Staphylococcus phage 6ec

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Sextaecvirus; Staphylococcus virus Sextaec

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 142 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A060AES8|A0A060AES8_9CAUD Uncharacterized protein OS=Staphylococcus phage 6ec OX=1500386 GN=PHAGE6E_4 PE=4 SV=1
MM1 pKa = 7.41NKK3 pKa = 10.08SKK5 pKa = 9.33TLIYY9 pKa = 9.9IDD11 pKa = 4.5SLLLSYY17 pKa = 9.8EE18 pKa = 4.12IKK20 pKa = 10.41QEE22 pKa = 4.44SIGADD27 pKa = 2.93IDD29 pKa = 3.69IVDD32 pKa = 4.36VIGTTEE38 pKa = 3.96VNNIIYY44 pKa = 10.24LALEE48 pKa = 3.99VEE50 pKa = 4.51YY51 pKa = 11.29NDD53 pKa = 3.49YY54 pKa = 11.22CYY56 pKa = 9.77LTHH59 pKa = 7.35VEE61 pKa = 4.61LVNMWAYY68 pKa = 10.21PNDD71 pKa = 4.25DD72 pKa = 4.2DD73 pKa = 4.41KK74 pKa = 12.04QEE76 pKa = 4.1IKK78 pKa = 10.94EE79 pKa = 4.06EE80 pKa = 3.91LLNNIFKK87 pKa = 10.74DD88 pKa = 3.21IAFQSSSINVGDD100 pKa = 3.74VVRR103 pKa = 11.84YY104 pKa = 8.47TDD106 pKa = 5.44DD107 pKa = 3.97IYY109 pKa = 11.09IKK111 pKa = 10.43NIKK114 pKa = 9.53NDD116 pKa = 3.55YY117 pKa = 10.64FFLSDD122 pKa = 3.68NEE124 pKa = 4.4TPKK127 pKa = 10.12MDD129 pKa = 3.23VNYY132 pKa = 9.95IVSDD136 pKa = 3.28LHH138 pKa = 7.86YY139 pKa = 11.17DD140 pKa = 5.0GIDD143 pKa = 3.38EE144 pKa = 4.15QNNLIVLYY152 pKa = 9.96QLEE155 pKa = 4.61KK156 pKa = 10.72DD157 pKa = 3.9DD158 pKa = 4.9EE159 pKa = 4.52YY160 pKa = 11.83LGILTNEE167 pKa = 3.93FEE169 pKa = 4.18IEE171 pKa = 4.25KK172 pKa = 10.62VSEE175 pKa = 4.0

Molecular weight: 20.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A060AKE0|A0A060AKE0_9CAUD Endodeoxyribonuclease OS=Staphylococcus phage 6ec OX=1500386 GN=PHAGE6E_21 PE=4 SV=1
MM1 pKa = 7.51FIIGLVFIICSFIIIGQFIIMPILIFLGIVHH32 pKa = 6.79SGKK35 pKa = 9.77QEE37 pKa = 3.96LQQEE41 pKa = 4.44VANNKK46 pKa = 9.12FSKK49 pKa = 10.67EE50 pKa = 3.68NGKK53 pKa = 10.33RR54 pKa = 11.84SLEE57 pKa = 3.98LLKK60 pKa = 10.92SEE62 pKa = 4.65LNRR65 pKa = 11.84VAGSNTILYY74 pKa = 9.98KK75 pKa = 9.71RR76 pKa = 11.84GKK78 pKa = 10.35YY79 pKa = 9.53VVTLKK84 pKa = 10.99NPTDD88 pKa = 3.84NNIKK92 pKa = 10.27AARR95 pKa = 11.84KK96 pKa = 6.53QFKK99 pKa = 9.44MRR101 pKa = 11.84GITNYY106 pKa = 9.77KK107 pKa = 9.47VKK109 pKa = 10.89

Molecular weight: 12.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

142

0

142

27663

32

1960

194.8

22.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.356 ± 0.304

0.756 ± 0.093

6.98 ± 0.267

7.949 ± 0.345

4.157 ± 0.161

5.675 ± 0.461

1.627 ± 0.092

8.116 ± 0.275

9.796 ± 0.311

8.163 ± 0.253

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.628 ± 0.118

7.241 ± 0.211

2.176 ± 0.095

3.206 ± 0.15

3.503 ± 0.131

6.153 ± 0.192

5.462 ± 0.148

6.008 ± 0.168

1.052 ± 0.193

4.996 ± 0.29
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski