Amino acid dipepetide frequency for Pseudospirillum japonicum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.078AlaAla: 10.078 ± 0.151
1.502AlaCys: 1.502 ± 0.045
5.209AlaAsp: 5.209 ± 0.081
5.94AlaGlu: 5.94 ± 0.097
3.413AlaPhe: 3.413 ± 0.073
6.752AlaGly: 6.752 ± 0.101
3.144AlaHis: 3.144 ± 0.072
4.97AlaIle: 4.97 ± 0.077
3.741AlaLys: 3.741 ± 0.071
13.396AlaLeu: 13.396 ± 0.133
2.414AlaMet: 2.414 ± 0.054
3.201AlaAsn: 3.201 ± 0.068
4.176AlaPro: 4.176 ± 0.083
8.456AlaGln: 8.456 ± 0.161
5.81AlaArg: 5.81 ± 0.088
6.285AlaSer: 6.285 ± 0.103
4.521AlaThr: 4.521 ± 0.085
5.618AlaVal: 5.618 ± 0.101
1.766AlaTrp: 1.766 ± 0.053
3.409AlaTyr: 3.409 ± 0.073
0.0AlaXaa: 0.0 ± 0.0
Cys
1.06CysAla: 1.06 ± 0.043
0.13CysCys: 0.13 ± 0.011
0.505CysAsp: 0.505 ± 0.024
0.569CysGlu: 0.569 ± 0.027
0.355CysPhe: 0.355 ± 0.023
0.801CysGly: 0.801 ± 0.034
0.349CysHis: 0.349 ± 0.021
0.637CysIle: 0.637 ± 0.027
0.317CysLys: 0.317 ± 0.019
1.119CysLeu: 1.119 ± 0.042
0.235CysMet: 0.235 ± 0.017
0.241CysAsn: 0.241 ± 0.018
0.533CysPro: 0.533 ± 0.028
0.704CysGln: 0.704 ± 0.031
0.544CysArg: 0.544 ± 0.03
0.506CysSer: 0.506 ± 0.027
0.541CysThr: 0.541 ± 0.028
0.723CysVal: 0.723 ± 0.034
0.129CysTrp: 0.129 ± 0.013
0.295CysTyr: 0.295 ± 0.019
0.0CysXaa: 0.0 ± 0.0
Asp
5.396AspAla: 5.396 ± 0.096
0.354AspCys: 0.354 ± 0.02
2.174AspAsp: 2.174 ± 0.078
3.198AspGlu: 3.198 ± 0.071
2.312AspPhe: 2.312 ± 0.056
2.67AspGly: 2.67 ± 0.075
1.065AspHis: 1.065 ± 0.039
3.178AspIle: 3.178 ± 0.066
2.012AspLys: 2.012 ± 0.059
6.279AspLeu: 6.279 ± 0.1
1.179AspMet: 1.179 ± 0.035
1.382AspAsn: 1.382 ± 0.053
2.5AspPro: 2.5 ± 0.059
2.864AspGln: 2.864 ± 0.068
1.969AspArg: 1.969 ± 0.053
2.15AspSer: 2.15 ± 0.087
3.24AspThr: 3.24 ± 0.094
3.089AspVal: 3.089 ± 0.066
0.795AspTrp: 0.795 ± 0.032
1.833AspTyr: 1.833 ± 0.05
0.0AspXaa: 0.0 ± 0.0
Glu
6.498GluAla: 6.498 ± 0.105
0.432GluCys: 0.432 ± 0.026
2.859GluAsp: 2.859 ± 0.069
3.536GluGlu: 3.536 ± 0.081
2.067GluPhe: 2.067 ± 0.056
3.567GluGly: 3.567 ± 0.076
1.699GluHis: 1.699 ± 0.044
3.764GluIle: 3.764 ± 0.065
2.612GluLys: 2.612 ± 0.079
6.365GluLeu: 6.365 ± 0.103
1.307GluMet: 1.307 ± 0.043
1.919GluAsn: 1.919 ± 0.052
1.778GluPro: 1.778 ± 0.056
4.202GluGln: 4.202 ± 0.096
3.584GluArg: 3.584 ± 0.073
2.7GluSer: 2.7 ± 0.063
3.352GluThr: 3.352 ± 0.104
4.458GluVal: 4.458 ± 0.075
0.574GluTrp: 0.574 ± 0.028
1.819GluTyr: 1.819 ± 0.048
0.0GluXaa: 0.0 ± 0.0
Phe
3.812PheAla: 3.812 ± 0.073
0.483PheCys: 0.483 ± 0.026
2.095PheAsp: 2.095 ± 0.047
1.782PheGlu: 1.782 ± 0.048
1.377PhePhe: 1.377 ± 0.048
2.379PheGly: 2.379 ± 0.059
0.805PheHis: 0.805 ± 0.03
2.178PheIle: 2.178 ± 0.061
1.389PheLys: 1.389 ± 0.043
3.3PheLeu: 3.3 ± 0.079
0.869PheMet: 0.869 ± 0.034
1.491PheAsn: 1.491 ± 0.039
1.266PhePro: 1.266 ± 0.04
1.505PheGln: 1.505 ± 0.046
1.558PheArg: 1.558 ± 0.042
2.424PheSer: 2.424 ± 0.051
2.049PheThr: 2.049 ± 0.052
2.314PheVal: 2.314 ± 0.061
0.574PheTrp: 0.574 ± 0.026
1.257PheTyr: 1.257 ± 0.044
0.0PheXaa: 0.0 ± 0.0
Gly
5.559GlyAla: 5.559 ± 0.119
0.824GlyCys: 0.824 ± 0.034
3.127GlyAsp: 3.127 ± 0.078
3.947GlyGlu: 3.947 ± 0.063
2.848GlyPhe: 2.848 ± 0.064
4.365GlyGly: 4.365 ± 0.133
1.94GlyHis: 1.94 ± 0.048
4.357GlyIle: 4.357 ± 0.08
2.635GlyLys: 2.635 ± 0.062
7.422GlyLeu: 7.422 ± 0.104
1.836GlyMet: 1.836 ± 0.052
1.703GlyAsn: 1.703 ± 0.066
1.646GlyPro: 1.646 ± 0.046
3.747GlyGln: 3.747 ± 0.07
3.825GlyArg: 3.825 ± 0.067
3.192GlySer: 3.192 ± 0.078
3.2GlyThr: 3.2 ± 0.086
4.845GlyVal: 4.845 ± 0.086
1.005GlyTrp: 1.005 ± 0.04
2.35GlyTyr: 2.35 ± 0.05
0.0GlyXaa: 0.0 ± 0.0
His
2.581HisAla: 2.581 ± 0.062
0.289HisCys: 0.289 ± 0.02
0.879HisAsp: 0.879 ± 0.033
1.262HisGlu: 1.262 ± 0.041
1.267HisPhe: 1.267 ± 0.042
1.343HisGly: 1.343 ± 0.036
0.819HisHis: 0.819 ± 0.033
1.528HisIle: 1.528 ± 0.04
1.082HisLys: 1.082 ± 0.041
4.234HisLeu: 4.234 ± 0.097
0.606HisMet: 0.606 ± 0.028
0.708HisAsn: 0.708 ± 0.029
1.897HisPro: 1.897 ± 0.055
2.284HisGln: 2.284 ± 0.058
1.132HisArg: 1.132 ± 0.037
1.291HisSer: 1.291 ± 0.042
1.79HisThr: 1.79 ± 0.061
1.25HisVal: 1.25 ± 0.039
0.608HisTrp: 0.608 ± 0.026
0.922HisTyr: 0.922 ± 0.032
0.0HisXaa: 0.0 ± 0.0
Ile
5.391IleAla: 5.391 ± 0.091
0.629IleCys: 0.629 ± 0.03
3.108IleAsp: 3.108 ± 0.069
3.778IleGlu: 3.778 ± 0.065
1.95IlePhe: 1.95 ± 0.056
3.769IleGly: 3.769 ± 0.076
1.429IleHis: 1.429 ± 0.041
2.525IleIle: 2.525 ± 0.069
2.319IleLys: 2.319 ± 0.061
5.79IleLeu: 5.79 ± 0.086
0.983IleMet: 0.983 ± 0.036
2.251IleAsn: 2.251 ± 0.056
2.438IlePro: 2.438 ± 0.063
3.624IleGln: 3.624 ± 0.075
3.256IleArg: 3.256 ± 0.067
3.795IleSer: 3.795 ± 0.08
2.862IleThr: 2.862 ± 0.065
2.858IleVal: 2.858 ± 0.059
0.604IleTrp: 0.604 ± 0.028
1.675IleTyr: 1.675 ± 0.046
0.0IleXaa: 0.0 ± 0.0
Lys
4.136LysAla: 4.136 ± 0.084
0.257LysCys: 0.257 ± 0.019
1.951LysAsp: 1.951 ± 0.054
2.239LysGlu: 2.239 ± 0.059
1.001LysPhe: 1.001 ± 0.034
2.319LysGly: 2.319 ± 0.06
1.011LysHis: 1.011 ± 0.034
2.529LysIle: 2.529 ± 0.056
1.823LysLys: 1.823 ± 0.066
3.983LysLeu: 3.983 ± 0.069
0.982LysMet: 0.982 ± 0.033
1.435LysAsn: 1.435 ± 0.048
1.688LysPro: 1.688 ± 0.046
2.296LysGln: 2.296 ± 0.057
2.408LysArg: 2.408 ± 0.061
2.186LysSer: 2.186 ± 0.058
2.238LysThr: 2.238 ± 0.056
2.951LysVal: 2.951 ± 0.066
0.299LysTrp: 0.299 ± 0.02
1.075LysTyr: 1.075 ± 0.033
0.0LysXaa: 0.0 ± 0.0
Leu
13.788LeuAla: 13.788 ± 0.152
1.101LeuCys: 1.101 ± 0.041
6.553LeuAsp: 6.553 ± 0.099
7.03LeuGlu: 7.03 ± 0.116
3.608LeuPhe: 3.608 ± 0.084
8.253LeuGly: 8.253 ± 0.114
3.023LeuHis: 3.023 ± 0.075
6.287LeuIle: 6.287 ± 0.103
4.671LeuLys: 4.671 ± 0.095
12.556LeuLeu: 12.556 ± 0.168
2.708LeuMet: 2.708 ± 0.066
3.958LeuAsn: 3.958 ± 0.071
5.731LeuPro: 5.731 ± 0.105
7.407LeuGln: 7.407 ± 0.136
5.438LeuArg: 5.438 ± 0.099
6.979LeuSer: 6.979 ± 0.096
6.337LeuThr: 6.337 ± 0.097
7.775LeuVal: 7.775 ± 0.098
1.512LeuTrp: 1.512 ± 0.042
3.055LeuTyr: 3.055 ± 0.064
0.0LeuXaa: 0.0 ± 0.0
Met
2.368MetAla: 2.368 ± 0.058
0.187MetCys: 0.187 ± 0.014
1.063MetAsp: 1.063 ± 0.039
0.966MetGlu: 0.966 ± 0.033
0.618MetPhe: 0.618 ± 0.027
1.51MetGly: 1.51 ± 0.051
0.663MetHis: 0.663 ± 0.03
1.093MetIle: 1.093 ± 0.037
0.828MetLys: 0.828 ± 0.031
2.628MetLeu: 2.628 ± 0.069
0.551MetMet: 0.551 ± 0.03
0.7MetAsn: 0.7 ± 0.032
1.345MetPro: 1.345 ± 0.039
2.031MetGln: 2.031 ± 0.053
1.513MetArg: 1.513 ± 0.044
1.649MetSer: 1.649 ± 0.045
1.199MetThr: 1.199 ± 0.036
1.178MetVal: 1.178 ± 0.04
0.18MetTrp: 0.18 ± 0.015
0.458MetTyr: 0.458 ± 0.024
0.0MetXaa: 0.0 ± 0.0
Asn
3.213AsnAla: 3.213 ± 0.073
0.267AsnCys: 0.267 ± 0.018
1.175AsnAsp: 1.175 ± 0.043
1.599AsnGlu: 1.599 ± 0.051
1.156AsnPhe: 1.156 ± 0.044
1.663AsnGly: 1.663 ± 0.062
0.721AsnHis: 0.721 ± 0.03
1.928AsnIle: 1.928 ± 0.059
1.249AsnLys: 1.249 ± 0.046
3.947AsnLeu: 3.947 ± 0.076
0.688AsnMet: 0.688 ± 0.029
0.971AsnAsn: 0.971 ± 0.044
2.014AsnPro: 2.014 ± 0.054
2.333AsnGln: 2.333 ± 0.054
1.561AsnArg: 1.561 ± 0.039
1.424AsnSer: 1.424 ± 0.054
2.101AsnThr: 2.101 ± 0.055
1.56AsnVal: 1.56 ± 0.045
0.387AsnTrp: 0.387 ± 0.021
0.906AsnTyr: 0.906 ± 0.033
0.0AsnXaa: 0.0 ± 0.0
Pro
4.051ProAla: 4.051 ± 0.082
0.417ProCys: 0.417 ± 0.025
2.27ProAsp: 2.27 ± 0.06
3.901ProGlu: 3.901 ± 0.069
1.495ProPhe: 1.495 ± 0.044
1.963ProGly: 1.963 ± 0.055
1.257ProHis: 1.257 ± 0.042
2.296ProIle: 2.296 ± 0.05
2.041ProLys: 2.041 ± 0.049
5.236ProLeu: 5.236 ± 0.092
1.012ProMet: 1.012 ± 0.035
1.366ProAsn: 1.366 ± 0.043
1.718ProPro: 1.718 ± 0.055
3.313ProGln: 3.313 ± 0.077
1.69ProArg: 1.69 ± 0.05
2.635ProSer: 2.635 ± 0.056
2.152ProThr: 2.152 ± 0.07
3.201ProVal: 3.201 ± 0.065
0.85ProTrp: 0.85 ± 0.034
1.364ProTyr: 1.364 ± 0.041
0.0ProXaa: 0.0 ± 0.0
Gln
11.078GlnAla: 11.078 ± 0.21
0.417GlnCys: 0.417 ± 0.023
3.626GlnAsp: 3.626 ± 0.078
4.465GlnGlu: 4.465 ± 0.074
1.277GlnPhe: 1.277 ± 0.039
5.529GlnGly: 5.529 ± 0.095
2.119GlnHis: 2.119 ± 0.06
3.525GlnIle: 3.525 ± 0.076
2.579GlnLys: 2.579 ± 0.06
6.488GlnLeu: 6.488 ± 0.128
1.436GlnMet: 1.436 ± 0.046
1.752GlnAsn: 1.752 ± 0.046
2.408GlnPro: 2.408 ± 0.064
5.685GlnGln: 5.685 ± 0.139
3.497GlnArg: 3.497 ± 0.066
2.977GlnSer: 2.977 ± 0.068
3.704GlnThr: 3.704 ± 0.081
5.823GlnVal: 5.823 ± 0.097
0.852GlnTrp: 0.852 ± 0.03
1.468GlnTyr: 1.468 ± 0.047
0.0GlnXaa: 0.0 ± 0.0
Arg
4.695ArgAla: 4.695 ± 0.082
0.483ArgCys: 0.483 ± 0.026
2.566ArgAsp: 2.566 ± 0.059
3.19ArgGlu: 3.19 ± 0.072
2.146ArgPhe: 2.146 ± 0.052
2.809ArgGly: 2.809 ± 0.054
1.652ArgHis: 1.652 ± 0.05
3.212ArgIle: 3.212 ± 0.064
2.028ArgLys: 2.028 ± 0.064
7.068ArgLeu: 7.068 ± 0.102
1.308ArgMet: 1.308 ± 0.041
1.436ArgAsn: 1.436 ± 0.041
2.217ArgPro: 2.217 ± 0.047
3.8ArgGln: 3.8 ± 0.072
3.061ArgArg: 3.061 ± 0.071
2.316ArgSer: 2.316 ± 0.065
2.873ArgThr: 2.873 ± 0.058
3.4ArgVal: 3.4 ± 0.07
0.868ArgTrp: 0.868 ± 0.035
1.762ArgTyr: 1.762 ± 0.048
0.0ArgXaa: 0.0 ± 0.0
Ser
5.626SerAla: 5.626 ± 0.084
0.561SerCys: 0.561 ± 0.027
2.355SerAsp: 2.355 ± 0.065
3.05SerGlu: 3.05 ± 0.084
2.228SerPhe: 2.228 ± 0.052
3.995SerGly: 3.995 ± 0.081
1.321SerHis: 1.321 ± 0.035
3.113SerIle: 3.113 ± 0.069
1.961SerLys: 1.961 ± 0.052
7.289SerLeu: 7.289 ± 0.101
1.38SerMet: 1.38 ± 0.043
1.463SerAsn: 1.463 ± 0.046
2.251SerPro: 2.251 ± 0.047
3.523SerGln: 3.523 ± 0.078
2.764SerArg: 2.764 ± 0.062
3.216SerSer: 3.216 ± 0.073
3.097SerThr: 3.097 ± 0.082
3.756SerVal: 3.756 ± 0.075
0.776SerTrp: 0.776 ± 0.033
1.533SerTyr: 1.533 ± 0.041
0.0SerXaa: 0.0 ± 0.0
Thr
4.229ThrAla: 4.229 ± 0.088
0.732ThrCys: 0.732 ± 0.031
2.741ThrAsp: 2.741 ± 0.095
2.983ThrGlu: 2.983 ± 0.076
1.733ThrPhe: 1.733 ± 0.045
4.003ThrGly: 4.003 ± 0.081
1.74ThrHis: 1.74 ± 0.049
1.675ThrIle: 1.675 ± 0.063
1.583ThrLys: 1.583 ± 0.046
7.37ThrLeu: 7.37 ± 0.102
0.761ThrMet: 0.761 ± 0.028
1.496ThrAsn: 1.496 ± 0.056
3.531ThrPro: 3.531 ± 0.076
4.617ThrGln: 4.617 ± 0.085
2.906ThrArg: 2.906 ± 0.062
3.64ThrSer: 3.64 ± 0.085
2.428ThrThr: 2.428 ± 0.082
2.311ThrVal: 2.311 ± 0.082
1.027ThrTrp: 1.027 ± 0.043
1.827ThrTyr: 1.827 ± 0.051
0.0ThrXaa: 0.0 ± 0.0
Val
6.223ValAla: 6.223 ± 0.089
0.723ValCys: 0.723 ± 0.03
3.551ValAsp: 3.551 ± 0.08
3.819ValGlu: 3.819 ± 0.075
2.281ValPhe: 2.281 ± 0.055
3.959ValGly: 3.959 ± 0.087
1.744ValHis: 1.744 ± 0.046
3.937ValIle: 3.937 ± 0.068
2.609ValLys: 2.609 ± 0.067
7.291ValLeu: 7.291 ± 0.1
1.569ValMet: 1.569 ± 0.049
2.302ValAsn: 2.302 ± 0.058
2.619ValPro: 2.619 ± 0.061
3.695ValGln: 3.695 ± 0.08
3.613ValArg: 3.613 ± 0.059
3.73ValSer: 3.73 ± 0.069
3.503ValThr: 3.503 ± 0.084
4.203ValVal: 4.203 ± 0.084
0.783ValTrp: 0.783 ± 0.031
1.812ValTyr: 1.812 ± 0.044
0.0ValXaa: 0.0 ± 0.0
Trp
1.033TrpAla: 1.033 ± 0.035
0.205TrpCys: 0.205 ± 0.014
0.588TrpAsp: 0.588 ± 0.027
0.534TrpGlu: 0.534 ± 0.023
0.541TrpPhe: 0.541 ± 0.026
0.816TrpGly: 0.816 ± 0.034
0.483TrpHis: 0.483 ± 0.025
0.866TrpIle: 0.866 ± 0.034
0.376TrpLys: 0.376 ± 0.022
2.044TrpLeu: 2.044 ± 0.065
0.382TrpMet: 0.382 ± 0.021
0.326TrpAsn: 0.326 ± 0.022
0.785TrpPro: 0.785 ± 0.036
1.636TrpGln: 1.636 ± 0.06
0.977TrpArg: 0.977 ± 0.033
0.674TrpSer: 0.674 ± 0.03
0.479TrpThr: 0.479 ± 0.025
1.01TrpVal: 1.01 ± 0.045
0.29TrpTrp: 0.29 ± 0.021
0.383TrpTyr: 0.383 ± 0.024
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.989TyrAla: 2.989 ± 0.065
0.301TyrCys: 0.301 ± 0.019
1.241TyrAsp: 1.241 ± 0.045
1.306TyrGlu: 1.306 ± 0.046
1.233TyrPhe: 1.233 ± 0.048
1.91TyrGly: 1.91 ± 0.049
0.79TyrHis: 0.79 ± 0.033
1.435TyrIle: 1.435 ± 0.048
0.952TyrLys: 0.952 ± 0.034
3.965TyrLeu: 3.965 ± 0.077
0.518TyrMet: 0.518 ± 0.024
0.758TyrAsn: 0.758 ± 0.032
1.688TyrPro: 1.688 ± 0.049
2.986TyrGln: 2.986 ± 0.073
1.782TyrArg: 1.782 ± 0.044
1.489TyrSer: 1.489 ± 0.043
1.664TyrThr: 1.664 ± 0.044
1.649TyrVal: 1.649 ± 0.05
0.548TyrTrp: 0.548 ± 0.027
0.91TyrTyr: 0.91 ± 0.038
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2385 proteins (820087 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski