Microbacterium phage Min1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Minunavirus; Microbacterium virus Min1

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A6N234|A6N234_9CAUD Uncharacterized protein OS=Microbacterium phage Min1 OX=446529 PE=4 SV=1
MM1 pKa = 7.78TDD3 pKa = 3.71LSSPEE8 pKa = 3.88YY9 pKa = 10.72GAATCASCGVVLRR22 pKa = 11.84DD23 pKa = 3.72DD24 pKa = 4.21EE25 pKa = 5.53GGLRR29 pKa = 11.84CPSCGTIILAPVVVQPVFDD48 pKa = 5.15GPDD51 pKa = 3.14IDD53 pKa = 4.4DD54 pKa = 3.4WRR56 pKa = 5.79

Molecular weight: 5.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A6N1W0|A6N1W0_9CAUD Probable c1 repressor OS=Microbacterium phage Min1 OX=446529 PE=4 SV=1
MM1 pKa = 7.3YY2 pKa = 10.83GFFTWMQEE10 pKa = 3.85EE11 pKa = 4.86EE12 pKa = 4.24FRR14 pKa = 11.84LDD16 pKa = 3.13NPAARR21 pKa = 11.84LPKK24 pKa = 9.68VRR26 pKa = 11.84VPKK29 pKa = 10.54VEE31 pKa = 4.33PDD33 pKa = 3.34PVTTEE38 pKa = 4.49DD39 pKa = 3.64IEE41 pKa = 4.71LVLHH45 pKa = 5.78SGIYY49 pKa = 9.63RR50 pKa = 11.84RR51 pKa = 11.84TRR53 pKa = 11.84MWVLLYY59 pKa = 10.32AYY61 pKa = 9.91QGMRR65 pKa = 11.84AAEE68 pKa = 3.96IAAVSGSSIDD78 pKa = 3.13WKK80 pKa = 10.84ARR82 pKa = 11.84RR83 pKa = 11.84ILSIEE88 pKa = 3.83AKK90 pKa = 10.01GGKK93 pKa = 7.85EE94 pKa = 3.69VWRR97 pKa = 11.84PLHH100 pKa = 5.35PVVWEE105 pKa = 4.03QLQTWHH111 pKa = 5.03TTGWLFPSPTRR122 pKa = 11.84KK123 pKa = 10.06GEE125 pKa = 4.01HH126 pKa = 3.99VTAANVSNVLSKK138 pKa = 10.53AFKK141 pKa = 9.94RR142 pKa = 11.84AGVAHH147 pKa = 7.48RR148 pKa = 11.84PHH150 pKa = 7.42QMRR153 pKa = 11.84AWFATEE159 pKa = 4.37MIAAGTPTIVVAAAMRR175 pKa = 11.84HH176 pKa = 5.8SDD178 pKa = 3.41TQSVEE183 pKa = 3.67KK184 pKa = 10.26YY185 pKa = 9.92VRR187 pKa = 11.84VRR189 pKa = 11.84DD190 pKa = 4.1DD191 pKa = 4.39KK192 pKa = 11.19IAEE195 pKa = 4.21AMLALPRR202 pKa = 11.84FQIPSRR208 pKa = 11.84SGRR211 pKa = 11.84RR212 pKa = 11.84VALAKK217 pKa = 9.68PASVAQGIEE226 pKa = 3.85QRR228 pKa = 11.84PSNSRR233 pKa = 11.84VDD235 pKa = 3.54GSNPSGGTRR244 pKa = 11.84RR245 pKa = 11.84TARR248 pKa = 11.84QQQGDD253 pKa = 3.46RR254 pKa = 11.84AAA256 pKa = 4.18

Molecular weight: 28.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

77

0

77

14028

34

1010

182.2

19.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.459 ± 0.547

0.834 ± 0.132

6.352 ± 0.36

6.309 ± 0.344

2.246 ± 0.166

8.975 ± 0.454

2.103 ± 0.23

3.999 ± 0.215

2.395 ± 0.202

8.533 ± 0.373

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.775 ± 0.107

1.932 ± 0.146

5.66 ± 0.301

3.058 ± 0.176

8.576 ± 0.449

5.375 ± 0.361

6.337 ± 0.308

8.312 ± 0.326

1.925 ± 0.176

1.846 ± 0.177
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski