Hyphomonas pacifica

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomonadales; Hyphomonadaceae; Hyphomonas

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3301 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A062U1F5|A0A062U1F5_9PROT Uncharacterized protein OS=Hyphomonas pacifica OX=1280941 GN=HY11_03775 PE=4 SV=1
MM1 pKa = 7.29RR2 pKa = 11.84RR3 pKa = 11.84GWILAAAGLLAACGEE18 pKa = 4.39RR19 pKa = 11.84VEE21 pKa = 5.11EE22 pKa = 4.09PVEE25 pKa = 3.93PDD27 pKa = 3.23VTVVEE32 pKa = 4.54APDD35 pKa = 4.91PIEE38 pKa = 4.38TPAEE42 pKa = 3.85IAKK45 pKa = 10.41SDD47 pKa = 4.05GEE49 pKa = 4.1TDD51 pKa = 3.62YY52 pKa = 11.45QAVSGYY58 pKa = 10.89GEE60 pKa = 4.16GWYY63 pKa = 10.33VSPGWPGEE71 pKa = 4.16YY72 pKa = 9.43PAGFAVLDD80 pKa = 4.06PGVVVNGWSQPNSGVPADD98 pKa = 4.79LEE100 pKa = 4.61CPLPQYY106 pKa = 11.59ANYY109 pKa = 9.87QLWNRR114 pKa = 11.84DD115 pKa = 3.24RR116 pKa = 11.84VEE118 pKa = 4.98KK119 pKa = 10.65DD120 pKa = 3.14DD121 pKa = 5.55LDD123 pKa = 4.06FFVATKK129 pKa = 8.42TFPVTLSQDD138 pKa = 2.8ASIEE142 pKa = 4.25YY143 pKa = 10.74VSDD146 pKa = 3.46TGIKK150 pKa = 9.28TLDD153 pKa = 4.12LKK155 pKa = 10.42TGDD158 pKa = 3.16QLTYY162 pKa = 10.8LRR164 pKa = 11.84YY165 pKa = 9.86LGEE168 pKa = 4.25GFTIVSFGGEE178 pKa = 3.68EE179 pKa = 3.92YY180 pKa = 10.59DD181 pKa = 3.76INEE184 pKa = 4.14AEE186 pKa = 4.41LRR188 pKa = 11.84EE189 pKa = 3.97ISDD192 pKa = 3.48ISSLPAPTEE201 pKa = 4.08DD202 pKa = 2.4QWVNVEE208 pKa = 4.5CEE210 pKa = 4.12DD211 pKa = 5.05GSRR214 pKa = 11.84AWLLYY219 pKa = 9.65STMVDD224 pKa = 3.37LPGVVASPIMGYY236 pKa = 10.91GEE238 pKa = 4.72ASDD241 pKa = 3.95IAPDD245 pKa = 3.57DD246 pKa = 3.83VAEE249 pKa = 4.34VRR251 pKa = 11.84AGAAAMANAYY261 pKa = 9.85EE262 pKa = 4.8GIEE265 pKa = 4.19PEE267 pKa = 4.35AALPDD272 pKa = 3.75QEE274 pKa = 4.33

Molecular weight: 29.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A062TZ82|A0A062TZ82_9PROT NADPH:quinone oxidoreductase OS=Hyphomonas pacifica OX=1280941 GN=HY11_10255 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.17RR14 pKa = 11.84THH16 pKa = 5.98GFRR19 pKa = 11.84DD20 pKa = 3.34RR21 pKa = 11.84MSTKK25 pKa = 10.29NGRR28 pKa = 11.84KK29 pKa = 8.58VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.15GRR39 pKa = 11.84KK40 pKa = 8.87SLSAA44 pKa = 3.86

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3301

0

3301

1037903

29

2510

314.4

34.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.651 ± 0.062

0.839 ± 0.015

6.189 ± 0.031

6.563 ± 0.043

3.669 ± 0.028

8.371 ± 0.042

1.929 ± 0.022

5.268 ± 0.034

3.829 ± 0.032

9.698 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.705 ± 0.023

2.776 ± 0.023

4.987 ± 0.029

3.227 ± 0.024

6.403 ± 0.044

5.739 ± 0.034

5.442 ± 0.032

6.881 ± 0.037

1.386 ± 0.019

2.444 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski