Lynx canadensis faeces associated genomovirus CL4 128
Average proteome isoelectric point is 7.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z5CL64|A0A2Z5CL64_9VIRU Capsid protein OS=Lynx canadensis faeces associated genomovirus CL4 128 OX=2219130 PE=4 SV=1
MM1 pKa = 7.89 PSFHH5 pKa = 6.86 LKK7 pKa = 8.75 NRR9 pKa = 11.84 RR10 pKa = 11.84 YY11 pKa = 10.69 VLFTYY16 pKa = 10.13 AQAGTDD22 pKa = 3.68 FDD24 pKa = 3.84 YY25 pKa = 10.81 WAVVDD30 pKa = 4.77 LLGGLGAEE38 pKa = 4.56 CIIGRR43 pKa = 11.84 EE44 pKa = 4.04 SHH46 pKa = 6.91 ADD48 pKa = 3.34 GGIHH52 pKa = 5.69 FHH54 pKa = 6.29 VFADD58 pKa = 4.61 FGRR61 pKa = 11.84 LFSRR65 pKa = 11.84 KK66 pKa = 7.46 TDD68 pKa = 3.54 LFDD71 pKa = 4.53 VGGKK75 pKa = 9.29 HH76 pKa = 6.83 PNIQPIGRR84 pKa = 11.84 TPAKK88 pKa = 10.3 AYY90 pKa = 10.39 DD91 pKa = 3.93 YY92 pKa = 10.86 ACKK95 pKa = 10.72 DD96 pKa = 3.23 GDD98 pKa = 4.09 VVAGGLGRR106 pKa = 11.84 PGGDD110 pKa = 3.43 ADD112 pKa = 4.94 FDD114 pKa = 4.67 PDD116 pKa = 4.67 SFWDD120 pKa = 3.7 SATHH124 pKa = 6.59 CGSADD129 pKa = 3.65 EE130 pKa = 4.98 FLHH133 pKa = 7.02 FCDD136 pKa = 4.14 QLAPRR141 pKa = 11.84 DD142 pKa = 4.09 LIRR145 pKa = 11.84 GFPAFRR151 pKa = 11.84 SYY153 pKa = 11.85 SNWKK157 pKa = 8.65 WNDD160 pKa = 2.56 GSLVYY165 pKa = 10.17 NQPDD169 pKa = 3.4 GVEE172 pKa = 4.29 FDD174 pKa = 3.38 TSAAEE179 pKa = 4.92 GIDD182 pKa = 3.48 EE183 pKa = 4.26 RR184 pKa = 11.84 RR185 pKa = 11.84 SLVLFGPYY193 pKa = 10.43 GCGKK197 pKa = 5.65 TTWARR202 pKa = 11.84 SLGEE206 pKa = 4.02 HH207 pKa = 6.89 IYY209 pKa = 10.68 FGSQWSGKK217 pKa = 7.76 VAFQGLEE224 pKa = 3.77 KK225 pKa = 10.81 AEE227 pKa = 3.85 YY228 pKa = 10.37 AIFDD232 pKa = 3.71 DD233 pKa = 3.95 WKK235 pKa = 10.84 GGLKK239 pKa = 9.14 MLPGYY244 pKa = 10.21 KK245 pKa = 9.65 DD246 pKa = 2.9 WLGAQWHH253 pKa = 5.04 VSVRR257 pKa = 11.84 QLHH260 pKa = 6.14 HH261 pKa = 7.14 DD262 pKa = 3.37 ARR264 pKa = 11.84 LEE266 pKa = 3.93 EE267 pKa = 4.12 WGRR270 pKa = 11.84 PCIWLCNTDD279 pKa = 3.88 PRR281 pKa = 11.84 VMTHH285 pKa = 6.19 VNDD288 pKa = 3.84 DD289 pKa = 4.22 TDD291 pKa = 3.91 WTWMDD296 pKa = 3.94 RR297 pKa = 11.84 ACIFVEE303 pKa = 4.35 VTGALATFRR312 pKa = 11.84 ANTVV316 pKa = 2.82
Molecular weight: 35.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.209
IPC2_protein 5.181
IPC_protein 5.181
Toseland 5.372
ProMoST 5.461
Dawson 5.334
Bjellqvist 5.397
Wikipedia 5.308
Rodwell 5.258
Grimsley 5.423
Solomon 5.334
Lehninger 5.308
Nozaki 5.525
DTASelect 5.753
Thurlkill 5.525
EMBOSS 5.525
Sillero 5.601
Patrickios 3.821
IPC_peptide 5.347
IPC2_peptide 5.601
IPC2.peptide.svr19 5.577
Protein with the highest isoelectric point:
>tr|A0A2Z5CL64|A0A2Z5CL64_9VIRU Capsid protein OS=Lynx canadensis faeces associated genomovirus CL4 128 OX=2219130 PE=4 SV=1
MM1 pKa = 7.79 AYY3 pKa = 10.19 ARR5 pKa = 11.84 RR6 pKa = 11.84 LRR8 pKa = 11.84 RR9 pKa = 11.84 PAYY12 pKa = 8.89 RR13 pKa = 11.84 KK14 pKa = 9.29 KK15 pKa = 10.21 AARR18 pKa = 11.84 STRR21 pKa = 11.84 RR22 pKa = 11.84 YY23 pKa = 9.73 SKK25 pKa = 10.25 RR26 pKa = 11.84 SYY28 pKa = 10.02 PRR30 pKa = 11.84 SSYY33 pKa = 9.91 VKK35 pKa = 9.42 KK36 pKa = 10.07 RR37 pKa = 11.84 PYY39 pKa = 9.53 RR40 pKa = 11.84 KK41 pKa = 9.95 RR42 pKa = 11.84 MTKK45 pKa = 10.34 KK46 pKa = 10.35 SILNVTSRR54 pKa = 11.84 KK55 pKa = 9.78 KK56 pKa = 10.11 RR57 pKa = 11.84 DD58 pKa = 2.95 TMAYY62 pKa = 6.99 YY63 pKa = 9.89 TNSSATSQTGSTTYY77 pKa = 10.47 VQDD80 pKa = 3.47 AAVVTGGSSVPACFLWCATGRR101 pKa = 11.84 DD102 pKa = 4.19 YY103 pKa = 8.2 TTSNAGTNGQIEE115 pKa = 4.5 DD116 pKa = 3.92 SATRR120 pKa = 11.84 TAQSCYY126 pKa = 8.33 MRR128 pKa = 11.84 GLKK131 pKa = 9.53 EE132 pKa = 3.77 ALEE135 pKa = 4.24 VQVADD140 pKa = 3.84 GLPWQWRR147 pKa = 11.84 RR148 pKa = 11.84 ICFTAKK154 pKa = 10.43 GLQTLFASTTGFSLVIEE171 pKa = 4.78 ASTGFQRR178 pKa = 11.84 VLNQVPGDD186 pKa = 3.81 SGSPNSLRR194 pKa = 11.84 AIIEE198 pKa = 4.1 TLLFRR203 pKa = 11.84 GVYY206 pKa = 9.56 LRR208 pKa = 11.84 DD209 pKa = 3.61 WIDD212 pKa = 3.32 PMTAKK217 pKa = 9.84 TDD219 pKa = 3.56 NDD221 pKa = 3.91 RR222 pKa = 11.84 LTIKK226 pKa = 10.27 YY227 pKa = 9.88 DD228 pKa = 3.3 KK229 pKa = 9.82 TISIASGNEE238 pKa = 3.73 DD239 pKa = 3.32 GCIRR243 pKa = 11.84 SYY245 pKa = 11.15 KK246 pKa = 9.46 RR247 pKa = 11.84 WHH249 pKa = 6.44 GMNKK253 pKa = 9.84 SLVYY257 pKa = 10.78 NDD259 pKa = 4.86 DD260 pKa = 3.47 EE261 pKa = 5.39 SGGTKK266 pKa = 10.0 VPAFYY271 pKa = 9.7 STLGKK276 pKa = 10.56 AGMGDD281 pKa = 4.59 FYY283 pKa = 11.76 VMDD286 pKa = 4.04 YY287 pKa = 10.14 FRR289 pKa = 11.84 PRR291 pKa = 11.84 AGSTSSNQLRR301 pKa = 11.84 VAASSTLYY309 pKa = 9.44 WHH311 pKa = 7.06 EE312 pKa = 4.1 KK313 pKa = 9.32
Molecular weight: 35.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.283
IPC2_protein 9.604
IPC_protein 9.94
Toseland 10.058
ProMoST 9.867
Dawson 10.306
Bjellqvist 10.028
Wikipedia 10.511
Rodwell 10.584
Grimsley 10.394
Solomon 10.335
Lehninger 10.277
Nozaki 10.072
DTASelect 10.014
Thurlkill 10.145
EMBOSS 10.482
Sillero 10.218
Patrickios 9.809
IPC_peptide 10.321
IPC2_peptide 8.916
IPC2.peptide.svr19 8.554
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
629
313
316
314.5
35.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.585 ± 0.031
2.067 ± 0.352
7.472 ± 1.77
3.18 ± 0.468
4.769 ± 1.42
9.221 ± 1.404
2.226 ± 1.19
3.498 ± 0.013
5.087 ± 0.977
6.677 ± 0.455
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.908 ± 0.486
3.021 ± 0.37
3.657 ± 0.346
2.862 ± 0.25
7.79 ± 1.346
7.631 ± 2.184
7.154 ± 1.823
5.405 ± 0.22
3.021 ± 0.828
4.769 ± 0.975
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here