Amino acid dipepetide frequency for Planctomycetes bacterium CA13

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.436AlaAla: 9.436 ± 0.093	1.106AlaCys: 1.106 ± 0.023	5.874AlaAsp: 5.874 ± 0.054	5.87AlaGlu: 5.87 ± 0.062	3.341AlaPhe: 3.341 ± 0.037	6.814AlaGly: 6.814 ± 0.078	1.448AlaHis: 1.448 ± 0.03	6.021AlaIle: 6.021 ± 0.053	4.26AlaLys: 4.26 ± 0.062	7.088AlaLeu: 7.088 ± 0.055	2.704AlaMet: 2.704 ± 0.037	3.51AlaAsn: 3.51 ± 0.045	3.538AlaPro: 3.538 ± 0.049	2.831AlaGln: 2.831 ± 0.039	4.495AlaArg: 4.495 ± 0.056	6.527AlaSer: 6.527 ± 0.067	5.789AlaThr: 5.789 ± 0.079	6.569AlaVal: 6.569 ± 0.061	1.264AlaTrp: 1.264 ± 0.026	2.063AlaTyr: 2.063 ± 0.034	0.002AlaXaa: 0.002 ± 0.001
Cys	0.779CysAla: 0.779 ± 0.021	0.233CysCys: 0.233 ± 0.01	0.81CysAsp: 0.81 ± 0.021	0.765CysGlu: 0.765 ± 0.023	0.517CysPhe: 0.517 ± 0.015	1.13CysGly: 1.13 ± 0.028	0.394CysHis: 0.394 ± 0.016	0.545CysIle: 0.545 ± 0.016	0.378CysLys: 0.378 ± 0.012	1.114CysLeu: 1.114 ± 0.028	0.239CysMet: 0.239 ± 0.009	0.379CysAsn: 0.379 ± 0.013	0.59CysPro: 0.59 ± 0.017	0.437CysGln: 0.437 ± 0.012	0.762CysArg: 0.762 ± 0.021	0.756CysSer: 0.756 ± 0.018	0.551CysThr: 0.551 ± 0.018	0.868CysVal: 0.868 ± 0.022	0.194CysTrp: 0.194 ± 0.009	0.354CysTyr: 0.354 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	6.309AspAla: 6.309 ± 0.072	0.698AspCys: 0.698 ± 0.021	4.26AspAsp: 4.26 ± 0.062	4.112AspGlu: 4.112 ± 0.044	2.559AspPhe: 2.559 ± 0.036	5.853AspGly: 5.853 ± 0.095	1.505AspHis: 1.505 ± 0.026	2.821AspIle: 2.821 ± 0.034	1.995AspLys: 1.995 ± 0.03	5.593AspLeu: 5.593 ± 0.052	1.089AspMet: 1.089 ± 0.022	2.019AspAsn: 2.019 ± 0.037	3.695AspPro: 3.695 ± 0.052	2.639AspGln: 2.639 ± 0.033	4.238AspArg: 4.238 ± 0.048	4.244AspSer: 4.244 ± 0.055	3.01AspThr: 3.01 ± 0.06	4.512AspVal: 4.512 ± 0.063	1.222AspTrp: 1.222 ± 0.023	1.749AspTyr: 1.749 ± 0.026	0.001AspXaa: 0.001 ± 0.001
Glu	5.334GluAla: 5.334 ± 0.055	0.533GluCys: 0.533 ± 0.015	2.838GluAsp: 2.838 ± 0.039	3.052GluGlu: 3.052 ± 0.049	2.27GluPhe: 2.27 ± 0.03	3.645GluGly: 3.645 ± 0.041	1.351GluHis: 1.351 ± 0.026	3.579GluIle: 3.579 ± 0.038	2.801GluLys: 2.801 ± 0.041	6.287GluLeu: 6.287 ± 0.061	1.648GluMet: 1.648 ± 0.028	2.334GluAsn: 2.334 ± 0.029	2.764GluPro: 2.764 ± 0.039	2.801GluGln: 2.801 ± 0.043	3.646GluArg: 3.646 ± 0.044	4.534GluSer: 4.534 ± 0.047	3.772GluThr: 3.772 ± 0.043	3.936GluVal: 3.936 ± 0.039	0.731GluTrp: 0.731 ± 0.017	1.421GluTyr: 1.421 ± 0.026	0.0GluXaa: 0.0 ± 0.0
Phe	3.868PheAla: 3.868 ± 0.042	0.567PheCys: 0.567 ± 0.016	3.138PheAsp: 3.138 ± 0.042	2.313PheGlu: 2.313 ± 0.03	1.632PhePhe: 1.632 ± 0.028	3.424PheGly: 3.424 ± 0.039	0.878PheHis: 0.878 ± 0.02	1.558PheIle: 1.558 ± 0.022	1.159PheLys: 1.159 ± 0.024	3.326PheLeu: 3.326 ± 0.039	0.71PheMet: 0.71 ± 0.018	1.33PheAsn: 1.33 ± 0.024	1.65PhePro: 1.65 ± 0.028	1.291PheGln: 1.291 ± 0.022	2.315PheArg: 2.315 ± 0.032	2.662PheSer: 2.662 ± 0.037	2.112PheThr: 2.112 ± 0.034	3.015PheVal: 3.015 ± 0.038	0.59PheTrp: 0.59 ± 0.015	1.007PheTyr: 1.007 ± 0.022	0.0PheXaa: 0.0 ± 0.0
Gly	5.463GlyAla: 5.463 ± 0.075	1.039GlyCys: 1.039 ± 0.028	4.992GlyAsp: 4.992 ± 0.079	4.494GlyGlu: 4.494 ± 0.044	3.165GlyPhe: 3.165 ± 0.042	6.336GlyGly: 6.336 ± 0.102	1.736GlyHis: 1.736 ± 0.03	4.255GlyIle: 4.255 ± 0.042	3.943GlyLys: 3.943 ± 0.05	6.493GlyLeu: 6.493 ± 0.056	1.986GlyMet: 1.986 ± 0.032	3.078GlyAsn: 3.078 ± 0.068	3.073GlyPro: 3.073 ± 0.038	2.94GlyGln: 2.94 ± 0.039	4.631GlyArg: 4.631 ± 0.044	5.063GlySer: 5.063 ± 0.077	4.658GlyThr: 4.658 ± 0.087	5.416GlyVal: 5.416 ± 0.055	1.332GlyTrp: 1.332 ± 0.027	2.301GlyTyr: 2.301 ± 0.03	0.0GlyXaa: 0.0 ± 0.0
His	1.914HisAla: 1.914 ± 0.03	0.353HisCys: 0.353 ± 0.013	1.384HisAsp: 1.384 ± 0.028	1.177HisGlu: 1.177 ± 0.023	1.001HisPhe: 1.001 ± 0.021	1.802HisGly: 1.802 ± 0.029	0.718HisHis: 0.718 ± 0.017	0.891HisIle: 0.891 ± 0.019	0.598HisLys: 0.598 ± 0.018	2.189HisLeu: 2.189 ± 0.035	0.375HisMet: 0.375 ± 0.012	0.707HisAsn: 0.707 ± 0.019	1.377HisPro: 1.377 ± 0.024	0.936HisGln: 0.936 ± 0.023	1.653HisArg: 1.653 ± 0.032	1.435HisSer: 1.435 ± 0.025	0.992HisThr: 0.992 ± 0.019	1.564HisVal: 1.564 ± 0.027	0.479HisTrp: 0.479 ± 0.014	0.726HisTyr: 0.726 ± 0.02	0.0HisXaa: 0.0 ± 0.0
Ile	6.103IleAla: 6.103 ± 0.058	0.672IleCys: 0.672 ± 0.019	4.49IleAsp: 4.49 ± 0.054	4.113IleGlu: 4.113 ± 0.042	1.58IlePhe: 1.58 ± 0.026	4.595IleGly: 4.595 ± 0.048	1.23IleHis: 1.23 ± 0.021	2.041IleIle: 2.041 ± 0.029	1.707IleLys: 1.707 ± 0.03	4.123IleLeu: 4.123 ± 0.047	0.783IleMet: 0.783 ± 0.019	1.815IleAsn: 1.815 ± 0.033	2.701IlePro: 2.701 ± 0.031	1.913IleGln: 1.913 ± 0.025	3.617IleArg: 3.617 ± 0.036	3.352IleSer: 3.352 ± 0.039	2.92IleThr: 2.92 ± 0.056	4.263IleVal: 4.263 ± 0.035	0.628IleTrp: 0.628 ± 0.016	1.25IleTyr: 1.25 ± 0.024	0.001IleXaa: 0.001 ± 0.0
Lys	3.297LysAla: 3.297 ± 0.046	0.355LysCys: 0.355 ± 0.012	2.032LysAsp: 2.032 ± 0.03	2.144LysGlu: 2.144 ± 0.039	1.28LysPhe: 1.28 ± 0.025	2.396LysGly: 2.396 ± 0.04	0.962LysHis: 0.962 ± 0.024	2.079LysIle: 2.079 ± 0.027	2.046LysLys: 2.046 ± 0.046	3.89LysLeu: 3.89 ± 0.049	1.079LysMet: 1.079 ± 0.023	1.431LysAsn: 1.431 ± 0.024	2.496LysPro: 2.496 ± 0.04	1.908LysGln: 1.908 ± 0.033	2.667LysArg: 2.667 ± 0.038	2.653LysSer: 2.653 ± 0.038	2.526LysThr: 2.526 ± 0.037	2.531LysVal: 2.531 ± 0.032	0.684LysTrp: 0.684 ± 0.019	1.024LysTyr: 1.024 ± 0.023	0.001LysXaa: 0.001 ± 0.0
Leu	9.374LeuAla: 9.374 ± 0.078	1.177LeuCys: 1.177 ± 0.026	5.938LeuAsp: 5.938 ± 0.052	4.971LeuGlu: 4.971 ± 0.046	3.476LeuPhe: 3.476 ± 0.039	6.718LeuGly: 6.718 ± 0.053	1.963LeuHis: 1.963 ± 0.029	4.67LeuIle: 4.67 ± 0.048	3.52LeuLys: 3.52 ± 0.05	8.805LeuLeu: 8.805 ± 0.076	2.039LeuMet: 2.039 ± 0.032	2.96LeuAsn: 2.96 ± 0.035	5.001LeuPro: 5.001 ± 0.048	3.264LeuGln: 3.264 ± 0.038	5.993LeuArg: 5.993 ± 0.062	6.641LeuSer: 6.641 ± 0.06	5.241LeuThr: 5.241 ± 0.058	6.804LeuVal: 6.804 ± 0.058	1.237LeuTrp: 1.237 ± 0.023	2.187LeuTyr: 2.187 ± 0.03	0.001LeuXaa: 0.001 ± 0.001
Met	2.099MetAla: 2.099 ± 0.031	0.22MetCys: 0.22 ± 0.011	1.182MetAsp: 1.182 ± 0.02	1.127MetGlu: 1.127 ± 0.022	0.77MetPhe: 0.77 ± 0.018	1.558MetGly: 1.558 ± 0.033	0.531MetHis: 0.531 ± 0.016	1.441MetIle: 1.441 ± 0.025	1.155MetLys: 1.155 ± 0.022	2.387MetLeu: 2.387 ± 0.035	0.689MetMet: 0.689 ± 0.019	0.993MetAsn: 0.993 ± 0.022	1.37MetPro: 1.37 ± 0.025	1.01MetGln: 1.01 ± 0.019	1.483MetArg: 1.483 ± 0.028	1.534MetSer: 1.534 ± 0.025	1.572MetThr: 1.572 ± 0.026	1.649MetVal: 1.649 ± 0.029	0.259MetTrp: 0.259 ± 0.01	0.415MetTyr: 0.415 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	3.27AsnAla: 3.27 ± 0.044	0.375AsnCys: 0.375 ± 0.014	2.437AsnAsp: 2.437 ± 0.053	2.197AsnGlu: 2.197 ± 0.028	1.256AsnPhe: 1.256 ± 0.023	3.237AsnGly: 3.237 ± 0.056	0.91AsnHis: 0.91 ± 0.017	1.611AsnIle: 1.611 ± 0.025	1.073AsnLys: 1.073 ± 0.022	3.178AsnLeu: 3.178 ± 0.037	0.646AsnMet: 0.646 ± 0.016	1.295AsnAsn: 1.295 ± 0.034	2.172AsnPro: 2.172 ± 0.028	1.641AsnGln: 1.641 ± 0.027	2.563AsnArg: 2.563 ± 0.035	2.252AsnSer: 2.252 ± 0.037	1.952AsnThr: 1.952 ± 0.043	2.641AsnVal: 2.641 ± 0.044	0.618AsnTrp: 0.618 ± 0.015	0.967AsnTyr: 0.967 ± 0.019	0.0AsnXaa: 0.0 ± 0.0
Pro	4.346ProAla: 4.346 ± 0.051	0.41ProCys: 0.41 ± 0.014	3.278ProAsp: 3.278 ± 0.04	3.463ProGlu: 3.463 ± 0.047	1.953ProPhe: 1.953 ± 0.032	3.448ProGly: 3.448 ± 0.04	1.09ProHis: 1.09 ± 0.022	2.92ProIle: 2.92 ± 0.04	2.178ProLys: 2.178 ± 0.038	4.326ProLeu: 4.326 ± 0.043	1.248ProMet: 1.248 ± 0.021	2.264ProAsn: 2.264 ± 0.034	2.424ProPro: 2.424 ± 0.041	1.794ProGln: 1.794 ± 0.03	2.475ProArg: 2.475 ± 0.033	3.84ProSer: 3.84 ± 0.049	3.255ProThr: 3.255 ± 0.041	3.555ProVal: 3.555 ± 0.039	0.779ProTrp: 0.779 ± 0.021	1.283ProTyr: 1.283 ± 0.026	0.0ProXaa: 0.0 ± 0.0
Gln	3.43GlnAla: 3.43 ± 0.043	0.405GlnCys: 0.405 ± 0.013	1.663GlnAsp: 1.663 ± 0.026	1.738GlnGlu: 1.738 ± 0.03	1.537GlnPhe: 1.537 ± 0.021	2.378GlnGly: 2.378 ± 0.031	0.872GlnHis: 0.872 ± 0.018	2.256GlnIle: 2.256 ± 0.03	1.44GlnLys: 1.44 ± 0.025	4.011GlnLeu: 4.011 ± 0.046	1.071GlnMet: 1.071 ± 0.021	1.321GlnAsn: 1.321 ± 0.022	2.038GlnPro: 2.038 ± 0.03	1.879GlnGln: 1.879 ± 0.038	2.676GlnArg: 2.676 ± 0.037	2.988GlnSer: 2.988 ± 0.036	2.58GlnThr: 2.58 ± 0.034	2.514GlnVal: 2.514 ± 0.032	0.82GlnTrp: 0.82 ± 0.017	1.022GlnTyr: 1.022 ± 0.024	0.0GlnXaa: 0.0 ± 0.0
Arg	4.308ArgAla: 4.308 ± 0.042	0.804ArgCys: 0.804 ± 0.021	3.773ArgAsp: 3.773 ± 0.042	3.705ArgGlu: 3.705 ± 0.048	2.902ArgPhe: 2.902 ± 0.038	4.07ArgGly: 4.07 ± 0.049	1.41ArgHis: 1.41 ± 0.027	3.808ArgIle: 3.808 ± 0.042	2.467ArgLys: 2.467 ± 0.04	6.409ArgLeu: 6.409 ± 0.069	1.713ArgMet: 1.713 ± 0.031	2.179ArgAsn: 2.179 ± 0.028	2.893ArgPro: 2.893 ± 0.039	2.549ArgGln: 2.549 ± 0.037	4.634ArgArg: 4.634 ± 0.062	4.297ArgSer: 4.297 ± 0.047	3.228ArgThr: 3.228 ± 0.036	4.306ArgVal: 4.306 ± 0.044	1.212ArgTrp: 1.212 ± 0.022	1.933ArgTyr: 1.933 ± 0.031	0.0ArgXaa: 0.0 ± 0.0
Ser	5.508SerAla: 5.508 ± 0.052	0.705SerCys: 0.705 ± 0.018	4.682SerAsp: 4.682 ± 0.057	4.261SerGlu: 4.261 ± 0.043	2.587SerPhe: 2.587 ± 0.034	5.844SerGly: 5.844 ± 0.078	1.473SerHis: 1.473 ± 0.026	3.927SerIle: 3.927 ± 0.04	2.602SerLys: 2.602 ± 0.038	6.664SerLeu: 6.664 ± 0.056	1.615SerMet: 1.615 ± 0.027	2.518SerAsn: 2.518 ± 0.041	3.6SerPro: 3.6 ± 0.044	2.539SerGln: 2.539 ± 0.031	4.151SerArg: 4.151 ± 0.052	4.622SerSer: 4.622 ± 0.057	3.784SerThr: 3.784 ± 0.055	5.106SerVal: 5.106 ± 0.055	0.961SerTrp: 0.961 ± 0.019	1.577SerTyr: 1.577 ± 0.024	0.0SerXaa: 0.0 ± 0.0
Thr	5.236ThrAla: 5.236 ± 0.075	0.568ThrCys: 0.568 ± 0.016	3.684ThrAsp: 3.684 ± 0.062	3.166ThrGlu: 3.166 ± 0.035	2.204ThrPhe: 2.204 ± 0.042	4.697ThrGly: 4.697 ± 0.077	1.199ThrHis: 1.199 ± 0.021	3.718ThrIle: 3.718 ± 0.059	2.128ThrLys: 2.128 ± 0.032	5.714ThrLeu: 5.714 ± 0.057	1.293ThrMet: 1.293 ± 0.023	2.083ThrAsn: 2.083 ± 0.043	3.372ThrPro: 3.372 ± 0.042	1.976ThrGln: 1.976 ± 0.035	3.17ThrArg: 3.17 ± 0.044	3.811ThrSer: 3.811 ± 0.052	3.547ThrThr: 3.547 ± 0.063	4.335ThrVal: 4.335 ± 0.08	0.85ThrTrp: 0.85 ± 0.018	1.463ThrTyr: 1.463 ± 0.035	0.0ThrXaa: 0.0 ± 0.0
Val	7.153ValAla: 7.153 ± 0.063	0.995ValCys: 0.995 ± 0.024	4.953ValAsp: 4.953 ± 0.06	4.307ValGlu: 4.307 ± 0.044	2.767ValPhe: 2.767 ± 0.036	5.297ValGly: 5.297 ± 0.048	1.432ValHis: 1.432 ± 0.024	3.874ValIle: 3.874 ± 0.042	2.474ValLys: 2.474 ± 0.036	6.467ValLeu: 6.467 ± 0.051	1.618ValMet: 1.618 ± 0.026	2.465ValAsn: 2.465 ± 0.042	3.478ValPro: 3.478 ± 0.039	2.405ValGln: 2.405 ± 0.029	4.359ValArg: 4.359 ± 0.039	4.9ValSer: 4.9 ± 0.055	4.494ValThr: 4.494 ± 0.09	5.696ValVal: 5.696 ± 0.052	1.038ValTrp: 1.038 ± 0.021	1.763ValTyr: 1.763 ± 0.028	0.0ValXaa: 0.0 ± 0.0
Trp	1.1TrpAla: 1.1 ± 0.02	0.215TrpCys: 0.215 ± 0.008	0.935TrpAsp: 0.935 ± 0.023	0.757TrpGlu: 0.757 ± 0.018	0.65TrpPhe: 0.65 ± 0.016	1.015TrpGly: 1.015 ± 0.02	0.482TrpHis: 0.482 ± 0.013	0.927TrpIle: 0.927 ± 0.022	0.781TrpLys: 0.781 ± 0.02	1.56TrpLeu: 1.56 ± 0.026	0.46TrpMet: 0.46 ± 0.014	0.711TrpAsn: 0.711 ± 0.018	0.752TrpPro: 0.752 ± 0.019	0.722TrpGln: 0.722 ± 0.018	0.998TrpArg: 0.998 ± 0.021	1.01TrpSer: 1.01 ± 0.02	0.935TrpThr: 0.935 ± 0.021	0.932TrpVal: 0.932 ± 0.022	0.288TrpTrp: 0.288 ± 0.011	0.445TrpTyr: 0.445 ± 0.012	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.223TyrAla: 2.223 ± 0.028	0.365TyrCys: 0.365 ± 0.011	1.779TyrAsp: 1.779 ± 0.036	1.545TyrGlu: 1.545 ± 0.026	1.095TyrPhe: 1.095 ± 0.025	2.136TyrGly: 2.136 ± 0.035	0.688TyrHis: 0.688 ± 0.017	0.955TyrIle: 0.955 ± 0.018	0.78TyrLys: 0.78 ± 0.019	2.355TyrLeu: 2.355 ± 0.031	0.439TyrMet: 0.439 ± 0.012	0.895TyrAsn: 0.895 ± 0.02	1.344TyrPro: 1.344 ± 0.026	1.165TyrGln: 1.165 ± 0.022	2.069TyrArg: 2.069 ± 0.033	1.606TyrSer: 1.606 ± 0.025	1.298TyrThr: 1.298 ± 0.029	1.724TyrVal: 1.724 ± 0.028	0.488TyrTrp: 0.488 ± 0.014	0.832TyrTyr: 0.832 ± 0.02	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.001XaaHis: 0.001 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.001XaaSer: 0.001 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.031XaaXaa: 0.031 ± 0.008

Statistics based on 7247 proteins (2733373 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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