Amino acid dipepetide frequency for Rahnella aquatilis (strain ATCC 33071 / DSM 4594 / JCM 1683 / NBRC 105701 / NCIMB 13365 / CIP 78.65)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.309AlaAla: 10.309 ± 0.124
1.082AlaCys: 1.082 ± 0.028
5.189AlaAsp: 5.189 ± 0.065
5.71AlaGlu: 5.71 ± 0.064
3.594AlaPhe: 3.594 ± 0.048
7.977AlaGly: 7.977 ± 0.111
1.793AlaHis: 1.793 ± 0.031
5.588AlaIle: 5.588 ± 0.064
4.0AlaLys: 4.0 ± 0.059
11.371AlaLeu: 11.371 ± 0.101
2.918AlaMet: 2.918 ± 0.043
2.878AlaAsn: 2.878 ± 0.05
3.64AlaPro: 3.64 ± 0.052
4.423AlaGln: 4.423 ± 0.061
5.082AlaArg: 5.082 ± 0.064
5.721AlaSer: 5.721 ± 0.062
4.847AlaThr: 4.847 ± 0.07
6.867AlaVal: 6.867 ± 0.07
1.311AlaTrp: 1.311 ± 0.034
2.313AlaTyr: 2.313 ± 0.037
0.0AlaXaa: 0.0 ± 0.0
Cys
0.926CysAla: 0.926 ± 0.025
0.169CysCys: 0.169 ± 0.01
0.566CysAsp: 0.566 ± 0.02
0.548CysGlu: 0.548 ± 0.021
0.439CysPhe: 0.439 ± 0.018
1.014CysGly: 1.014 ± 0.028
0.314CysHis: 0.314 ± 0.014
0.549CysIle: 0.549 ± 0.019
0.325CysLys: 0.325 ± 0.014
1.02CysLeu: 1.02 ± 0.028
0.23CysMet: 0.23 ± 0.014
0.318CysAsn: 0.318 ± 0.015
0.476CysPro: 0.476 ± 0.02
0.464CysGln: 0.464 ± 0.019
0.579CysArg: 0.579 ± 0.021
0.658CysSer: 0.658 ± 0.019
0.513CysThr: 0.513 ± 0.019
0.734CysVal: 0.734 ± 0.019
0.164CysTrp: 0.164 ± 0.011
0.315CysTyr: 0.315 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
5.185AspAla: 5.185 ± 0.064
0.517AspCys: 0.517 ± 0.019
2.859AspAsp: 2.859 ± 0.046
3.285AspGlu: 3.285 ± 0.044
2.242AspPhe: 2.242 ± 0.036
3.846AspGly: 3.846 ± 0.072
1.04AspHis: 1.04 ± 0.027
3.55AspIle: 3.55 ± 0.05
2.523AspLys: 2.523 ± 0.046
4.852AspLeu: 4.852 ± 0.061
1.407AspMet: 1.407 ± 0.032
2.235AspAsn: 2.235 ± 0.041
2.369AspPro: 2.369 ± 0.04
1.827AspGln: 1.827 ± 0.036
2.561AspArg: 2.561 ± 0.04
2.961AspSer: 2.961 ± 0.044
2.632AspThr: 2.632 ± 0.043
3.953AspVal: 3.953 ± 0.053
0.889AspTrp: 0.889 ± 0.025
1.865AspTyr: 1.865 ± 0.039
0.0AspXaa: 0.0 ± 0.0
Glu
4.865GluAla: 4.865 ± 0.061
0.458GluCys: 0.458 ± 0.02
2.441GluAsp: 2.441 ± 0.038
3.108GluGlu: 3.108 ± 0.054
1.965GluPhe: 1.965 ± 0.037
3.525GluGly: 3.525 ± 0.053
1.387GluHis: 1.387 ± 0.032
3.343GluIle: 3.343 ± 0.049
3.184GluLys: 3.184 ± 0.048
5.663GluLeu: 5.663 ± 0.074
1.72GluMet: 1.72 ± 0.034
2.512GluAsn: 2.512 ± 0.04
2.181GluPro: 2.181 ± 0.045
3.16GluGln: 3.16 ± 0.052
3.347GluArg: 3.347 ± 0.046
3.005GluSer: 3.005 ± 0.041
2.891GluThr: 2.891 ± 0.043
3.491GluVal: 3.491 ± 0.057
0.721GluTrp: 0.721 ± 0.021
1.433GluTyr: 1.433 ± 0.034
0.0GluXaa: 0.0 ± 0.0
Phe
3.646PheAla: 3.646 ± 0.049
0.534PheCys: 0.534 ± 0.017
2.409PheAsp: 2.409 ± 0.043
1.839PheGlu: 1.839 ± 0.039
1.694PhePhe: 1.694 ± 0.041
3.243PheGly: 3.243 ± 0.047
0.832PheHis: 0.832 ± 0.022
2.617PheIle: 2.617 ± 0.044
1.324PheLys: 1.324 ± 0.029
3.412PheLeu: 3.412 ± 0.057
1.023PheMet: 1.023 ± 0.024
1.752PheAsn: 1.752 ± 0.032
1.633PhePro: 1.633 ± 0.037
1.145PheGln: 1.145 ± 0.027
1.931PheArg: 1.931 ± 0.035
3.364PheSer: 3.364 ± 0.051
2.442PheThr: 2.442 ± 0.038
2.514PheVal: 2.514 ± 0.04
0.59PheTrp: 0.59 ± 0.02
1.332PheTyr: 1.332 ± 0.032
0.0PheXaa: 0.0 ± 0.0
Gly
6.221GlyAla: 6.221 ± 0.076
0.958GlyCys: 0.958 ± 0.028
3.763GlyAsp: 3.763 ± 0.07
4.367GlyGlu: 4.367 ± 0.06
3.376GlyPhe: 3.376 ± 0.049
5.598GlyGly: 5.598 ± 0.088
1.677GlyHis: 1.677 ± 0.035
5.069GlyIle: 5.069 ± 0.056
4.156GlyLys: 4.156 ± 0.054
7.69GlyLeu: 7.69 ± 0.071
2.339GlyMet: 2.339 ± 0.037
2.931GlyAsn: 2.931 ± 0.07
2.077GlyPro: 2.077 ± 0.036
3.028GlyGln: 3.028 ± 0.059
3.662GlyArg: 3.662 ± 0.051
4.46GlySer: 4.46 ± 0.068
3.962GlyThr: 3.962 ± 0.079
5.669GlyVal: 5.669 ± 0.066
1.292GlyTrp: 1.292 ± 0.03
2.618GlyTyr: 2.618 ± 0.053
0.0GlyXaa: 0.0 ± 0.0
His
1.876HisAla: 1.876 ± 0.038
0.332HisCys: 0.332 ± 0.013
1.193HisAsp: 1.193 ± 0.029
1.088HisGlu: 1.088 ± 0.022
1.06HisPhe: 1.06 ± 0.025
1.668HisGly: 1.668 ± 0.038
0.73HisHis: 0.73 ± 0.024
1.359HisIle: 1.359 ± 0.028
0.778HisLys: 0.778 ± 0.025
2.256HisLeu: 2.256 ± 0.045
0.581HisMet: 0.581 ± 0.021
0.833HisAsn: 0.833 ± 0.028
1.321HisPro: 1.321 ± 0.032
1.178HisGln: 1.178 ± 0.029
1.201HisArg: 1.201 ± 0.029
1.279HisSer: 1.279 ± 0.029
1.078HisThr: 1.078 ± 0.028
1.286HisVal: 1.286 ± 0.029
0.443HisTrp: 0.443 ± 0.017
0.846HisTyr: 0.846 ± 0.025
0.0HisXaa: 0.0 ± 0.0
Ile
6.26IleAla: 6.26 ± 0.073
0.642IleCys: 0.642 ± 0.021
3.43IleAsp: 3.43 ± 0.054
3.246IleGlu: 3.246 ± 0.048
2.174IlePhe: 2.174 ± 0.038
4.598IleGly: 4.598 ± 0.058
1.216IleHis: 1.216 ± 0.026
3.361IleIle: 3.361 ± 0.053
2.323IleLys: 2.323 ± 0.041
5.235IleLeu: 5.235 ± 0.064
1.308IleMet: 1.308 ± 0.032
2.606IleAsn: 2.606 ± 0.051
2.805IlePro: 2.805 ± 0.043
1.806IleGln: 1.806 ± 0.035
3.025IleArg: 3.025 ± 0.045
4.295IleSer: 4.295 ± 0.068
3.714IleThr: 3.714 ± 0.049
3.781IleVal: 3.781 ± 0.045
0.652IleTrp: 0.652 ± 0.021
1.6IleTyr: 1.6 ± 0.032
0.0IleXaa: 0.0 ± 0.0
Lys
4.121LysAla: 4.121 ± 0.06
0.257LysCys: 0.257 ± 0.012
2.203LysAsp: 2.203 ± 0.042
2.351LysGlu: 2.351 ± 0.044
1.282LysPhe: 1.282 ± 0.025
2.947LysGly: 2.947 ± 0.052
0.896LysHis: 0.896 ± 0.024
2.642LysIle: 2.642 ± 0.042
2.341LysLys: 2.341 ± 0.047
4.342LysLeu: 4.342 ± 0.06
1.3LysMet: 1.3 ± 0.029
1.901LysAsn: 1.901 ± 0.036
2.248LysPro: 2.248 ± 0.044
1.994LysGln: 1.994 ± 0.045
2.339LysArg: 2.339 ± 0.039
2.486LysSer: 2.486 ± 0.043
2.675LysThr: 2.675 ± 0.041
2.963LysVal: 2.963 ± 0.049
0.478LysTrp: 0.478 ± 0.018
1.142LysTyr: 1.142 ± 0.031
0.0LysXaa: 0.0 ± 0.0
Leu
11.108LeuAla: 11.108 ± 0.096
1.25LeuCys: 1.25 ± 0.031
5.466LeuAsp: 5.466 ± 0.067
5.25LeuGlu: 5.25 ± 0.066
4.209LeuPhe: 4.209 ± 0.062
7.393LeuGly: 7.393 ± 0.081
2.272LeuHis: 2.272 ± 0.045
5.961LeuIle: 5.961 ± 0.069
4.606LeuLys: 4.606 ± 0.055
11.905LeuLeu: 11.905 ± 0.136
3.056LeuMet: 3.056 ± 0.054
4.338LeuAsn: 4.338 ± 0.067
5.702LeuPro: 5.702 ± 0.072
4.281LeuGln: 4.281 ± 0.052
5.959LeuArg: 5.959 ± 0.081
7.86LeuSer: 7.86 ± 0.069
6.695LeuThr: 6.695 ± 0.078
6.896LeuVal: 6.896 ± 0.071
1.308LeuTrp: 1.308 ± 0.033
2.609LeuTyr: 2.609 ± 0.038
0.0LeuXaa: 0.0 ± 0.0
Met
2.918MetAla: 2.918 ± 0.048
0.188MetCys: 0.188 ± 0.012
1.183MetAsp: 1.183 ± 0.029
1.224MetGlu: 1.224 ± 0.03
0.937MetPhe: 0.937 ± 0.026
1.84MetGly: 1.84 ± 0.039
0.55MetHis: 0.55 ± 0.019
1.546MetIle: 1.546 ± 0.032
1.414MetLys: 1.414 ± 0.031
3.088MetLeu: 3.088 ± 0.051
0.893MetMet: 0.893 ± 0.021
1.134MetAsn: 1.134 ± 0.026
1.421MetPro: 1.421 ± 0.028
1.244MetGln: 1.244 ± 0.03
1.427MetArg: 1.427 ± 0.032
2.03MetSer: 2.03 ± 0.041
1.856MetThr: 1.856 ± 0.033
1.846MetVal: 1.846 ± 0.032
0.252MetTrp: 0.252 ± 0.012
0.528MetTyr: 0.528 ± 0.022
0.0MetXaa: 0.0 ± 0.0
Asn
3.653AsnAla: 3.653 ± 0.059
0.336AsnCys: 0.336 ± 0.014
2.1AsnAsp: 2.1 ± 0.042
1.815AsnGlu: 1.815 ± 0.032
1.366AsnPhe: 1.366 ± 0.029
3.035AsnGly: 3.035 ± 0.053
0.833AsnHis: 0.833 ± 0.022
2.476AsnIle: 2.476 ± 0.049
1.59AsnLys: 1.59 ± 0.038
3.627AsnLeu: 3.627 ± 0.047
0.947AsnMet: 0.947 ± 0.026
1.707AsnAsn: 1.707 ± 0.048
2.118AsnPro: 2.118 ± 0.036
1.669AsnGln: 1.669 ± 0.036
1.95AsnArg: 1.95 ± 0.039
2.176AsnSer: 2.176 ± 0.044
2.119AsnThr: 2.119 ± 0.049
2.591AsnVal: 2.591 ± 0.046
0.564AsnTrp: 0.564 ± 0.016
1.18AsnTyr: 1.18 ± 0.026
0.0AsnXaa: 0.0 ± 0.0
Pro
4.964ProAla: 4.964 ± 0.075
0.346ProCys: 0.346 ± 0.014
3.008ProAsp: 3.008 ± 0.051
3.377ProGlu: 3.377 ± 0.049
1.839ProPhe: 1.839 ± 0.037
3.437ProGly: 3.437 ± 0.05
1.073ProHis: 1.073 ± 0.028
1.706ProIle: 1.706 ± 0.035
1.629ProLys: 1.629 ± 0.033
5.179ProLeu: 5.179 ± 0.057
1.092ProMet: 1.092 ± 0.027
1.245ProAsn: 1.245 ± 0.027
1.71ProPro: 1.71 ± 0.04
2.397ProGln: 2.397 ± 0.043
1.901ProArg: 1.901 ± 0.036
2.289ProSer: 2.289 ± 0.042
2.032ProThr: 2.032 ± 0.04
4.312ProVal: 4.312 ± 0.056
0.704ProTrp: 0.704 ± 0.025
1.349ProTyr: 1.349 ± 0.033
0.0ProXaa: 0.0 ± 0.0
Gln
4.622GlnAla: 4.622 ± 0.067
0.389GlnCys: 0.389 ± 0.016
2.016GlnAsp: 2.016 ± 0.038
2.109GlnGlu: 2.109 ± 0.042
1.51GlnPhe: 1.51 ± 0.029
3.17GlnGly: 3.17 ± 0.049
1.161GlnHis: 1.161 ± 0.026
2.43GlnIle: 2.43 ± 0.042
1.892GlnLys: 1.892 ± 0.032
4.926GlnLeu: 4.926 ± 0.058
1.168GlnMet: 1.168 ± 0.024
1.632GlnAsn: 1.632 ± 0.035
2.285GlnPro: 2.285 ± 0.037
3.152GlnGln: 3.152 ± 0.07
2.884GlnArg: 2.884 ± 0.049
2.487GlnSer: 2.487 ± 0.04
2.309GlnThr: 2.309 ± 0.05
3.091GlnVal: 3.091 ± 0.047
0.686GlnTrp: 0.686 ± 0.021
1.177GlnTyr: 1.177 ± 0.03
0.0GlnXaa: 0.0 ± 0.0
Arg
4.378ArgAla: 4.378 ± 0.057
0.54ArgCys: 0.54 ± 0.019
2.84ArgAsp: 2.84 ± 0.046
3.505ArgGlu: 3.505 ± 0.049
2.448ArgPhe: 2.448 ± 0.042
3.2ArgGly: 3.2 ± 0.049
1.481ArgHis: 1.481 ± 0.036
3.333ArgIle: 3.333 ± 0.043
2.325ArgLys: 2.325 ± 0.038
6.149ArgLeu: 6.149 ± 0.085
1.498ArgMet: 1.498 ± 0.037
1.964ArgAsn: 1.964 ± 0.033
2.155ArgPro: 2.155 ± 0.04
2.943ArgGln: 2.943 ± 0.051
3.252ArgArg: 3.252 ± 0.058
2.784ArgSer: 2.784 ± 0.047
2.557ArgThr: 2.557 ± 0.043
3.694ArgVal: 3.694 ± 0.052
0.877ArgTrp: 0.877 ± 0.026
1.973ArgTyr: 1.973 ± 0.039
0.0ArgXaa: 0.0 ± 0.0
Ser
6.155SerAla: 6.155 ± 0.072
0.56SerCys: 0.56 ± 0.021
3.25SerAsp: 3.25 ± 0.044
3.322SerGlu: 3.322 ± 0.042
2.481SerPhe: 2.481 ± 0.043
5.68SerGly: 5.68 ± 0.074
1.492SerHis: 1.492 ± 0.03
3.073SerIle: 3.073 ± 0.051
2.232SerLys: 2.232 ± 0.046
7.247SerLeu: 7.247 ± 0.084
1.567SerMet: 1.567 ± 0.033
2.046SerAsn: 2.046 ± 0.035
2.896SerPro: 2.896 ± 0.045
2.83SerGln: 2.83 ± 0.045
3.397SerArg: 3.397 ± 0.052
3.864SerSer: 3.864 ± 0.057
3.102SerThr: 3.102 ± 0.055
4.646SerVal: 4.646 ± 0.063
0.939SerTrp: 0.939 ± 0.027
1.79SerTyr: 1.79 ± 0.037
0.0SerXaa: 0.0 ± 0.0
Thr
5.169ThrAla: 5.169 ± 0.077
0.509ThrCys: 0.509 ± 0.018
2.868ThrAsp: 2.868 ± 0.054
2.788ThrGlu: 2.788 ± 0.043
2.076ThrPhe: 2.076 ± 0.034
4.917ThrGly: 4.917 ± 0.08
1.266ThrHis: 1.266 ± 0.028
2.618ThrIle: 2.618 ± 0.043
1.619ThrLys: 1.619 ± 0.036
7.44ThrLeu: 7.44 ± 0.09
1.102ThrMet: 1.102 ± 0.027
1.494ThrAsn: 1.494 ± 0.044
3.339ThrPro: 3.339 ± 0.049
2.395ThrGln: 2.395 ± 0.047
2.976ThrArg: 2.976 ± 0.044
3.184ThrSer: 3.184 ± 0.058
2.976ThrThr: 2.976 ± 0.064
4.04ThrVal: 4.04 ± 0.088
0.702ThrTrp: 0.702 ± 0.026
1.355ThrTyr: 1.355 ± 0.034
0.0ThrXaa: 0.0 ± 0.0
Val
6.87ValAla: 6.87 ± 0.071
0.739ValCys: 0.739 ± 0.024
3.622ValAsp: 3.622 ± 0.048
3.664ValGlu: 3.664 ± 0.048
2.684ValPhe: 2.684 ± 0.05
4.648ValGly: 4.648 ± 0.057
1.214ValHis: 1.214 ± 0.026
4.679ValIle: 4.679 ± 0.057
3.099ValLys: 3.099 ± 0.052
7.359ValLeu: 7.359 ± 0.082
2.299ValMet: 2.299 ± 0.038
2.773ValAsn: 2.773 ± 0.048
3.106ValPro: 3.106 ± 0.047
2.47ValGln: 2.47 ± 0.04
3.642ValArg: 3.642 ± 0.056
5.052ValSer: 5.052 ± 0.055
4.397ValThr: 4.397 ± 0.079
5.382ValVal: 5.382 ± 0.059
0.928ValTrp: 0.928 ± 0.023
1.724ValTyr: 1.724 ± 0.033
0.0ValXaa: 0.0 ± 0.0
Trp
0.967TrpAla: 0.967 ± 0.026
0.15TrpCys: 0.15 ± 0.009
0.645TrpAsp: 0.645 ± 0.02
0.582TrpGlu: 0.582 ± 0.019
0.637TrpPhe: 0.637 ± 0.019
0.94TrpGly: 0.94 ± 0.025
0.423TrpHis: 0.423 ± 0.016
0.726TrpIle: 0.726 ± 0.025
0.563TrpLys: 0.563 ± 0.02
2.111TrpLeu: 2.111 ± 0.043
0.443TrpMet: 0.443 ± 0.018
0.52TrpAsn: 0.52 ± 0.019
0.636TrpPro: 0.636 ± 0.021
1.031TrpGln: 1.031 ± 0.029
0.923TrpArg: 0.923 ± 0.027
0.792TrpSer: 0.792 ± 0.026
0.582TrpThr: 0.582 ± 0.021
0.888TrpVal: 0.888 ± 0.025
0.23TrpTrp: 0.23 ± 0.014
0.382TrpTyr: 0.382 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.398TyrAla: 2.398 ± 0.039
0.364TyrCys: 0.364 ± 0.015
1.548TyrAsp: 1.548 ± 0.039
1.223TyrGlu: 1.223 ± 0.03
1.26TyrPhe: 1.26 ± 0.03
2.235TyrGly: 2.235 ± 0.043
0.737TyrHis: 0.737 ± 0.024
1.449TyrIle: 1.449 ± 0.03
0.986TyrLys: 0.986 ± 0.028
3.249TyrLeu: 3.249 ± 0.046
0.631TyrMet: 0.631 ± 0.02
1.033TyrAsn: 1.033 ± 0.029
1.472TyrPro: 1.472 ± 0.032
1.662TyrGln: 1.662 ± 0.034
1.857TyrArg: 1.857 ± 0.034
1.824TyrSer: 1.824 ± 0.037
1.452TyrThr: 1.452 ± 0.032
1.708TyrVal: 1.708 ± 0.035
0.441TyrTrp: 0.441 ± 0.018
0.979TyrTyr: 0.979 ± 0.026
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4841 proteins (1570583 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski