Furfurilactobacillus rossiae DSM 15814
Average proteome isoelectric point is 6.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2635 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R1RDU9|A0A0R1RDU9_9LACO Maltose O-acetyltransferase OS=Furfurilactobacillus rossiae DSM 15814 OX=1114972 GN=FD35_GL002501 PE=3 SV=1
MM1 pKa = 7.64 ASDD4 pKa = 3.73 AHH6 pKa = 5.35 QVPVSFNDD14 pKa = 3.5 TTLTDD19 pKa = 3.97 LKK21 pKa = 10.6 AYY23 pKa = 10.73 CEE25 pKa = 4.27 FFSVDD30 pKa = 3.13 QDD32 pKa = 3.61 QLINTVLCHH41 pKa = 5.89 FLEE44 pKa = 4.38 NHH46 pKa = 6.43 EE47 pKa = 4.39 SADD50 pKa = 4.19 LNKK53 pKa = 10.17 LAQGYY58 pKa = 8.86 LAMGQLNEE66 pKa = 4.49 EE67 pKa = 4.26 IADD70 pKa = 3.68 EE71 pKa = 4.72 FSASEE76 pKa = 4.27 AEE78 pKa = 4.83 ASRR81 pKa = 11.84 LDD83 pKa = 3.47 QQ84 pKa = 4.64
Molecular weight: 9.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.785
IPC2_protein 3.986
IPC_protein 3.897
Toseland 3.719
ProMoST 4.062
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.808
Rodwell 3.745
Grimsley 3.63
Solomon 3.872
Lehninger 3.821
Nozaki 4.012
DTASelect 4.19
Thurlkill 3.77
EMBOSS 3.821
Sillero 4.024
Patrickios 1.901
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.91
Protein with the highest isoelectric point:
>tr|A0A0R1RL82|A0A0R1RL82_9LACO Glutathione reductase OS=Furfurilactobacillus rossiae DSM 15814 OX=1114972 GN=FD35_GL002479 PE=3 SV=1
MM1 pKa = 7.35 SQTHH5 pKa = 4.9 WQRR8 pKa = 11.84 VRR10 pKa = 11.84 NLVAIICFIIFLILLAGVVTDD31 pKa = 5.56 ASWLHH36 pKa = 5.83 QLDD39 pKa = 3.32 QFGTHH44 pKa = 5.88 IVRR47 pKa = 11.84 HH48 pKa = 5.68 PVTEE52 pKa = 3.69 EE53 pKa = 3.69 RR54 pKa = 11.84 TWFFRR59 pKa = 11.84 NITRR63 pKa = 11.84 LGNVKK68 pKa = 8.72 FTMIVMVVFSLGLWWRR84 pKa = 11.84 KK85 pKa = 9.39 KK86 pKa = 8.67 YY87 pKa = 10.45 QGAVFFFVNVGVFALAVNTILKK109 pKa = 10.4 SLVEE113 pKa = 4.2 RR114 pKa = 11.84 PRR116 pKa = 11.84 PIITHH121 pKa = 6.34 LVAAGGFSFPSGHH134 pKa = 6.09 SMNVTLMYY142 pKa = 10.58 GSLIILCNLYY152 pKa = 10.42 VDD154 pKa = 4.48 RR155 pKa = 11.84 LHH157 pKa = 7.2 LRR159 pKa = 11.84 AAFNTLLTLLIITICLSRR177 pKa = 11.84 VYY179 pKa = 10.52 LGVHH183 pKa = 6.31 FPSDD187 pKa = 3.74 VLAGSALAWSEE198 pKa = 4.02 LLMSRR203 pKa = 11.84 YY204 pKa = 10.01 LFFKK208 pKa = 7.76 TTSIHH213 pKa = 6.64 AKK215 pKa = 9.69 RR216 pKa = 3.91
Molecular weight: 24.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.345
IPC2_protein 9.736
IPC_protein 10.526
Toseland 10.496
ProMoST 10.423
Dawson 10.657
Bjellqvist 10.379
Wikipedia 10.862
Rodwell 10.862
Grimsley 10.716
Solomon 10.73
Lehninger 10.701
Nozaki 10.511
DTASelect 10.379
Thurlkill 10.526
EMBOSS 10.906
Sillero 10.584
Patrickios 10.57
IPC_peptide 10.73
IPC2_peptide 9.531
IPC2.peptide.svr19 8.504
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2635
0
2635
804405
39
3552
305.3
33.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.697 ± 0.1
0.407 ± 0.01
6.106 ± 0.052
4.904 ± 0.053
4.224 ± 0.048
6.769 ± 0.042
2.403 ± 0.032
6.471 ± 0.057
5.399 ± 0.046
9.564 ± 0.08
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.722 ± 0.029
4.816 ± 0.045
3.681 ± 0.027
4.716 ± 0.038
4.247 ± 0.054
6.259 ± 0.078
6.842 ± 0.098
7.404 ± 0.041
1.132 ± 0.02
3.237 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here