Microbacterium phage Casend

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Squashvirus; unclassified Squashvirus

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 106 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G9A0C8|A0A7G9A0C8_9CAUD Uncharacterized protein OS=Microbacterium phage Casend OX=2759454 GN=100 PE=4 SV=1
MM1 pKa = 7.65SIFDD5 pKa = 3.69IVGYY9 pKa = 7.71TYY11 pKa = 10.94KK12 pKa = 10.56AQQYY16 pKa = 8.35TPDD19 pKa = 3.56GVIAALATGPGQDD32 pKa = 3.19FDD34 pKa = 3.79GWALGDD40 pKa = 3.88GVDD43 pKa = 4.1MSVEE47 pKa = 4.12GNLNEE52 pKa = 3.89LAAAFGIDD60 pKa = 3.48RR61 pKa = 11.84ADD63 pKa = 3.38EE64 pKa = 4.19TSFDD68 pKa = 3.15TDD70 pKa = 3.49YY71 pKa = 11.05FPKK74 pKa = 10.29VVLRR78 pKa = 11.84INVEE82 pKa = 3.65DD83 pKa = 4.49DD84 pKa = 3.74EE85 pKa = 4.92YY86 pKa = 11.53FEE88 pKa = 5.4NEE90 pKa = 4.31HH91 pKa = 6.54GDD93 pKa = 4.34HH94 pKa = 6.88VLLDD98 pKa = 3.64NDD100 pKa = 3.72

Molecular weight: 11.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G9A0D4|A0A7G9A0D4_9CAUD Uncharacterized protein OS=Microbacterium phage Casend OX=2759454 GN=106 PE=4 SV=1
MM1 pKa = 7.25TSLRR5 pKa = 11.84KK6 pKa = 8.35ITKK9 pKa = 9.96LEE11 pKa = 3.81AAAYY15 pKa = 8.04QASQAATQAVVLARR29 pKa = 11.84MDD31 pKa = 3.67GATEE35 pKa = 4.0AEE37 pKa = 4.18IARR40 pKa = 11.84LSRR43 pKa = 11.84RR44 pKa = 11.84AQAHH48 pKa = 4.6RR49 pKa = 11.84TQWLGLASRR58 pKa = 11.84LEE60 pKa = 4.24LADD63 pKa = 3.4QRR65 pKa = 11.84AEE67 pKa = 4.07RR68 pKa = 11.84VHH70 pKa = 6.24SS71 pKa = 3.9

Molecular weight: 7.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

106

0

106

19815

29

1070

186.9

20.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.728 ± 0.303

0.838 ± 0.119

6.445 ± 0.185

6.712 ± 0.362

2.71 ± 0.142

8.483 ± 0.273

1.746 ± 0.179

4.779 ± 0.168

2.766 ± 0.228

7.706 ± 0.278

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.236 ± 0.125

2.826 ± 0.159

5.587 ± 0.349

3.74 ± 0.364

6.904 ± 0.271

5.683 ± 0.291

7.111 ± 0.407

7.58 ± 0.247

1.867 ± 0.145

2.554 ± 0.137
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski