Staphylococcus phage phiSP119-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fibralongavirus; unclassified Fibralongavirus

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A499SJE6|A0A499SJE6_9CAUD Terminase large subunit OS=Staphylococcus phage phiSP119-1 OX=2491316 PE=4 SV=1
MM1 pKa = 7.64KK2 pKa = 10.61YY3 pKa = 10.24IITTAMILYY12 pKa = 9.66IAYY15 pKa = 9.77DD16 pKa = 3.4YY17 pKa = 10.85YY18 pKa = 10.68IRR20 pKa = 11.84LTANDD25 pKa = 5.29DD26 pKa = 3.16IDD28 pKa = 3.9TFNLQDD34 pKa = 5.43HH35 pKa = 7.16IDD37 pKa = 3.84LNNIRR42 pKa = 11.84AEE44 pKa = 4.23VTDD47 pKa = 3.7

Molecular weight: 5.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A499SSB1|A0A499SSB1_9CAUD BIG2 domain-containing protein OS=Staphylococcus phage phiSP119-1 OX=2491316 PE=4 SV=1
MM1 pKa = 7.57SKK3 pKa = 10.68YY4 pKa = 10.37SDD6 pKa = 3.64YY7 pKa = 10.77IYY9 pKa = 10.71RR10 pKa = 11.84RR11 pKa = 11.84NEE13 pKa = 3.16NRR15 pKa = 11.84KK16 pKa = 9.14FYY18 pKa = 10.44MRR20 pKa = 11.84AKK22 pKa = 7.51WRR24 pKa = 11.84KK25 pKa = 8.17VRR27 pKa = 11.84HH28 pKa = 4.38QVLVRR33 pKa = 11.84DD34 pKa = 4.08HH35 pKa = 7.11FEE37 pKa = 4.18CLHH40 pKa = 6.06CKK42 pKa = 10.36KK43 pKa = 10.31EE44 pKa = 4.18GKK46 pKa = 8.33LTINQQQSLEE56 pKa = 4.02VDD58 pKa = 4.02HH59 pKa = 8.2ILEE62 pKa = 4.26LSEE65 pKa = 5.38RR66 pKa = 11.84PDD68 pKa = 3.86LAYY71 pKa = 10.57DD72 pKa = 4.14LNNLQTLCKK81 pKa = 8.44YY82 pKa = 9.98HH83 pKa = 6.74HH84 pKa = 6.48NKK86 pKa = 8.89KK87 pKa = 9.94HH88 pKa = 6.1GRR90 pKa = 11.84FEE92 pKa = 4.64HH93 pKa = 5.99NKK95 pKa = 9.75KK96 pKa = 10.21SGKK99 pKa = 9.53NKK101 pKa = 10.13YY102 pKa = 8.81QDD104 pKa = 3.56EE105 pKa = 4.44RR106 pKa = 11.84WW107 pKa = 3.36

Molecular weight: 13.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

15073

41

2031

239.3

27.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.017 ± 0.51

0.425 ± 0.097

6.76 ± 0.562

7.231 ± 0.478

4.16 ± 0.202

6.077 ± 0.499

1.798 ± 0.187

6.455 ± 0.32

8.923 ± 0.358

7.775 ± 0.385

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.528 ± 0.234

6.661 ± 0.315

2.886 ± 0.326

4.365 ± 0.272

4.087 ± 0.264

6.051 ± 0.361

6.369 ± 0.463

6.097 ± 0.213

1.168 ± 0.113

4.166 ± 0.343
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski