Planaria asexual strain-specific virus-like element type 1
Average proteome isoelectric point is 7.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q91S74|Q91S74_9VIRU ORF1S OS=Planaria asexual strain-specific virus-like element type 1 OX=159252 GN=ORF1S PE=4 SV=1
MM1 pKa = 7.39 NLIQAISDD9 pKa = 4.01 DD10 pKa = 4.0 EE11 pKa = 4.28 LQLFIINAMDD21 pKa = 4.45 HH22 pKa = 7.01 IIRR25 pKa = 11.84 HH26 pKa = 5.25 SVFEE30 pKa = 4.39 YY31 pKa = 10.74 NDD33 pKa = 2.98 SHH35 pKa = 7.92 RR36 pKa = 11.84 NVIEE40 pKa = 5.1 KK41 pKa = 8.96 MLQYY45 pKa = 11.22 AGVKK49 pKa = 9.9 KK50 pKa = 10.57 EE51 pKa = 3.92 NEE53 pKa = 4.14 DD54 pKa = 3.6 YY55 pKa = 10.42 EE56 pKa = 4.9 FKK58 pKa = 10.66 YY59 pKa = 9.62 KK60 pKa = 10.09 TMAIISILEE69 pKa = 3.94 MQAGKK74 pKa = 8.87 TNVILMVGKK83 pKa = 8.83 PNCGKK88 pKa = 10.56 SLFCNVLCAGLKK100 pKa = 9.31 TGAAHH105 pKa = 7.04 SMNSRR110 pKa = 11.84 SNFWLQDD117 pKa = 3.58 LQGKK121 pKa = 9.56 DD122 pKa = 2.58 IYY124 pKa = 11.32 VFEE127 pKa = 4.58 EE128 pKa = 4.32 FSMDD132 pKa = 3.45 VFNANYY138 pKa = 9.67 FKK140 pKa = 11.16 ALLEE144 pKa = 4.33 GSMHH148 pKa = 7.0 VMVEE152 pKa = 4.23 KK153 pKa = 10.96 KK154 pKa = 10.56 NMPSVHH160 pKa = 6.46 LEE162 pKa = 3.65 RR163 pKa = 11.84 RR164 pKa = 11.84 PVITTGNEE172 pKa = 4.17 FPWKK176 pKa = 9.69 LVSSTAEE183 pKa = 3.75 AFRR186 pKa = 11.84 EE187 pKa = 3.67 RR188 pKa = 11.84 SLIILTDD195 pKa = 3.41 SQIPFSVSADD205 pKa = 3.49 PNKK208 pKa = 10.78 RR209 pKa = 11.84 PDD211 pKa = 4.08 LLLNCKK217 pKa = 9.84 NLLYY221 pKa = 10.62 EE222 pKa = 4.83 SIKK225 pKa = 11.04 NCC227 pKa = 4.49
Molecular weight: 25.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.966
IPC2_protein 5.957
IPC_protein 6.033
Toseland 6.338
ProMoST 6.415
Dawson 6.338
Bjellqvist 6.313
Wikipedia 6.351
Rodwell 6.326
Grimsley 6.453
Solomon 6.338
Lehninger 6.338
Nozaki 6.605
DTASelect 6.781
Thurlkill 6.81
EMBOSS 6.781
Sillero 6.722
Patrickios 4.202
IPC_peptide 6.364
IPC2_peptide 6.62
IPC2.peptide.svr19 6.63
Protein with the highest isoelectric point:
>tr|Q91S72|Q91S72_9VIRU ORF1L OS=Planaria asexual strain-specific virus-like element type 1 OX=159252 GN=ORF1L PE=4 SV=1
MM1 pKa = 7.83 GIRR4 pKa = 11.84 RR5 pKa = 11.84 KK6 pKa = 10.08 KK7 pKa = 7.55 VTKK10 pKa = 9.48 TVTPKK15 pKa = 9.82 RR16 pKa = 11.84 TTQHH20 pKa = 5.48 IQNPAYY26 pKa = 10.28 SGTPGGPQAASTPLRR41 pKa = 11.84 NTPTRR46 pKa = 11.84 TPPTKK51 pKa = 8.5 TTPIRR56 pKa = 11.84 NRR58 pKa = 11.84 LRR60 pKa = 11.84 SRR62 pKa = 11.84 VVGRR66 pKa = 11.84 NGQAVDD72 pKa = 4.31 RR73 pKa = 11.84 PLLSYY78 pKa = 10.32 RR79 pKa = 11.84 GRR81 pKa = 11.84 GANFGIMHH89 pKa = 7.18 NSPTNPRR96 pKa = 11.84 NIMRR100 pKa = 11.84 MQPRR104 pKa = 11.84 VMVEE108 pKa = 3.85 NILNLSNYY116 pKa = 9.69 RR117 pKa = 11.84 EE118 pKa = 4.25 TSFGTPTQTPRR129 pKa = 11.84 QTPQVTPRR137 pKa = 11.84 NSPTQPPIQPPNPEE151 pKa = 4.06 IPRR154 pKa = 11.84 PSQPIINKK162 pKa = 8.12 NTDD165 pKa = 2.83 GTGPGPSGVQYY176 pKa = 10.71 TKK178 pKa = 11.29 DD179 pKa = 3.23 NTILDD184 pKa = 3.89 EE185 pKa = 4.84 SSILKK190 pKa = 10.12 DD191 pKa = 3.18 KK192 pKa = 10.43 KK193 pKa = 10.31 RR194 pKa = 11.84 NYY196 pKa = 9.26 NLRR199 pKa = 11.84 RR200 pKa = 11.84 RR201 pKa = 11.84 LISKK205 pKa = 8.42 FKK207 pKa = 11.14 NNNNKK212 pKa = 10.13 NISTKK217 pKa = 10.89 SNIPLIPMTSFKK229 pKa = 10.86 NKK231 pKa = 9.26 PQILPKK237 pKa = 9.45 PQPKK241 pKa = 10.42 GILKK245 pKa = 10.13 PNIIPKK251 pKa = 9.96 IKK253 pKa = 9.35 PAYY256 pKa = 7.94 PPKK259 pKa = 10.63 HH260 pKa = 6.48 FITNKK265 pKa = 9.37 PPPIRR270 pKa = 11.84 YY271 pKa = 8.9 NPSSKK276 pKa = 10.22 ILGWGTDD283 pKa = 2.97 KK284 pKa = 11.14 GVKK287 pKa = 9.2 IKK289 pKa = 10.9 SGVGGILGIGKK300 pKa = 7.74 EE301 pKa = 4.34 TTKK304 pKa = 10.62 LGHH307 pKa = 6.67 FIKK310 pKa = 10.05 GTAKK314 pKa = 10.19 AGLIIGLGLTLGQVLYY330 pKa = 9.15 ATGQYY335 pKa = 8.95 FQQARR340 pKa = 11.84 IDD342 pKa = 4.28 KK343 pKa = 9.36 MAFDD347 pKa = 6.18 DD348 pKa = 3.49 IHH350 pKa = 8.12 NEE352 pKa = 3.47 IKK354 pKa = 9.88 TVFPRR359 pKa = 11.84 LVLMNRR365 pKa = 11.84 YY366 pKa = 8.45 VKK368 pKa = 10.31 QFQNFKK374 pKa = 10.48 FKK376 pKa = 10.94 HH377 pKa = 5.75 NPFKK381 pKa = 10.67 DD382 pKa = 4.01 TIPKK386 pKa = 9.95 AIQRR390 pKa = 11.84 LADD393 pKa = 3.2 QALNIEE399 pKa = 4.38 RR400 pKa = 11.84 LKK402 pKa = 10.58 IAHH405 pKa = 6.55 KK406 pKa = 10.44 NSYY409 pKa = 9.71 FGVRR413 pKa = 11.84 IPQEE417 pKa = 4.16 TKK419 pKa = 10.22 DD420 pKa = 3.85 KK421 pKa = 10.96 YY422 pKa = 10.79 VNLYY426 pKa = 7.32 TQILSVVNEE435 pKa = 3.95 VNKK438 pKa = 9.22 TVKK441 pKa = 10.24 PLAIEE446 pKa = 4.32 LNNLTYY452 pKa = 10.1 EE453 pKa = 4.23 EE454 pKa = 4.51 CVEE457 pKa = 3.42 IHH459 pKa = 6.21 RR460 pKa = 11.84 RR461 pKa = 11.84 KK462 pKa = 7.6 TEE464 pKa = 3.62 KK465 pKa = 9.78 MKK467 pKa = 11.01 QEE469 pKa = 3.92 MEE471 pKa = 4.2 DD472 pKa = 3.87 AEE474 pKa = 4.52 EE475 pKa = 4.32 EE476 pKa = 4.26 DD477 pKa = 3.85 PSKK480 pKa = 10.8 IFPGPGKK487 pKa = 10.34 IPLDD491 pKa = 3.86 EE492 pKa = 5.0 DD493 pKa = 3.3 MKK495 pKa = 11.22 KK496 pKa = 10.8 YY497 pKa = 10.57 INPHH501 pKa = 4.64 TNMSYY506 pKa = 9.87 TEE508 pKa = 4.43 EE509 pKa = 4.05 EE510 pKa = 4.56 WSNLPADD517 pKa = 4.8 IIQKK521 pKa = 9.74 IEE523 pKa = 3.82 NGEE526 pKa = 4.15 QVSVGPQTSGDD537 pKa = 3.44 GGIGVAEE544 pKa = 4.31 NQPVPPPSISEE555 pKa = 4.02 RR556 pKa = 11.84 EE557 pKa = 3.88 RR558 pKa = 11.84 EE559 pKa = 4.3 VNEE562 pKa = 4.07 EE563 pKa = 3.77 EE564 pKa = 5.14 AKK566 pKa = 10.3 QDD568 pKa = 3.65 EE569 pKa = 4.45 NNITSKK575 pKa = 10.93 SAICPHH581 pKa = 5.98 GRR583 pKa = 11.84 CRR585 pKa = 11.84 QILVEE590 pKa = 4.01 KK591 pKa = 10.11 AVRR594 pKa = 11.84 STASCRR600 pKa = 11.84 APAFVQIHH608 pKa = 4.55 HH609 pKa = 6.01 QKK611 pKa = 9.1 RR612 pKa = 11.84 TFF614 pKa = 3.1
Molecular weight: 69.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.126
IPC2_protein 9.399
IPC_protein 9.37
Toseland 10.321
ProMoST 9.853
Dawson 10.438
Bjellqvist 10.043
Wikipedia 10.555
Rodwell 11.008
Grimsley 10.482
Solomon 10.452
Lehninger 10.438
Nozaki 10.292
DTASelect 10.043
Thurlkill 10.321
EMBOSS 10.701
Sillero 10.35
Patrickios 10.687
IPC_peptide 10.467
IPC2_peptide 8.521
IPC2.peptide.svr19 8.47
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1807
165
614
301.2
34.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.317 ± 0.311
1.328 ± 0.346
4.483 ± 0.564
5.589 ± 0.527
4.815 ± 0.904
5.589 ± 0.727
2.546 ± 0.264
6.807 ± 0.808
7.305 ± 0.83
7.914 ± 1.178
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.21 ± 0.56
6.309 ± 0.708
6.53 ± 1.369
4.87 ± 0.533
5.811 ± 0.433
5.811 ± 0.503
6.032 ± 0.827
5.921 ± 0.802
1.051 ± 0.374
3.763 ± 0.659
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here