Amino acid dipepetide frequency for Lysobacter pythonis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.339AlaAla: 17.339 ± 0.205
1.206AlaCys: 1.206 ± 0.049
6.676AlaAsp: 6.676 ± 0.091
7.81AlaGlu: 7.81 ± 0.133
4.138AlaPhe: 4.138 ± 0.068
11.973AlaGly: 11.973 ± 0.166
2.861AlaHis: 2.861 ± 0.075
5.611AlaIle: 5.611 ± 0.087
3.506AlaLys: 3.506 ± 0.086
15.704AlaLeu: 15.704 ± 0.202
3.532AlaMet: 3.532 ± 0.07
3.137AlaAsn: 3.137 ± 0.069
6.414AlaPro: 6.414 ± 0.108
4.546AlaGln: 4.546 ± 0.093
11.459AlaArg: 11.459 ± 0.17
6.353AlaSer: 6.353 ± 0.101
5.794AlaThr: 5.794 ± 0.102
7.852AlaVal: 7.852 ± 0.118
2.044AlaTrp: 2.044 ± 0.052
2.476AlaTyr: 2.476 ± 0.06
0.0AlaXaa: 0.0 ± 0.0
Cys
0.979CysAla: 0.979 ± 0.042
0.103CysCys: 0.103 ± 0.012
0.479CysAsp: 0.479 ± 0.025
0.482CysGlu: 0.482 ± 0.025
0.268CysPhe: 0.268 ± 0.02
0.883CysGly: 0.883 ± 0.037
0.223CysHis: 0.223 ± 0.018
0.355CysIle: 0.355 ± 0.023
0.203CysLys: 0.203 ± 0.017
0.788CysLeu: 0.788 ± 0.031
0.158CysMet: 0.158 ± 0.013
0.195CysAsn: 0.195 ± 0.016
0.424CysPro: 0.424 ± 0.022
0.245CysGln: 0.245 ± 0.017
0.635CysArg: 0.635 ± 0.031
0.361CysSer: 0.361 ± 0.023
0.365CysThr: 0.365 ± 0.02
0.646CysVal: 0.646 ± 0.028
0.101CysTrp: 0.101 ± 0.011
0.178CysTyr: 0.178 ± 0.017
0.0CysXaa: 0.0 ± 0.0
Asp
8.701AspAla: 8.701 ± 0.119
0.39AspCys: 0.39 ± 0.021
3.317AspAsp: 3.317 ± 0.082
3.511AspGlu: 3.511 ± 0.068
2.121AspPhe: 2.121 ± 0.055
5.627AspGly: 5.627 ± 0.112
1.146AspHis: 1.146 ± 0.04
2.736AspIle: 2.736 ± 0.06
1.596AspLys: 1.596 ± 0.044
5.058AspLeu: 5.058 ± 0.081
1.135AspMet: 1.135 ± 0.042
1.399AspAsn: 1.399 ± 0.047
3.276AspPro: 3.276 ± 0.066
1.422AspGln: 1.422 ± 0.04
3.62AspArg: 3.62 ± 0.067
2.426AspSer: 2.426 ± 0.049
2.884AspThr: 2.884 ± 0.069
3.825AspVal: 3.825 ± 0.08
1.129AspTrp: 1.129 ± 0.049
1.685AspTyr: 1.685 ± 0.048
0.0AspXaa: 0.0 ± 0.0
Glu
7.987GluAla: 7.987 ± 0.144
0.413GluCys: 0.413 ± 0.022
2.549GluAsp: 2.549 ± 0.066
2.527GluGlu: 2.527 ± 0.073
1.737GluPhe: 1.737 ± 0.05
4.262GluGly: 4.262 ± 0.073
1.411GluHis: 1.411 ± 0.042
2.776GluIle: 2.776 ± 0.057
1.882GluLys: 1.882 ± 0.056
5.711GluLeu: 5.711 ± 0.094
1.392GluMet: 1.392 ± 0.043
1.503GluAsn: 1.503 ± 0.04
2.399GluPro: 2.399 ± 0.061
2.116GluGln: 2.116 ± 0.056
5.683GluArg: 5.683 ± 0.097
2.816GluSer: 2.816 ± 0.073
3.164GluThr: 3.164 ± 0.063
4.08GluVal: 4.08 ± 0.083
0.791GluTrp: 0.791 ± 0.029
1.278GluTyr: 1.278 ± 0.041
0.0GluXaa: 0.0 ± 0.0
Phe
4.192PheAla: 4.192 ± 0.078
0.297PheCys: 0.297 ± 0.017
2.783PheAsp: 2.783 ± 0.06
1.951PheGlu: 1.951 ± 0.043
1.168PhePhe: 1.168 ± 0.048
3.425PheGly: 3.425 ± 0.067
0.842PheHis: 0.842 ± 0.035
1.411PheIle: 1.411 ± 0.045
0.947PheLys: 0.947 ± 0.035
2.901PheLeu: 2.901 ± 0.062
0.689PheMet: 0.689 ± 0.033
1.183PheAsn: 1.183 ± 0.04
1.419PhePro: 1.419 ± 0.04
0.955PheGln: 0.955 ± 0.032
2.245PheArg: 2.245 ± 0.056
1.939PheSer: 1.939 ± 0.055
1.583PheThr: 1.583 ± 0.047
2.452PheVal: 2.452 ± 0.05
0.499PheTrp: 0.499 ± 0.028
0.731PheTyr: 0.731 ± 0.029
0.0PheXaa: 0.0 ± 0.0
Gly
9.142GlyAla: 9.142 ± 0.117
0.791GlyCys: 0.791 ± 0.032
4.916GlyAsp: 4.916 ± 0.097
5.744GlyGlu: 5.744 ± 0.088
3.462GlyPhe: 3.462 ± 0.074
7.897GlyGly: 7.897 ± 0.17
2.229GlyHis: 2.229 ± 0.057
4.579GlyIle: 4.579 ± 0.079
3.328GlyLys: 3.328 ± 0.079
8.826GlyLeu: 8.826 ± 0.122
2.476GlyMet: 2.476 ± 0.06
2.707GlyAsn: 2.707 ± 0.122
3.038GlyPro: 3.038 ± 0.06
3.456GlyGln: 3.456 ± 0.086
7.014GlyArg: 7.014 ± 0.14
4.08GlySer: 4.08 ± 0.09
3.816GlyThr: 3.816 ± 0.08
6.341GlyVal: 6.341 ± 0.089
1.805GlyTrp: 1.805 ± 0.059
2.356GlyTyr: 2.356 ± 0.056
0.0GlyXaa: 0.0 ± 0.0
His
3.345HisAla: 3.345 ± 0.076
0.261HisCys: 0.261 ± 0.017
1.424HisAsp: 1.424 ± 0.043
1.208HisGlu: 1.208 ± 0.043
0.811HisPhe: 0.811 ± 0.034
2.43HisGly: 2.43 ± 0.055
0.637HisHis: 0.637 ± 0.032
0.742HisIle: 0.742 ± 0.028
0.47HisLys: 0.47 ± 0.025
2.227HisLeu: 2.227 ± 0.054
0.406HisMet: 0.406 ± 0.024
0.475HisAsn: 0.475 ± 0.026
1.626HisPro: 1.626 ± 0.046
0.656HisGln: 0.656 ± 0.027
1.937HisArg: 1.937 ± 0.055
0.944HisSer: 0.944 ± 0.034
1.012HisThr: 1.012 ± 0.037
1.574HisVal: 1.574 ± 0.04
0.507HisTrp: 0.507 ± 0.026
0.678HisTyr: 0.678 ± 0.03
0.0HisXaa: 0.0 ± 0.0
Ile
6.672IleAla: 6.672 ± 0.112
0.36IleCys: 0.36 ± 0.022
3.574IleAsp: 3.574 ± 0.067
3.43IleGlu: 3.43 ± 0.07
1.085IlePhe: 1.085 ± 0.038
4.749IleGly: 4.749 ± 0.083
0.979IleHis: 0.979 ± 0.038
1.363IleIle: 1.363 ± 0.047
1.17IleLys: 1.17 ± 0.044
3.235IleLeu: 3.235 ± 0.071
0.54IleMet: 0.54 ± 0.027
1.235IleAsn: 1.235 ± 0.041
2.205IlePro: 2.205 ± 0.053
1.155IleGln: 1.155 ± 0.043
2.901IleArg: 2.901 ± 0.061
2.093IleSer: 2.093 ± 0.054
2.065IleThr: 2.065 ± 0.056
3.41IleVal: 3.41 ± 0.082
0.459IleTrp: 0.459 ± 0.023
0.852IleTyr: 0.852 ± 0.032
0.0IleXaa: 0.0 ± 0.0
Lys
3.503LysAla: 3.503 ± 0.086
0.16LysCys: 0.16 ± 0.015
1.436LysAsp: 1.436 ± 0.048
1.309LysGlu: 1.309 ± 0.049
0.852LysPhe: 0.852 ± 0.03
2.151LysGly: 2.151 ± 0.06
0.658LysHis: 0.658 ± 0.029
1.284LysIle: 1.284 ± 0.04
1.132LysLys: 1.132 ± 0.066
3.244LysLeu: 3.244 ± 0.07
0.627LysMet: 0.627 ± 0.032
0.82LysAsn: 0.82 ± 0.034
2.154LysPro: 2.154 ± 0.06
1.13LysGln: 1.13 ± 0.04
2.497LysArg: 2.497 ± 0.064
1.526LysSer: 1.526 ± 0.041
1.721LysThr: 1.721 ± 0.048
2.101LysVal: 2.101 ± 0.063
0.36LysTrp: 0.36 ± 0.022
0.651LysTyr: 0.651 ± 0.028
0.0LysXaa: 0.0 ± 0.0
Leu
15.702LeuAla: 15.702 ± 0.213
0.915LeuCys: 0.915 ± 0.036
6.723LeuAsp: 6.723 ± 0.104
5.271LeuGlu: 5.271 ± 0.092
3.4LeuPhe: 3.4 ± 0.082
8.855LeuGly: 8.855 ± 0.1
2.387LeuHis: 2.387 ± 0.057
4.079LeuIle: 4.079 ± 0.085
3.352LeuLys: 3.352 ± 0.062
11.27LeuLeu: 11.27 ± 0.193
2.228LeuMet: 2.228 ± 0.058
2.463LeuAsn: 2.463 ± 0.063
6.675LeuPro: 6.675 ± 0.105
3.158LeuGln: 3.158 ± 0.067
8.964LeuArg: 8.964 ± 0.147
5.848LeuSer: 5.848 ± 0.088
4.797LeuThr: 4.797 ± 0.083
6.85LeuVal: 6.85 ± 0.108
1.299LeuTrp: 1.299 ± 0.047
2.089LeuTyr: 2.089 ± 0.057
0.0LeuXaa: 0.0 ± 0.0
Met
2.889MetAla: 2.889 ± 0.066
0.155MetCys: 0.155 ± 0.018
1.076MetAsp: 1.076 ± 0.039
1.085MetGlu: 1.085 ± 0.035
0.694MetPhe: 0.694 ± 0.031
1.636MetGly: 1.636 ± 0.047
0.482MetHis: 0.482 ± 0.023
0.901MetIle: 0.901 ± 0.041
0.957MetLys: 0.957 ± 0.034
2.611MetLeu: 2.611 ± 0.063
0.506MetMet: 0.506 ± 0.026
0.818MetAsn: 0.818 ± 0.031
1.572MetPro: 1.572 ± 0.045
0.834MetGln: 0.834 ± 0.031
2.133MetArg: 2.133 ± 0.053
1.638MetSer: 1.638 ± 0.036
1.349MetThr: 1.349 ± 0.041
1.426MetVal: 1.426 ± 0.049
0.207MetTrp: 0.207 ± 0.017
0.375MetTyr: 0.375 ± 0.02
0.0MetXaa: 0.0 ± 0.0
Asn
3.576AsnAla: 3.576 ± 0.073
0.216AsnCys: 0.216 ± 0.017
1.526AsnAsp: 1.526 ± 0.071
1.279AsnGlu: 1.279 ± 0.044
0.869AsnPhe: 0.869 ± 0.033
2.487AsnGly: 2.487 ± 0.082
0.511AsnHis: 0.511 ± 0.026
1.176AsnIle: 1.176 ± 0.04
0.689AsnLys: 0.689 ± 0.035
2.55AsnLeu: 2.55 ± 0.07
0.477AsnMet: 0.477 ± 0.026
0.647AsnAsn: 0.647 ± 0.034
1.854AsnPro: 1.854 ± 0.057
0.829AsnGln: 0.829 ± 0.039
1.875AsnArg: 1.875 ± 0.053
1.11AsnSer: 1.11 ± 0.047
1.369AsnThr: 1.369 ± 0.048
1.815AsnVal: 1.815 ± 0.06
0.409AsnTrp: 0.409 ± 0.021
0.65AsnTyr: 0.65 ± 0.033
0.0AsnXaa: 0.0 ± 0.0
Pro
7.623ProAla: 7.623 ± 0.126
0.325ProCys: 0.325 ± 0.021
3.424ProAsp: 3.424 ± 0.059
3.45ProGlu: 3.45 ± 0.066
1.668ProPhe: 1.668 ± 0.047
5.172ProGly: 5.172 ± 0.08
1.173ProHis: 1.173 ± 0.037
1.986ProIle: 1.986 ± 0.049
1.58ProLys: 1.58 ± 0.041
5.331ProLeu: 5.331 ± 0.084
1.378ProMet: 1.378 ± 0.045
1.271ProAsn: 1.271 ± 0.043
3.475ProPro: 3.475 ± 0.097
1.661ProGln: 1.661 ± 0.04
3.904ProArg: 3.904 ± 0.068
2.472ProSer: 2.472 ± 0.059
2.278ProThr: 2.278 ± 0.054
4.191ProVal: 4.191 ± 0.084
0.852ProTrp: 0.852 ± 0.035
1.17ProTyr: 1.17 ± 0.035
0.0ProXaa: 0.0 ± 0.0
Gln
4.828GlnAla: 4.828 ± 0.082
0.246GlnCys: 0.246 ± 0.016
1.313GlnAsp: 1.313 ± 0.042
1.293GlnGlu: 1.293 ± 0.045
1.052GlnPhe: 1.052 ± 0.032
2.499GlnGly: 2.499 ± 0.055
0.705GlnHis: 0.705 ± 0.032
1.349GlnIle: 1.349 ± 0.046
0.984GlnLys: 0.984 ± 0.036
3.661GlnLeu: 3.661 ± 0.079
0.787GlnMet: 0.787 ± 0.031
0.774GlnAsn: 0.774 ± 0.034
2.121GlnPro: 2.121 ± 0.055
1.665GlnGln: 1.665 ± 0.065
3.449GlnArg: 3.449 ± 0.077
1.721GlnSer: 1.721 ± 0.049
1.653GlnThr: 1.653 ± 0.055
2.54GlnVal: 2.54 ± 0.053
0.629GlnTrp: 0.629 ± 0.027
0.771GlnTyr: 0.771 ± 0.032
0.0GlnXaa: 0.0 ± 0.0
Arg
9.522ArgAla: 9.522 ± 0.136
0.595ArgCys: 0.595 ± 0.031
4.795ArgAsp: 4.795 ± 0.085
5.56ArgGlu: 5.56 ± 0.104
3.171ArgPhe: 3.171 ± 0.07
6.045ArgGly: 6.045 ± 0.11
2.454ArgHis: 2.454 ± 0.065
4.381ArgIle: 4.381 ± 0.07
2.141ArgLys: 2.141 ± 0.056
9.215ArgLeu: 9.215 ± 0.126
2.205ArgMet: 2.205 ± 0.047
2.004ArgAsn: 2.004 ± 0.052
3.938ArgPro: 3.938 ± 0.094
3.48ArgGln: 3.48 ± 0.069
6.67ArgArg: 6.67 ± 0.117
3.195ArgSer: 3.195 ± 0.071
3.293ArgThr: 3.293 ± 0.07
5.542ArgVal: 5.542 ± 0.097
1.477ArgTrp: 1.477 ± 0.044
2.389ArgTyr: 2.389 ± 0.058
0.0ArgXaa: 0.0 ± 0.0
Ser
6.075SerAla: 6.075 ± 0.09
0.34SerCys: 0.34 ± 0.022
2.479SerAsp: 2.479 ± 0.057
2.366SerGlu: 2.366 ± 0.059
1.69SerPhe: 1.69 ± 0.052
5.103SerGly: 5.103 ± 0.106
1.169SerHis: 1.169 ± 0.043
2.173SerIle: 2.173 ± 0.054
1.36SerLys: 1.36 ± 0.038
5.202SerLeu: 5.202 ± 0.093
1.192SerMet: 1.192 ± 0.038
1.246SerAsn: 1.246 ± 0.044
2.942SerPro: 2.942 ± 0.059
1.693SerGln: 1.693 ± 0.049
3.865SerArg: 3.865 ± 0.074
2.453SerSer: 2.453 ± 0.069
2.522SerThr: 2.522 ± 0.06
3.326SerVal: 3.326 ± 0.068
0.689SerTrp: 0.689 ± 0.029
1.146SerTyr: 1.146 ± 0.04
0.0SerXaa: 0.0 ± 0.0
Thr
5.358ThrAla: 5.358 ± 0.086
0.372ThrCys: 0.372 ± 0.021
2.403ThrAsp: 2.403 ± 0.06
2.193ThrGlu: 2.193 ± 0.057
1.434ThrPhe: 1.434 ± 0.047
4.588ThrGly: 4.588 ± 0.095
1.235ThrHis: 1.235 ± 0.038
1.875ThrIle: 1.875 ± 0.051
0.922ThrLys: 0.922 ± 0.031
6.252ThrLeu: 6.252 ± 0.102
1.002ThrMet: 1.002 ± 0.039
1.018ThrAsn: 1.018 ± 0.043
3.43ThrPro: 3.43 ± 0.071
1.516ThrGln: 1.516 ± 0.05
3.946ThrArg: 3.946 ± 0.082
2.119ThrSer: 2.119 ± 0.059
2.31ThrThr: 2.31 ± 0.068
3.578ThrVal: 3.578 ± 0.085
0.654ThrTrp: 0.654 ± 0.035
1.091ThrTyr: 1.091 ± 0.061
0.0ThrXaa: 0.0 ± 0.0
Val
8.817ValAla: 8.817 ± 0.119
0.595ValCys: 0.595 ± 0.027
4.02ValAsp: 4.02 ± 0.081
4.392ValGlu: 4.392 ± 0.082
2.475ValPhe: 2.475 ± 0.055
5.132ValGly: 5.132 ± 0.089
1.355ValHis: 1.355 ± 0.034
3.411ValIle: 3.411 ± 0.07
2.069ValLys: 2.069 ± 0.062
7.671ValLeu: 7.671 ± 0.119
1.723ValMet: 1.723 ± 0.047
1.953ValAsn: 1.953 ± 0.066
3.479ValPro: 3.479 ± 0.06
1.942ValGln: 1.942 ± 0.058
5.23ValArg: 5.23 ± 0.098
3.897ValSer: 3.897 ± 0.078
3.511ValThr: 3.511 ± 0.071
5.303ValVal: 5.303 ± 0.117
0.935ValTrp: 0.935 ± 0.034
1.412ValTyr: 1.412 ± 0.044
0.0ValXaa: 0.0 ± 0.0
Trp
1.35TrpAla: 1.35 ± 0.04
0.158TrpCys: 0.158 ± 0.013
0.58TrpAsp: 0.58 ± 0.025
0.54TrpGlu: 0.54 ± 0.026
0.594TrpPhe: 0.594 ± 0.027
0.904TrpGly: 0.904 ± 0.038
0.389TrpHis: 0.389 ± 0.022
0.618TrpIle: 0.618 ± 0.028
0.474TrpLys: 0.474 ± 0.025
2.488TrpLeu: 2.488 ± 0.06
0.47TrpMet: 0.47 ± 0.028
0.464TrpAsn: 0.464 ± 0.023
0.831TrpPro: 0.831 ± 0.036
0.745TrpGln: 0.745 ± 0.034
1.854TrpArg: 1.854 ± 0.053
0.858TrpSer: 0.858 ± 0.03
0.658TrpThr: 0.658 ± 0.031
0.934TrpVal: 0.934 ± 0.034
0.305TrpTrp: 0.305 ± 0.026
0.339TrpTyr: 0.339 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.833TyrAla: 2.833 ± 0.06
0.173TyrCys: 0.173 ± 0.014
1.511TyrAsp: 1.511 ± 0.109
1.078TyrGlu: 1.078 ± 0.037
0.919TyrPhe: 0.919 ± 0.033
2.182TyrGly: 2.182 ± 0.065
0.523TyrHis: 0.523 ± 0.024
0.724TyrIle: 0.724 ± 0.032
0.553TyrLys: 0.553 ± 0.026
2.383TyrLeu: 2.383 ± 0.055
0.421TyrMet: 0.421 ± 0.022
0.604TyrAsn: 0.604 ± 0.034
1.112TyrPro: 1.112 ± 0.035
0.837TyrGln: 0.837 ± 0.031
2.191TyrArg: 2.191 ± 0.056
1.183TyrSer: 1.183 ± 0.048
1.151TyrThr: 1.151 ± 0.043
1.537TyrVal: 1.537 ± 0.049
0.404TyrTrp: 0.404 ± 0.02
0.577TyrTyr: 0.577 ± 0.026
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2550 proteins (850476 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski