Amino acid dipepetide frequency for Microbacterium phage Casend

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.614AlaAla: 17.614 ± 1.405
0.656AlaCys: 0.656 ± 0.225
7.924AlaAsp: 7.924 ± 0.82
7.974AlaGlu: 7.974 ± 0.894
2.725AlaPhe: 2.725 ± 0.346
8.025AlaGly: 8.025 ± 0.764
1.968AlaHis: 1.968 ± 0.414
5.451AlaIle: 5.451 ± 0.549
4.138AlaLys: 4.138 ± 0.53
9.741AlaLeu: 9.741 ± 0.807
2.927AlaMet: 2.927 ± 0.358
3.129AlaAsn: 3.129 ± 0.481
5.955AlaPro: 5.955 ± 0.696
4.239AlaGln: 4.239 ± 0.545
7.217AlaArg: 7.217 ± 0.595
5.955AlaSer: 5.955 ± 0.742
8.025AlaThr: 8.025 ± 0.832
8.832AlaVal: 8.832 ± 0.734
2.019AlaTrp: 2.019 ± 0.328
2.725AlaTyr: 2.725 ± 0.382
0.0AlaXaa: 0.0 ± 0.0
Cys
0.757CysAla: 0.757 ± 0.206
0.101CysCys: 0.101 ± 0.075
0.707CysAsp: 0.707 ± 0.216
0.555CysGlu: 0.555 ± 0.182
0.404CysPhe: 0.404 ± 0.18
0.908CysGly: 0.908 ± 0.266
0.151CysHis: 0.151 ± 0.08
0.252CysIle: 0.252 ± 0.109
0.151CysLys: 0.151 ± 0.093
0.505CysLeu: 0.505 ± 0.188
0.0CysMet: 0.0 ± 0.0
0.101CysAsn: 0.101 ± 0.069
0.808CysPro: 0.808 ± 0.235
0.151CysGln: 0.151 ± 0.081
0.606CysArg: 0.606 ± 0.233
0.505CysSer: 0.505 ± 0.281
0.656CysThr: 0.656 ± 0.195
0.707CysVal: 0.707 ± 0.181
0.101CysTrp: 0.101 ± 0.074
0.252CysTyr: 0.252 ± 0.102
0.0CysXaa: 0.0 ± 0.0
Asp
7.873AspAla: 7.873 ± 0.687
0.808AspCys: 0.808 ± 0.186
3.735AspAsp: 3.735 ± 0.455
4.492AspGlu: 4.492 ± 0.574
2.271AspPhe: 2.271 ± 0.303
7.217AspGly: 7.217 ± 0.619
1.363AspHis: 1.363 ± 0.296
2.17AspIle: 2.17 ± 0.325
1.766AspLys: 1.766 ± 0.342
5.501AspLeu: 5.501 ± 0.583
1.413AspMet: 1.413 ± 0.228
1.817AspAsn: 1.817 ± 0.371
4.239AspPro: 4.239 ± 0.4
1.918AspGln: 1.918 ± 0.342
4.189AspArg: 4.189 ± 0.414
2.624AspSer: 2.624 ± 0.355
3.381AspThr: 3.381 ± 0.4
4.441AspVal: 4.441 ± 0.441
1.565AspTrp: 1.565 ± 0.275
1.665AspTyr: 1.665 ± 0.409
0.0AspXaa: 0.0 ± 0.0
Glu
7.268GluAla: 7.268 ± 0.774
0.454GluCys: 0.454 ± 0.16
3.886GluAsp: 3.886 ± 0.543
4.29GluGlu: 4.29 ± 0.627
1.918GluPhe: 1.918 ± 0.301
4.34GluGly: 4.34 ± 0.629
1.312GluHis: 1.312 ± 0.281
3.482GluIle: 3.482 ± 0.483
2.624GluLys: 2.624 ± 0.645
5.602GluLeu: 5.602 ± 0.6
1.312GluMet: 1.312 ± 0.263
1.817GluAsn: 1.817 ± 0.312
4.189GluPro: 4.189 ± 0.69
3.886GluGln: 3.886 ± 0.585
5.552GluArg: 5.552 ± 0.701
2.927GluSer: 2.927 ± 0.408
3.684GluThr: 3.684 ± 0.374
5.804GluVal: 5.804 ± 0.561
1.262GluTrp: 1.262 ± 0.244
1.514GluTyr: 1.514 ± 0.246
0.0GluXaa: 0.0 ± 0.0
Phe
2.776PheAla: 2.776 ± 0.384
0.252PheCys: 0.252 ± 0.121
3.028PheAsp: 3.028 ± 0.443
1.766PheGlu: 1.766 ± 0.307
0.252PhePhe: 0.252 ± 0.099
2.978PheGly: 2.978 ± 0.435
0.353PheHis: 0.353 ± 0.157
1.161PheIle: 1.161 ± 0.22
0.707PheLys: 0.707 ± 0.186
1.817PheLeu: 1.817 ± 0.404
0.555PheMet: 0.555 ± 0.203
0.454PheAsn: 0.454 ± 0.139
1.716PhePro: 1.716 ± 0.337
0.454PheGln: 0.454 ± 0.126
1.665PheArg: 1.665 ± 0.279
1.766PheSer: 1.766 ± 0.347
2.523PheThr: 2.523 ± 0.388
1.413PheVal: 1.413 ± 0.272
0.303PheTrp: 0.303 ± 0.152
1.161PheTyr: 1.161 ± 0.25
0.0PheXaa: 0.0 ± 0.0
Gly
8.227GlyAla: 8.227 ± 0.816
0.858GlyCys: 0.858 ± 0.267
5.602GlyAsp: 5.602 ± 0.577
5.047GlyGlu: 5.047 ± 0.627
2.978GlyPhe: 2.978 ± 0.38
7.015GlyGly: 7.015 ± 0.6
1.413GlyHis: 1.413 ± 0.317
2.877GlyIle: 2.877 ± 0.429
3.331GlyLys: 3.331 ± 0.434
6.258GlyLeu: 6.258 ± 0.67
2.221GlyMet: 2.221 ± 0.303
2.574GlyAsn: 2.574 ± 0.364
3.331GlyPro: 3.331 ± 0.369
3.18GlyGln: 3.18 ± 0.436
6.46GlyArg: 6.46 ± 0.75
4.946GlySer: 4.946 ± 0.576
6.309GlyThr: 6.309 ± 0.894
7.268GlyVal: 7.268 ± 0.642
2.069GlyTrp: 2.069 ± 0.429
2.877GlyTyr: 2.877 ± 0.394
0.0GlyXaa: 0.0 ± 0.0
His
2.271HisAla: 2.271 ± 0.369
0.151HisCys: 0.151 ± 0.09
0.959HisAsp: 0.959 ± 0.237
1.413HisGlu: 1.413 ± 0.302
0.555HisPhe: 0.555 ± 0.162
1.464HisGly: 1.464 ± 0.291
0.606HisHis: 0.606 ± 0.211
0.606HisIle: 0.606 ± 0.206
0.151HisLys: 0.151 ± 0.086
1.766HisLeu: 1.766 ± 0.323
0.252HisMet: 0.252 ± 0.101
0.505HisAsn: 0.505 ± 0.15
1.11HisPro: 1.11 ± 0.273
0.303HisGln: 0.303 ± 0.129
1.464HisArg: 1.464 ± 0.355
1.161HisSer: 1.161 ± 0.297
0.656HisThr: 0.656 ± 0.202
1.262HisVal: 1.262 ± 0.3
0.353HisTrp: 0.353 ± 0.131
0.454HisTyr: 0.454 ± 0.131
0.0HisXaa: 0.0 ± 0.0
Ile
5.653IleAla: 5.653 ± 0.607
0.353IleCys: 0.353 ± 0.163
3.18IleAsp: 3.18 ± 0.371
3.381IleGlu: 3.381 ± 0.393
1.211IlePhe: 1.211 ± 0.295
4.29IleGly: 4.29 ± 0.576
1.06IleHis: 1.06 ± 0.295
2.423IleIle: 2.423 ± 0.329
0.454IleLys: 0.454 ± 0.161
2.675IleLeu: 2.675 ± 0.345
0.707IleMet: 0.707 ± 0.182
1.413IleAsn: 1.413 ± 0.25
2.826IlePro: 2.826 ± 0.453
1.464IleGln: 1.464 ± 0.267
3.23IleArg: 3.23 ± 0.408
2.877IleSer: 2.877 ± 0.362
4.542IleThr: 4.542 ± 0.443
4.34IleVal: 4.34 ± 0.429
0.908IleTrp: 0.908 ± 0.21
0.808IleTyr: 0.808 ± 0.185
0.0IleXaa: 0.0 ± 0.0
Lys
3.634LysAla: 3.634 ± 0.625
0.151LysCys: 0.151 ± 0.108
1.565LysAsp: 1.565 ± 0.246
1.968LysGlu: 1.968 ± 0.36
0.959LysPhe: 0.959 ± 0.189
1.918LysGly: 1.918 ± 0.323
0.555LysHis: 0.555 ± 0.191
1.312LysIle: 1.312 ± 0.259
0.303LysLys: 0.303 ± 0.135
2.877LysLeu: 2.877 ± 0.429
0.757LysMet: 0.757 ± 0.188
0.757LysAsn: 0.757 ± 0.192
1.565LysPro: 1.565 ± 0.265
1.009LysGln: 1.009 ± 0.22
1.918LysArg: 1.918 ± 0.41
1.615LysSer: 1.615 ± 0.286
1.867LysThr: 1.867 ± 0.34
1.968LysVal: 1.968 ± 0.345
0.404LysTrp: 0.404 ± 0.166
0.555LysTyr: 0.555 ± 0.185
0.0LysXaa: 0.0 ± 0.0
Leu
10.851LeuAla: 10.851 ± 1.075
0.353LeuCys: 0.353 ± 0.134
5.198LeuAsp: 5.198 ± 0.589
5.451LeuGlu: 5.451 ± 0.706
1.665LeuPhe: 1.665 ± 0.308
7.116LeuGly: 7.116 ± 0.707
1.06LeuHis: 1.06 ± 0.295
4.088LeuIle: 4.088 ± 0.576
1.918LeuLys: 1.918 ± 0.294
5.854LeuLeu: 5.854 ± 0.489
1.514LeuMet: 1.514 ± 0.277
2.776LeuAsn: 2.776 ± 0.444
3.583LeuPro: 3.583 ± 0.422
2.019LeuGln: 2.019 ± 0.317
5.249LeuArg: 5.249 ± 0.517
4.996LeuSer: 4.996 ± 0.702
5.955LeuThr: 5.955 ± 0.441
4.845LeuVal: 4.845 ± 0.663
1.211LeuTrp: 1.211 ± 0.246
1.363LeuTyr: 1.363 ± 0.243
0.0LeuXaa: 0.0 ± 0.0
Met
2.574MetAla: 2.574 ± 0.417
0.151MetCys: 0.151 ± 0.083
0.908MetAsp: 0.908 ± 0.217
0.707MetGlu: 0.707 ± 0.217
0.555MetPhe: 0.555 ± 0.14
1.766MetGly: 1.766 ± 0.243
0.303MetHis: 0.303 ± 0.123
1.009MetIle: 1.009 ± 0.22
0.505MetLys: 0.505 ± 0.153
2.019MetLeu: 2.019 ± 0.282
0.303MetMet: 0.303 ± 0.129
0.757MetAsn: 0.757 ± 0.212
1.716MetPro: 1.716 ± 0.309
1.11MetGln: 1.11 ± 0.274
2.019MetArg: 2.019 ± 0.336
2.423MetSer: 2.423 ± 0.457
1.766MetThr: 1.766 ± 0.334
1.312MetVal: 1.312 ± 0.282
0.202MetTrp: 0.202 ± 0.106
0.252MetTyr: 0.252 ± 0.113
0.0MetXaa: 0.0 ± 0.0
Asn
3.533AsnAla: 3.533 ± 0.396
0.202AsnCys: 0.202 ± 0.107
1.514AsnAsp: 1.514 ± 0.289
2.221AsnGlu: 2.221 ± 0.362
0.757AsnPhe: 0.757 ± 0.254
3.23AsnGly: 3.23 ± 0.377
0.404AsnHis: 0.404 ± 0.151
1.11AsnIle: 1.11 ± 0.242
0.404AsnLys: 0.404 ± 0.116
2.322AsnLeu: 2.322 ± 0.576
0.353AsnMet: 0.353 ± 0.142
0.707AsnAsn: 0.707 ± 0.228
2.473AsnPro: 2.473 ± 0.492
1.262AsnGln: 1.262 ± 0.266
1.413AsnArg: 1.413 ± 0.246
1.413AsnSer: 1.413 ± 0.304
2.069AsnThr: 2.069 ± 0.325
1.615AsnVal: 1.615 ± 0.329
0.606AsnTrp: 0.606 ± 0.185
0.656AsnTyr: 0.656 ± 0.16
0.0AsnXaa: 0.0 ± 0.0
Pro
7.116ProAla: 7.116 ± 0.884
0.404ProCys: 0.404 ± 0.166
3.533ProAsp: 3.533 ± 0.622
5.047ProGlu: 5.047 ± 0.583
1.262ProPhe: 1.262 ± 0.248
4.795ProGly: 4.795 ± 0.673
0.808ProHis: 0.808 ± 0.205
2.423ProIle: 2.423 ± 0.363
1.06ProLys: 1.06 ± 0.227
3.432ProLeu: 3.432 ± 0.383
0.858ProMet: 0.858 ± 0.195
1.867ProAsn: 1.867 ± 0.361
2.675ProPro: 2.675 ± 0.417
2.523ProGln: 2.523 ± 0.789
3.381ProArg: 3.381 ± 0.485
3.684ProSer: 3.684 ± 0.519
4.138ProThr: 4.138 ± 0.644
4.138ProVal: 4.138 ± 0.487
1.06ProTrp: 1.06 ± 0.188
1.665ProTyr: 1.665 ± 0.311
0.0ProXaa: 0.0 ± 0.0
Gln
4.492GlnAla: 4.492 ± 0.614
0.202GlnCys: 0.202 ± 0.106
1.464GlnAsp: 1.464 ± 0.262
2.019GlnGlu: 2.019 ± 0.416
0.908GlnPhe: 0.908 ± 0.216
2.978GlnGly: 2.978 ± 0.649
0.858GlnHis: 0.858 ± 0.219
1.968GlnIle: 1.968 ± 0.336
1.11GlnLys: 1.11 ± 0.282
2.675GlnLeu: 2.675 ± 0.403
1.11GlnMet: 1.11 ± 0.272
0.808GlnAsn: 0.808 ± 0.184
2.12GlnPro: 2.12 ± 0.593
3.432GlnGln: 3.432 ± 1.596
2.927GlnArg: 2.927 ± 0.337
1.867GlnSer: 1.867 ± 0.35
1.918GlnThr: 1.918 ± 0.262
2.574GlnVal: 2.574 ± 0.332
0.959GlnTrp: 0.959 ± 0.192
1.009GlnTyr: 1.009 ± 0.272
0.0GlnXaa: 0.0 ± 0.0
Arg
7.52ArgAla: 7.52 ± 0.522
1.11ArgCys: 1.11 ± 0.298
4.795ArgAsp: 4.795 ± 0.534
4.542ArgGlu: 4.542 ± 0.519
1.615ArgPhe: 1.615 ± 0.266
5.047ArgGly: 5.047 ± 0.588
1.161ArgHis: 1.161 ± 0.268
4.189ArgIle: 4.189 ± 0.533
2.624ArgLys: 2.624 ± 0.359
4.593ArgLeu: 4.593 ± 0.428
2.675ArgMet: 2.675 ± 0.367
1.968ArgAsn: 1.968 ± 0.285
1.918ArgPro: 1.918 ± 0.37
2.322ArgGln: 2.322 ± 0.291
6.107ArgArg: 6.107 ± 0.642
3.987ArgSer: 3.987 ± 0.379
3.735ArgThr: 3.735 ± 0.409
5.552ArgVal: 5.552 ± 0.504
1.11ArgTrp: 1.11 ± 0.255
2.473ArgTyr: 2.473 ± 0.332
0.0ArgXaa: 0.0 ± 0.0
Ser
6.258SerAla: 6.258 ± 0.897
0.505SerCys: 0.505 ± 0.179
3.129SerAsp: 3.129 ± 0.389
3.886SerGlu: 3.886 ± 0.535
1.565SerPhe: 1.565 ± 0.269
5.249SerGly: 5.249 ± 0.515
1.11SerHis: 1.11 ± 0.254
2.069SerIle: 2.069 ± 0.279
1.262SerLys: 1.262 ± 0.268
4.795SerLeu: 4.795 ± 0.53
1.716SerMet: 1.716 ± 0.295
1.363SerAsn: 1.363 ± 0.354
3.18SerPro: 3.18 ± 0.488
1.565SerGln: 1.565 ± 0.323
3.937SerArg: 3.937 ± 0.493
3.432SerSer: 3.432 ± 0.545
5.249SerThr: 5.249 ± 0.633
4.391SerVal: 4.391 ± 0.534
0.505SerTrp: 0.505 ± 0.176
1.665SerTyr: 1.665 ± 0.262
0.0SerXaa: 0.0 ± 0.0
Thr
7.066ThrAla: 7.066 ± 0.696
0.606ThrCys: 0.606 ± 0.193
4.088ThrAsp: 4.088 ± 0.53
4.896ThrGlu: 4.896 ± 0.5
1.968ThrPhe: 1.968 ± 0.236
6.006ThrGly: 6.006 ± 0.757
0.908ThrHis: 0.908 ± 0.273
4.239ThrIle: 4.239 ± 0.63
2.322ThrLys: 2.322 ± 0.369
6.41ThrLeu: 6.41 ± 0.533
1.363ThrMet: 1.363 ± 0.301
1.312ThrAsn: 1.312 ± 0.26
5.35ThrPro: 5.35 ± 0.684
2.423ThrGln: 2.423 ± 0.341
3.684ThrArg: 3.684 ± 0.508
3.836ThrSer: 3.836 ± 0.527
5.804ThrThr: 5.804 ± 0.684
5.703ThrVal: 5.703 ± 0.594
1.413ThrTrp: 1.413 ± 0.24
1.716ThrTyr: 1.716 ± 0.349
0.0ThrXaa: 0.0 ± 0.0
Val
7.318ValAla: 7.318 ± 0.73
0.505ValCys: 0.505 ± 0.173
5.955ValAsp: 5.955 ± 0.527
4.492ValGlu: 4.492 ± 0.406
2.069ValPhe: 2.069 ± 0.37
6.561ValGly: 6.561 ± 0.619
1.413ValHis: 1.413 ± 0.25
4.441ValIle: 4.441 ± 0.362
2.019ValLys: 2.019 ± 0.408
5.501ValLeu: 5.501 ± 0.59
1.665ValMet: 1.665 ± 0.323
2.826ValAsn: 2.826 ± 0.35
4.643ValPro: 4.643 ± 0.71
2.322ValGln: 2.322 ± 0.526
4.744ValArg: 4.744 ± 0.428
4.391ValSer: 4.391 ± 0.507
5.451ValThr: 5.451 ± 0.518
6.006ValVal: 6.006 ± 0.648
1.665ValTrp: 1.665 ± 0.244
1.817ValTyr: 1.817 ± 0.285
0.0ValXaa: 0.0 ± 0.0
Trp
1.867TrpAla: 1.867 ± 0.365
0.353TrpCys: 0.353 ± 0.131
1.009TrpAsp: 1.009 ± 0.184
1.06TrpGlu: 1.06 ± 0.223
0.808TrpPhe: 0.808 ± 0.226
1.262TrpGly: 1.262 ± 0.255
0.202TrpHis: 0.202 ± 0.109
0.908TrpIle: 0.908 ± 0.208
0.404TrpLys: 0.404 ± 0.152
1.312TrpLeu: 1.312 ± 0.28
0.303TrpMet: 0.303 ± 0.13
0.454TrpAsn: 0.454 ± 0.173
0.858TrpPro: 0.858 ± 0.197
0.808TrpGln: 0.808 ± 0.231
1.161TrpArg: 1.161 ± 0.292
1.161TrpSer: 1.161 ± 0.236
2.271TrpThr: 2.271 ± 0.347
1.262TrpVal: 1.262 ± 0.224
0.555TrpTrp: 0.555 ± 0.157
0.656TrpTyr: 0.656 ± 0.177
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.624TyrAla: 2.624 ± 0.374
0.202TyrCys: 0.202 ± 0.101
2.271TyrAsp: 2.271 ± 0.36
1.918TyrGlu: 1.918 ± 0.302
0.656TyrPhe: 0.656 ± 0.182
2.675TyrGly: 2.675 ± 0.44
0.454TyrHis: 0.454 ± 0.138
1.615TyrIle: 1.615 ± 0.329
0.404TyrLys: 0.404 ± 0.163
1.413TyrLeu: 1.413 ± 0.255
0.303TyrMet: 0.303 ± 0.105
0.858TyrAsn: 0.858 ± 0.197
1.615TyrPro: 1.615 ± 0.339
1.009TyrGln: 1.009 ± 0.216
2.12TyrArg: 2.12 ± 0.392
1.262TyrSer: 1.262 ± 0.256
1.11TyrThr: 1.11 ± 0.269
2.372TyrVal: 2.372 ± 0.479
0.404TyrTrp: 0.404 ± 0.133
0.252TyrTyr: 0.252 ± 0.116
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 106 proteins (19815 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski