Nostoc flagelliforme CCNUN1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Nostocales; Nostocaceae; Nostoc; Nostoc flagelliforme

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10244 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K8T0F6|A0A2K8T0F6_9NOSO Putative transposase YdaD OS=Nostoc flagelliforme CCNUN1 OX=2038116 GN=COO91_07153 PE=4 SV=1
MM1 pKa = 7.32TNIIGSKK8 pKa = 10.92GNDD11 pKa = 3.19TLVGSEE17 pKa = 4.77LADD20 pKa = 4.19TINGLAGNDD29 pKa = 3.94TITATNGNDD38 pKa = 3.19TLTGGGGKK46 pKa = 10.15DD47 pKa = 2.7KK48 pKa = 11.16FKK50 pKa = 11.0FVDD53 pKa = 3.72VFNGTDD59 pKa = 3.39TITDD63 pKa = 4.18FGGVGKK69 pKa = 8.64GTNPTAAVTAEE80 pKa = 3.55VDD82 pKa = 3.42TLKK85 pKa = 11.06FKK87 pKa = 10.9GYY89 pKa = 10.93DD90 pKa = 3.24FFRR93 pKa = 11.84AEE95 pKa = 4.5NLLLTQNGSNLEE107 pKa = 3.93IAFEE111 pKa = 4.51GVGEE115 pKa = 4.31TIVILEE121 pKa = 4.02NFKK124 pKa = 11.28LEE126 pKa = 3.98NLEE129 pKa = 3.99NQKK132 pKa = 11.15ASGATPALGNILFNEE147 pKa = 4.04QTTIIDD153 pKa = 3.64SFDD156 pKa = 3.45VFDD159 pKa = 5.33ANSTEE164 pKa = 4.12TKK166 pKa = 10.51LGIKK170 pKa = 8.81NTVTFLNDD178 pKa = 3.6LSNNITGLDD187 pKa = 3.68DD188 pKa = 4.53SDD190 pKa = 5.48DD191 pKa = 3.87VVNGQGGNDD200 pKa = 3.9KK201 pKa = 10.66IDD203 pKa = 3.71GKK205 pKa = 10.99SGNDD209 pKa = 3.6LLRR212 pKa = 11.84GGTGNDD218 pKa = 3.47TLIGGAGKK226 pKa = 8.59DD227 pKa = 3.5TLIGSTGNNSLLGGAGDD244 pKa = 4.17DD245 pKa = 3.73VLNAEE250 pKa = 4.83DD251 pKa = 3.63STGNSTLNGGAGKK264 pKa = 10.27DD265 pKa = 3.72YY266 pKa = 11.19LSVEE270 pKa = 4.14GSTGDD275 pKa = 3.37NLLFGGDD282 pKa = 3.87GNDD285 pKa = 3.53SFSLRR290 pKa = 11.84ASSTAPSFLVTQTVDD305 pKa = 2.83GGKK308 pKa = 10.65GDD310 pKa = 5.29DD311 pKa = 4.36FLQAYY316 pKa = 6.35YY317 pKa = 10.69TNATGRR323 pKa = 11.84MTSTFNATTNIGSITAGTNRR343 pKa = 11.84VNYY346 pKa = 10.19KK347 pKa = 10.28NIEE350 pKa = 3.97RR351 pKa = 11.84LEE353 pKa = 4.08IRR355 pKa = 11.84GTTYY359 pKa = 11.16NDD361 pKa = 4.72LIVGTNGNDD370 pKa = 3.37YY371 pKa = 11.12LFGGSSGNDD380 pKa = 3.07PSYY383 pKa = 11.9GNDD386 pKa = 3.43TIFGGEE392 pKa = 4.24GNDD395 pKa = 4.24DD396 pKa = 3.95LSVDD400 pKa = 3.92YY401 pKa = 9.85STSDD405 pKa = 3.35NILNGGAGHH414 pKa = 7.08DD415 pKa = 3.73QLSATSSTGNNLLSGDD431 pKa = 4.6DD432 pKa = 4.09GNDD435 pKa = 3.36NLSTSGTHH443 pKa = 6.48IDD445 pKa = 3.67YY446 pKa = 11.23DD447 pKa = 3.93GGNTNAASGNNTLNGGAGDD466 pKa = 4.78DD467 pKa = 4.32NLSADD472 pKa = 3.88YY473 pKa = 9.68STGDD477 pKa = 3.45NLLSGGDD484 pKa = 3.97GNDD487 pKa = 3.39SLSASSYY494 pKa = 9.8PSDD497 pKa = 3.56RR498 pKa = 11.84YY499 pKa = 11.17GFDD502 pKa = 2.97TSGNNTLDD510 pKa = 3.49GGAGDD515 pKa = 4.44DD516 pKa = 3.9TLIANYY522 pKa = 10.52SSGDD526 pKa = 3.44NLFFGGDD533 pKa = 3.64GNDD536 pKa = 3.42SLFVSSYY543 pKa = 9.36YY544 pKa = 10.58DD545 pKa = 3.23YY546 pKa = 11.42SGRR549 pKa = 11.84FDD551 pKa = 3.57GAFGNNLLSGGDD563 pKa = 3.83GNDD566 pKa = 3.24SFSLVLYY573 pKa = 7.18TSPSDD578 pKa = 4.65LVTQTVDD585 pKa = 3.02GGTGDD590 pKa = 4.62DD591 pKa = 4.27LLEE594 pKa = 3.81VSYY597 pKa = 10.52IYY599 pKa = 8.47TTEE602 pKa = 5.14GITSTFDD609 pKa = 3.0AATNTGRR616 pKa = 11.84VTAGTSHH623 pKa = 5.08VTYY626 pKa = 11.04KK627 pKa = 10.86NIEE630 pKa = 3.98GLNISGTEE638 pKa = 3.86YY639 pKa = 11.15DD640 pKa = 4.1DD641 pKa = 6.65LIVGSNGNDD650 pKa = 3.52KK651 pKa = 10.66IDD653 pKa = 3.76GSVSGKK659 pKa = 8.76DD660 pKa = 3.47TIDD663 pKa = 3.5GGTGDD668 pKa = 5.03DD669 pKa = 4.02YY670 pKa = 11.94LSFSCYY676 pKa = 10.2SATQSITSTFDD687 pKa = 2.84AATNTGRR694 pKa = 11.84VTAGTSHH701 pKa = 5.08VTYY704 pKa = 11.09KK705 pKa = 10.86NIEE708 pKa = 3.83QLNIYY713 pKa = 8.28GTDD716 pKa = 3.58YY717 pKa = 11.68DD718 pKa = 4.26DD719 pKa = 7.04LIVGSNGNDD728 pKa = 3.31SLHH731 pKa = 6.65GGNSGNDD738 pKa = 3.57TIDD741 pKa = 3.37GGKK744 pKa = 10.57GDD746 pKa = 5.43DD747 pKa = 4.99LLSLYY752 pKa = 10.76NYY754 pKa = 6.93TTSDD758 pKa = 3.97GITSTFNAATNTGRR772 pKa = 11.84VTAGTSHH779 pKa = 5.08VTYY782 pKa = 11.09KK783 pKa = 10.86NIEE786 pKa = 3.88QLNISGTEE794 pKa = 3.8YY795 pKa = 11.15DD796 pKa = 4.1DD797 pKa = 6.65LIVGSNGNDD806 pKa = 3.79TINGAGGNNTIFGGAGKK823 pKa = 10.17DD824 pKa = 3.75YY825 pKa = 11.34LSADD829 pKa = 3.51YY830 pKa = 11.67SNGNNLLSGDD840 pKa = 4.74DD841 pKa = 4.67GNDD844 pKa = 3.21SLNISGHH851 pKa = 5.17YY852 pKa = 9.63NDD854 pKa = 4.67YY855 pKa = 10.85RR856 pKa = 11.84GEE858 pKa = 4.17FYY860 pKa = 11.18LDD862 pKa = 3.05AVSGNNTLNGGAGDD876 pKa = 4.58DD877 pKa = 3.79NLVTNYY883 pKa = 10.67SSGNNLLSGGDD894 pKa = 3.63GNDD897 pKa = 3.42YY898 pKa = 11.12LSASGSASLYY908 pKa = 10.15SSYY911 pKa = 11.34GVYY914 pKa = 9.2TVSGNNTLNGGAGNDD929 pKa = 3.6RR930 pKa = 11.84LIVDD934 pKa = 4.29YY935 pKa = 10.22STGDD939 pKa = 3.41NLLSGGDD946 pKa = 3.92GNDD949 pKa = 3.03SLTAFGASGKK959 pKa = 8.37NTLTGGNGNDD969 pKa = 3.58TLTGGKK975 pKa = 10.55GNDD978 pKa = 3.62SLIGGAGTDD987 pKa = 3.52TFALNSFNEE996 pKa = 5.19GIDD999 pKa = 3.49TIYY1002 pKa = 11.11DD1003 pKa = 3.76FNATNEE1009 pKa = 4.49LIQISAAGFGGGLSSGSLEE1028 pKa = 4.13TSHH1031 pKa = 6.51FTLGTSATTSEE1042 pKa = 3.85EE1043 pKa = 3.77RR1044 pKa = 11.84FIYY1047 pKa = 10.64NSATGGLFFDD1057 pKa = 5.26LDD1059 pKa = 3.95GSASGSTQVQFAQLSAGLSVTEE1081 pKa = 4.07NNFVVVV1087 pKa = 4.06

Molecular weight: 111.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K8SHK8|A0A2K8SHK8_9NOSO Glucan-binding domain OS=Nostoc flagelliforme CCNUN1 OX=2038116 GN=COO91_00759 PE=4 SV=1
MM1 pKa = 7.44SLFARR6 pKa = 11.84QLNLFARR13 pKa = 11.84QLSLFARR20 pKa = 11.84QLSLFARR27 pKa = 11.84QLSLFARR34 pKa = 11.84QLSLFTRR41 pKa = 11.84QLSLFTRR48 pKa = 4.37

Molecular weight: 5.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10244

0

10244

2470546

29

10889

241.2

26.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.039 ± 0.031

1.008 ± 0.01

4.839 ± 0.023

6.274 ± 0.031

3.964 ± 0.019

6.629 ± 0.037

1.784 ± 0.013

6.724 ± 0.023

5.076 ± 0.03

10.878 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.815 ± 0.014

4.602 ± 0.033

4.594 ± 0.021

5.323 ± 0.028

5.096 ± 0.023

6.712 ± 0.025

5.717 ± 0.028

6.504 ± 0.022

1.408 ± 0.012

3.014 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski