Capybara microvirus Cap1_SP_223
Average proteome isoelectric point is 5.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W5P8|A0A4P8W5P8_9VIRU Minor capsid protein OS=Capybara microvirus Cap1_SP_223 OX=2585414 PE=4 SV=1
MM1 pKa = 7.59 ASIFRR6 pKa = 11.84 TGPIVGQTFRR16 pKa = 11.84 RR17 pKa = 11.84 FYY19 pKa = 10.53 PKK21 pKa = 10.58 YY22 pKa = 10.14 GLGKK26 pKa = 10.17 DD27 pKa = 3.73 GYY29 pKa = 10.46 LVEE32 pKa = 4.61 LPEE35 pKa = 5.39 VIDD38 pKa = 3.65 NQQIIDD44 pKa = 3.93 DD45 pKa = 4.97 AYY47 pKa = 10.99 DD48 pKa = 3.97 DD49 pKa = 4.24 SLNVLLDD56 pKa = 3.44 KK57 pKa = 11.12 YY58 pKa = 10.45 IYY60 pKa = 10.56 EE61 pKa = 4.68 PVLEE65 pKa = 4.59 DD66 pKa = 3.69 QNDD69 pKa = 3.52 YY70 pKa = 11.47 SDD72 pKa = 5.8 DD73 pKa = 4.3 YY74 pKa = 11.45 IDD76 pKa = 3.73 YY77 pKa = 10.84 RR78 pKa = 11.84 RR79 pKa = 11.84 DD80 pKa = 3.3 ANALEE85 pKa = 4.56 SFLKK89 pKa = 10.5 ADD91 pKa = 3.69 EE92 pKa = 4.55 LAAEE96 pKa = 4.26 LRR98 pKa = 11.84 VAYY101 pKa = 9.55 GMPRR105 pKa = 11.84 ASISDD110 pKa = 3.54 LRR112 pKa = 11.84 KK113 pKa = 10.41 EE114 pKa = 3.57 MDD116 pKa = 3.2 KK117 pKa = 10.99 RR118 pKa = 11.84 FSKK121 pKa = 11.16 YY122 pKa = 8.18 EE123 pKa = 4.14 SKK125 pKa = 10.84 EE126 pKa = 3.69 VTFDD130 pKa = 3.21 EE131 pKa = 4.77 KK132 pKa = 11.29 AQNEE136 pKa = 4.35 TVEE139 pKa = 4.19 VGKK142 pKa = 10.53 QEE144 pKa = 4.24 VVV146 pKa = 2.95
Molecular weight: 16.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.45
IPC2_protein 4.393
IPC_protein 4.329
Toseland 4.151
ProMoST 4.457
Dawson 4.291
Bjellqvist 4.444
Wikipedia 4.19
Rodwell 4.164
Grimsley 4.05
Solomon 4.291
Lehninger 4.24
Nozaki 4.406
DTASelect 4.596
Thurlkill 4.164
EMBOSS 4.202
Sillero 4.444
Patrickios 3.795
IPC_peptide 4.291
IPC2_peptide 4.431
IPC2.peptide.svr19 4.386
Protein with the highest isoelectric point:
>tr|A0A4P8W874|A0A4P8W874_9VIRU Replication initiator protein OS=Capybara microvirus Cap1_SP_223 OX=2585414 PE=4 SV=1
MM1 pKa = 7.49 KK2 pKa = 10.11 CLSPFFTSLGPVPCGRR18 pKa = 11.84 CINCQINRR26 pKa = 11.84 SQQWSIRR33 pKa = 11.84 CLDD36 pKa = 3.46 EE37 pKa = 5.19 FKK39 pKa = 10.12 ITGIGFFLTLTFKK52 pKa = 10.27 KK53 pKa = 9.19 TDD55 pKa = 2.94 GKK57 pKa = 10.7 LRR59 pKa = 11.84 RR60 pKa = 11.84 KK61 pKa = 9.29 PLQDD65 pKa = 3.06 FLKK68 pKa = 10.35 RR69 pKa = 11.84 LRR71 pKa = 11.84 KK72 pKa = 9.65 EE73 pKa = 3.45 ISPVKK78 pKa = 9.93 IRR80 pKa = 11.84 YY81 pKa = 7.7 FGCGEE86 pKa = 4.06 YY87 pKa = 10.56 GSKK90 pKa = 10.44 GLRR93 pKa = 11.84 PHH95 pKa = 5.7 YY96 pKa = 10.11 HH97 pKa = 6.37 LLVFGWRR104 pKa = 11.84 PDD106 pKa = 3.52 DD107 pKa = 3.71 LVSFRR112 pKa = 11.84 VDD114 pKa = 2.73 RR115 pKa = 11.84 KK116 pKa = 10.11 GQILYY121 pKa = 10.26 RR122 pKa = 11.84 SALIEE127 pKa = 4.73 RR128 pKa = 11.84 IWHH131 pKa = 5.0 SHH133 pKa = 4.34 TDD135 pKa = 3.16 NSYY138 pKa = 8.62 VAGFISIGDD147 pKa = 3.72 INEE150 pKa = 3.99 VTCRR154 pKa = 11.84 YY155 pKa = 9.36 ASKK158 pKa = 10.44 YY159 pKa = 9.49 CSKK162 pKa = 10.72 LGSFDD167 pKa = 3.8 SEE169 pKa = 4.45 YY170 pKa = 10.54 PPFSAMSTHH179 pKa = 6.74 PGIGASSVSLARR191 pKa = 11.84 CWQGTIVYY199 pKa = 9.06 QNKK202 pKa = 8.78 VVPAPRR208 pKa = 11.84 YY209 pKa = 10.17 YY210 pKa = 9.09 ITCLSRR216 pKa = 11.84 QGVSVDD222 pKa = 4.22 GILADD227 pKa = 3.83 RR228 pKa = 11.84 RR229 pKa = 11.84 SRR231 pKa = 11.84 SCLLNGDD238 pKa = 4.28 YY239 pKa = 10.64 PVLDD243 pKa = 3.63 YY244 pKa = 11.9 NEE246 pKa = 4.17 IEE248 pKa = 4.34 RR249 pKa = 11.84 ACFEE253 pKa = 3.63 ARR255 pKa = 11.84 QRR257 pKa = 11.84 LEE259 pKa = 3.8 RR260 pKa = 11.84 KK261 pKa = 9.01 IPKK264 pKa = 10.05 NGG266 pKa = 3.18
Molecular weight: 30.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.908
IPC2_protein 8.902
IPC_protein 8.843
Toseland 9.282
ProMoST 9.253
Dawson 9.648
Bjellqvist 9.545
Wikipedia 9.838
Rodwell 9.809
Grimsley 9.706
Solomon 9.692
Lehninger 9.648
Nozaki 9.677
DTASelect 9.443
Thurlkill 9.516
EMBOSS 9.78
Sillero 9.692
Patrickios 4.584
IPC_peptide 9.692
IPC2_peptide 8.668
IPC2.peptide.svr19 7.83
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1292
75
527
258.4
28.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.43 ± 2.419
1.935 ± 0.878
6.192 ± 0.909
4.876 ± 0.983
5.031 ± 0.47
6.579 ± 0.852
1.625 ± 0.434
5.263 ± 0.694
3.87 ± 0.793
6.966 ± 0.744
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.858 ± 0.485
5.341 ± 1.018
4.412 ± 0.914
4.954 ± 0.654
5.805 ± 0.946
10.372 ± 1.322
4.954 ± 0.598
6.502 ± 0.751
1.471 ± 0.244
4.567 ± 0.515
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here