TM7 phylum sp. oral taxon 353

Taxonomy: cellular organisms; Bacteria; Bacteria incertae sedis; Bacteria candidate phyla; Candidatus Saccharibacteria

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 656 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A563CBQ7|A0A563CBQ7_9BACT Uncharacterized protein (Fragment) OS=TM7 phylum sp. oral taxon 353 OX=713055 GN=EUA75_02610 PE=4 SV=1
MM1 pKa = 7.48NYY3 pKa = 10.16DD4 pKa = 4.37RR5 pKa = 11.84YY6 pKa = 11.16LEE8 pKa = 4.23LQTRR12 pKa = 11.84LEE14 pKa = 4.2WFYY17 pKa = 11.49DD18 pKa = 3.78FHH20 pKa = 8.64PEE22 pKa = 3.97FFDD25 pKa = 6.92DD26 pKa = 5.62ILPEE30 pKa = 4.16QKK32 pKa = 10.53KK33 pKa = 10.19LLQDD37 pKa = 3.34TFLYY41 pKa = 7.76DD42 pKa = 3.81TPDD45 pKa = 3.0EE46 pKa = 4.79SYY48 pKa = 10.91PEE50 pKa = 4.2SLQDD54 pKa = 5.23FYY56 pKa = 11.92DD57 pKa = 3.93KK58 pKa = 11.39NIDD61 pKa = 3.93NRR63 pKa = 11.84PTLQDD68 pKa = 3.6DD69 pKa = 3.54MFLAVDD75 pKa = 4.47ALYY78 pKa = 10.44KK79 pKa = 10.69AAGASSLFDD88 pKa = 4.21DD89 pKa = 3.94NGYY92 pKa = 10.72RR93 pKa = 11.84SLAEE97 pKa = 3.8

Molecular weight: 11.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A563CDV7|A0A563CDV7_9BACT DNA repair protein RecO (Fragment) OS=TM7 phylum sp. oral taxon 353 OX=713055 GN=recO PE=3 SV=1
MM1 pKa = 7.84PKK3 pKa = 10.55LKK5 pKa = 8.66THH7 pKa = 6.88KK8 pKa = 9.69GTAKK12 pKa = 10.41RR13 pKa = 11.84IKK15 pKa = 9.53LTSTGKK21 pKa = 8.57LTRR24 pKa = 11.84QRR26 pKa = 11.84AFGGHH31 pKa = 6.05FLAKK35 pKa = 9.76KK36 pKa = 9.27SKK38 pKa = 9.27SRR40 pKa = 11.84KK41 pKa = 8.29RR42 pKa = 11.84AINTTAKK49 pKa = 9.29VTGSMAKK56 pKa = 9.54NVRR59 pKa = 11.84RR60 pKa = 11.84AIGAA64 pKa = 3.61

Molecular weight: 6.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

656

0

656

183858

42

1339

280.3

31.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.74 ± 0.103

0.736 ± 0.03

6.093 ± 0.088

6.096 ± 0.096

3.751 ± 0.064

6.705 ± 0.1

1.82 ± 0.038

7.003 ± 0.084

6.581 ± 0.106

9.196 ± 0.101

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.326 ± 0.044

4.545 ± 0.081

3.855 ± 0.074

3.876 ± 0.071

5.441 ± 0.08

6.719 ± 0.089

5.381 ± 0.07

6.871 ± 0.095

1.033 ± 0.036

3.233 ± 0.067
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski