Pseudomonas phage UFV-P2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Vicosavirus; Pseudomonas virus UFVP2

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4T216|M4T216_9CAUD Uncharacterized protein OS=Pseudomonas phage UFV-P2 OX=1235661 PE=4 SV=1
MM1 pKa = 7.68GNPVLKK7 pKa = 10.59SVVTGVVVAEE17 pKa = 3.97TLEE20 pKa = 4.21FSRR23 pKa = 11.84GQMWADD29 pKa = 3.36EE30 pKa = 4.28KK31 pKa = 11.42GQVLLCTDD39 pKa = 3.87EE40 pKa = 3.9QTMVVIADD48 pKa = 4.23KK49 pKa = 11.29LPGNIGTIYY58 pKa = 10.57NFYY61 pKa = 11.04DD62 pKa = 3.06VGEE65 pKa = 4.71LYY67 pKa = 11.07NEE69 pKa = 5.2GYY71 pKa = 10.46SLQTALSVTLTVSS84 pKa = 3.14

Molecular weight: 9.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4T221|M4T221_9CAUD Uncharacterized protein OS=Pseudomonas phage UFV-P2 OX=1235661 PE=4 SV=1
MM1 pKa = 7.38RR2 pKa = 11.84KK3 pKa = 9.57ARR5 pKa = 11.84VFYY8 pKa = 10.12EE9 pKa = 4.41IIDD12 pKa = 3.78PTGDD16 pKa = 3.36LTLSGARR23 pKa = 11.84YY24 pKa = 9.02KK25 pKa = 10.53ARR27 pKa = 11.84KK28 pKa = 8.43VMRR31 pKa = 11.84PKK33 pKa = 10.38SRR35 pKa = 11.84KK36 pKa = 9.03AALAQSS42 pKa = 3.75

Molecular weight: 4.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

75

0

75

14154

34

1054

188.7

20.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.69 ± 0.647

1.053 ± 0.145

6.111 ± 0.215

6.45 ± 0.328

3.801 ± 0.249

8.351 ± 0.359

1.809 ± 0.213

4.825 ± 0.238

5.991 ± 0.273

8.252 ± 0.259

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.89 ± 0.14

4.797 ± 0.208

4.5 ± 0.263

4.281 ± 0.291

5.193 ± 0.22

5.278 ± 0.266

5.666 ± 0.258

7.199 ± 0.258

1.611 ± 0.154

3.25 ± 0.213
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski