Amino acid dipepetide frequency for Staphylococcus phage phiSP119-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.118AlaAla: 3.118 ± 1.079
0.066AlaCys: 0.066 ± 0.072
4.114AlaAsp: 4.114 ± 0.686
3.782AlaGlu: 3.782 ± 0.537
2.986AlaPhe: 2.986 ± 0.353
3.45AlaGly: 3.45 ± 0.586
0.796AlaHis: 0.796 ± 0.272
4.246AlaIle: 4.246 ± 0.477
4.18AlaLys: 4.18 ± 0.634
6.303AlaLeu: 6.303 ± 0.838
1.725AlaMet: 1.725 ± 0.525
4.047AlaAsn: 4.047 ± 0.554
1.261AlaPro: 1.261 ± 0.263
2.853AlaGln: 2.853 ± 0.695
2.654AlaArg: 2.654 ± 0.465
4.578AlaSer: 4.578 ± 0.508
3.118AlaThr: 3.118 ± 0.748
3.118AlaVal: 3.118 ± 0.58
0.796AlaTrp: 0.796 ± 0.209
2.986AlaTyr: 2.986 ± 0.512
0.0AlaXaa: 0.0 ± 0.0
Cys
0.199CysAla: 0.199 ± 0.116
0.0CysCys: 0.0 ± 0.0
0.066CysAsp: 0.066 ± 0.068
0.133CysGlu: 0.133 ± 0.107
0.199CysPhe: 0.199 ± 0.09
0.332CysGly: 0.332 ± 0.192
0.133CysHis: 0.133 ± 0.099
0.531CysIle: 0.531 ± 0.183
0.398CysLys: 0.398 ± 0.182
0.531CysLeu: 0.531 ± 0.223
0.066CysMet: 0.066 ± 0.071
0.133CysAsn: 0.133 ± 0.097
0.133CysPro: 0.133 ± 0.102
0.133CysGln: 0.133 ± 0.101
0.332CysArg: 0.332 ± 0.169
0.199CysSer: 0.199 ± 0.133
0.133CysThr: 0.133 ± 0.088
0.398CysVal: 0.398 ± 0.141
0.133CysTrp: 0.133 ± 0.095
0.066CysTyr: 0.066 ± 0.073
0.0CysXaa: 0.0 ± 0.0
Asp
3.782AspAla: 3.782 ± 0.48
0.133AspCys: 0.133 ± 0.098
5.042AspAsp: 5.042 ± 0.813
5.573AspGlu: 5.573 ± 0.702
3.516AspPhe: 3.516 ± 0.631
4.379AspGly: 4.379 ± 0.843
0.796AspHis: 0.796 ± 0.327
4.18AspIle: 4.18 ± 0.601
6.502AspLys: 6.502 ± 0.869
5.441AspLeu: 5.441 ± 0.645
2.322AspMet: 2.322 ± 0.604
3.915AspAsn: 3.915 ± 0.792
1.725AspPro: 1.725 ± 0.276
1.393AspGln: 1.393 ± 0.357
2.455AspArg: 2.455 ± 0.455
3.583AspSer: 3.583 ± 0.87
3.915AspThr: 3.915 ± 0.676
4.512AspVal: 4.512 ± 0.507
0.796AspTrp: 0.796 ± 0.218
3.649AspTyr: 3.649 ± 0.613
0.0AspXaa: 0.0 ± 0.0
Glu
4.843GluAla: 4.843 ± 0.503
0.663GluCys: 0.663 ± 0.268
3.516GluAsp: 3.516 ± 0.526
5.573GluGlu: 5.573 ± 0.96
3.118GluPhe: 3.118 ± 0.523
3.45GluGly: 3.45 ± 0.517
1.261GluHis: 1.261 ± 0.397
4.445GluIle: 4.445 ± 0.597
6.303GluLys: 6.303 ± 0.915
6.635GluLeu: 6.635 ± 0.912
2.322GluMet: 2.322 ± 0.37
5.175GluAsn: 5.175 ± 0.931
2.389GluPro: 2.389 ± 0.827
3.649GluGln: 3.649 ± 0.677
3.384GluArg: 3.384 ± 0.617
3.384GluSer: 3.384 ± 0.468
4.047GluThr: 4.047 ± 0.579
4.313GluVal: 4.313 ± 0.824
0.863GluTrp: 0.863 ± 0.2
2.986GluTyr: 2.986 ± 0.595
0.0GluXaa: 0.0 ± 0.0
Phe
2.322PheAla: 2.322 ± 0.469
0.332PheCys: 0.332 ± 0.146
2.787PheAsp: 2.787 ± 0.553
2.986PheGlu: 2.986 ± 0.67
1.725PhePhe: 1.725 ± 0.33
3.052PheGly: 3.052 ± 0.455
0.199PheHis: 0.199 ± 0.121
2.853PheIle: 2.853 ± 0.449
4.512PheLys: 4.512 ± 0.646
2.389PheLeu: 2.389 ± 0.403
0.863PheMet: 0.863 ± 0.276
3.516PheAsn: 3.516 ± 0.663
0.663PhePro: 0.663 ± 0.18
1.327PheGln: 1.327 ± 0.364
1.393PheArg: 1.393 ± 0.346
3.052PheSer: 3.052 ± 0.401
2.787PheThr: 2.787 ± 0.434
2.455PheVal: 2.455 ± 0.47
0.398PheTrp: 0.398 ± 0.163
1.99PheTyr: 1.99 ± 0.379
0.0PheXaa: 0.0 ± 0.0
Gly
4.18GlyAla: 4.18 ± 1.056
0.265GlyCys: 0.265 ± 0.106
3.715GlyAsp: 3.715 ± 0.718
3.384GlyGlu: 3.384 ± 0.636
2.588GlyPhe: 2.588 ± 0.391
5.242GlyGly: 5.242 ± 1.095
1.393GlyHis: 1.393 ± 0.372
4.512GlyIle: 4.512 ± 0.674
5.573GlyLys: 5.573 ± 0.69
4.976GlyLeu: 4.976 ± 0.861
0.663GlyMet: 0.663 ± 0.273
3.981GlyAsn: 3.981 ± 0.824
1.128GlyPro: 1.128 ± 0.262
2.588GlyGln: 2.588 ± 0.732
2.057GlyArg: 2.057 ± 0.348
2.853GlySer: 2.853 ± 0.528
4.711GlyThr: 4.711 ± 0.697
3.848GlyVal: 3.848 ± 0.659
0.796GlyTrp: 0.796 ± 0.254
2.322GlyTyr: 2.322 ± 0.322
0.0GlyXaa: 0.0 ± 0.0
His
1.327HisAla: 1.327 ± 0.339
0.265HisCys: 0.265 ± 0.141
1.261HisAsp: 1.261 ± 0.323
1.261HisGlu: 1.261 ± 0.283
0.597HisPhe: 0.597 ± 0.241
1.128HisGly: 1.128 ± 0.243
0.531HisHis: 0.531 ± 0.269
1.062HisIle: 1.062 ± 0.282
1.062HisLys: 1.062 ± 0.236
1.46HisLeu: 1.46 ± 0.404
0.332HisMet: 0.332 ± 0.13
1.526HisAsn: 1.526 ± 0.575
0.597HisPro: 0.597 ± 0.184
0.663HisGln: 0.663 ± 0.216
0.531HisArg: 0.531 ± 0.258
1.327HisSer: 1.327 ± 0.276
0.995HisThr: 0.995 ± 0.205
0.73HisVal: 0.73 ± 0.259
0.199HisTrp: 0.199 ± 0.129
1.128HisTyr: 1.128 ± 0.336
0.0HisXaa: 0.0 ± 0.0
Ile
4.379IleAla: 4.379 ± 0.76
0.066IleCys: 0.066 ± 0.057
5.573IleAsp: 5.573 ± 0.778
4.711IleGlu: 4.711 ± 0.853
2.389IlePhe: 2.389 ± 0.545
2.72IleGly: 2.72 ± 0.391
0.995IleHis: 0.995 ± 0.239
4.777IleIle: 4.777 ± 0.696
6.635IleLys: 6.635 ± 0.872
4.114IleLeu: 4.114 ± 0.478
1.194IleMet: 1.194 ± 0.285
4.976IleAsn: 4.976 ± 0.652
1.592IlePro: 1.592 ± 0.327
2.322IleGln: 2.322 ± 0.392
2.986IleArg: 2.986 ± 0.529
4.445IleSer: 4.445 ± 0.433
3.715IleThr: 3.715 ± 0.48
4.114IleVal: 4.114 ± 0.713
0.464IleTrp: 0.464 ± 0.152
2.389IleTyr: 2.389 ± 0.397
0.0IleXaa: 0.0 ± 0.0
Lys
5.441LysAla: 5.441 ± 0.622
0.133LysCys: 0.133 ± 0.091
7.099LysAsp: 7.099 ± 0.762
6.9LysGlu: 6.9 ± 1.018
3.052LysPhe: 3.052 ± 0.521
5.573LysGly: 5.573 ± 0.929
1.791LysHis: 1.791 ± 0.437
5.441LysIle: 5.441 ± 0.534
7.431LysLys: 7.431 ± 1.024
6.369LysLeu: 6.369 ± 0.728
2.588LysMet: 2.588 ± 0.478
4.976LysAsn: 4.976 ± 0.648
2.322LysPro: 2.322 ± 0.376
5.573LysGln: 5.573 ± 0.569
4.047LysArg: 4.047 ± 0.589
3.848LysSer: 3.848 ± 0.772
5.109LysThr: 5.109 ± 0.644
6.701LysVal: 6.701 ± 0.818
1.46LysTrp: 1.46 ± 0.468
3.384LysTyr: 3.384 ± 0.687
0.0LysXaa: 0.0 ± 0.0
Leu
3.981LeuAla: 3.981 ± 0.657
0.332LeuCys: 0.332 ± 0.163
5.64LeuAsp: 5.64 ± 0.925
5.839LeuGlu: 5.839 ± 0.8
3.185LeuPhe: 3.185 ± 0.401
3.715LeuGly: 3.715 ± 0.547
1.393LeuHis: 1.393 ± 0.352
4.445LeuIle: 4.445 ± 0.532
8.493LeuLys: 8.493 ± 0.791
5.839LeuLeu: 5.839 ± 0.762
2.256LeuMet: 2.256 ± 0.477
5.64LeuAsn: 5.64 ± 0.54
1.725LeuPro: 1.725 ± 0.314
3.649LeuGln: 3.649 ± 0.661
3.715LeuArg: 3.715 ± 0.471
5.308LeuSer: 5.308 ± 0.86
5.374LeuThr: 5.374 ± 0.484
3.583LeuVal: 3.583 ± 0.422
0.73LeuTrp: 0.73 ± 0.208
2.919LeuTyr: 2.919 ± 0.568
0.0LeuXaa: 0.0 ± 0.0
Met
1.261MetAla: 1.261 ± 0.242
0.066MetCys: 0.066 ± 0.067
0.796MetAsp: 0.796 ± 0.234
1.924MetGlu: 1.924 ± 0.372
1.327MetPhe: 1.327 ± 0.345
1.194MetGly: 1.194 ± 0.357
0.531MetHis: 0.531 ± 0.19
1.592MetIle: 1.592 ± 0.381
2.322MetLys: 2.322 ± 0.471
1.924MetLeu: 1.924 ± 0.37
0.597MetMet: 0.597 ± 0.217
1.327MetAsn: 1.327 ± 0.285
1.194MetPro: 1.194 ± 0.221
1.526MetGln: 1.526 ± 0.373
1.393MetArg: 1.393 ± 0.325
2.189MetSer: 2.189 ± 0.802
1.924MetThr: 1.924 ± 0.312
0.863MetVal: 0.863 ± 0.193
0.398MetTrp: 0.398 ± 0.143
0.929MetTyr: 0.929 ± 0.194
0.0MetXaa: 0.0 ± 0.0
Asn
3.848AsnAla: 3.848 ± 0.578
0.199AsnCys: 0.199 ± 0.112
4.246AsnAsp: 4.246 ± 0.64
4.976AsnGlu: 4.976 ± 0.823
2.322AsnPhe: 2.322 ± 0.444
5.441AsnGly: 5.441 ± 0.738
0.796AsnHis: 0.796 ± 0.193
4.18AsnIle: 4.18 ± 0.595
5.374AsnLys: 5.374 ± 0.542
5.374AsnLeu: 5.374 ± 0.547
1.592AsnMet: 1.592 ± 0.371
4.313AsnAsn: 4.313 ± 0.546
2.787AsnPro: 2.787 ± 0.514
3.118AsnGln: 3.118 ± 0.705
2.322AsnArg: 2.322 ± 0.328
3.848AsnSer: 3.848 ± 0.463
4.18AsnThr: 4.18 ± 0.685
4.313AsnVal: 4.313 ± 0.703
0.796AsnTrp: 0.796 ± 0.265
2.588AsnTyr: 2.588 ± 0.535
0.0AsnXaa: 0.0 ± 0.0
Pro
1.062ProAla: 1.062 ± 0.308
0.133ProCys: 0.133 ± 0.101
1.46ProAsp: 1.46 ± 0.325
2.986ProGlu: 2.986 ± 1.071
1.194ProPhe: 1.194 ± 0.333
1.791ProGly: 1.791 ± 0.57
0.73ProHis: 0.73 ± 0.233
1.194ProIle: 1.194 ± 0.278
2.455ProLys: 2.455 ± 0.353
2.588ProLeu: 2.588 ± 0.54
0.398ProMet: 0.398 ± 0.159
1.659ProAsn: 1.659 ± 0.289
0.929ProPro: 0.929 ± 0.198
1.46ProGln: 1.46 ± 0.453
1.194ProArg: 1.194 ± 0.282
2.919ProSer: 2.919 ± 0.913
1.924ProThr: 1.924 ± 0.873
1.526ProVal: 1.526 ± 0.36
0.265ProTrp: 0.265 ± 0.171
0.995ProTyr: 0.995 ± 0.222
0.0ProXaa: 0.0 ± 0.0
Gln
2.986GlnAla: 2.986 ± 0.473
0.133GlnCys: 0.133 ± 0.1
2.986GlnAsp: 2.986 ± 0.479
2.322GlnGlu: 2.322 ± 0.43
1.592GlnPhe: 1.592 ± 0.292
2.389GlnGly: 2.389 ± 0.566
1.128GlnHis: 1.128 ± 0.479
2.72GlnIle: 2.72 ± 0.442
3.45GlnLys: 3.45 ± 0.63
3.185GlnLeu: 3.185 ± 0.317
0.995GlnMet: 0.995 ± 0.284
3.118GlnAsn: 3.118 ± 0.405
1.924GlnPro: 1.924 ± 0.999
2.322GlnGln: 2.322 ± 0.438
1.99GlnArg: 1.99 ± 0.471
2.72GlnSer: 2.72 ± 0.356
2.521GlnThr: 2.521 ± 0.459
2.322GlnVal: 2.322 ± 0.521
0.464GlnTrp: 0.464 ± 0.164
2.389GlnTyr: 2.389 ± 0.366
0.0GlnXaa: 0.0 ± 0.0
Arg
2.986ArgAla: 2.986 ± 0.566
0.133ArgCys: 0.133 ± 0.092
2.72ArgAsp: 2.72 ± 0.556
3.052ArgGlu: 3.052 ± 0.427
2.256ArgPhe: 2.256 ± 0.383
1.858ArgGly: 1.858 ± 0.411
0.597ArgHis: 0.597 ± 0.168
2.189ArgIle: 2.189 ± 0.396
3.583ArgLys: 3.583 ± 0.518
3.981ArgLeu: 3.981 ± 0.596
0.796ArgMet: 0.796 ± 0.243
2.389ArgAsn: 2.389 ± 0.415
1.062ArgPro: 1.062 ± 0.284
1.858ArgGln: 1.858 ± 0.35
1.393ArgArg: 1.393 ± 0.325
1.924ArgSer: 1.924 ± 0.518
3.118ArgThr: 3.118 ± 0.425
2.919ArgVal: 2.919 ± 0.393
0.332ArgTrp: 0.332 ± 0.167
1.725ArgTyr: 1.725 ± 0.347
0.0ArgXaa: 0.0 ± 0.0
Ser
3.516SerAla: 3.516 ± 0.654
0.199SerCys: 0.199 ± 0.12
4.445SerAsp: 4.445 ± 0.71
4.843SerGlu: 4.843 ± 0.591
2.389SerPhe: 2.389 ± 0.482
4.445SerGly: 4.445 ± 0.932
1.128SerHis: 1.128 ± 0.274
3.715SerIle: 3.715 ± 0.803
5.706SerLys: 5.706 ± 0.534
3.516SerLeu: 3.516 ± 0.449
2.455SerMet: 2.455 ± 0.488
4.91SerAsn: 4.91 ± 0.637
1.659SerPro: 1.659 ± 0.546
2.256SerGln: 2.256 ± 0.365
1.725SerArg: 1.725 ± 0.439
3.516SerSer: 3.516 ± 0.744
3.715SerThr: 3.715 ± 0.562
3.981SerVal: 3.981 ± 0.498
0.73SerTrp: 0.73 ± 0.222
1.592SerTyr: 1.592 ± 0.358
0.0SerXaa: 0.0 ± 0.0
Thr
3.118ThrAla: 3.118 ± 0.598
0.265ThrCys: 0.265 ± 0.138
3.715ThrAsp: 3.715 ± 0.537
4.114ThrGlu: 4.114 ± 0.691
3.317ThrPhe: 3.317 ± 0.537
3.649ThrGly: 3.649 ± 0.694
1.791ThrHis: 1.791 ± 0.352
4.91ThrIle: 4.91 ± 0.72
4.711ThrLys: 4.711 ± 0.55
4.246ThrLeu: 4.246 ± 0.464
1.46ThrMet: 1.46 ± 0.512
3.45ThrAsn: 3.45 ± 0.488
2.787ThrPro: 2.787 ± 0.805
2.986ThrGln: 2.986 ± 0.674
2.521ThrArg: 2.521 ± 0.362
3.782ThrSer: 3.782 ± 0.616
5.042ThrThr: 5.042 ± 0.854
4.644ThrVal: 4.644 ± 0.611
0.796ThrTrp: 0.796 ± 0.207
2.389ThrTyr: 2.389 ± 0.613
0.0ThrXaa: 0.0 ± 0.0
Val
4.578ValAla: 4.578 ± 0.68
0.531ValCys: 0.531 ± 0.219
4.246ValAsp: 4.246 ± 0.526
4.246ValGlu: 4.246 ± 0.786
2.057ValPhe: 2.057 ± 0.391
3.45ValGly: 3.45 ± 0.501
0.929ValHis: 0.929 ± 0.235
3.915ValIle: 3.915 ± 0.541
6.104ValLys: 6.104 ± 0.783
4.512ValLeu: 4.512 ± 0.57
1.194ValMet: 1.194 ± 0.332
4.512ValAsn: 4.512 ± 0.9
1.659ValPro: 1.659 ± 0.358
1.858ValGln: 1.858 ± 0.299
2.787ValArg: 2.787 ± 0.411
3.516ValSer: 3.516 ± 0.366
4.246ValThr: 4.246 ± 0.818
3.583ValVal: 3.583 ± 0.548
0.73ValTrp: 0.73 ± 0.245
2.322ValTyr: 2.322 ± 0.492
0.0ValXaa: 0.0 ± 0.0
Trp
0.73TrpAla: 0.73 ± 0.262
0.066TrpCys: 0.066 ± 0.066
0.995TrpAsp: 0.995 ± 0.244
0.929TrpGlu: 0.929 ± 0.312
0.464TrpPhe: 0.464 ± 0.187
0.398TrpGly: 0.398 ± 0.14
0.133TrpHis: 0.133 ± 0.104
0.929TrpIle: 0.929 ± 0.241
1.062TrpLys: 1.062 ± 0.25
0.796TrpLeu: 0.796 ± 0.187
0.332TrpMet: 0.332 ± 0.162
0.73TrpAsn: 0.73 ± 0.242
0.265TrpPro: 0.265 ± 0.229
0.531TrpGln: 0.531 ± 0.172
0.597TrpArg: 0.597 ± 0.234
0.929TrpSer: 0.929 ± 0.241
0.663TrpThr: 0.663 ± 0.216
0.398TrpVal: 0.398 ± 0.161
0.0TrpTrp: 0.0 ± 0.0
0.73TrpTyr: 0.73 ± 0.234
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.521TyrAla: 2.521 ± 0.398
0.265TyrCys: 0.265 ± 0.146
3.185TyrAsp: 3.185 ± 0.637
2.787TyrGlu: 2.787 ± 0.492
1.327TyrPhe: 1.327 ± 0.345
3.118TyrGly: 3.118 ± 0.484
0.929TyrHis: 0.929 ± 0.362
2.72TyrIle: 2.72 ± 0.554
3.384TyrLys: 3.384 ± 0.545
3.583TyrLeu: 3.583 ± 0.467
1.062TyrMet: 1.062 ± 0.303
2.322TyrAsn: 2.322 ± 0.425
1.062TyrPro: 1.062 ± 0.279
1.592TyrGln: 1.592 ± 0.266
1.393TyrArg: 1.393 ± 0.28
2.588TyrSer: 2.588 ± 0.457
2.455TyrThr: 2.455 ± 0.628
2.654TyrVal: 2.654 ± 0.494
0.531TyrTrp: 0.531 ± 0.171
2.189TyrTyr: 2.189 ± 0.554
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 63 proteins (15073 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski