Melioribacter roseus (strain JCM 17771 / P3M-2)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Ignavibacteriae; Ignavibacteria; Ignavibacteriales; Melioribacteraceae; Melioribacter; Melioribacter roseus

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2833 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I7A7Y2|I7A7Y2_MELRP PSCyt1 domain-containing protein OS=Melioribacter roseus (strain JCM 17771 / P3M-2) OX=1191523 GN=MROS_2746 PE=4 SV=1
MM1 pKa = 7.32KK2 pKa = 10.4AFYY5 pKa = 10.16KK6 pKa = 10.42IFAILILSYY15 pKa = 11.11GFIAAQEE22 pKa = 4.13LSVGADD28 pKa = 3.17VVSRR32 pKa = 11.84YY33 pKa = 7.17IWRR36 pKa = 11.84GADD39 pKa = 3.19LGNNNPSIQPTIEE52 pKa = 3.95LSSGGFAAGFWGAYY66 pKa = 8.66PMSDD70 pKa = 2.92PAGLNEE76 pKa = 3.7IDD78 pKa = 5.19FYY80 pKa = 11.79ASYY83 pKa = 11.09SFSLGKK89 pKa = 10.58AGDD92 pKa = 3.75LSIGFTDD99 pKa = 3.48YY100 pKa = 10.87MNPNSGTKK108 pKa = 9.44IGNFNNYY115 pKa = 9.59DD116 pKa = 3.65DD117 pKa = 5.68AEE119 pKa = 4.75GPGAHH124 pKa = 7.08FIEE127 pKa = 5.19LNVGYY132 pKa = 9.83GGSEE136 pKa = 4.06SFPVSISFNYY146 pKa = 9.76FLYY149 pKa = 10.67NVEE152 pKa = 4.21NNPIYY157 pKa = 11.03VEE159 pKa = 4.37LGYY162 pKa = 9.0STSVKK167 pKa = 10.43DD168 pKa = 3.59VSLDD172 pKa = 3.51LFLGATPGEE181 pKa = 4.07DD182 pKa = 2.77ALYY185 pKa = 10.93YY186 pKa = 10.11GTDD189 pKa = 3.0SFNIINLGITAGKK202 pKa = 9.25SIKK205 pKa = 10.23ISDD208 pKa = 3.93SFEE211 pKa = 4.39LPVFGSVILNPATEE225 pKa = 4.24DD226 pKa = 3.16LFYY229 pKa = 11.27VIGISLL235 pKa = 3.62

Molecular weight: 25.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I6Z307|I6Z307_MELRP ATP synthase gamma chain OS=Melioribacter roseus (strain JCM 17771 / P3M-2) OX=1191523 GN=atpG PE=3 SV=1
MM1 pKa = 6.76VRR3 pKa = 11.84KK4 pKa = 9.68GRR6 pKa = 11.84EE7 pKa = 3.77VVTSKK12 pKa = 11.13NKK14 pKa = 10.28APALEE19 pKa = 4.41ACPQKK24 pKa = 10.67RR25 pKa = 11.84GVCTRR30 pKa = 11.84VYY32 pKa = 7.93TTTPKK37 pKa = 10.54KK38 pKa = 10.26PNSALRR44 pKa = 11.84KK45 pKa = 7.18VARR48 pKa = 11.84VRR50 pKa = 11.84LSNGIEE56 pKa = 3.8VTAYY60 pKa = 9.84IPGEE64 pKa = 3.86GHH66 pKa = 6.47NLQEE70 pKa = 4.15HH71 pKa = 6.65SIVLIRR77 pKa = 11.84GGRR80 pKa = 11.84VKK82 pKa = 10.7DD83 pKa = 3.73LPGVRR88 pKa = 11.84YY89 pKa = 9.5HH90 pKa = 7.64IIRR93 pKa = 11.84GTLDD97 pKa = 3.03TSGVEE102 pKa = 3.94DD103 pKa = 3.93RR104 pKa = 11.84KK105 pKa = 10.55QGRR108 pKa = 11.84SKK110 pKa = 11.3YY111 pKa = 8.24GTKK114 pKa = 10.06KK115 pKa = 10.32PKK117 pKa = 10.59AKK119 pKa = 10.49

Molecular weight: 13.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2833

0

2833

1007411

37

2275

355.6

40.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.456 ± 0.038

0.73 ± 0.017

5.592 ± 0.032

7.165 ± 0.054

5.027 ± 0.032

6.636 ± 0.04

1.524 ± 0.016

8.618 ± 0.043

7.849 ± 0.047

9.361 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.068 ± 0.019

5.785 ± 0.042

3.692 ± 0.028

2.484 ± 0.021

4.171 ± 0.029

6.264 ± 0.033

4.891 ± 0.035

6.18 ± 0.036

1.056 ± 0.017

4.452 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski