Niveispirillum lacus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Azospirillaceae; Niveispirillum

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4265 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A255Z4Z5|A0A255Z4Z5_9PROT DNA-binding protein OS=Niveispirillum lacus OX=1981099 GN=CHU95_04700 PE=4 SV=1
MM1 pKa = 7.29PHH3 pKa = 6.86KK4 pKa = 9.58EE5 pKa = 3.23WFMRR9 pKa = 11.84FSKK12 pKa = 10.52FVASTAIALAMLGGAAAAQAEE33 pKa = 4.39DD34 pKa = 3.68ASFSFSGTAAITSDD48 pKa = 3.76YY49 pKa = 10.87VFRR52 pKa = 11.84GYY54 pKa = 10.5SQTDD58 pKa = 3.21SDD60 pKa = 4.36PAFQAGLTASHH71 pKa = 6.76EE72 pKa = 4.58SGFFVSAWGSNVDD85 pKa = 4.36LGSDD89 pKa = 3.23ADD91 pKa = 3.92IEE93 pKa = 4.29VDD95 pKa = 5.66LIAGYY100 pKa = 8.64TNSVDD105 pKa = 3.16NLTYY109 pKa = 10.32TVGAYY114 pKa = 10.36YY115 pKa = 8.75YY116 pKa = 8.3TYY118 pKa = 10.5PGADD122 pKa = 3.11GSAEE126 pKa = 3.64FDD128 pKa = 3.71YY129 pKa = 11.53FEE131 pKa = 6.29AGLDD135 pKa = 3.33LAYY138 pKa = 10.4AIDD141 pKa = 4.31KK142 pKa = 8.52VTLTGKK148 pKa = 10.38LYY150 pKa = 10.48YY151 pKa = 10.52SPEE154 pKa = 3.95FFGAAGGDD162 pKa = 2.79AWYY165 pKa = 8.88FAGGTSVAVTEE176 pKa = 4.43TVSVYY181 pKa = 10.8GAIGFNEE188 pKa = 4.47LDD190 pKa = 3.78NGTDD194 pKa = 3.54YY195 pKa = 11.4QDD197 pKa = 3.71YY198 pKa = 10.75QGGIAVKK205 pKa = 10.26FDD207 pKa = 3.87PFVLDD212 pKa = 4.99LKK214 pKa = 10.56YY215 pKa = 9.6TDD217 pKa = 4.13TDD219 pKa = 3.67VKK221 pKa = 11.04GAGPLGDD228 pKa = 3.57SRR230 pKa = 11.84FVATLTYY237 pKa = 11.07AFF239 pKa = 4.8

Molecular weight: 25.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A255Z0H4|A0A255Z0H4_9PROT Ferredoxin OS=Niveispirillum lacus OX=1981099 GN=CHU95_10320 PE=4 SV=1
MM1 pKa = 7.49KK2 pKa = 10.41RR3 pKa = 11.84PFQPSKK9 pKa = 9.2IVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.49GFRR19 pKa = 11.84ARR21 pKa = 11.84MATAAGRR28 pKa = 11.84NIINKK33 pKa = 9.09RR34 pKa = 11.84RR35 pKa = 11.84AQGRR39 pKa = 11.84KK40 pKa = 9.19KK41 pKa = 10.65LSAA44 pKa = 3.91

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4265

0

4265

1464700

41

6470

343.4

37.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.494 ± 0.053

0.8 ± 0.013

5.995 ± 0.028

4.971 ± 0.035

3.417 ± 0.024

8.907 ± 0.049

2.031 ± 0.021

4.725 ± 0.024

2.965 ± 0.03

10.72 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.461 ± 0.018

2.635 ± 0.024

5.474 ± 0.038

3.367 ± 0.025

7.218 ± 0.045

5.116 ± 0.04

5.678 ± 0.056

7.449 ± 0.03

1.41 ± 0.016

2.167 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski