Halanaerobium sp. DL-01

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Halanaerobiales; Halanaerobiaceae; Halanaerobium; unclassified Halanaerobium

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2338 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A368Z9Z8|A0A368Z9Z8_9FIRM Chorismate mutase OS=Halanaerobium sp. DL-01 OX=1653064 GN=DER71_10154 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 10.38KK3 pKa = 10.38VITVVLVLSLLIFSGSVMAQDD24 pKa = 4.9YY25 pKa = 10.6LSQGQWEE32 pKa = 4.51IKK34 pKa = 10.6AGIDD38 pKa = 3.77SSGEE42 pKa = 3.96LEE44 pKa = 4.68DD45 pKa = 3.46WDD47 pKa = 3.51IDD49 pKa = 3.76RR50 pKa = 11.84GFSIAGEE57 pKa = 4.22YY58 pKa = 9.76KK59 pKa = 10.39FPYY62 pKa = 7.85TSRR65 pKa = 11.84WTLGGGLAYY74 pKa = 10.6QLDD77 pKa = 4.12RR78 pKa = 11.84EE79 pKa = 4.3VDD81 pKa = 3.24EE82 pKa = 5.13SGQDD86 pKa = 3.62GEE88 pKa = 4.39IGFVPFYY95 pKa = 11.39GLAQYY100 pKa = 10.74DD101 pKa = 3.83IANNPYY107 pKa = 10.39YY108 pKa = 10.68LVGHH112 pKa = 7.09LGFNSFRR119 pKa = 11.84TDD121 pKa = 2.69STFEE125 pKa = 4.16HH126 pKa = 6.87EE127 pKa = 4.66NDD129 pKa = 3.48VDD131 pKa = 3.88DD132 pKa = 4.35TSGGLYY138 pKa = 9.73YY139 pKa = 11.16ALGAGMEE146 pKa = 4.06FGEE149 pKa = 4.86RR150 pKa = 11.84YY151 pKa = 6.31TAEE154 pKa = 4.0MIYY157 pKa = 10.32SVNSGEE163 pKa = 3.89IEE165 pKa = 4.29YY166 pKa = 11.3NNGEE170 pKa = 4.32DD171 pKa = 4.7EE172 pKa = 4.26DD173 pKa = 4.27VEE175 pKa = 4.22YY176 pKa = 11.23DD177 pKa = 4.43KK178 pKa = 10.96FTLSIGMKK186 pKa = 9.98FF187 pKa = 3.17

Molecular weight: 20.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A368YWE9|A0A368YWE9_9FIRM Wyosine [tRNA(Phe)-imidazoG37] synthetase (Radical SAM superfamily) OS=Halanaerobium sp. DL-01 OX=1653064 GN=DER71_1209 PE=4 SV=1
MM1 pKa = 7.34ARR3 pKa = 11.84KK4 pKa = 9.46ALIEE8 pKa = 4.15KK9 pKa = 10.1ANKK12 pKa = 7.87EE13 pKa = 4.06PKK15 pKa = 9.9YY16 pKa = 8.13EE17 pKa = 3.89TRR19 pKa = 11.84RR20 pKa = 11.84VNRR23 pKa = 11.84CSRR26 pKa = 11.84CGRR29 pKa = 11.84VRR31 pKa = 11.84GYY33 pKa = 8.21MRR35 pKa = 11.84KK36 pKa = 9.32FDD38 pKa = 3.73LCRR41 pKa = 11.84ICFRR45 pKa = 11.84EE46 pKa = 3.96LAHH49 pKa = 6.84KK50 pKa = 11.01GEE52 pKa = 4.22IPGVQKK58 pKa = 11.13ASWW61 pKa = 3.07

Molecular weight: 7.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2338

0

2338

716680

28

1586

306.5

34.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.775 ± 0.048

0.705 ± 0.016

5.832 ± 0.04

8.122 ± 0.066

4.425 ± 0.045

6.668 ± 0.046

1.465 ± 0.021

9.109 ± 0.051

8.603 ± 0.059

9.57 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.48 ± 0.023

5.424 ± 0.046

3.175 ± 0.026

2.632 ± 0.025

3.931 ± 0.033

6.017 ± 0.033

4.401 ± 0.032

6.31 ± 0.046

0.692 ± 0.016

3.663 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski