Amino acid dipepetide frequency for Escherichia phage ESSI2_ev239

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.867AlaAla: 10.867 ± 1.539
0.224AlaCys: 0.224 ± 0.184
4.033AlaAsp: 4.033 ± 0.777
6.274AlaGlu: 6.274 ± 0.853
4.257AlaPhe: 4.257 ± 0.641
8.514AlaGly: 8.514 ± 1.111
2.129AlaHis: 2.129 ± 0.591
5.826AlaIle: 5.826 ± 1.098
5.041AlaLys: 5.041 ± 0.854
8.963AlaLeu: 8.963 ± 1.414
2.913AlaMet: 2.913 ± 0.658
3.137AlaAsn: 3.137 ± 0.523
2.241AlaPro: 2.241 ± 0.538
4.257AlaGln: 4.257 ± 0.541
4.929AlaArg: 4.929 ± 0.596
6.498AlaSer: 6.498 ± 1.167
5.266AlaThr: 5.266 ± 0.704
7.618AlaVal: 7.618 ± 0.8
1.568AlaTrp: 1.568 ± 0.464
2.241AlaTyr: 2.241 ± 0.455
0.0AlaXaa: 0.0 ± 0.0
Cys
0.896CysAla: 0.896 ± 0.367
0.112CysCys: 0.112 ± 0.11
0.56CysAsp: 0.56 ± 0.213
0.336CysGlu: 0.336 ± 0.202
0.224CysPhe: 0.224 ± 0.141
1.008CysGly: 1.008 ± 0.394
0.112CysHis: 0.112 ± 0.106
0.448CysIle: 0.448 ± 0.222
0.112CysLys: 0.112 ± 0.102
0.336CysLeu: 0.336 ± 0.215
0.0CysMet: 0.0 ± 0.0
0.336CysAsn: 0.336 ± 0.185
0.56CysPro: 0.56 ± 0.255
0.56CysGln: 0.56 ± 0.303
0.448CysArg: 0.448 ± 0.201
0.56CysSer: 0.56 ± 0.26
0.336CysThr: 0.336 ± 0.192
0.336CysVal: 0.336 ± 0.183
0.448CysTrp: 0.448 ± 0.242
0.224CysTyr: 0.224 ± 0.13
0.0CysXaa: 0.0 ± 0.0
Asp
4.369AspAla: 4.369 ± 0.972
0.224AspCys: 0.224 ± 0.186
2.577AspAsp: 2.577 ± 0.711
4.033AspGlu: 4.033 ± 0.651
2.913AspPhe: 2.913 ± 0.62
5.153AspGly: 5.153 ± 0.723
0.672AspHis: 0.672 ± 0.232
4.593AspIle: 4.593 ± 0.666
3.025AspLys: 3.025 ± 0.702
3.361AspLeu: 3.361 ± 0.539
1.008AspMet: 1.008 ± 0.334
1.568AspAsn: 1.568 ± 0.319
2.241AspPro: 2.241 ± 0.536
1.12AspGln: 1.12 ± 0.276
1.68AspArg: 1.68 ± 0.36
2.913AspSer: 2.913 ± 0.608
3.249AspThr: 3.249 ± 0.767
4.593AspVal: 4.593 ± 0.94
1.12AspTrp: 1.12 ± 0.39
2.577AspTyr: 2.577 ± 0.648
0.0AspXaa: 0.0 ± 0.0
Glu
4.817GluAla: 4.817 ± 0.918
0.224GluCys: 0.224 ± 0.27
3.249GluAsp: 3.249 ± 0.877
3.585GluGlu: 3.585 ± 0.792
2.913GluPhe: 2.913 ± 0.578
4.481GluGly: 4.481 ± 0.606
1.008GluHis: 1.008 ± 0.338
4.257GluIle: 4.257 ± 0.982
4.257GluLys: 4.257 ± 0.846
7.394GluLeu: 7.394 ± 0.978
2.017GluMet: 2.017 ± 0.467
3.473GluAsn: 3.473 ± 0.735
2.353GluPro: 2.353 ± 0.551
2.801GluGln: 2.801 ± 0.5
4.033GluArg: 4.033 ± 1.236
3.473GluSer: 3.473 ± 0.69
4.481GluThr: 4.481 ± 0.878
4.481GluVal: 4.481 ± 0.736
0.896GluTrp: 0.896 ± 0.313
1.344GluTyr: 1.344 ± 0.359
0.0GluXaa: 0.0 ± 0.0
Phe
3.585PheAla: 3.585 ± 0.446
0.224PheCys: 0.224 ± 0.16
3.585PheAsp: 3.585 ± 0.661
2.577PheGlu: 2.577 ± 0.631
1.232PhePhe: 1.232 ± 0.374
2.689PheGly: 2.689 ± 0.586
0.224PheHis: 0.224 ± 0.141
2.913PheIle: 2.913 ± 0.7
1.68PheLys: 1.68 ± 0.475
3.025PheLeu: 3.025 ± 0.687
1.008PheMet: 1.008 ± 0.325
2.353PheAsn: 2.353 ± 0.462
0.896PhePro: 0.896 ± 0.227
1.12PheGln: 1.12 ± 0.423
2.241PheArg: 2.241 ± 0.498
3.921PheSer: 3.921 ± 0.879
3.473PheThr: 3.473 ± 0.739
2.689PheVal: 2.689 ± 0.56
1.12PheTrp: 1.12 ± 0.405
0.896PheTyr: 0.896 ± 0.306
0.0PheXaa: 0.0 ± 0.0
Gly
5.378GlyAla: 5.378 ± 0.978
0.56GlyCys: 0.56 ± 0.241
4.369GlyAsp: 4.369 ± 0.927
6.274GlyGlu: 6.274 ± 0.827
3.249GlyPhe: 3.249 ± 0.622
4.257GlyGly: 4.257 ± 0.807
1.344GlyHis: 1.344 ± 0.401
4.817GlyIle: 4.817 ± 0.777
4.593GlyLys: 4.593 ± 0.836
4.705GlyLeu: 4.705 ± 0.811
2.353GlyMet: 2.353 ± 0.45
5.041GlyAsn: 5.041 ± 0.956
0.224GlyPro: 0.224 ± 0.135
4.481GlyGln: 4.481 ± 0.561
4.257GlyArg: 4.257 ± 0.788
3.697GlySer: 3.697 ± 0.637
4.817GlyThr: 4.817 ± 0.806
5.602GlyVal: 5.602 ± 0.97
1.568GlyTrp: 1.568 ± 0.454
2.241GlyTyr: 2.241 ± 0.532
0.0GlyXaa: 0.0 ± 0.0
His
1.456HisAla: 1.456 ± 0.384
0.0HisCys: 0.0 ± 0.0
0.672HisAsp: 0.672 ± 0.239
0.896HisGlu: 0.896 ± 0.361
0.784HisPhe: 0.784 ± 0.284
1.232HisGly: 1.232 ± 0.44
0.112HisHis: 0.112 ± 0.109
1.232HisIle: 1.232 ± 0.363
0.672HisLys: 0.672 ± 0.278
1.793HisLeu: 1.793 ± 0.457
0.224HisMet: 0.224 ± 0.172
1.232HisAsn: 1.232 ± 0.333
0.784HisPro: 0.784 ± 0.296
0.672HisGln: 0.672 ± 0.31
0.784HisArg: 0.784 ± 0.259
1.232HisSer: 1.232 ± 0.341
2.129HisThr: 2.129 ± 0.756
0.448HisVal: 0.448 ± 0.237
0.672HisTrp: 0.672 ± 0.262
0.784HisTyr: 0.784 ± 0.308
0.0HisXaa: 0.0 ± 0.0
Ile
6.834IleAla: 6.834 ± 0.856
0.672IleCys: 0.672 ± 0.389
3.249IleAsp: 3.249 ± 0.585
3.921IleGlu: 3.921 ± 0.707
2.577IlePhe: 2.577 ± 0.578
3.137IleGly: 3.137 ± 0.68
1.12IleHis: 1.12 ± 0.317
2.801IleIle: 2.801 ± 0.753
3.921IleLys: 3.921 ± 0.643
2.577IleLeu: 2.577 ± 0.466
1.344IleMet: 1.344 ± 0.395
2.353IleAsn: 2.353 ± 0.452
3.361IlePro: 3.361 ± 0.681
2.129IleGln: 2.129 ± 0.476
2.801IleArg: 2.801 ± 0.714
3.809IleSer: 3.809 ± 1.012
4.369IleThr: 4.369 ± 0.679
2.913IleVal: 2.913 ± 0.558
0.56IleTrp: 0.56 ± 0.32
2.017IleTyr: 2.017 ± 0.682
0.0IleXaa: 0.0 ± 0.0
Lys
5.602LysAla: 5.602 ± 0.764
0.896LysCys: 0.896 ± 0.32
2.017LysAsp: 2.017 ± 0.434
3.025LysGlu: 3.025 ± 0.825
1.68LysPhe: 1.68 ± 0.41
4.593LysGly: 4.593 ± 0.739
1.456LysHis: 1.456 ± 0.47
2.129LysIle: 2.129 ± 0.563
3.249LysLys: 3.249 ± 0.595
3.921LysLeu: 3.921 ± 0.846
1.344LysMet: 1.344 ± 0.32
2.129LysAsn: 2.129 ± 0.56
3.249LysPro: 3.249 ± 0.56
1.793LysGln: 1.793 ± 0.45
4.257LysArg: 4.257 ± 0.726
4.257LysSer: 4.257 ± 0.784
3.697LysThr: 3.697 ± 0.538
2.577LysVal: 2.577 ± 0.566
0.448LysTrp: 0.448 ± 0.284
2.465LysTyr: 2.465 ± 0.582
0.0LysXaa: 0.0 ± 0.0
Leu
9.971LeuAla: 9.971 ± 1.126
0.896LeuCys: 0.896 ± 0.279
5.49LeuAsp: 5.49 ± 0.812
4.369LeuGlu: 4.369 ± 0.813
2.801LeuPhe: 2.801 ± 0.628
5.041LeuGly: 5.041 ± 0.735
1.12LeuHis: 1.12 ± 0.34
4.145LeuIle: 4.145 ± 0.67
5.041LeuLys: 5.041 ± 1.069
8.963LeuLeu: 8.963 ± 1.087
2.465LeuMet: 2.465 ± 0.689
4.481LeuAsn: 4.481 ± 0.593
4.705LeuPro: 4.705 ± 0.719
3.137LeuGln: 3.137 ± 0.627
5.49LeuArg: 5.49 ± 0.821
5.153LeuSer: 5.153 ± 0.817
5.714LeuThr: 5.714 ± 0.704
5.602LeuVal: 5.602 ± 0.716
0.896LeuTrp: 0.896 ± 0.227
3.697LeuTyr: 3.697 ± 0.901
0.0LeuXaa: 0.0 ± 0.0
Met
2.577MetAla: 2.577 ± 0.719
0.224MetCys: 0.224 ± 0.16
0.896MetAsp: 0.896 ± 0.376
1.568MetGlu: 1.568 ± 0.402
0.896MetPhe: 0.896 ± 0.352
1.456MetGly: 1.456 ± 0.494
0.224MetHis: 0.224 ± 0.159
1.12MetIle: 1.12 ± 0.361
1.568MetLys: 1.568 ± 0.352
1.905MetLeu: 1.905 ± 0.406
0.672MetMet: 0.672 ± 0.28
1.344MetAsn: 1.344 ± 0.338
1.232MetPro: 1.232 ± 0.366
1.12MetGln: 1.12 ± 0.267
1.456MetArg: 1.456 ± 0.393
2.129MetSer: 2.129 ± 0.51
2.353MetThr: 2.353 ± 0.68
1.232MetVal: 1.232 ± 0.427
0.56MetTrp: 0.56 ± 0.25
0.896MetTyr: 0.896 ± 0.325
0.0MetXaa: 0.0 ± 0.0
Asn
4.145AsnAla: 4.145 ± 0.542
0.56AsnCys: 0.56 ± 0.241
2.129AsnAsp: 2.129 ± 0.396
2.801AsnGlu: 2.801 ± 0.549
2.017AsnPhe: 2.017 ± 0.506
4.481AsnGly: 4.481 ± 0.873
0.784AsnHis: 0.784 ± 0.355
2.913AsnIle: 2.913 ± 0.71
2.353AsnLys: 2.353 ± 0.467
3.697AsnLeu: 3.697 ± 0.567
2.017AsnMet: 2.017 ± 0.506
1.68AsnAsn: 1.68 ± 0.532
1.905AsnPro: 1.905 ± 0.49
1.68AsnGln: 1.68 ± 0.431
2.801AsnArg: 2.801 ± 0.779
2.017AsnSer: 2.017 ± 0.567
1.793AsnThr: 1.793 ± 0.443
1.68AsnVal: 1.68 ± 0.451
0.784AsnTrp: 0.784 ± 0.284
1.568AsnTyr: 1.568 ± 0.365
0.0AsnXaa: 0.0 ± 0.0
Pro
4.481ProAla: 4.481 ± 0.813
0.112ProCys: 0.112 ± 0.125
3.137ProAsp: 3.137 ± 0.628
3.697ProGlu: 3.697 ± 0.838
1.68ProPhe: 1.68 ± 0.448
2.801ProGly: 2.801 ± 0.738
0.672ProHis: 0.672 ± 0.266
1.008ProIle: 1.008 ± 0.434
2.017ProLys: 2.017 ± 0.432
3.025ProLeu: 3.025 ± 0.567
0.784ProMet: 0.784 ± 0.265
1.12ProAsn: 1.12 ± 0.304
2.017ProPro: 2.017 ± 0.427
1.12ProGln: 1.12 ± 0.337
2.129ProArg: 2.129 ± 0.382
1.905ProSer: 1.905 ± 0.369
1.905ProThr: 1.905 ± 0.433
4.369ProVal: 4.369 ± 0.797
0.448ProTrp: 0.448 ± 0.172
0.896ProTyr: 0.896 ± 0.324
0.0ProXaa: 0.0 ± 0.0
Gln
4.033GlnAla: 4.033 ± 0.571
0.336GlnCys: 0.336 ± 0.178
1.905GlnAsp: 1.905 ± 0.447
2.241GlnGlu: 2.241 ± 0.477
1.232GlnPhe: 1.232 ± 0.305
2.465GlnGly: 2.465 ± 0.453
1.008GlnHis: 1.008 ± 0.347
3.025GlnIle: 3.025 ± 0.567
1.232GlnLys: 1.232 ± 0.268
4.817GlnLeu: 4.817 ± 0.859
0.56GlnMet: 0.56 ± 0.259
1.456GlnAsn: 1.456 ± 0.322
1.12GlnPro: 1.12 ± 0.26
3.137GlnGln: 3.137 ± 0.728
3.585GlnArg: 3.585 ± 0.764
2.801GlnSer: 2.801 ± 0.574
3.585GlnThr: 3.585 ± 0.814
1.905GlnVal: 1.905 ± 0.467
0.56GlnTrp: 0.56 ± 0.218
1.232GlnTyr: 1.232 ± 0.398
0.0GlnXaa: 0.0 ± 0.0
Arg
4.593ArgAla: 4.593 ± 0.906
0.56ArgCys: 0.56 ± 0.301
1.793ArgAsp: 1.793 ± 0.452
3.921ArgGlu: 3.921 ± 0.887
2.129ArgPhe: 2.129 ± 0.503
3.137ArgGly: 3.137 ± 0.835
1.344ArgHis: 1.344 ± 0.529
3.697ArgIle: 3.697 ± 0.806
3.025ArgLys: 3.025 ± 0.792
6.162ArgLeu: 6.162 ± 0.715
1.793ArgMet: 1.793 ± 0.338
2.465ArgAsn: 2.465 ± 0.608
1.68ArgPro: 1.68 ± 0.506
3.025ArgGln: 3.025 ± 0.721
3.137ArgArg: 3.137 ± 0.878
2.801ArgSer: 2.801 ± 0.491
2.801ArgThr: 2.801 ± 0.588
3.137ArgVal: 3.137 ± 0.646
1.232ArgTrp: 1.232 ± 0.308
2.241ArgTyr: 2.241 ± 0.632
0.0ArgXaa: 0.0 ± 0.0
Ser
6.274SerAla: 6.274 ± 0.819
0.56SerCys: 0.56 ± 0.27
3.697SerAsp: 3.697 ± 0.724
3.809SerGlu: 3.809 ± 0.712
2.913SerPhe: 2.913 ± 0.519
5.378SerGly: 5.378 ± 0.802
1.456SerHis: 1.456 ± 0.446
3.025SerIle: 3.025 ± 0.45
3.025SerLys: 3.025 ± 0.656
6.274SerLeu: 6.274 ± 0.689
0.784SerMet: 0.784 ± 0.304
1.344SerAsn: 1.344 ± 0.421
2.241SerPro: 2.241 ± 0.596
2.577SerGln: 2.577 ± 0.538
2.353SerArg: 2.353 ± 0.437
2.465SerSer: 2.465 ± 0.631
3.921SerThr: 3.921 ± 0.663
4.257SerVal: 4.257 ± 0.746
1.344SerTrp: 1.344 ± 0.403
1.008SerTyr: 1.008 ± 0.313
0.0SerXaa: 0.0 ± 0.0
Thr
6.61ThrAla: 6.61 ± 0.855
0.448ThrCys: 0.448 ± 0.257
3.809ThrAsp: 3.809 ± 0.717
5.041ThrGlu: 5.041 ± 0.699
2.801ThrPhe: 2.801 ± 0.641
6.834ThrGly: 6.834 ± 0.771
0.896ThrHis: 0.896 ± 0.286
3.137ThrIle: 3.137 ± 0.542
2.689ThrLys: 2.689 ± 0.613
7.058ThrLeu: 7.058 ± 1.093
1.232ThrMet: 1.232 ± 0.41
2.801ThrAsn: 2.801 ± 0.464
2.353ThrPro: 2.353 ± 0.649
2.353ThrGln: 2.353 ± 0.533
2.465ThrArg: 2.465 ± 0.457
3.585ThrSer: 3.585 ± 0.702
3.137ThrThr: 3.137 ± 0.556
4.705ThrVal: 4.705 ± 0.935
1.232ThrTrp: 1.232 ± 0.32
1.008ThrTyr: 1.008 ± 0.271
0.0ThrXaa: 0.0 ± 0.0
Val
7.17ValAla: 7.17 ± 0.699
0.672ValCys: 0.672 ± 0.342
3.697ValAsp: 3.697 ± 0.582
4.145ValGlu: 4.145 ± 0.756
2.689ValPhe: 2.689 ± 0.545
3.809ValGly: 3.809 ± 0.824
1.12ValHis: 1.12 ± 0.424
2.801ValIle: 2.801 ± 0.58
5.041ValLys: 5.041 ± 0.782
5.266ValLeu: 5.266 ± 0.985
1.68ValMet: 1.68 ± 0.341
3.137ValAsn: 3.137 ± 0.658
3.025ValPro: 3.025 ± 0.666
2.689ValGln: 2.689 ± 0.528
2.913ValArg: 2.913 ± 0.536
3.809ValSer: 3.809 ± 0.695
4.705ValThr: 4.705 ± 0.803
4.593ValVal: 4.593 ± 0.659
1.68ValTrp: 1.68 ± 0.397
1.68ValTyr: 1.68 ± 0.404
0.0ValXaa: 0.0 ± 0.0
Trp
1.456TrpAla: 1.456 ± 0.484
0.112TrpCys: 0.112 ± 0.128
0.896TrpAsp: 0.896 ± 0.313
0.896TrpGlu: 0.896 ± 0.398
0.784TrpPhe: 0.784 ± 0.247
0.896TrpGly: 0.896 ± 0.315
0.336TrpHis: 0.336 ± 0.171
1.12TrpIle: 1.12 ± 0.383
1.456TrpLys: 1.456 ± 0.408
2.465TrpLeu: 2.465 ± 0.491
0.448TrpMet: 0.448 ± 0.222
0.896TrpAsn: 0.896 ± 0.354
1.12TrpPro: 1.12 ± 0.296
1.12TrpGln: 1.12 ± 0.371
1.008TrpArg: 1.008 ± 0.342
0.448TrpSer: 0.448 ± 0.185
0.784TrpThr: 0.784 ± 0.213
1.12TrpVal: 1.12 ± 0.34
0.448TrpTrp: 0.448 ± 0.193
0.56TrpTyr: 0.56 ± 0.202
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.68TyrAla: 1.68 ± 0.359
0.336TyrCys: 0.336 ± 0.265
1.232TyrAsp: 1.232 ± 0.315
2.129TyrGlu: 2.129 ± 0.624
1.68TyrPhe: 1.68 ± 0.63
2.465TyrGly: 2.465 ± 0.636
0.672TyrHis: 0.672 ± 0.277
1.456TyrIle: 1.456 ± 0.47
0.896TyrLys: 0.896 ± 0.363
3.473TyrLeu: 3.473 ± 0.759
0.672TyrMet: 0.672 ± 0.22
1.456TyrAsn: 1.456 ± 0.425
2.017TyrPro: 2.017 ± 0.305
1.344TyrGln: 1.344 ± 0.424
1.905TyrArg: 1.905 ± 0.552
1.232TyrSer: 1.232 ± 0.402
1.68TyrThr: 1.68 ± 0.42
2.465TyrVal: 2.465 ± 0.519
0.784TyrTrp: 0.784 ± 0.272
0.56TyrTyr: 0.56 ± 0.261
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 37 proteins (8927 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski