LFTSLFNMEE518 pKa = 4.88SKK520 pKa = 11.15SMANPVPYY528 pKa = 9.81ICSKK532 pKa = 10.61FLIEE536 pKa = 4.54DD537 pKa = 3.57CFGNSFSVPDD547 pKa = 5.15PIRR550 pKa = 11.84EE551 pKa = 3.88FQRR554 pKa = 11.84LGKK557 pKa = 10.23KK558 pKa = 9.89KK559 pKa = 10.16IQIQPRR565 pKa = 11.84ADD567 pKa = 3.26ALFEE571 pKa = 4.06QYY573 pKa = 10.54QGFRR577 pKa = 11.84DD578 pKa = 3.29RR579 pKa = 11.84MKK581 pKa = 10.75YY582 pKa = 10.0LRR584 pKa = 11.84HH585 pKa = 6.59LDD587 pKa = 3.86DD588 pKa = 6.15FMIDD592 pKa = 3.32QLKK595 pKa = 10.35VYY597 pKa = 10.72FDD599 pKa = 3.24IKK601 pKa = 10.09YY602 pKa = 10.85NKK604 pKa = 10.38GKK606 pKa = 10.44DD607 pKa = 3.79CIDD610 pKa = 4.36DD611 pKa = 3.79FLGGCIYY618 pKa = 10.85YY619 pKa = 10.64ADD621 pKa = 3.97NFKK624 pKa = 10.69HH625 pKa = 5.56FCEE628 pKa = 4.37LFVNHH633 pKa = 5.68SHH635 pKa = 6.96AIEE638 pKa = 4.14VGALVNFVDD647 pKa = 5.31DD648 pKa = 4.67KK649 pKa = 11.42PLGIPSRR656 pKa = 11.84LL657 pKa = 3.38

Molecular weight: 74.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I7H7W8|I7H7W8_9BROM Movement protein OS=Amazon lily mild mottle virus OX=1195163 GN=3a PE=4 SV=1
MM1 pKa = 7.24QNPNRR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84NRR10 pKa = 11.84ANQNRR15 pKa = 11.84MRR17 pKa = 11.84RR18 pKa = 11.84QRR20 pKa = 11.84QAQDD24 pKa = 2.78AAAFRR29 pKa = 11.84ALSSSSRR36 pKa = 11.84LGPSTSALVDD46 pKa = 3.43AGGIPVTPGFVVTSVTLSEE65 pKa = 4.1AFQWSGPGTGAWTLFRR81 pKa = 11.84EE82 pKa = 4.32RR83 pKa = 11.84SLVIPSALPPEE94 pKa = 4.28TRR96 pKa = 11.84LNNVILRR103 pKa = 11.84VTPTPGLASFDD114 pKa = 2.91IWVAVAAAKK123 pKa = 10.52GSTPTAVDD131 pKa = 4.28FDD133 pKa = 4.28ALNVPFLTMRR143 pKa = 11.84EE144 pKa = 4.07TSRR147 pKa = 11.84TRR149 pKa = 11.84HH150 pKa = 4.14VVVPFVNRR158 pKa = 11.84TVADD162 pKa = 4.45LAQQRR167 pKa = 11.84VWLGARR173 pKa = 11.84TGTVTAEE180 pKa = 4.03SLVVGLVRR188 pKa = 11.84VFIEE192 pKa = 4.36HH193 pKa = 6.84RR194 pKa = 11.84PIPQVQIVPVV204 pKa = 3.48

Molecular weight: 22.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

2102

204

945

525.5

58.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.279 ± 0.736

2.854 ± 0.56

6.708 ± 0.908

5.852 ± 0.667

5.186 ± 0.59

5.519 ± 0.489

2.093 ± 0.443

4.567 ± 0.749

6.185 ± 1.301

9.324 ± 0.497

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.236 ± 0.216

3.806 ± 0.197

4.044 ± 0.982

2.95 ± 0.396

5.709 ± 0.809

7.85 ± 1.008

5.233 ± 0.596

8.706 ± 0.695

1.38 ± 0.191

2.521 ± 0.414
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski