Farfantepenaeus duorarum circovirus
Average proteome isoelectric point is 7.78
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S5U8K1|S5U8K1_9CIRC Capsid protein OS=Farfantepenaeus duorarum circovirus OX=1380894 PE=4 SV=1
MM1 pKa = 7.21 SGSRR5 pKa = 11.84 RR6 pKa = 11.84 WCFTVFNYY14 pKa = 10.06 NHH16 pKa = 6.48 ATVDD20 pKa = 3.4 DD21 pKa = 5.07 FIRR24 pKa = 11.84 AEE26 pKa = 3.77 RR27 pKa = 11.84 HH28 pKa = 5.09 DD29 pKa = 3.88 CVRR32 pKa = 11.84 YY33 pKa = 9.49 VVWQCEE39 pKa = 4.09 KK40 pKa = 10.49 CPTSGRR46 pKa = 11.84 SHH48 pKa = 5.56 IQGYY52 pKa = 9.08 VEE54 pKa = 3.71 LHH56 pKa = 5.4 RR57 pKa = 11.84 PIRR60 pKa = 11.84 LRR62 pKa = 11.84 QLKK65 pKa = 10.27 QILDD69 pKa = 3.65 DD70 pKa = 3.94 TGAHH74 pKa = 6.24 CEE76 pKa = 3.91 AAKK79 pKa = 10.43 GPRR82 pKa = 11.84 DD83 pKa = 3.3 ACRR86 pKa = 11.84 EE87 pKa = 3.97 YY88 pKa = 10.77 CQKK91 pKa = 10.85 EE92 pKa = 3.99 EE93 pKa = 4.49 SRR95 pKa = 11.84 CCGWWEE101 pKa = 3.84 SGEE104 pKa = 4.18 WIGGQGSRR112 pKa = 11.84 SDD114 pKa = 3.75 LSAVHH119 pKa = 6.84 AAIQDD124 pKa = 3.86 GASLRR129 pKa = 11.84 EE130 pKa = 3.72 ISNAHH135 pKa = 6.12 FDD137 pKa = 3.79 AFVKK141 pKa = 9.96 YY142 pKa = 10.45 SRR144 pKa = 11.84 GIKK147 pKa = 9.94 EE148 pKa = 4.4 YY149 pKa = 10.47 ILLNADD155 pKa = 2.95 RR156 pKa = 11.84 RR157 pKa = 11.84 MWPMEE162 pKa = 3.64 VWVLYY167 pKa = 11.08 GDD169 pKa = 3.89 TEE171 pKa = 4.44 TGKK174 pKa = 8.17 TRR176 pKa = 11.84 AVYY179 pKa = 10.43 EE180 pKa = 4.15 MAPDD184 pKa = 4.21 VYY186 pKa = 11.19 SLAPSHH192 pKa = 6.8 SNGVGWWQMYY202 pKa = 9.24 EE203 pKa = 3.72 GHH205 pKa = 7.37 ADD207 pKa = 3.36 VLLDD211 pKa = 5.8 DD212 pKa = 4.74 FYY214 pKa = 11.96 GWLRR218 pKa = 11.84 YY219 pKa = 9.92 SFLLQLLDD227 pKa = 4.18 RR228 pKa = 11.84 YY229 pKa = 9.19 PLRR232 pKa = 11.84 VEE234 pKa = 4.21 SKK236 pKa = 9.25 GGVVQFVGRR245 pKa = 11.84 RR246 pKa = 11.84 IFITSNAHH254 pKa = 5.41 PNQWYY259 pKa = 9.18 PNVKK263 pKa = 8.82 DD264 pKa = 3.36 TAAMFRR270 pKa = 11.84 RR271 pKa = 11.84 FTRR274 pKa = 11.84 IYY276 pKa = 9.8 HH277 pKa = 6.05 CDD279 pKa = 3.01 KK280 pKa = 10.92 SRR282 pKa = 11.84 FWRR285 pKa = 11.84 EE286 pKa = 3.31 KK287 pKa = 10.31
Molecular weight: 33.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.304
IPC2_protein 7.512
IPC_protein 7.38
Toseland 7.015
ProMoST 8.039
Dawson 8.039
Bjellqvist 8.478
Wikipedia 7.951
Rodwell 8.039
Grimsley 7.073
Solomon 8.097
Lehninger 8.126
Nozaki 8.668
DTASelect 8.185
Thurlkill 8.214
EMBOSS 8.258
Sillero 8.565
Patrickios 4.05
IPC_peptide 8.097
IPC2_peptide 7.79
IPC2.peptide.svr19 7.668
Protein with the highest isoelectric point:
>tr|S5U8K1|S5U8K1_9CIRC Capsid protein OS=Farfantepenaeus duorarum circovirus OX=1380894 PE=4 SV=1
MM1 pKa = 7.48 NLMLNRR7 pKa = 11.84 PRR9 pKa = 11.84 LPDD12 pKa = 3.32 SSQTKK17 pKa = 9.77 GPYY20 pKa = 10.1 GYY22 pKa = 10.87 AVWANLYY29 pKa = 8.65 KK30 pKa = 9.85 TVRR33 pKa = 11.84 TRR35 pKa = 11.84 GVKK38 pKa = 9.69 IEE40 pKa = 3.74 LWSRR44 pKa = 11.84 NRR46 pKa = 11.84 SAQPTGSGATFVGFQGSEE64 pKa = 4.01 DD65 pKa = 4.94 PIPVSDD71 pKa = 3.9 TDD73 pKa = 3.88 HH74 pKa = 6.63 MEE76 pKa = 4.02 HH77 pKa = 6.99 LLARR81 pKa = 11.84 RR82 pKa = 11.84 NVIVKK87 pKa = 10.67 SNGVPAAGKK96 pKa = 10.15 NGSTYY101 pKa = 10.11 IKK103 pKa = 10.31 AYY105 pKa = 10.42 RR106 pKa = 11.84 KK107 pKa = 7.84 LTNIEE112 pKa = 3.62 GHH114 pKa = 5.35 QIEE117 pKa = 4.91 RR118 pKa = 11.84 DD119 pKa = 3.11 SDD121 pKa = 3.59 YY122 pKa = 11.04 WADD125 pKa = 3.85 TQQGDD130 pKa = 3.33 PAKK133 pKa = 10.18 FVKK136 pKa = 10.36 AYY138 pKa = 10.72 VGVTSAGNVDD148 pKa = 3.54 TDD150 pKa = 3.46 TSFDD154 pKa = 3.41 LTVRR158 pKa = 11.84 LTFYY162 pKa = 11.19 VDD164 pKa = 3.37 FWGIRR169 pKa = 11.84 DD170 pKa = 3.77 VPSNTLVDD178 pKa = 4.78 FAAA181 pKa = 4.78
Molecular weight: 20.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.259
IPC2_protein 8.273
IPC_protein 8.448
Toseland 8.858
ProMoST 8.653
Dawson 9.107
Bjellqvist 8.799
Wikipedia 9.326
Rodwell 9.209
Grimsley 9.194
Solomon 9.311
Lehninger 9.253
Nozaki 8.697
DTASelect 8.858
Thurlkill 8.902
EMBOSS 9.224
Sillero 9.019
Patrickios 4.571
IPC_peptide 9.297
IPC2_peptide 7.541
IPC2.peptide.svr19 7.637
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
468
181
287
234.0
26.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.265 ± 0.275
2.137 ± 1.251
6.838 ± 0.525
4.701 ± 1.134
4.06 ± 0.113
7.692 ± 0.348
2.991 ± 0.781
4.06 ± 0.113
4.274 ± 0.409
6.197 ± 0.07
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.923 ± 0.155
3.846 ± 0.982
3.846 ± 0.659
3.632 ± 0.186
8.547 ± 1.122
6.838 ± 0.202
5.342 ± 1.724
7.479 ± 0.797
3.419 ± 0.707
4.915 ± 0.289
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here