Amino acid dipepetide frequency for Pseudothermotoga lettingae (strain ATCC BAA-301 / DSM 14385 / NBRC 107922 / TMO) (Thermotoga lettingae)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.669AlaAla: 4.669 ± 0.12
0.628AlaCys: 0.628 ± 0.036
2.845AlaAsp: 2.845 ± 0.058
3.807AlaGlu: 3.807 ± 0.09
3.194AlaPhe: 3.194 ± 0.079
4.719AlaGly: 4.719 ± 0.086
0.99AlaHis: 0.99 ± 0.041
5.847AlaIle: 5.847 ± 0.116
4.661AlaLys: 4.661 ± 0.102
6.968AlaLeu: 6.968 ± 0.1
1.84AlaMet: 1.84 ± 0.061
2.338AlaAsn: 2.338 ± 0.062
1.746AlaPro: 1.746 ± 0.057
1.924AlaGln: 1.924 ± 0.056
3.206AlaArg: 3.206 ± 0.075
3.632AlaSer: 3.632 ± 0.086
2.989AlaThr: 2.989 ± 0.067
5.274AlaVal: 5.274 ± 0.115
0.58AlaTrp: 0.58 ± 0.028
2.194AlaTyr: 2.194 ± 0.048
0.0AlaXaa: 0.0 ± 0.0
Cys
0.549CysAla: 0.549 ± 0.029
0.097CysCys: 0.097 ± 0.013
0.55CysAsp: 0.55 ± 0.028
0.688CysGlu: 0.688 ± 0.033
0.434CysPhe: 0.434 ± 0.03
0.951CysGly: 0.951 ± 0.041
0.214CysHis: 0.214 ± 0.017
0.631CysIle: 0.631 ± 0.035
0.588CysLys: 0.588 ± 0.031
0.717CysLeu: 0.717 ± 0.039
0.19CysMet: 0.19 ± 0.016
0.393CysAsn: 0.393 ± 0.025
0.504CysPro: 0.504 ± 0.029
0.244CysGln: 0.244 ± 0.021
0.399CysArg: 0.399 ± 0.028
0.673CysSer: 0.673 ± 0.034
0.434CysThr: 0.434 ± 0.027
0.788CysVal: 0.788 ± 0.036
0.123CysTrp: 0.123 ± 0.013
0.329CysTyr: 0.329 ± 0.024
0.0CysXaa: 0.0 ± 0.0
Asp
2.721AspAla: 2.721 ± 0.07
0.383AspCys: 0.383 ± 0.026
2.091AspAsp: 2.091 ± 0.056
4.203AspGlu: 4.203 ± 0.091
3.116AspPhe: 3.116 ± 0.072
3.426AspGly: 3.426 ± 0.073
0.835AspHis: 0.835 ± 0.038
4.524AspIle: 4.524 ± 0.092
2.915AspLys: 2.915 ± 0.073
5.557AspLeu: 5.557 ± 0.107
1.171AspMet: 1.171 ± 0.046
1.608AspAsn: 1.608 ± 0.05
2.329AspPro: 2.329 ± 0.058
1.464AspGln: 1.464 ± 0.052
2.319AspArg: 2.319 ± 0.065
2.836AspSer: 2.836 ± 0.072
1.931AspThr: 1.931 ± 0.053
3.897AspVal: 3.897 ± 0.078
0.582AspTrp: 0.582 ± 0.035
2.054AspTyr: 2.054 ± 0.058
0.0AspXaa: 0.0 ± 0.0
Glu
4.076GluAla: 4.076 ± 0.083
0.417GluCys: 0.417 ± 0.028
3.291GluAsp: 3.291 ± 0.073
5.759GluGlu: 5.759 ± 0.106
3.092GluPhe: 3.092 ± 0.082
3.493GluGly: 3.493 ± 0.068
1.007GluHis: 1.007 ± 0.04
6.936GluIle: 6.936 ± 0.116
8.004GluLys: 8.004 ± 0.12
6.752GluLeu: 6.752 ± 0.121
1.841GluMet: 1.841 ± 0.048
4.187GluAsn: 4.187 ± 0.074
1.93GluPro: 1.93 ± 0.056
2.165GluGln: 2.165 ± 0.058
3.382GluArg: 3.382 ± 0.073
3.545GluSer: 3.545 ± 0.076
2.942GluThr: 2.942 ± 0.07
4.205GluVal: 4.205 ± 0.09
0.651GluTrp: 0.651 ± 0.03
2.518GluTyr: 2.518 ± 0.062
0.0GluXaa: 0.0 ± 0.0
Phe
3.445PheAla: 3.445 ± 0.076
0.555PheCys: 0.555 ± 0.028
2.959PheAsp: 2.959 ± 0.073
3.698PheGlu: 3.698 ± 0.077
2.912PhePhe: 2.912 ± 0.086
3.636PheGly: 3.636 ± 0.078
0.851PheHis: 0.851 ± 0.039
3.527PheIle: 3.527 ± 0.086
2.996PheLys: 2.996 ± 0.066
5.78PheLeu: 5.78 ± 0.13
1.282PheMet: 1.282 ± 0.044
1.901PheAsn: 1.901 ± 0.059
1.819PhePro: 1.819 ± 0.054
1.372PheGln: 1.372 ± 0.04
1.858PheArg: 1.858 ± 0.048
4.39PheSer: 4.39 ± 0.094
2.314PheThr: 2.314 ± 0.065
4.114PheVal: 4.114 ± 0.096
0.571PheTrp: 0.571 ± 0.035
1.888PheTyr: 1.888 ± 0.056
0.0PheXaa: 0.0 ± 0.0
Gly
4.522GlyAla: 4.522 ± 0.099
0.76GlyCys: 0.76 ± 0.034
2.749GlyAsp: 2.749 ± 0.062
4.187GlyGlu: 4.187 ± 0.074
3.648GlyPhe: 3.648 ± 0.081
4.346GlyGly: 4.346 ± 0.089
1.062GlyHis: 1.062 ± 0.041
5.916GlyIle: 5.916 ± 0.096
5.53GlyLys: 5.53 ± 0.1
5.874GlyLeu: 5.874 ± 0.102
1.819GlyMet: 1.819 ± 0.055
2.374GlyAsn: 2.374 ± 0.062
1.798GlyPro: 1.798 ± 0.044
1.623GlyGln: 1.623 ± 0.051
2.719GlyArg: 2.719 ± 0.058
3.678GlySer: 3.678 ± 0.083
3.446GlyThr: 3.446 ± 0.074
5.107GlyVal: 5.107 ± 0.083
0.778GlyTrp: 0.778 ± 0.037
2.85GlyTyr: 2.85 ± 0.067
0.0GlyXaa: 0.0 ± 0.0
His
0.96HisAla: 0.96 ± 0.045
0.217HisCys: 0.217 ± 0.018
0.823HisAsp: 0.823 ± 0.041
1.053HisGlu: 1.053 ± 0.042
0.748HisPhe: 0.748 ± 0.036
1.245HisGly: 1.245 ± 0.053
0.319HisHis: 0.319 ± 0.024
1.328HisIle: 1.328 ± 0.048
0.812HisLys: 0.812 ± 0.036
1.598HisLeu: 1.598 ± 0.049
0.389HisMet: 0.389 ± 0.021
0.609HisAsn: 0.609 ± 0.031
0.951HisPro: 0.951 ± 0.035
0.455HisGln: 0.455 ± 0.027
0.797HisArg: 0.797 ± 0.035
0.986HisSer: 0.986 ± 0.038
0.773HisThr: 0.773 ± 0.033
1.168HisVal: 1.168 ± 0.047
0.154HisTrp: 0.154 ± 0.015
0.571HisTyr: 0.571 ± 0.029
0.0HisXaa: 0.0 ± 0.0
Ile
6.369IleAla: 6.369 ± 0.106
0.972IleCys: 0.972 ± 0.037
5.279IleAsp: 5.279 ± 0.093
6.305IleGlu: 6.305 ± 0.121
4.827IlePhe: 4.827 ± 0.099
5.435IleGly: 5.435 ± 0.098
1.322IleHis: 1.322 ± 0.049
5.94IleIle: 5.94 ± 0.105
5.572IleLys: 5.572 ± 0.092
8.556IleLeu: 8.556 ± 0.12
1.856IleMet: 1.856 ± 0.049
3.436IleAsn: 3.436 ± 0.071
3.822IlePro: 3.822 ± 0.074
2.106IleGln: 2.106 ± 0.06
3.402IleArg: 3.402 ± 0.076
6.438IleSer: 6.438 ± 0.105
4.254IleThr: 4.254 ± 0.077
6.82IleVal: 6.82 ± 0.103
0.763IleTrp: 0.763 ± 0.038
2.968IleTyr: 2.968 ± 0.072
0.0IleXaa: 0.0 ± 0.0
Lys
4.423LysAla: 4.423 ± 0.089
0.555LysCys: 0.555 ± 0.033
3.888LysAsp: 3.888 ± 0.08
5.384LysGlu: 5.384 ± 0.098
2.838LysPhe: 2.838 ± 0.065
3.964LysGly: 3.964 ± 0.083
1.105LysHis: 1.105 ± 0.043
8.172LysIle: 8.172 ± 0.113
6.399LysLys: 6.399 ± 0.106
7.087LysLeu: 7.087 ± 0.122
2.144LysMet: 2.144 ± 0.054
4.124LysAsn: 4.124 ± 0.089
2.675LysPro: 2.675 ± 0.055
2.162LysGln: 2.162 ± 0.064
3.409LysArg: 3.409 ± 0.085
4.172LysSer: 4.172 ± 0.08
3.791LysThr: 3.791 ± 0.083
5.095LysVal: 5.095 ± 0.092
0.693LysTrp: 0.693 ± 0.034
2.788LysTyr: 2.788 ± 0.074
0.0LysXaa: 0.0 ± 0.0
Leu
6.164LeuAla: 6.164 ± 0.113
1.05LeuCys: 1.05 ± 0.039
4.576LeuAsp: 4.576 ± 0.097
6.594LeuGlu: 6.594 ± 0.116
4.761LeuPhe: 4.761 ± 0.105
5.958LeuGly: 5.958 ± 0.107
1.406LeuHis: 1.406 ± 0.053
8.619LeuIle: 8.619 ± 0.131
8.262LeuLys: 8.262 ± 0.131
9.681LeuLeu: 9.681 ± 0.144
2.564LeuMet: 2.564 ± 0.061
4.528LeuAsn: 4.528 ± 0.089
3.873LeuPro: 3.873 ± 0.082
2.829LeuGln: 2.829 ± 0.069
5.028LeuArg: 5.028 ± 0.088
8.428LeuSer: 8.428 ± 0.134
4.817LeuThr: 4.817 ± 0.094
6.227LeuVal: 6.227 ± 0.11
0.892LeuTrp: 0.892 ± 0.041
3.126LeuTyr: 3.126 ± 0.068
0.0LeuXaa: 0.0 ± 0.0
Met
1.759MetAla: 1.759 ± 0.054
0.203MetCys: 0.203 ± 0.019
1.067MetAsp: 1.067 ± 0.044
1.427MetGlu: 1.427 ± 0.048
0.972MetPhe: 0.972 ± 0.04
1.701MetGly: 1.701 ± 0.049
0.311MetHis: 0.311 ± 0.021
2.381MetIle: 2.381 ± 0.061
2.579MetLys: 2.579 ± 0.059
2.233MetLeu: 2.233 ± 0.055
0.658MetMet: 0.658 ± 0.033
1.438MetAsn: 1.438 ± 0.05
0.893MetPro: 0.893 ± 0.035
0.628MetGln: 0.628 ± 0.027
1.49MetArg: 1.49 ± 0.051
1.529MetSer: 1.529 ± 0.049
1.219MetThr: 1.219 ± 0.051
1.856MetVal: 1.856 ± 0.058
0.221MetTrp: 0.221 ± 0.017
0.841MetTyr: 0.841 ± 0.038
0.0MetXaa: 0.0 ± 0.0
Asn
2.621AsnAla: 2.621 ± 0.061
0.489AsnCys: 0.489 ± 0.028
1.987AsnAsp: 1.987 ± 0.048
2.76AsnGlu: 2.76 ± 0.056
2.407AsnPhe: 2.407 ± 0.059
2.829AsnGly: 2.829 ± 0.061
0.708AsnHis: 0.708 ± 0.035
3.792AsnIle: 3.792 ± 0.076
2.302AsnLys: 2.302 ± 0.06
4.966AsnLeu: 4.966 ± 0.084
1.09AsnMet: 1.09 ± 0.038
1.707AsnAsn: 1.707 ± 0.054
2.206AsnPro: 2.206 ± 0.066
1.316AsnGln: 1.316 ± 0.049
1.817AsnArg: 1.817 ± 0.055
2.55AsnSer: 2.55 ± 0.068
1.916AsnThr: 1.916 ± 0.048
3.249AsnVal: 3.249 ± 0.074
0.562AsnTrp: 0.562 ± 0.029
1.732AsnTyr: 1.732 ± 0.056
0.0AsnXaa: 0.0 ± 0.0
Pro
2.381ProAla: 2.381 ± 0.061
0.272ProCys: 0.272 ± 0.017
2.349ProAsp: 2.349 ± 0.066
3.022ProGlu: 3.022 ± 0.068
2.094ProPhe: 2.094 ± 0.059
2.503ProGly: 2.503 ± 0.072
0.702ProHis: 0.702 ± 0.033
2.368ProIle: 2.368 ± 0.062
2.22ProLys: 2.22 ± 0.065
3.349ProLeu: 3.349 ± 0.078
0.832ProMet: 0.832 ± 0.04
1.439ProAsn: 1.439 ± 0.047
1.15ProPro: 1.15 ± 0.049
1.092ProGln: 1.092 ± 0.043
1.34ProArg: 1.34 ± 0.046
2.374ProSer: 2.374 ± 0.065
1.702ProThr: 1.702 ± 0.051
3.557ProVal: 3.557 ± 0.079
0.432ProTrp: 0.432 ± 0.027
1.518ProTyr: 1.518 ± 0.048
0.0ProXaa: 0.0 ± 0.0
Gln
1.741GlnAla: 1.741 ± 0.055
0.156GlnCys: 0.156 ± 0.016
1.239GlnAsp: 1.239 ± 0.055
1.876GlnGlu: 1.876 ± 0.058
1.298GlnPhe: 1.298 ± 0.045
1.312GlnGly: 1.312 ± 0.048
0.441GlnHis: 0.441 ± 0.027
3.279GlnIle: 3.279 ± 0.07
2.512GlnLys: 2.512 ± 0.069
2.903GlnLeu: 2.903 ± 0.068
0.92GlnMet: 0.92 ± 0.033
1.466GlnAsn: 1.466 ± 0.054
0.96GlnPro: 0.96 ± 0.041
1.086GlnGln: 1.086 ± 0.045
1.366GlnArg: 1.366 ± 0.041
1.632GlnSer: 1.632 ± 0.049
1.379GlnThr: 1.379 ± 0.05
1.846GlnVal: 1.846 ± 0.05
0.275GlnTrp: 0.275 ± 0.02
0.917GlnTyr: 0.917 ± 0.039
0.0GlnXaa: 0.0 ± 0.0
Arg
2.637ArgAla: 2.637 ± 0.064
0.482ArgCys: 0.482 ± 0.03
2.075ArgAsp: 2.075 ± 0.058
3.497ArgGlu: 3.497 ± 0.087
2.506ArgPhe: 2.506 ± 0.065
2.585ArgGly: 2.585 ± 0.068
0.708ArgHis: 0.708 ± 0.037
4.202ArgIle: 4.202 ± 0.09
4.159ArgLys: 4.159 ± 0.082
3.987ArgLeu: 3.987 ± 0.086
1.24ArgMet: 1.24 ± 0.044
2.103ArgAsn: 2.103 ± 0.061
1.283ArgPro: 1.283 ± 0.045
1.319ArgGln: 1.319 ± 0.044
2.196ArgArg: 2.196 ± 0.072
2.571ArgSer: 2.571 ± 0.066
2.075ArgThr: 2.075 ± 0.055
3.095ArgVal: 3.095 ± 0.076
0.482ArgTrp: 0.482 ± 0.027
1.829ArgTyr: 1.829 ± 0.054
0.0ArgXaa: 0.0 ± 0.0
Ser
3.865SerAla: 3.865 ± 0.072
0.618SerCys: 0.618 ± 0.035
3.158SerAsp: 3.158 ± 0.061
4.414SerGlu: 4.414 ± 0.093
3.738SerPhe: 3.738 ± 0.09
4.956SerGly: 4.956 ± 0.097
1.122SerHis: 1.122 ± 0.04
5.33SerIle: 5.33 ± 0.085
4.609SerLys: 4.609 ± 0.095
6.407SerLeu: 6.407 ± 0.113
1.638SerMet: 1.638 ± 0.048
2.654SerAsn: 2.654 ± 0.071
2.22SerPro: 2.22 ± 0.055
2.214SerGln: 2.214 ± 0.065
2.829SerArg: 2.829 ± 0.068
4.338SerSer: 4.338 ± 0.102
3.065SerThr: 3.065 ± 0.069
4.761SerVal: 4.761 ± 0.086
0.682SerTrp: 0.682 ± 0.033
2.269SerTyr: 2.269 ± 0.056
0.0SerXaa: 0.0 ± 0.0
Thr
3.262ThrAla: 3.262 ± 0.078
0.405ThrCys: 0.405 ± 0.027
2.398ThrAsp: 2.398 ± 0.073
2.567ThrGlu: 2.567 ± 0.064
2.426ThrPhe: 2.426 ± 0.062
3.915ThrGly: 3.915 ± 0.073
0.865ThrHis: 0.865 ± 0.039
3.991ThrIle: 3.991 ± 0.072
2.655ThrLys: 2.655 ± 0.063
4.728ThrLeu: 4.728 ± 0.099
1.125ThrMet: 1.125 ± 0.045
1.786ThrAsn: 1.786 ± 0.047
2.01ThrPro: 2.01 ± 0.058
1.164ThrGln: 1.164 ± 0.047
1.907ThrArg: 1.907 ± 0.054
2.947ThrSer: 2.947 ± 0.067
2.577ThrThr: 2.577 ± 0.071
4.351ThrVal: 4.351 ± 0.082
0.458ThrTrp: 0.458 ± 0.027
1.708ThrTyr: 1.708 ± 0.055
0.0ThrXaa: 0.0 ± 0.0
Val
4.953ValAla: 4.953 ± 0.095
0.803ValCys: 0.803 ± 0.038
4.154ValAsp: 4.154 ± 0.082
5.749ValGlu: 5.749 ± 0.104
4.126ValPhe: 4.126 ± 0.085
4.546ValGly: 4.546 ± 0.094
1.147ValHis: 1.147 ± 0.041
6.075ValIle: 6.075 ± 0.097
5.298ValLys: 5.298 ± 0.097
7.593ValLeu: 7.593 ± 0.121
1.756ValMet: 1.756 ± 0.054
3.004ValAsn: 3.004 ± 0.069
2.684ValPro: 2.684 ± 0.055
2.006ValGln: 2.006 ± 0.051
3.2ValArg: 3.2 ± 0.068
5.04ValSer: 5.04 ± 0.082
3.331ValThr: 3.331 ± 0.073
6.47ValVal: 6.47 ± 0.111
0.637ValTrp: 0.637 ± 0.034
2.722ValTyr: 2.722 ± 0.061
0.0ValXaa: 0.0 ± 0.0
Trp
0.573TrpAla: 0.573 ± 0.034
0.103TrpCys: 0.103 ± 0.013
0.494TrpAsp: 0.494 ± 0.027
0.531TrpGlu: 0.531 ± 0.031
0.61TrpPhe: 0.61 ± 0.033
0.651TrpGly: 0.651 ± 0.032
0.196TrpHis: 0.196 ± 0.019
0.93TrpIle: 0.93 ± 0.037
0.776TrpLys: 0.776 ± 0.035
1.005TrpLeu: 1.005 ± 0.046
0.293TrpMet: 0.293 ± 0.021
0.568TrpAsn: 0.568 ± 0.029
0.292TrpPro: 0.292 ± 0.023
0.392TrpGln: 0.392 ± 0.026
0.431TrpArg: 0.431 ± 0.029
0.586TrpSer: 0.586 ± 0.035
0.441TrpThr: 0.441 ± 0.027
0.669TrpVal: 0.669 ± 0.033
0.187TrpTrp: 0.187 ± 0.018
0.401TrpTyr: 0.401 ± 0.025
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.359TyrAla: 2.359 ± 0.057
0.331TyrCys: 0.331 ± 0.02
1.993TyrAsp: 1.993 ± 0.057
2.673TyrGlu: 2.673 ± 0.064
2.121TyrPhe: 2.121 ± 0.059
2.619TyrGly: 2.619 ± 0.064
0.646TyrHis: 0.646 ± 0.034
2.654TyrIle: 2.654 ± 0.067
2.016TyrLys: 2.016 ± 0.059
3.639TyrLeu: 3.639 ± 0.089
0.757TyrMet: 0.757 ± 0.03
1.345TyrAsn: 1.345 ± 0.048
1.551TyrPro: 1.551 ± 0.052
1.046TyrGln: 1.046 ± 0.043
1.936TyrArg: 1.936 ± 0.049
2.54TyrSer: 2.54 ± 0.061
1.849TyrThr: 1.849 ± 0.054
2.743TyrVal: 2.743 ± 0.065
0.407TyrTrp: 0.407 ± 0.029
1.46TyrTyr: 1.46 ± 0.053
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2040 proteins (668507 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski