Alces alces faeces associated genomovirus MP157
Average proteome isoelectric point is 7.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z5CJA5|A0A2Z5CJA5_9VIRU Replication associated protein OS=Alces alces faeces associated genomovirus MP157 OX=2219113 PE=4 SV=1
MM1 pKa = 7.72 PFDD4 pKa = 3.32 FHH6 pKa = 8.54 SRR8 pKa = 11.84 YY9 pKa = 10.7 GLFTYY14 pKa = 10.71 AHH16 pKa = 6.52 VDD18 pKa = 3.44 EE19 pKa = 5.93 LDD21 pKa = 3.6 PFCIVEE27 pKa = 4.48 HH28 pKa = 6.37 FSSLGGEE35 pKa = 4.44 CIVAAEE41 pKa = 5.01 RR42 pKa = 11.84 YY43 pKa = 6.0 PTGPGVHH50 pKa = 5.25 YY51 pKa = 10.57 HH52 pKa = 5.67 VFTDD56 pKa = 3.96 FGRR59 pKa = 11.84 KK60 pKa = 7.93 FRR62 pKa = 11.84 SRR64 pKa = 11.84 RR65 pKa = 11.84 VDD67 pKa = 3.45 CFDD70 pKa = 3.29 VAGFHH75 pKa = 6.56 PNIVASRR82 pKa = 11.84 GTPEE86 pKa = 4.14 AGYY89 pKa = 10.62 DD90 pKa = 3.63 YY91 pKa = 10.68 AIKK94 pKa = 10.92 DD95 pKa = 3.59 GDD97 pKa = 4.01 VVAGGLEE104 pKa = 4.06 RR105 pKa = 11.84 PSGVEE110 pKa = 3.23 PDD112 pKa = 3.3 TRR114 pKa = 11.84 EE115 pKa = 4.4 TKK117 pKa = 7.53 WHH119 pKa = 7.25 RR120 pKa = 11.84 ITSAATRR127 pKa = 11.84 EE128 pKa = 4.32 EE129 pKa = 4.38 FFHH132 pKa = 7.27 LLRR135 pKa = 11.84 TLDD138 pKa = 3.82 PKK140 pKa = 11.34 SLVTNHH146 pKa = 6.88 RR147 pKa = 11.84 SCIAFADD154 pKa = 3.34 WQYY157 pKa = 11.12 RR158 pKa = 11.84 VEE160 pKa = 4.15 PTPYY164 pKa = 8.89 ATPNGVFNLAEE175 pKa = 4.28 YY176 pKa = 11.1 GDD178 pKa = 4.3 LSEE181 pKa = 4.87 SRR183 pKa = 11.84 SLVLFGDD190 pKa = 3.55 TRR192 pKa = 11.84 LGKK195 pKa = 7.65 TVWSRR200 pKa = 11.84 SLGPHH205 pKa = 6.34 VYY207 pKa = 10.15 FCGLYY212 pKa = 10.18 SYY214 pKa = 11.49 AEE216 pKa = 4.04 AVKK219 pKa = 10.8 APDD222 pKa = 2.58 VDD224 pKa = 3.94 YY225 pKa = 11.48 AVFDD229 pKa = 5.38 DD230 pKa = 3.82 IQGGIKK236 pKa = 10.07 FFPAFKK242 pKa = 10.48 NWLGCQMEE250 pKa = 4.38 FQVKK254 pKa = 9.64 GLYY257 pKa = 9.72 RR258 pKa = 11.84 DD259 pKa = 3.68 PQLLKK264 pKa = 9.92 WGKK267 pKa = 9.04 PSIWVANTDD276 pKa = 3.34 PRR278 pKa = 11.84 HH279 pKa = 6.79 DD280 pKa = 3.88 MTHH283 pKa = 7.45 DD284 pKa = 4.47 DD285 pKa = 4.22 IKK287 pKa = 10.95 WMEE290 pKa = 4.11 GNCIFVEE297 pKa = 4.19 IVSAIFHH304 pKa = 6.95 ASTGYY309 pKa = 8.9 PACGEE314 pKa = 4.28 TAEE317 pKa = 4.59 SPP319 pKa = 3.6
Molecular weight: 36.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.349
IPC2_protein 5.347
IPC_protein 5.372
Toseland 5.69
ProMoST 5.664
Dawson 5.563
Bjellqvist 5.601
Wikipedia 5.537
Rodwell 5.525
Grimsley 5.804
Solomon 5.563
Lehninger 5.55
Nozaki 5.792
DTASelect 5.982
Thurlkill 5.919
EMBOSS 5.881
Sillero 5.881
Patrickios 3.617
IPC_peptide 5.575
IPC2_peptide 5.906
IPC2.peptide.svr19 5.817
Protein with the highest isoelectric point:
>tr|A0A2Z5CJA5|A0A2Z5CJA5_9VIRU Replication associated protein OS=Alces alces faeces associated genomovirus MP157 OX=2219113 PE=4 SV=1
MM1 pKa = 7.74 GYY3 pKa = 10.68 AKK5 pKa = 10.22 RR6 pKa = 11.84 RR7 pKa = 11.84 STSSRR12 pKa = 11.84 RR13 pKa = 11.84 TTRR16 pKa = 11.84 GPYY19 pKa = 9.47 RR20 pKa = 11.84 RR21 pKa = 11.84 YY22 pKa = 6.46 TAKK25 pKa = 10.25 RR26 pKa = 11.84 RR27 pKa = 11.84 SLGKK31 pKa = 9.93 AKK33 pKa = 10.13 RR34 pKa = 11.84 PYY36 pKa = 9.85 RR37 pKa = 11.84 RR38 pKa = 11.84 TYY40 pKa = 9.9 RR41 pKa = 11.84 KK42 pKa = 10.15 KK43 pKa = 10.04 PMTKK47 pKa = 9.69 RR48 pKa = 11.84 RR49 pKa = 11.84 ILNATSTKK57 pKa = 10.49 KK58 pKa = 10.11 KK59 pKa = 10.87 DD60 pKa = 3.43 NMLISTNTTAANPIGGATYY79 pKa = 7.59 TTNPAILIGAQTFDD93 pKa = 3.45 YY94 pKa = 10.66 VFPFLCTYY102 pKa = 10.94 RR103 pKa = 11.84 DD104 pKa = 3.53 FTTRR108 pKa = 11.84 TGGSHH113 pKa = 6.54 GFASDD118 pKa = 2.92 TSTRR122 pKa = 11.84 SASTVFMRR130 pKa = 11.84 GYY132 pKa = 9.82 KK133 pKa = 9.84 EE134 pKa = 5.04 CITIQTSDD142 pKa = 3.48 GVPWLWRR149 pKa = 11.84 RR150 pKa = 11.84 ILISLKK156 pKa = 10.31 GPLIAEE162 pKa = 4.26 ISSVFNVAQEE172 pKa = 3.95 NSAGFTRR179 pKa = 11.84 VVNAANPGQRR189 pKa = 11.84 ANVYY193 pKa = 10.57 NLIFDD198 pKa = 4.65 GTQDD202 pKa = 4.1 ADD204 pKa = 3.54 WNSPIDD210 pKa = 3.71 AKK212 pKa = 10.88 LDD214 pKa = 3.85 RR215 pKa = 11.84 NRR217 pKa = 11.84 VTVMYY222 pKa = 10.26 DD223 pKa = 3.01 KK224 pKa = 10.93 TITLASGNEE233 pKa = 3.85 DD234 pKa = 3.48 GMIRR238 pKa = 11.84 KK239 pKa = 8.88 YY240 pKa = 10.94 NRR242 pKa = 11.84 WHH244 pKa = 6.45 GFNKK248 pKa = 10.02 NFVYY252 pKa = 10.74 DD253 pKa = 3.76 DD254 pKa = 4.34 DD255 pKa = 4.43 KK256 pKa = 11.98 AGGQEE261 pKa = 4.03 NGSGYY266 pKa = 9.98 HH267 pKa = 5.62 AQGKK271 pKa = 10.22 AGMGDD276 pKa = 3.65 VYY278 pKa = 11.09 VIDD281 pKa = 3.61 IFRR284 pKa = 11.84 PRR286 pKa = 11.84 TGTTTATQLSLHH298 pKa = 5.61 MQGTLYY304 pKa = 9.8 WHH306 pKa = 6.96 EE307 pKa = 4.2 KK308 pKa = 9.34
Molecular weight: 34.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.29
IPC2_protein 9.75
IPC_protein 10.277
Toseland 10.321
ProMoST 10.072
Dawson 10.511
Bjellqvist 10.204
Wikipedia 10.701
Rodwell 10.833
Grimsley 10.584
Solomon 10.54
Lehninger 10.511
Nozaki 10.292
DTASelect 10.204
Thurlkill 10.365
EMBOSS 10.716
Sillero 10.423
Patrickios 10.423
IPC_peptide 10.54
IPC2_peptide 9.019
IPC2.peptide.svr19 8.679
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
627
308
319
313.5
35.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.656 ± 0.091
1.595 ± 0.63
6.38 ± 0.573
3.987 ± 1.36
5.582 ± 1.124
8.612 ± 0.103
2.871 ± 0.832
4.785 ± 0.49
4.944 ± 0.817
5.423 ± 0.368
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.914 ± 0.456
3.987 ± 1.238
4.944 ± 0.915
2.233 ± 0.46
7.656 ± 1.174
6.22 ± 0.182
8.453 ± 1.941
5.901 ± 1.12
1.914 ± 0.194
4.944 ± 0.167
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here