Leptosomus discolor (Madagascar cuckoo roller) (Cuculus discolor)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Sauropsida; Sauria; Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9574 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A091PCX1|A0A091PCX1_LEPDC Putative G-protein coupled receptor 83 (Fragment) OS=Leptosomus discolor OX=188344 GN=N330_08104 PE=4 SV=1
MM1 pKa = 6.48TTGDD5 pKa = 4.55ALDD8 pKa = 3.61SCIAAEE14 pKa = 4.34LRR16 pKa = 11.84LDD18 pKa = 3.65SLYY21 pKa = 10.87LQGMDD26 pKa = 3.15SHH28 pKa = 6.95KK29 pKa = 11.25ADD31 pKa = 3.95VLDD34 pKa = 3.89VLPKK38 pKa = 10.26QEE40 pKa = 4.18IQSSEE45 pKa = 4.06CQAAASDD52 pKa = 4.42AEE54 pKa = 4.4VLPTARR60 pKa = 11.84GFLGHH65 pKa = 6.41CTPEE69 pKa = 4.08VLSANAAQTDD79 pKa = 3.6AAEE82 pKa = 4.72CEE84 pKa = 4.35VGLPDD89 pKa = 4.38TYY91 pKa = 11.28LSPTADD97 pKa = 3.3SCDD100 pKa = 3.88NISLATTGKK109 pKa = 10.47GDD111 pKa = 3.75LPHH114 pKa = 6.84SVVYY118 pKa = 10.59QNEE121 pKa = 3.85EE122 pKa = 4.34GKK124 pKa = 9.57WVTDD128 pKa = 3.51LAYY131 pKa = 9.07YY132 pKa = 10.17TSFDD136 pKa = 3.77EE137 pKa = 4.71EE138 pKa = 3.89QDD140 pKa = 3.93LNLSEE145 pKa = 4.94DD146 pKa = 3.61DD147 pKa = 4.51KK148 pKa = 11.63INEE151 pKa = 4.13EE152 pKa = 4.48FVTT155 pKa = 4.12

Molecular weight: 16.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A091Q182|A0A091Q182_LEPDC Ankyrin-3 (Fragment) OS=Leptosomus discolor OX=188344 GN=N330_05002 PE=4 SV=1
HHH2 pKa = 7.52AGPTAATAVPVVPVRR17 pKa = 11.84VRR19 pKa = 11.84AARR22 pKa = 11.84ARR24 pKa = 11.84TVRR27 pKa = 11.84STIAIQTPVAQGGTPMAIQTPIAQGGTPIAVQTPIAQG

Molecular weight: 6.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9574

0

9574

4034616

32

4971

421.4

47.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.384 ± 0.019

2.263 ± 0.024

5.064 ± 0.017

7.155 ± 0.032

3.921 ± 0.02

5.885 ± 0.034

2.528 ± 0.013

4.973 ± 0.018

6.435 ± 0.027

9.654 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.231 ± 0.01

4.171 ± 0.019

5.298 ± 0.03

4.661 ± 0.023

5.199 ± 0.02

8.231 ± 0.038

5.411 ± 0.02

6.338 ± 0.02

1.188 ± 0.01

3.001 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski