Amino acid dipepetide frequency for Pseudomonas phage UFV-P2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.609AlaAla: 9.609 ± 1.373
0.565AlaCys: 0.565 ± 0.206
4.239AlaAsp: 4.239 ± 0.632
6.642AlaGlu: 6.642 ± 0.74
2.968AlaPhe: 2.968 ± 0.41
9.468AlaGly: 9.468 ± 0.971
1.272AlaHis: 1.272 ± 0.3
4.522AlaIle: 4.522 ± 0.485
5.37AlaLys: 5.37 ± 0.724
6.854AlaLeu: 6.854 ± 0.745
2.332AlaMet: 2.332 ± 0.398
3.392AlaAsn: 3.392 ± 0.448
3.603AlaPro: 3.603 ± 0.666
3.886AlaGln: 3.886 ± 0.689
4.098AlaArg: 4.098 ± 0.536
3.462AlaSer: 3.462 ± 0.558
4.593AlaThr: 4.593 ± 0.531
6.147AlaVal: 6.147 ± 0.718
1.201AlaTrp: 1.201 ± 0.278
2.685AlaTyr: 2.685 ± 0.512
0.0AlaXaa: 0.0 ± 0.0
Cys
0.707CysAla: 0.707 ± 0.212
0.141CysCys: 0.141 ± 0.098
0.848CysAsp: 0.848 ± 0.277
0.707CysGlu: 0.707 ± 0.243
0.212CysPhe: 0.212 ± 0.123
0.989CysGly: 0.989 ± 0.388
0.212CysHis: 0.212 ± 0.113
0.353CysIle: 0.353 ± 0.153
1.06CysLys: 1.06 ± 0.28
0.777CysLeu: 0.777 ± 0.3
0.424CysMet: 0.424 ± 0.133
0.565CysAsn: 0.565 ± 0.194
0.353CysPro: 0.353 ± 0.126
0.212CysGln: 0.212 ± 0.147
0.283CysArg: 0.283 ± 0.135
0.636CysSer: 0.636 ± 0.203
0.636CysThr: 0.636 ± 0.164
1.06CysVal: 1.06 ± 0.314
0.071CysTrp: 0.071 ± 0.079
0.283CysTyr: 0.283 ± 0.14
0.0CysXaa: 0.0 ± 0.0
Asp
4.805AspAla: 4.805 ± 0.659
0.636AspCys: 0.636 ± 0.206
3.038AspAsp: 3.038 ± 0.513
4.805AspGlu: 4.805 ± 0.602
2.12AspPhe: 2.12 ± 0.346
4.946AspGly: 4.946 ± 0.786
1.272AspHis: 1.272 ± 0.326
3.674AspIle: 3.674 ± 0.559
3.321AspLys: 3.321 ± 0.497
4.734AspLeu: 4.734 ± 0.532
1.484AspMet: 1.484 ± 0.277
2.473AspAsn: 2.473 ± 0.341
3.321AspPro: 3.321 ± 0.532
2.685AspGln: 2.685 ± 0.415
2.614AspArg: 2.614 ± 0.401
3.674AspSer: 3.674 ± 0.532
3.815AspThr: 3.815 ± 0.42
4.31AspVal: 4.31 ± 0.564
1.696AspTrp: 1.696 ± 0.278
1.696AspTyr: 1.696 ± 0.319
0.0AspXaa: 0.0 ± 0.0
Glu
6.995GluAla: 6.995 ± 0.902
0.707GluCys: 0.707 ± 0.238
4.169GluAsp: 4.169 ± 0.424
3.957GluGlu: 3.957 ± 0.606
2.685GluPhe: 2.685 ± 0.356
5.37GluGly: 5.37 ± 0.563
1.484GluHis: 1.484 ± 0.335
2.332GluIle: 2.332 ± 0.567
2.897GluLys: 2.897 ± 0.442
6.571GluLeu: 6.571 ± 0.655
1.837GluMet: 1.837 ± 0.424
2.402GluAsn: 2.402 ± 0.401
2.049GluPro: 2.049 ± 0.366
2.756GluGln: 2.756 ± 0.433
5.087GluArg: 5.087 ± 0.536
2.756GluSer: 2.756 ± 0.406
2.544GluThr: 2.544 ± 0.413
5.441GluVal: 5.441 ± 0.658
0.636GluTrp: 0.636 ± 0.213
1.766GluTyr: 1.766 ± 0.347
0.0GluXaa: 0.0 ± 0.0
Phe
2.332PheAla: 2.332 ± 0.456
0.212PheCys: 0.212 ± 0.11
2.402PheAsp: 2.402 ± 0.393
1.908PheGlu: 1.908 ± 0.389
1.766PhePhe: 1.766 ± 0.381
3.462PheGly: 3.462 ± 0.498
0.777PheHis: 0.777 ± 0.203
2.473PheIle: 2.473 ± 0.46
2.473PheLys: 2.473 ± 0.599
3.392PheLeu: 3.392 ± 0.488
0.919PheMet: 0.919 ± 0.315
2.261PheAsn: 2.261 ± 0.407
1.908PhePro: 1.908 ± 0.4
1.554PheGln: 1.554 ± 0.347
1.908PheArg: 1.908 ± 0.411
1.837PheSer: 1.837 ± 0.363
2.12PheThr: 2.12 ± 0.378
2.756PheVal: 2.756 ± 0.427
0.707PheTrp: 0.707 ± 0.232
0.848PheTyr: 0.848 ± 0.275
0.0PheXaa: 0.0 ± 0.0
Gly
8.196GlyAla: 8.196 ± 1.092
1.131GlyCys: 1.131 ± 0.256
4.381GlyAsp: 4.381 ± 0.62
4.31GlyGlu: 4.31 ± 0.437
2.19GlyPhe: 2.19 ± 0.388
6.642GlyGly: 6.642 ± 1.097
1.766GlyHis: 1.766 ± 0.348
3.957GlyIle: 3.957 ± 0.569
5.511GlyLys: 5.511 ± 0.774
6.147GlyLeu: 6.147 ± 0.626
2.402GlyMet: 2.402 ± 0.455
3.957GlyAsn: 3.957 ± 0.544
2.968GlyPro: 2.968 ± 0.443
3.674GlyGln: 3.674 ± 0.467
3.674GlyArg: 3.674 ± 0.512
6.147GlySer: 6.147 ± 0.898
5.794GlyThr: 5.794 ± 0.719
6.43GlyVal: 6.43 ± 0.479
1.554GlyTrp: 1.554 ± 0.34
2.685GlyTyr: 2.685 ± 0.5
0.0GlyXaa: 0.0 ± 0.0
His
1.342HisAla: 1.342 ± 0.299
0.212HisCys: 0.212 ± 0.12
0.777HisAsp: 0.777 ± 0.224
1.342HisGlu: 1.342 ± 0.299
0.495HisPhe: 0.495 ± 0.171
1.272HisGly: 1.272 ± 0.319
0.212HisHis: 0.212 ± 0.128
0.636HisIle: 0.636 ± 0.213
1.272HisLys: 1.272 ± 0.299
2.402HisLeu: 2.402 ± 0.501
0.495HisMet: 0.495 ± 0.169
0.636HisAsn: 0.636 ± 0.224
0.848HisPro: 0.848 ± 0.215
0.707HisGln: 0.707 ± 0.224
1.201HisArg: 1.201 ± 0.239
0.636HisSer: 0.636 ± 0.173
1.06HisThr: 1.06 ± 0.292
0.989HisVal: 0.989 ± 0.255
0.636HisTrp: 0.636 ± 0.253
0.919HisTyr: 0.919 ± 0.245
0.0HisXaa: 0.0 ± 0.0
Ile
4.098IleAla: 4.098 ± 0.603
0.283IleCys: 0.283 ± 0.131
3.392IleAsp: 3.392 ± 0.466
2.826IleGlu: 2.826 ± 0.545
1.413IlePhe: 1.413 ± 0.309
4.734IleGly: 4.734 ± 0.512
1.131IleHis: 1.131 ± 0.317
2.685IleIle: 2.685 ± 0.602
3.25IleLys: 3.25 ± 0.568
3.603IleLeu: 3.603 ± 0.452
1.342IleMet: 1.342 ± 0.255
2.12IleAsn: 2.12 ± 0.44
2.473IlePro: 2.473 ± 0.424
1.837IleGln: 1.837 ± 0.332
2.897IleArg: 2.897 ± 0.419
2.261IleSer: 2.261 ± 0.449
2.614IleThr: 2.614 ± 0.466
3.109IleVal: 3.109 ± 0.404
0.283IleTrp: 0.283 ± 0.144
1.908IleTyr: 1.908 ± 0.36
0.0IleXaa: 0.0 ± 0.0
Lys
5.935LysAla: 5.935 ± 0.861
0.565LysCys: 0.565 ± 0.26
4.239LysAsp: 4.239 ± 0.569
4.027LysGlu: 4.027 ± 0.505
2.473LysPhe: 2.473 ± 0.398
4.098LysGly: 4.098 ± 0.539
0.989LysHis: 0.989 ± 0.254
2.826LysIle: 2.826 ± 0.401
3.109LysLys: 3.109 ± 0.453
5.299LysLeu: 5.299 ± 0.72
1.766LysMet: 1.766 ± 0.344
3.038LysAsn: 3.038 ± 0.544
3.038LysPro: 3.038 ± 0.491
1.625LysGln: 1.625 ± 0.348
2.544LysArg: 2.544 ± 0.409
3.321LysSer: 3.321 ± 0.406
3.109LysThr: 3.109 ± 0.406
4.169LysVal: 4.169 ± 0.597
1.201LysTrp: 1.201 ± 0.286
2.544LysTyr: 2.544 ± 0.369
0.0LysXaa: 0.0 ± 0.0
Leu
7.631LeuAla: 7.631 ± 0.751
1.201LeuCys: 1.201 ± 0.277
5.37LeuAsp: 5.37 ± 0.621
5.158LeuGlu: 5.158 ± 0.692
3.18LeuPhe: 3.18 ± 0.516
6.5LeuGly: 6.5 ± 0.653
0.777LeuHis: 0.777 ± 0.276
3.674LeuIle: 3.674 ± 0.507
5.158LeuLys: 5.158 ± 0.607
6.924LeuLeu: 6.924 ± 0.846
2.261LeuMet: 2.261 ± 0.42
4.805LeuAsn: 4.805 ± 0.502
3.533LeuPro: 3.533 ± 0.506
4.027LeuGln: 4.027 ± 0.611
4.381LeuArg: 4.381 ± 0.57
4.31LeuSer: 4.31 ± 0.736
4.805LeuThr: 4.805 ± 0.527
5.017LeuVal: 5.017 ± 0.64
0.989LeuTrp: 0.989 ± 0.235
2.826LeuTyr: 2.826 ± 0.531
0.0LeuXaa: 0.0 ± 0.0
Met
2.685MetAla: 2.685 ± 0.28
0.495MetCys: 0.495 ± 0.251
0.989MetAsp: 0.989 ± 0.272
1.625MetGlu: 1.625 ± 0.366
1.201MetPhe: 1.201 ± 0.322
2.826MetGly: 2.826 ± 0.564
0.636MetHis: 0.636 ± 0.178
0.919MetIle: 0.919 ± 0.243
1.413MetLys: 1.413 ± 0.254
2.332MetLeu: 2.332 ± 0.369
0.636MetMet: 0.636 ± 0.19
1.272MetAsn: 1.272 ± 0.28
1.272MetPro: 1.272 ± 0.287
1.272MetGln: 1.272 ± 0.308
1.908MetArg: 1.908 ± 0.409
2.19MetSer: 2.19 ± 0.377
1.908MetThr: 1.908 ± 0.325
1.484MetVal: 1.484 ± 0.357
0.565MetTrp: 0.565 ± 0.204
1.272MetTyr: 1.272 ± 0.281
0.0MetXaa: 0.0 ± 0.0
Asn
3.815AsnAla: 3.815 ± 0.554
0.777AsnCys: 0.777 ± 0.215
2.544AsnAsp: 2.544 ± 0.411
2.261AsnGlu: 2.261 ± 0.401
1.625AsnPhe: 1.625 ± 0.419
3.957AsnGly: 3.957 ± 0.575
0.989AsnHis: 0.989 ± 0.301
3.745AsnIle: 3.745 ± 0.416
2.826AsnLys: 2.826 ± 0.495
3.109AsnLeu: 3.109 ± 0.393
1.342AsnMet: 1.342 ± 0.329
2.685AsnAsn: 2.685 ± 0.57
2.614AsnPro: 2.614 ± 0.461
2.12AsnGln: 2.12 ± 0.35
2.685AsnArg: 2.685 ± 0.402
1.696AsnSer: 1.696 ± 0.31
3.25AsnThr: 3.25 ± 0.439
3.109AsnVal: 3.109 ± 0.479
1.272AsnTrp: 1.272 ± 0.323
1.554AsnTyr: 1.554 ± 0.298
0.0AsnXaa: 0.0 ± 0.0
Pro
3.25ProAla: 3.25 ± 0.567
0.424ProCys: 0.424 ± 0.187
3.745ProAsp: 3.745 ± 0.721
4.593ProGlu: 4.593 ± 0.578
2.049ProPhe: 2.049 ± 0.338
3.038ProGly: 3.038 ± 0.41
0.848ProHis: 0.848 ± 0.223
1.625ProIle: 1.625 ± 0.385
3.25ProLys: 3.25 ± 0.499
2.614ProLeu: 2.614 ± 0.424
1.06ProMet: 1.06 ± 0.241
2.12ProAsn: 2.12 ± 0.426
1.272ProPro: 1.272 ± 0.481
1.837ProGln: 1.837 ± 0.358
1.484ProArg: 1.484 ± 0.351
3.109ProSer: 3.109 ± 0.554
2.685ProThr: 2.685 ± 0.517
3.603ProVal: 3.603 ± 0.471
0.565ProTrp: 0.565 ± 0.192
1.837ProTyr: 1.837 ± 0.397
0.0ProXaa: 0.0 ± 0.0
Gln
3.462GlnAla: 3.462 ± 0.474
0.495GlnCys: 0.495 ± 0.152
2.614GlnAsp: 2.614 ± 0.36
3.18GlnGlu: 3.18 ± 0.409
1.484GlnPhe: 1.484 ± 0.296
3.109GlnGly: 3.109 ± 0.617
0.424GlnHis: 0.424 ± 0.267
1.908GlnIle: 1.908 ± 0.353
1.766GlnLys: 1.766 ± 0.392
5.087GlnLeu: 5.087 ± 0.486
1.554GlnMet: 1.554 ± 0.393
2.12GlnAsn: 2.12 ± 0.427
1.696GlnPro: 1.696 ± 0.367
2.685GlnGln: 2.685 ± 0.747
2.049GlnArg: 2.049 ± 0.426
2.614GlnSer: 2.614 ± 0.535
2.473GlnThr: 2.473 ± 0.422
2.544GlnVal: 2.544 ± 0.403
0.141GlnTrp: 0.141 ± 0.1
1.413GlnTyr: 1.413 ± 0.32
0.0GlnXaa: 0.0 ± 0.0
Arg
3.603ArgAla: 3.603 ± 0.505
0.283ArgCys: 0.283 ± 0.126
3.462ArgAsp: 3.462 ± 0.589
3.321ArgGlu: 3.321 ± 0.587
2.19ArgPhe: 2.19 ± 0.417
3.745ArgGly: 3.745 ± 0.591
0.989ArgHis: 0.989 ± 0.275
2.614ArgIle: 2.614 ± 0.446
3.815ArgLys: 3.815 ± 0.631
3.603ArgLeu: 3.603 ± 0.437
2.402ArgMet: 2.402 ± 0.398
2.473ArgAsn: 2.473 ± 0.416
2.261ArgPro: 2.261 ± 0.396
2.897ArgGln: 2.897 ± 0.435
2.968ArgArg: 2.968 ± 0.39
2.402ArgSer: 2.402 ± 0.382
2.544ArgThr: 2.544 ± 0.422
3.957ArgVal: 3.957 ± 0.504
0.777ArgTrp: 0.777 ± 0.207
1.625ArgTyr: 1.625 ± 0.326
0.0ArgXaa: 0.0 ± 0.0
Ser
3.462SerAla: 3.462 ± 0.733
0.565SerCys: 0.565 ± 0.266
3.109SerAsp: 3.109 ± 0.47
3.109SerGlu: 3.109 ± 0.368
1.837SerPhe: 1.837 ± 0.378
4.451SerGly: 4.451 ± 0.593
1.413SerHis: 1.413 ± 0.272
2.826SerIle: 2.826 ± 0.468
3.038SerLys: 3.038 ± 0.426
4.805SerLeu: 4.805 ± 0.611
1.06SerMet: 1.06 ± 0.223
2.614SerAsn: 2.614 ± 0.455
2.261SerPro: 2.261 ± 0.492
2.12SerGln: 2.12 ± 0.392
2.897SerArg: 2.897 ± 0.39
3.957SerSer: 3.957 ± 0.624
2.402SerThr: 2.402 ± 0.437
4.169SerVal: 4.169 ± 0.465
1.06SerTrp: 1.06 ± 0.333
1.625SerTyr: 1.625 ± 0.349
0.0SerXaa: 0.0 ± 0.0
Thr
5.582ThrAla: 5.582 ± 0.734
0.636ThrCys: 0.636 ± 0.165
3.109ThrAsp: 3.109 ± 0.47
4.522ThrGlu: 4.522 ± 0.494
2.897ThrPhe: 2.897 ± 0.349
5.935ThrGly: 5.935 ± 0.802
0.919ThrHis: 0.919 ± 0.202
2.402ThrIle: 2.402 ± 0.37
2.261ThrLys: 2.261 ± 0.355
4.946ThrLeu: 4.946 ± 0.653
2.12ThrMet: 2.12 ± 0.383
1.837ThrAsn: 1.837 ± 0.378
3.18ThrPro: 3.18 ± 0.41
2.402ThrGln: 2.402 ± 0.352
2.544ThrArg: 2.544 ± 0.439
2.544ThrSer: 2.544 ± 0.37
2.544ThrThr: 2.544 ± 0.405
4.098ThrVal: 4.098 ± 0.441
0.919ThrTrp: 0.919 ± 0.246
1.272ThrTyr: 1.272 ± 0.3
0.0ThrXaa: 0.0 ± 0.0
Val
5.37ValAla: 5.37 ± 0.787
0.636ValCys: 0.636 ± 0.189
5.087ValAsp: 5.087 ± 0.66
4.169ValGlu: 4.169 ± 0.513
2.968ValPhe: 2.968 ± 0.531
5.441ValGly: 5.441 ± 0.688
1.201ValHis: 1.201 ± 0.296
2.826ValIle: 2.826 ± 0.43
4.451ValLys: 4.451 ± 0.642
4.593ValLeu: 4.593 ± 0.826
2.402ValMet: 2.402 ± 0.363
4.31ValAsn: 4.31 ± 0.482
4.098ValPro: 4.098 ± 0.548
2.897ValGln: 2.897 ± 0.478
3.745ValArg: 3.745 ± 0.544
2.826ValSer: 2.826 ± 0.442
5.017ValThr: 5.017 ± 0.628
6.5ValVal: 6.5 ± 0.822
1.201ValTrp: 1.201 ± 0.292
2.261ValTyr: 2.261 ± 0.466
0.0ValXaa: 0.0 ± 0.0
Trp
1.696TrpAla: 1.696 ± 0.361
0.071TrpCys: 0.071 ± 0.063
1.484TrpAsp: 1.484 ± 0.301
0.636TrpGlu: 0.636 ± 0.218
0.707TrpPhe: 0.707 ± 0.21
0.989TrpGly: 0.989 ± 0.327
0.141TrpHis: 0.141 ± 0.09
0.495TrpIle: 0.495 ± 0.166
1.484TrpLys: 1.484 ± 0.292
1.696TrpLeu: 1.696 ± 0.359
0.283TrpMet: 0.283 ± 0.119
0.636TrpAsn: 0.636 ± 0.194
0.424TrpPro: 0.424 ± 0.208
0.424TrpGln: 0.424 ± 0.139
1.413TrpArg: 1.413 ± 0.288
1.06TrpSer: 1.06 ± 0.354
0.707TrpThr: 0.707 ± 0.201
0.989TrpVal: 0.989 ± 0.228
0.212TrpTrp: 0.212 ± 0.12
0.565TrpTyr: 0.565 ± 0.198
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.332TyrAla: 2.332 ± 0.348
0.495TyrCys: 0.495 ± 0.188
2.12TyrAsp: 2.12 ± 0.449
1.413TyrGlu: 1.413 ± 0.281
1.554TyrPhe: 1.554 ± 0.301
2.544TyrGly: 2.544 ± 0.314
0.636TyrHis: 0.636 ± 0.223
1.766TyrIle: 1.766 ± 0.376
2.19TyrLys: 2.19 ± 0.4
3.038TyrLeu: 3.038 ± 0.455
0.707TyrMet: 0.707 ± 0.204
2.261TyrAsn: 2.261 ± 0.324
1.837TyrPro: 1.837 ± 0.386
1.201TyrGln: 1.201 ± 0.273
1.554TyrArg: 1.554 ± 0.332
1.342TyrSer: 1.342 ± 0.271
2.049TyrThr: 2.049 ± 0.301
2.12TyrVal: 2.12 ± 0.414
0.424TyrTrp: 0.424 ± 0.155
0.919TyrTyr: 0.919 ± 0.295
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 75 proteins (14154 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski