Planctomycetes bacterium CA13

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; unclassified Planctomycetes

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7247 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C5Z5F5|A0A5C5Z5F5_9BACT Flagellar basal body protein OS=Planctomycetes bacterium CA13 OX=2527986 GN=flgG_2 PE=3 SV=1
MM1 pKa = 7.02NQDD4 pKa = 3.29DD5 pKa = 4.11CLEE8 pKa = 4.29RR9 pKa = 11.84LGLTPPPNSATEE21 pKa = 3.65IRR23 pKa = 11.84RR24 pKa = 11.84ILAAQTDD31 pKa = 4.77LEE33 pKa = 4.77SKK35 pKa = 10.42SQGDD39 pKa = 3.8GDD41 pKa = 3.85QEE43 pKa = 4.61LIKK46 pKa = 9.46LTCVQLFALGDD57 pKa = 3.58IADD60 pKa = 4.86SLVIWNAKK68 pKa = 8.33QCSFDD73 pKa = 3.61MSCSIDD79 pKa = 3.53VQLLCGAGVDD89 pKa = 3.75EE90 pKa = 4.49TLVYY94 pKa = 10.76LDD96 pKa = 5.96DD97 pKa = 5.75LGTDD101 pKa = 3.75PASAAATYY109 pKa = 10.05IRR111 pKa = 11.84EE112 pKa = 4.62CITTGDD118 pKa = 4.21FNDD121 pKa = 4.31FTPPDD126 pKa = 3.24WLADD130 pKa = 3.3YY131 pKa = 10.52RR132 pKa = 11.84RR133 pKa = 11.84YY134 pKa = 10.85YY135 pKa = 10.69GVV137 pKa = 3.05

Molecular weight: 15.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C5Z6L8|A0A5C5Z6L8_9BACT Translocation and assembly module TamA OS=Planctomycetes bacterium CA13 OX=2527986 GN=tamA PE=4 SV=1
MM1 pKa = 7.21FNAARR6 pKa = 11.84QRR8 pKa = 11.84SSATQLGNAARR19 pKa = 11.84QRR21 pKa = 11.84SSAVQLGNAARR32 pKa = 11.84QRR34 pKa = 11.84SSAVQLGGTPPKK46 pKa = 10.41LPKK49 pKa = 10.16LRR51 pKa = 11.84LRR53 pKa = 4.23

Molecular weight: 5.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7247

0

7247

2733373

29

12492

377.2

41.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.055 ± 0.031

1.18 ± 0.015

6.412 ± 0.04

5.928 ± 0.03

3.854 ± 0.017

7.639 ± 0.038

2.227 ± 0.018

5.39 ± 0.019

3.948 ± 0.04

9.198 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.345 ± 0.022

3.629 ± 0.027

5.033 ± 0.024

3.864 ± 0.018

6.26 ± 0.035

6.873 ± 0.029

5.821 ± 0.049

7.194 ± 0.029

1.568 ± 0.017

2.578 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski