Thalassotalea mangrovi
Average proteome isoelectric point is 6.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3157 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4U1B4Z6|A0A4U1B4Z6_9GAMM Uncharacterized protein OS=Thalassotalea mangrovi OX=2572245 GN=E8M12_09540 PE=4 SV=1
MM1 pKa = 7.9 EE2 pKa = 5.15 YY3 pKa = 8.91 NTSEE7 pKa = 4.19 LCNLYY12 pKa = 9.93 TDD14 pKa = 4.41 LVDD17 pKa = 3.64 VLEE20 pKa = 4.91 PMLSNYY26 pKa = 9.79 GGRR29 pKa = 11.84 SSFGGQVVTIKK40 pKa = 10.68 CFEE43 pKa = 4.5 ANGLISQMVEE53 pKa = 3.22 NDD55 pKa = 3.28 GTGKK59 pKa = 10.37 VLVIDD64 pKa = 4.47 GGGSARR70 pKa = 11.84 KK71 pKa = 9.52 ALVDD75 pKa = 3.61 SYY77 pKa = 11.0 IAEE80 pKa = 4.18 AAAKK84 pKa = 9.8 NGWEE88 pKa = 4.48 GIICYY93 pKa = 10.4 GSVRR97 pKa = 11.84 DD98 pKa = 3.69 VDD100 pKa = 3.85 ALEE103 pKa = 5.06 EE104 pKa = 4.08 IDD106 pKa = 4.71 IGIQAMVSIPVGAVDD121 pKa = 4.76 NDD123 pKa = 3.76 DD124 pKa = 4.53 GEE126 pKa = 4.96 IDD128 pKa = 3.77 VAVNFAGVTILPEE141 pKa = 3.69 DD142 pKa = 4.7 HH143 pKa = 7.24 IYY145 pKa = 11.29 ADD147 pKa = 3.46 NTGVVLSPDD156 pKa = 3.74 PLDD159 pKa = 4.11 IEE161 pKa = 4.46
Molecular weight: 17.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.715
IPC2_protein 3.694
IPC_protein 3.681
Toseland 3.465
ProMoST 3.821
Dawson 3.668
Bjellqvist 3.872
Wikipedia 3.605
Rodwell 3.503
Grimsley 3.376
Solomon 3.656
Lehninger 3.617
Nozaki 3.795
DTASelect 4.012
Thurlkill 3.528
EMBOSS 3.617
Sillero 3.795
Patrickios 0.947
IPC_peptide 3.656
IPC2_peptide 3.77
IPC2.peptide.svr19 3.752
Protein with the highest isoelectric point:
>tr|A0A4U1B7S3|A0A4U1B7S3_9GAMM Hsp20 family protein OS=Thalassotalea mangrovi OX=2572245 GN=E8M12_05225 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.46 RR12 pKa = 11.84 KK13 pKa = 9.37 RR14 pKa = 11.84 NHH16 pKa = 5.37 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.33 NGRR28 pKa = 11.84 AVIARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.46 GRR39 pKa = 11.84 AKK41 pKa = 10.69 LSAA44 pKa = 3.92
Molecular weight: 5.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.676
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.398
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3157
0
3157
1056890
25
1762
334.8
37.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.657 ± 0.046
1.011 ± 0.015
5.873 ± 0.036
6.013 ± 0.035
4.298 ± 0.03
6.852 ± 0.038
2.224 ± 0.023
6.445 ± 0.033
5.079 ± 0.037
10.212 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.535 ± 0.018
4.446 ± 0.026
3.931 ± 0.027
5.135 ± 0.046
4.536 ± 0.031
6.379 ± 0.029
5.141 ± 0.028
6.772 ± 0.038
1.252 ± 0.015
3.208 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here