Hydrogenophilus thermoluteolus (Pseudomonas hydrogenothermophila)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Hydrogenophilalia; Hydrogenophilales; Hydrogenophilaceae; Hydrogenophilus

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2138 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z6DWR6|A0A2Z6DWR6_HYDTE Sulfite exporter TauE/SafE family protein OS=Hydrogenophilus thermoluteolus OX=297 GN=HPTL_0638 PE=4 SV=1
MM1 pKa = 7.4SEE3 pKa = 4.24AVVDD7 pKa = 4.15DD8 pKa = 3.91VLVFTDD14 pKa = 3.91AAAAKK19 pKa = 9.44VRR21 pKa = 11.84EE22 pKa = 4.48LIEE25 pKa = 4.5EE26 pKa = 4.2EE27 pKa = 4.41GNPNLKK33 pKa = 10.3LRR35 pKa = 11.84VFITGGGCSGFQYY48 pKa = 10.93GFTFDD53 pKa = 4.82EE54 pKa = 4.76EE55 pKa = 4.54VADD58 pKa = 4.82DD59 pKa = 3.87DD60 pKa = 4.53TVVEE64 pKa = 4.41KK65 pKa = 11.21GGVTLLIDD73 pKa = 3.69SMSYY77 pKa = 10.23QYY79 pKa = 11.55LVGAEE84 pKa = 3.67IDD86 pKa = 3.85YY87 pKa = 11.6VEE89 pKa = 5.84DD90 pKa = 3.24INGAQFVIRR99 pKa = 11.84NPNAVTTCGCGSSFSVV115 pKa = 3.54

Molecular weight: 12.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z6E196|A0A2Z6E196_HYDTE Integrase OS=Hydrogenophilus thermoluteolus OX=297 GN=HPTL_P024 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.25QPSKK9 pKa = 9.09VRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.18RR14 pKa = 11.84THH16 pKa = 5.72GFLVRR21 pKa = 11.84MRR23 pKa = 11.84SRR25 pKa = 11.84GGRR28 pKa = 11.84SVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.67GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2138

0

2138

712812

39

1931

333.4

36.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.994 ± 0.077

0.918 ± 0.019

4.851 ± 0.034

6.346 ± 0.054

3.624 ± 0.03

7.321 ± 0.053

2.392 ± 0.029

4.634 ± 0.037

3.077 ± 0.041

10.864 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.898 ± 0.025

2.344 ± 0.027

5.806 ± 0.044

3.683 ± 0.037

7.589 ± 0.051

4.192 ± 0.031

5.592 ± 0.035

7.722 ± 0.054

1.911 ± 0.031

2.243 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski