Halalkaliarchaeum desulfuricum

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales; Halorubraceae; Halalkaliarchaeum

Average proteome isoelectric point is 4.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3224 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A343TGW0|A0A343TGW0_9EURY 50S ribosomal protein L5 OS=Halalkaliarchaeum desulfuricum OX=2055893 GN=rpl5p PE=3 SV=1
MM1 pKa = 7.15EE2 pKa = 5.63RR3 pKa = 11.84SRR5 pKa = 11.84RR6 pKa = 11.84TFVKK10 pKa = 10.35SSLGVVAAGTLAGCAGGDD28 pKa = 3.76PGDD31 pKa = 3.48SDD33 pKa = 3.66GTHH36 pKa = 6.08EE37 pKa = 4.53PEE39 pKa = 4.43EE40 pKa = 4.55TPEE43 pKa = 4.01PTPEE47 pKa = 4.03PTPEE51 pKa = 4.97PDD53 pKa = 3.17THH55 pKa = 7.32SVTLLVEE62 pKa = 4.69GVGEE66 pKa = 4.67DD67 pKa = 4.01YY68 pKa = 11.39DD69 pKa = 5.52HH70 pKa = 7.57EE71 pKa = 6.22DD72 pKa = 5.82DD73 pKa = 5.58EE74 pKa = 7.82DD75 pKa = 5.73DD76 pKa = 4.77DD77 pKa = 4.38NHH79 pKa = 7.88DD80 pKa = 3.94EE81 pKa = 4.77GLDD84 pKa = 3.34EE85 pKa = 4.68HH86 pKa = 8.21AIEE89 pKa = 4.57HH90 pKa = 6.67ACGHH94 pKa = 5.16MQYY97 pKa = 10.84DD98 pKa = 4.07EE99 pKa = 4.76PEE101 pKa = 4.23SLEE104 pKa = 4.43GGSSGDD110 pKa = 3.73DD111 pKa = 3.04APVIHH116 pKa = 6.19TTHH119 pKa = 6.9LPFDD123 pKa = 3.69VTFEE127 pKa = 4.55DD128 pKa = 3.6GGGYY132 pKa = 9.95VVFEE136 pKa = 4.53VDD138 pKa = 5.24DD139 pKa = 5.08HH140 pKa = 8.5DD141 pKa = 6.31DD142 pKa = 4.33DD143 pKa = 6.87HH144 pKa = 9.36DD145 pKa = 5.77DD146 pKa = 4.49DD147 pKa = 6.75HH148 pKa = 9.35DD149 pKa = 6.8DD150 pKa = 5.1DD151 pKa = 5.98DD152 pKa = 6.82HH153 pKa = 9.49DD154 pKa = 6.34DD155 pKa = 4.12DD156 pKa = 6.82HH157 pKa = 9.35DD158 pKa = 6.8DD159 pKa = 5.57DD160 pKa = 5.89DD161 pKa = 6.85HH162 pKa = 9.53DD163 pKa = 7.02DD164 pKa = 5.09DD165 pKa = 6.12DD166 pKa = 6.85HH167 pKa = 9.53DD168 pKa = 7.02DD169 pKa = 5.09DD170 pKa = 6.12DD171 pKa = 6.85HH172 pKa = 9.53DD173 pKa = 6.96DD174 pKa = 4.81DD175 pKa = 5.83DD176 pKa = 6.77HH177 pKa = 7.85DD178 pKa = 4.76DD179 pKa = 3.51SSRR182 pKa = 11.84FGFFTQGGTVEE193 pKa = 4.52VVDD196 pKa = 4.31GQFHH200 pKa = 6.29HH201 pKa = 6.87EE202 pKa = 3.86EE203 pKa = 4.34HH204 pKa = 6.6GVEE207 pKa = 4.08EE208 pKa = 4.69CDD210 pKa = 4.74EE211 pKa = 4.25IDD213 pKa = 3.55AYY215 pKa = 10.73AVAEE219 pKa = 4.2SHH221 pKa = 6.43EE222 pKa = 4.49GTVTIRR228 pKa = 11.84VYY230 pKa = 11.56ADD232 pKa = 2.85

Molecular weight: 25.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A343TH36|A0A343TH36_9EURY Uncharacterized protein OS=Halalkaliarchaeum desulfuricum OX=2055893 GN=AArcSl_0762 PE=4 SV=1
MM1 pKa = 7.72PIEE4 pKa = 3.97AAINAKK10 pKa = 8.2TWLTPYY16 pKa = 10.38SITPQTILKK25 pKa = 8.14SACVAAPTNSKK36 pKa = 10.24NPSIASSTEE45 pKa = 3.66SSVAASRR52 pKa = 11.84TIDD55 pKa = 3.34SANPKK60 pKa = 8.9RR61 pKa = 11.84TTTKK65 pKa = 9.39IVARR69 pKa = 11.84MSPSAIAAIGFRR81 pKa = 11.84GTMPNITSPTVGGVASRR98 pKa = 11.84ASPPVNSTARR108 pKa = 11.84PGSNRR113 pKa = 11.84FATARR118 pKa = 11.84PMTAATVVVTTYY130 pKa = 10.55RR131 pKa = 11.84PTVSPPTFPSFEE143 pKa = 4.19GVSLPTGNQALSLSVRR159 pKa = 11.84LTGVRR164 pKa = 11.84DD165 pKa = 3.93VIPEE169 pKa = 4.11DD170 pKa = 3.85EE171 pKa = 4.3PAA173 pKa = 4.64

Molecular weight: 17.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3224

0

3224

966158

46

3086

299.7

32.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.206 ± 0.055

0.762 ± 0.013

8.221 ± 0.058

9.31 ± 0.064

3.367 ± 0.031

8.58 ± 0.04

2.003 ± 0.021

4.446 ± 0.034

1.932 ± 0.028

8.826 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.711 ± 0.022

2.273 ± 0.024

4.846 ± 0.029

2.403 ± 0.024

6.834 ± 0.046

5.331 ± 0.028

6.177 ± 0.028

8.903 ± 0.047

1.132 ± 0.021

2.737 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski