Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae; Pyrococcus; Pyrococcus furiosus

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2045 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P58814|APGM_PYRFU 2 3-bisphosphoglycerate-independent phosphoglycerate mutase OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) OX=186497 GN=apgM PE=1 SV=1
MM1 pKa = 7.17AWKK4 pKa = 10.59VSVDD8 pKa = 3.04QDD10 pKa = 3.76TCIGDD15 pKa = 4.75AICASLCPDD24 pKa = 3.27VFEE27 pKa = 5.34MNDD30 pKa = 3.19EE31 pKa = 4.41GKK33 pKa = 10.0AQPKK37 pKa = 10.02VEE39 pKa = 4.3VIEE42 pKa = 5.45DD43 pKa = 3.65EE44 pKa = 4.3EE45 pKa = 5.12LYY47 pKa = 11.11NCAKK51 pKa = 10.1EE52 pKa = 4.73AMEE55 pKa = 4.75ACPVSAITIEE65 pKa = 4.18EE66 pKa = 4.1AA67 pKa = 3.57

Molecular weight: 7.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8U255|Q8U255_PYRFU Uncharacterized protein OS=Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) OX=186497 GN=PF0995 PE=4 SV=1
MM1 pKa = 7.48KK2 pKa = 10.23RR3 pKa = 11.84RR4 pKa = 11.84PRR6 pKa = 11.84KK7 pKa = 7.59WKK9 pKa = 10.02KK10 pKa = 8.58KK11 pKa = 10.27GRR13 pKa = 11.84MRR15 pKa = 11.84WKK17 pKa = 9.51WIKK20 pKa = 10.46KK21 pKa = 8.96RR22 pKa = 11.84IRR24 pKa = 11.84RR25 pKa = 11.84LKK27 pKa = 9.33RR28 pKa = 11.84QRR30 pKa = 11.84KK31 pKa = 8.4KK32 pKa = 10.86EE33 pKa = 3.77RR34 pKa = 11.84GLII37 pKa = 3.84

Molecular weight: 5.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2045

0

2045

577545

21

1740

282.4

31.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.579 ± 0.051

0.591 ± 0.016

4.385 ± 0.033

8.926 ± 0.069

4.411 ± 0.038

7.154 ± 0.046

1.512 ± 0.019

8.725 ± 0.049

8.098 ± 0.051

10.104 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.249 ± 0.019

3.481 ± 0.03

4.29 ± 0.033

1.746 ± 0.021

5.314 ± 0.04

4.924 ± 0.046

4.417 ± 0.038

7.869 ± 0.045

1.22 ± 0.021

4.006 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski